(Circular) (Six-base) MAPSORT of: pFN21AB0450.seq Check: 6905 from: 1 to: 7294 pFN21AB0450 7294 bp With 182 enzymes: SgfI * March 28, 2012 12:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2178 2178 Size: 7294 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5104 278 Size: 53 83 186 936 3568 2468 Fragments arranged by size: 3568 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3776 4637 1937 Size: 1839 861 4594 Fragments arranged by size: 4594 1839 861 Acc65I G'GTAC_C Cuts at: 4615 4615 Size: 7294 AclI AA'CG_TT Cuts at: 1563 3298 5421 5794 1563 Size: 1735 2123 373 3063 Fragments arranged by size: 3063 2123 1735 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3607 3665 5357 6741 7009 1632 Size: 1975 58 1692 1384 268 1917 Fragments arranged by size: 1975 1917 1692 1384 268 58 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7097 AflIII A'CryG_T Cuts at: 1203 3101 3242 6193 7170 1203 Size: 1898 141 2951 977 1327 Fragments arranged by size: 2951 1898 1327 977 141 AgeI A'CCGG_T Cuts at: 6125 6125 Size: 7294 AhdI GACnn_n'nnGTC Cuts at: 6023 6023 Size: 7294 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7294 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2576 1184 Size: 1392 5902 AlwNI CAG_nnn'CTG Cuts at: 2262 5037 6609 2262 Size: 2775 1572 2947 Fragments arranged by size: 2947 2775 1572 ApaLI G'TGCA_C Cuts at: 5351 6507 5351 Size: 1156 6138 ApoI r'AATT_y Cuts at: 1493 2750 3180 3191 4603 4829 4981 7090 1493 Size: 1257 430 11 1412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 1257 430 226 152 11 AseI AT'TA_AT Cuts at: 160 1032 2501 3020 5848 160 Size: 872 1469 519 2828 1606 Fragments arranged by size: 2828 1606 1469 872 519 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7294 AvaI C'yCGr_G Cuts at: 1870 1943 4619 1870 Size: 73 2676 4545 Fragments arranged by size: 4545 2676 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2380 2413 2380 Size: 33 7261 BamHI G'GATC_C Cuts at: 4624 4991 4624 Size: 367 6927 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4615 6070 618 Size: 325 217 627 2828 1455 1842 Fragments arranged by size: 2828 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2021 4067 4613 1391 Size: 630 2046 546 4072 Fragments arranged by size: 4072 2046 630 546 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2297 4260 928 Size: 971 398 1963 3962 Fragments arranged by size: 3962 1963 971 398 BbvCI CC'TCA_GC Cuts at: 3924 3924 Size: 7294 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5485 5519 1903 Size: 34 3548 34 3678 Fragments arranged by size: 3678 3548 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4131 5186 6396 4131 Size: 1055 1210 5029 Fragments arranged by size: 5029 1210 1055 BclI T'GATC_A Cuts at: 1548 1548 Size: 7294 BfrBI ATG'CAT Cuts at: 4091 4091 Size: 7294 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2967 4399 5905 Size: 107 122 71 631 605 1295 1432 1506 Cuts at: 5905 136 Size: 1525 Fragments arranged by size: 1525 1506 1432 1295 631 605 122 107 71 BglII A'GATC_T Cuts at: 7289 7289 Size: 7294 BlpI GC'TnA_GC Cuts at: 4711 4711 Size: 7294 Bme1580I G_kGCm'C Cuts at: 1790 5355 6511 1790 Size: 3565 1156 2573 Fragments arranged by size: 3565 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7294 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2670 5025 5983 Size: 416 53 83 399 24 1253 2355 958 Cuts at: 5983 442 Size: 1753 Fragments arranged by size: 2355 1753 1253 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7294 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5954 1239 Size: 4715 2579 Bpu10I CC'TnA_GC Cuts at: 