################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI AocI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MroI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuII RcaI RsaI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI TaqI Tth111I TthHB8I XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AcsI 817 GAATTC [GA]^AATT[CT] Alw44I 418 GTGCAC G^TGCAC AlwNI 239 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 747 AGCGCT AGC^GCT ApaI 788 GGGCCC GGGCC^C ApaLI 418 GTGCAC G^TGCAC ApoI 817 GAATTC [GA]^AATT[CT] AvaI 461 CCCGGG C^[CT]CG[GA]G AvrII 156 CCTAGG C^CTAGG BglII 546 AGATCT A^GATCT BlnI 156 CCTAGG C^CTAGG BpmI 86 CTCCAG CTGGAG(16/14) BsgI 106 CTGCAC GTGCAG(16/14) BsiEI 650 CGATCG CG[GA][CT]^CG BsoBI 461 CCCGGG C^[CT]CG[GA]G BsrFI 947 GCCGGC [GA]^CCGG[CT] BssSI 763 CTCGTG CACGAG(-5/-1) BstAPI 108 GCACAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 465 GGTCACC G^GT[ACGT]ACC BstPI 465 GGTCACC G^GT[ACGT]ACC BstXI 88 CCAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 546 AGATCT [GA]^GATC[CT] CfoI 748 GCGC GCG^C Cfr10I 947 GCCGGC [GA]^CCGG[CT] DraIII 109 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 747 AGCGCT AGC^GCT EcoO65I 465 GGTCACC G^GT[ACGT]ACC EcoRI 817 GAATTC G^AATTC HaeII 747 AGCGCT [GA]GCGC^[CT] HhaI 748 GCGC GCG^C HinP1I 748 GCGC G^CGC HinfI 921 GAATC G^A[ACGT]TC MaeII 209 ACGT A^CGT MfeI 883 CAATTG C^AATTG MflI 546 AGATCT [GA]^GATC[CT] MspA1I 445 CAGCGG C[AC]G^C[GT]G MunI 883 CAATTG C^AATTG NaeI 947 GCCGGC GCC^GGC NgoMIV 947 GCCGGC G^CCGGC NlaIII 583 CATG CATG^ PpuMI 98 GGGACCT [GA]G^G[AT]CC[CT] PshAI 960 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 650 CGATCG CGAT^CG SacI 25 GAGCTC GAGCT^C SmaI 461 CCCGGG CCC^GGG SmlI 501 CTTGAG C^T[CT][GA]AG SspI 619 AATATT AAT^ATT StuI 587 AGGCCT AGG^CCT TfiI 921 GAATC G^A[AT]TC TspRI 641 CACTG CA[GC]TG[ACGT][ACGT]^ XbaI 21 TCTAGA T^CTAGA XhoII 546 AGATCT [GA]^GATC[CT] XmaCI 461 CCCGGG C^CCGGG XmaI 461 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 409 CGCG CG^CG 692 CGCG AlwI 527 GATCC GGATC(4/5) 753 GATCC BbsI 181 GTCTTC GAAGAC(2/6) 655 GTCTTC BfaI 22 CTAG C^TAG 157 CTAG BglI 192 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 229 GCCAACCTGGC BpuAI 181 GTCTTC GAAGAC(2/6) 655 GTCTTC BsmFI 98 GGGAC GGGAC(10/14) 541 GTCCC BspMI 101 ACCTGC ACCTGC(4/8) 469 ACCTGC BstUI 409 CGCG CG^CG 692 CGCG EarI 52 CTCTTC CTCTTC(1/4) 952 CTCTTC EcoT14I 156 CCTAGG C^C[AT][AT]GG 962 CCAAGG FauI 693 GCGGG CCCGC(4/6) 719 GCGGG HphI 467 TCACC GGTGA(8/7) 557 TCACC Ksp632I 52 CTCTTC CTCTTC(1/4) 952 CTCTTC MaeI 22 CTAG C^TAG 157 CTAG MaeIII 77 GTGAC ^GT[ACGT]AC 466 GTCAC MseI 302 TTAA T^TAA 616 TTAA MslI 468 CACCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 759 CACGCTCGTG MvnI 409 CGCG CG^CG 692 CGCG PflMI 669 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 962 CCAAGGTCTGG SapI 51 GCTCTTC GCTCTTC(1/4) 951 GCTCTTC StyI 156 CCTAGG C^C[AT][AT]GG 962 CCAAGG Tru9I 302 TTAA T^TAA 616 TTAA Tsp45I 77 GTGAC ^GT[GC]AC 466 GTCAC Van91I 669 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 962 CCAAGGTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AluI AspHI AvaII BalI BanII BbvI BcnI BmyI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI MboI MboII MluNI MnlI MscI MspI MvaI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI Tsp509I