(Linear) (Six-base) MAPSORT of: KIBB0450.seq Check: 5294 from: 1 to: 2589 KIBB0450.orf With 182 enzymes: SgfI * March 22, 2012 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 173 2589 Size: 173 2416 AccI GT'mk_AC Cuts at: 0 1771 2589 Size: 1771 818 AclI AA'CG_TT Cuts at: 0 1293 2589 Size: 1293 1296 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1602 1660 2589 Size: 1602 58 929 Fragments arranged by size: 1602 929 58 AflIII A'CryG_T Cuts at: 0 1096 1237 2589 Size: 1096 141 1352 Fragments arranged by size: 1352 1096 141 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 571 2589 Size: 571 2018 AlwNI CAG_nnn'CTG Cuts at: 0 257 2589 Size: 257 2332 ApoI r'AATT_y Cuts at: 0 745 1175 1186 2589 Size: 745 430 11 1403 Fragments arranged by size: 1403 745 430 11 AseI AT'TA_AT Cuts at: 0 496 1015 2589 Size: 496 519 1574 Fragments arranged by size: 1574 519 496 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 375 408 2589 Size: 375 33 2181 Fragments arranged by size: 2181 375 33 BanII G_rGCy'C Cuts at: 0 16 2062 2589 Size: 16 2046 527 Fragments arranged by size: 2046 527 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 292 2255 2589 Size: 292 1963 334 Fragments arranged by size: 1963 334 292 BbvCI CC'TCA_GC Cuts at: 0 1919 2589 Size: 1919 670 BciVI GTATCCnnnnn_n' Cuts at: 0 2126 2589 Size: 2126 463 BfrBI ATG'CAT Cuts at: 0 2086 2589 Size: 2086 503 BglI GCCn_nnn'nGGC Cuts at: 0 962 2394 2589 Size: 962 1432 195 Fragments arranged by size: 1432 962 195 BmrI ACTGGGnnnn_n' Cuts at: 0 665 2589 Size: 665 1924 Bpu10I CC'TnA_GC Cuts at: 0 75 1919 2589 Size: 75 1844 670 Fragments arranged by size: 1844 670 75 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1137 2539 2589 Size: 1137 1402 50 Fragments arranged by size: 1402 1137 50 BsaI GGTCTCn'nnnn_ Cuts at: 0 1067 1412 2589 Size: 1067 345 1177 Fragments arranged by size: 1177 1067 345 BsaAI yAC'GTr Cuts at: 0 1238 2268 2589 Size: 1238 1030 321 Fragments arranged by size: 1238 1030 321 BsaWI w'CCGG_w Cuts at: 0 166 2589 Size: 166 2423 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 579 609 2180 2210 2589 Size: 579 30 1571 30 379 Fragments arranged by size: 1571 579 379 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 24 1780 2422 2589 Size: 24 1756 642 167 Fragments arranged by size: 1756 642 167 24 BseYI C'CCAG_C Cuts at: 0 299 2589 Size: 299 2290 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 71 1683 2589 Size: 71 1612 906 Fragments arranged by size: 1612 906 71 BsiHKAI G_wGCw'C Cuts at: 0 16 2062 2589 Size: 16 2046 527 Fragments arranged by size: 2046 527 16 BsmI GAATG_Cn' Cuts at: 0 472 2086 2589 Size: 472 1614 503 Fragments arranged by size: 1614 503 472 Bsp1286I G_dGCh'C Cuts at: 0 16 2062 2589 Size: 16 2046 527 Fragments arranged by size: 2046 527 16 BspEI T'CCGG_A Cuts at: 0 166 2589 Size: 166 2423 BspHI T'CATG_A Cuts at: 0 891 2456 2589 Size: 891 1565 133 Fragments arranged by size: 1565 891 133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 173 1812 2573 2589 Size: 173 1639 761 16 Fragments arranged by size: 1639 761 173 16 BsrBI CCG'CTC Cuts at: 0 849 2589 Size: 849 1740 BsrFI r'CCGG_y Cuts at: 0 609 2393 2589 Size: 609 1784 196 Fragments arranged by size: 1784 609 196 BssSI C'ACGA_G Cuts at: 0 844 2589 Size: 844 1745 BstXI CCAn_nnnn'nTGG Cuts at: 0 2325 2589 Size: 2325 264 BstYI r'GATC_y Cuts at: 0 169 2589 Size: 169 2420 BtgI C'CryG_G Cuts at: 0 294 509 1077 1422 1581 2589 Size: 294 215 568 345 159 1008 Fragments arranged by size: 1008 568 345 294 215 159 BtsI GCAGTG_nn' Cuts at: 0 83 354 877 1814 2589 Size: 83 271 523 937 775 Fragments arranged by size: 937 775 523 271 83 EaeI y'GGCC_r Cuts at: 0 374 650 2589 Size: 374 276 1939 Fragments arranged by size: 1939 374 276 EarI CTCTTCn'nnn_ Cuts at: 0 1772 2067 2589 Size: 1772 295 522 Fragments arranged by size: 1772 522 295 EcoICRI GAG'CTC Cuts at: 0 14 2060 2589 Size: 14 2046 529 Fragments arranged by size: 2046 529 14 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1602 1660 2589 Size: 1602 58 929 Fragments arranged by size: 1602 929 58 EcoNI CCTnn'n_nnAGG Cuts at: 0 603 2589 Size: 603 1986 EcoO109I rG'GnC_Cy Cuts at: 0 124 