2080 3924 2080 Size: 1844 5450 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3142 4544 5419 6284 6582 3142 Size: 1402 875 865 298 3854 Fragments arranged by size: 3854 1402 875 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3072 3417 5957 882 Size: 617 1573 345 2540 2219 Fragments arranged by size: 2540 2219 1573 617 345 BsaAI yAC'GTr Cuts at: 493 1204 3243 4273 5085 493 Size: 711 2039 1030 812 2702 Fragments arranged by size: 2702 2039 1030 812 711 BsaBI GATnn'nnATC Cuts at: 7288 7288 Size: 7294 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5101 5483 6118 275 Size: 53 83 186 936 3568 382 635 1451 Fragments arranged by size: 3568 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2171 5727 6125 6399 6546 6957 2171 Size: 3556 398 274 147 411 2508 Fragments arranged by size: 3556 2508 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2584 2614 4185 4215 1178 Size: 30 165 30 1181 30 1571 30 4257 Fragments arranged by size: 4257 1571 1181 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2029 3785 4427 1186 Size: 843 1756 642 4053 Fragments arranged by size: 4053 1756 843 642 BseYI C'CCAG_C Cuts at: 2304 6497 6993 2304 Size: 4193 496 2605 Fragments arranged by size: 4193 2605 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2076 3688 1163 Size: 573 340 1612 4769 Fragments arranged by size: 4769 1612 573 340 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4744 5505 6533 1540 Size: 275 186 2743 761 1028 2301 Fragments arranged by size: 2743 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2021 4067 5355 5440 6511 2021 Size: 2046 1288 85 1071 2804 Fragments arranged by size: 2804 2046 1288 1071 85 BsmI GAATG_Cn' Cuts at: 2477 4091 4805 4898 2477 Size: 1614 714 93 4873 Fragments arranged by size: 4873 1614 714 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 4067 4613 5355 5440 6511 1391 Size: 399 231 2046 546 742 85 1071 2174 Fragments arranged by size: 2174 2046 1071 742 546 399 231 85 BspEI T'CCGG_A Cuts at: 2171 6957 2171 Size: 4786 2508 BspHI T'CATG_A Cuts at: 2896 4461 5183 6913 2896 Size: 1565 722 1730 3277 Fragments arranged by size: 3277 1730 1565 722 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2178 3817 4578 4649 844 Size: 1334 1639 761 71 3489 Fragments arranged by size: 3489 1639 1334 761 71 BsrBI CCG'CTC Cuts at: 1394 1823 2854 5181 1394 Size: 429 1031 2327 3507 Fragments arranged by size: 3507 2327 1031 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5789 5963 65 Size: 1183 4541 174 1396 Fragments arranged by size: 4541 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2614 4398 5938 6125 1700 Size: 110 804 1784 1540 187 2869 Fragments arranged by size: 2869 1784 1540 804 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7294 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7294 BssSI C'ACGA_G Cuts at: 2849 5354 6366 2849 Size: 2505 1012 3777 Fragments arranged by size: 3777 2505 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7294 BstBI TT'CG_AA Cuts at: 6098 6098 Size: 7294 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7294 BstXI CCAn_nnnn'nTGG Cuts at: 4330 4330 Size: 7294 BstYI r'GATC_y Cuts at: 1972 2174 4624 4991 5376 5393 6845 6931 6943 Size: 202 2450 367 385 17 1452 86 12 Cuts at: 6943 7289 1972 Size: 346 1977 Fragments arranged by size: 2450 1977 1452 385 367 346 202 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2299 2514 3082 3427 3586 513 Size: 552 939 295 215 568 345 159 4221 Fragments arranged by size: 4221 939 568 552 345 295 215 159 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6555 Fragments arranged by size: 6555 683 56 BtsI GCAGTG_nn' Cuts at: 2088 2359 2882 3819 4810 5604 5624 2088 Size: 271 523 937 991 794 20 3758 Fragments arranged by size: 3758 991 937 794 523 271 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7294 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7259 DraI TTT'AAA Cuts at: 4598 4598 Size: 7294 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6301 809 Size: 507 4985 1802 Fragments arranged by size: 4985 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2379 2655 4741 Size: 54 1425 50 275 18 549 276 2086 Cuts at: 4741 5630 7259 8 Size: 889 1629 43 Fragments arranged by size: 2086 1629 1425 889 549 276 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4741 1537 Size: 275 2929 4090 Fragments arranged by size: 4090 2929 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3777 4072 5224 1353 Size: 2424 295 1152 3423 Fragments arranged by size: 3423 2424 1152 295 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5881 6255 6401 387 Size: 1064 138 4292 374 146 1280 Fragments arranged by size: 4292 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2019 4065 2019 Size: 2046 5248 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3607 3665 5357 5954 6741 7009 1239 Size: 393 1975 58 1692 597 787 268 1524 Fragments arranged by size: 1975 1692 1524 787 597 393 268 58 EcoNI CCTnn'n_nnAGG Cuts at: 2608 2608 Size: 7294 EcoO109I rG'GnC_Cy Cuts at: 2129 3582 2129 Size: 1453 5841 EcoRI G'AATT_C Cuts at: 3180 4603 4981 3180 Size: 1423 378 5493 Fragments arranged by size: 5493 1423 378 EcoRV GAT'ATC Cuts at: 3334 3838 3334 Size: 504 6790 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3292 3393 3292 Size: 101 7193 FspI TGC'GCA Cuts at: 3611 5800 3611 Size: 2189 5105 HaeII r_GCGC'y Cuts at: 1678 6441 1678 Size: 4763 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2058 2090 2968 2971 3000 Size: 32 135 32 486 32 878 3 29 Cuts at: 3000 3003 3780 3812 3822 3854 3979 4011 4424 Size: 3 777 32 10 32 125 32 413 Cuts at: 4424 4456 5935 5967 6009 6041 1373 Size: 32 1479 32 42 32 2626 Fragments arranged by size: 2626 1479 878 777 486 413 135 125 42 32 32 32 32 32 32 32 32 32 29 10 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2777 3061 3448 4198 4638 4884 Size: 988 281 839 284 387 750 440 246 Cuts at: 4884 669 Size: 3079 Fragments arranged by size: 3079 988 839 750 440 387 284 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7294 HpaI GTT'AAC Cuts at: 4198 4884 4198 Size: 686 6608 KpnI G_GTAC'C Cuts at: 4619 4619 Size: 7294 MfeI C'AATT_G Cuts at: 4893 4893 Size: 7294 MluI A'CGCG_T Cuts at: 7170 7170 Size: 7294 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2044 3055 3720 4322 6408 6592 1305 Size: 739 1011 665 602 2086 184 2007 Fragments arranged by size: 2086 2007 1011 739 665 602 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2381 2657 10 Size: 54 1425 343 549 276 4647 Fragments arranged by size: 4647 1425 549 343 276 54 MslI CAynn'nnrTG Cuts at: 518 1166 2955 3054 4466 4578 5252 5611 5770 Size: 648 1789 99 1412 112 674 359 159 Cuts at: 5770 518 Size: 2042 Fragments arranged by size: 2042 1789 1412 674 648 359 159 112 99 MspA1I CmG'CkG Cuts at: 1606 4326 4710 5387 6535 6780 1606 Size: 2720 384 677 1148 245 2120 Fragments arranged by size: 2720 2120 1148 677 384 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3082 3586 513 Size: 552 939 1078 504 4221 Fragments arranged by size: 4221 1078 939 552 504 NdeI CA'TA_TG Cuts at: 387 4579 387 Size: 4192 3102 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7294 NotI GC'GGCC_GC Cuts at: 4741 4741 Size: 7294 NsiI A_TGCA'T