1577 2589 Size: 124 1453 1012 Fragments arranged by size: 1453 1012 124 EcoRI G'AATT_C Cuts at: 0 1175 2589 Size: 1175 1414 EcoRV GAT'ATC Cuts at: 0 1329 1833 2589 Size: 1329 504 756 Fragments arranged by size: 1329 756 504 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1287 1388 2589 Size: 1287 101 1201 Fragments arranged by size: 1287 1201 101 FspI TGC'GCA Cuts at: 0 1606 2589 Size: 1606 983 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 53 85 963 966 995 998 1775 1807 Size: 53 32 878 3 29 3 777 32 Cuts at: 1807 1817 1849 1974 2006 2419 2451 2589 Size: 10 32 125 32 413 32 138 Fragments arranged by size: 878 777 413 138 125 53 32 32 32 32 32 29 10 3 3 HincII GTy'rAC Cuts at: 0 772 1056 1443 2193 2589 Size: 772 284 387 750 396 Fragments arranged by size: 772 750 396 387 284 HpaI GTT'AAC Cuts at: 0 2193 2589 Size: 2193 396 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 39 1050 1715 2317 2589 Size: 39 1011 665 602 272 Fragments arranged by size: 1011 665 602 272 39 MscI TGG'CCA Cuts at: 0 376 652 2589 Size: 376 276 1937 Fragments arranged by size: 1937 376 276 MslI CAynn'nnrTG Cuts at: 0 950 1049 2461 2573 2589 Size: 950 99 1412 112 16 Fragments arranged by size: 1412 950 112 99 16 MspA1I CmG'CkG Cuts at: 0 2321 2589 Size: 2321 268 NcoI C'CATG_G Cuts at: 0 1077 1581 2589 Size: 1077 504 1008 Fragments arranged by size: 1077 1008 504 NdeI CA'TA_TG Cuts at: 0 2574 2589 Size: 2574 15 NsiI A_TGCA'T Cuts at: 0 2088 2589 Size: 2088 501 NspI r_CATG'y Cuts at: 0 1100 1360 1452 2589 Size: 1100 260 92 1137 Fragments arranged by size: 1137 1100 260 92 PciI A'CATG_T Cuts at: 0 1096 2589 Size: 1096 1493 PflMI CCAn_nnn'nTGG Cuts at: 0 1083 1338 2589 Size: 1083 255 1251 Fragments arranged by size: 1251 1083 255 PmlI CAC'GTG Cuts at: 0 1238 2589 Size: 1238 1351 PpuMI rG'GwC_Cy Cuts at: 0 124 1577 2589 Size: 124 1453 1012 Fragments arranged by size: 1453 1012 124 PsiI TTA'TAA Cuts at: 0 1385 2589 Size: 1385 1204 PstI C_TGCA'G Cuts at: 0 477 1809 2589 Size: 477 1332 780 Fragments arranged by size: 1332 780 477 PvuII CAG'CTG Cuts at: 0 2321 2589 Size: 2321 268 SacI G_AGCT'C Cuts at: 0 16 2062 2589 Size: 16 2046 527 Fragments arranged by size: 2046 527 16 SanDI GG'GwC_CC Cuts at: 0 1577 2589 Size: 1577 1012 SapI GCTCTTCn'nnn_ Cuts at: 0 2067 2589 Size: 2067 522 SbfI CC_TGCA'GG Cuts at: 0 477 2589 Size: 477 2112 SfcI C'TryA_G Cuts at: 0 131 473 1772 1805 2304 2589 Size: 131 342 1299 33 499 285 Fragments arranged by size: 1299 499 342 285 131 33 SfiI GGCCn_nnn'nGGCC Cuts at: 0 962 2589 Size: 962 1627 SmlI C'TyrA_G Cuts at: 0 1116 2554 2589 Size: 1116 1438 35 Fragments arranged by size: 1438 1116 35 SphI G_CATG'C Cuts at: 0 1452 2589 Size: 1452 1137 SspI AAT'ATT Cuts at: 0 564 857 1874 2589 Size: 564 293 1017 715 Fragments arranged by size: 1017 715 564 293 StuI AGG'CCT Cuts at: 0 900 2589 Size: 900 1689 StyI C'CwwG_G Cuts at: 0 1077 1581 2589 Size: 1077 504 1008 Fragments arranged by size: 1077 1008 504 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 894 2589 Size: 894 1695 TatI w'GTAC_w Cuts at: 0 307 1487 2589 Size: 307 1180 1102 Fragments arranged by size: 1180 1102 307 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 579 611 2180 2212 2589 Size: 579 32 1569 32 377 Fragments arranged by size: 1569 579 377 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 402 2589 Size: 402 2187 XmnI GAAnn'nnTTC Cuts at: 0 2143 2589 Size: 2143 446 Enzymes that do cut: AarI AccI AclI AcuI AflIII AlfI AlwNI ApoI AseI BaeI BanII BbsI BbvCI BciVI BfrBI BglI BmrI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssSI BstXI BstYI BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI Hin4I HincII HpaI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PciI PflMI PmlI PpuMI PsiI PstI PvuII SacI SanDI SapI SbfI SfcI SfiI SmlI SphI SspI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BamHI BanI BbeI BcgI BclI BglII BlpI Bme1580I BmgBI BmtI BplI BpmI BsaBI BsaHI BsiEI BsiWI BsmBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI FseI FspAI HaeII HindIII KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PfoI PmeI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI XmaI ZraI