Cuts at: 4093 4093 Size: 7294 NspI r_CATG'y Cuts at: 1132 1229 3105 3365 3457 4652 6197 1132 Size: 97 1876 260 92 1195 1545 2229 Fragments arranged by size: 2229 1876 1545 1195 260 97 92 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7294 PciI A'CATG_T Cuts at: 3101 6193 3101 Size: 3092 4202 PflMI CCAn_nnn'nTGG Cuts at: 1108 3088 3343 1108 Size: 1980 255 5059 Fragments arranged by size: 5059 1980 255 PmeI GTTT'AAAC Cuts at: 4598 4598 Size: 7294 PmlI CAC'GTG Cuts at: 3243 3243 Size: 7294 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5363 5395 6876 6908 5363 Size: 32 1481 32 5749 Fragments arranged by size: 5749 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2129 3582 2129 Size: 1453 5841 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7294 PsiI TTA'TAA Cuts at: 3390 4864 3390 Size: 1474 5820 PstI C_TGCA'G Cuts at: 830 2482 3814 4646 830 Size: 1652 1332 832 3478 Fragments arranged by size: 3478 1652 1332 832 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7294 PvuII CAG'CTG Cuts at: 4326 4326 Size: 7294 SacI G_AGCT'C Cuts at: 2021 4067 2021 Size: 2046 5248 SalI G'TCGA_C Cuts at: 1936 4636 1936 Size: 2700 4594 SanDI GG'GwC_CC Cuts at: 3582 3582 Size: 7294 SapI GCTCTTCn'nnn_ Cuts at: 4072 4072 Size: 7294 SbfI CC_TGCA'GG Cuts at: 2482 4646 2482 Size: 2164 5130 ScaI AGT'ACT Cuts at: 5542 7254 5542 Size: 1712 5582 SfcI C'TryA_G Cuts at: 826 1045 2136 2478 3777 3810 4309 4642 5777 Size: 219 1091 342 1299 33 499 333 1135 Cuts at: 5777 6458 6649 826 Size: 681 191 1471 Fragments arranged by size: 1471 1299 1135 1091 681 499 342 333 219 191 33 SfiI GGCCn_nnn'nGGCC Cuts at: 2967 2967 Size: 7294 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7294 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7294 SmaI CCC'GGG Cuts at: 1872 4621 1872 Size: 2749 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3121 4559 5398 6299 6561 820 Size: 197 926 1178 1438 839 901 262 1553 Fragments arranged by size: 1553 1438 1178 926 901 839 262 197 SnaBI TAC'GTA Cuts at: 493 5085 493 Size: 4592 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7294 SphI G_CATG'C Cuts at: 1132 3457 4652 1132 Size: 2325 1195 3774 Fragments arranged by size: 3774 2325 1195 SspI AAT'ATT Cuts at: 5 52 2569 2862 3879 5218 5 Size: 47 2517 293 1017 1339 2081 Fragments arranged by size: 2517 2081 1339 1017 293 47 StuI AGG'CCT Cuts at: 1517 2905 1517 Size: 1388 5906 StyI C'CwwG_G Cuts at: 513 1065 2004 3082 3586 4733 513 Size: 552 939 1078 504 1147 3074 Fragments arranged by size: 3074 1147 1078 939 552 504 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2899 5321 5338 5491 5676 6121 325 Size: 1255 1319 2422 17 153 185 445 1498 Fragments arranged by size: 2422 1498 1319 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2312 3492 5540 Size: 274 80 33 51 1443 335 1180 2048 Cuts at: 5540 7252 96 Size: 1712 138 Fragments arranged by size: 2048 1712 1443 1180 335 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2584 2616 4185 4217 7254 7286 2584 Size: 32 1569 32 3037 32 2592 Fragments arranged by size: 3037 2592 1569 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7294 XbaI T'CTAG_A Cuts at: 4630 4630 Size: 7294 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2407 2407 Size: 7294 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7294 XmaI C'CCGG_G Cuts at: 1870 4619 1870 Size: 2749 4545 XmnI GAAnn'nnTTC Cuts at: 4148 5423 4148 Size: 1275 6019 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5102 276 Size: 53 83 186 936 3568 2468 Fragments arranged by size: 3568 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI