(Circular) (Six-base) MAPSORT of: pFN21AE9529.seq Check: 5353 from: 1 to: 5764 pFN21AE9529 5764 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2483 2483 Size: 5764 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3574 278 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3107 1937 Size: 1170 4594 Acc65I G'GTAC_C Cuts at: 3085 3085 Size: 5764 AclI AA'CG_TT Cuts at: 1563 3891 4264 1563 Size: 2328 373 3063 Fragments arranged by size: 3063 2328 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2944 3827 5211 5479 1632 Size: 1312 883 1384 268 1917 Fragments arranged by size: 1917 1384 1312 883 268 AfeI AGC'GCT Cuts at: 2754 2754 Size: 5764 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5567 AflIII A'CryG_T Cuts at: 1203 4663 5640 1203 Size: 3460 977 1327 Fragments arranged by size: 3460 1327 977 AgeI A'CCGG_T Cuts at: 4595 4595 Size: 5764 AhdI GACnn_n'nnGTC Cuts at: 4493 4493 Size: 5764 AleI CACnn'nnGTG Cuts at: 1166 2477 2768 1166 Size: 1311 291 4162 Fragments arranged by size: 4162 1311 291 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5764 AlwNI CAG_nnn'CTG Cuts at: 2249 3507 5079 2249 Size: 1258 1572 2934 Fragments arranged by size: 2934 1572 1258 ApaI G_GGCC'C Cuts at: 2797 2797 Size: 5764 ApaLI G'TGCA_C Cuts at: 2423 3821 4977 2423 Size: 1398 1156 3210 Fragments arranged by size: 3210 1398 1156 ApoI r'AATT_y Cuts at: 1493 2822 3073 3299 3451 5560 1493 Size: 1329 251 226 152 2109 1697 Fragments arranged by size: 2109 1697 1329 251 226 152 AseI AT'TA_AT Cuts at: 160 1032 4318 160 Size: 872 3286 1606 Fragments arranged by size: 3286 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 AvaI C'yCGr_G Cuts at: 1870 1943 2466 3089 1870 Size: 73 523 623 4545 Fragments arranged by size: 4545 623 523 73 AvrII C'CTAG_G Cuts at: 2161 2161 Size: 5764 BamHI G'GATC_C Cuts at: 3094 3461 3094 Size: 367 5397 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3085 4540 618 Size: 325 217 627 1298 1455 1842 Fragments arranged by size: 1842 1455 1298 627 325 217 BanII G_rGCy'C Cuts at: 1391 2034 2326 2797 3083 1391 Size: 643 292 471 286 4072 Fragments arranged by size: 4072 643 471 292 286 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2179 2653 928 Size: 971 280 474 4039 Fragments arranged by size: 4039 971 474 280 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3955 3989 1903 Size: 34 2018 34 3678 Fragments arranged by size: 3678 2018 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3656 4866 3656 Size: 1210 4554 BclI T'GATC_A Cuts at: 1548 1548 Size: 5764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2203 2240 4375 Size: 107 122 71 631 605 531 37 2135 Cuts at: 4375 136 Size: 1525 Fragments arranged by size: 2135 1525 631 605 531 122 107 71 37 BglII A'GATC_T Cuts at: 2551 5759 2551 Size: 3208 2556 BlpI GC'TnA_GC Cuts at: 3181 3181 Size: 5764 Bme1580I G_kGCm'C Cuts at: 1790 2427 2797 3825 4981 1790 Size: 637 370 1028 1156 2573 Fragments arranged by size: 2573 1156 1028 637 370 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5764 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3495 4453 442 Size: 416 53 83 399 24 2078 958 1753 Fragments arranged by size: 2078 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5764 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2345 2345 Size: 5764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2076 4424 1239 Size: 837 2348 2579 Fragments arranged by size: 2579 2348 837 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2527 3889 4754 5052 2527 Size: 1362 865 298 3239 Fragments arranged by size: 3239 1362 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4427 882 Size: 617 2928 2219 Fragments arranged by size: 2928 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3555 493 Size: 711 2351 2702 Fragments arranged by size: 2702 2351 711 BsaBI GATnn'nnATC Cuts at: 5758 5758 Size: 5764 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3571 3953 4588 275 Size: 53 83 186 936 2038 382 635 1451 Fragments arranged by size: 2038 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4197 4595 4869 5016 5427 4197 Size: 398 274 147 411 4534 Fragments arranged by size: 4534 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5539 Fragments arranged by size: 5539 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2318 2570 2717 1186 Size: 1132 252 147 4233 Fragments arranged by size: 4233 1132 252 147 BseYI C'CCAG_C Cuts at: 2790 4967 5463 2790 Size: 2177 496 3091 Fragments arranged by size: 3091 2177 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2096 1163 Size: 573 360 4831 Fragments arranged by size: 4831 573 360 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2658 3214 3975 5003 1540 Size: 275 186 657 556 761 1028 2301 Fragments arranged by size: 2301 1028 761 657 556 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 2427 2484 3825 3910 4981 2034 Size: 393 57 1341 85 1071 2817 Fragments arranged by size: 2817 1341 1071 393 85 57 BsmI GAATG_Cn' Cuts at: 3275 3368 3275 Size: 93 5671 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2326 2427 2484 2797 3083 3825 Size: 399 244 292 101 57 313 286 742 Cuts at: 3825 3910 4981 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 313 292 286 244 101 85 57 BspEI T'CCGG_A Cuts at: 5427 5427 Size: 5764 BspHI T'CATG_A Cuts at: 3653 5383 3653 Size: 1730 4034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2115 2483 3119 844 Size: 1271 368 636 3489 Fragments arranged by size: 3489 1271 636 368 BsrBI CCG'CTC Cuts at: 1394 1823 3651 1394 Size: 429 1828 3507 Fragments arranged by size: 3507 1828 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4259 4433 65 Size: 1183 3011 174 1396 Fragments arranged by size: 3011 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2952 4408 4595 1700 Size: 110 1142 1456 187 2869 Fragments arranged by size: 2869 1456 1142 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5764 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5764 BssSI C'ACGA_G Cuts at: 2768 3824 4836 2768 Size: 1056 1012 3696 Fragments arranged by size: 3696 1056 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2119 794 Size: 1325 4439 BstBI TT'CG_AA Cuts at: 4568 4568 Size: 5764 BstEII G'GTnAC_C Cuts at: 1184 2470 1184 Size: 1286 4478 BstXI CCAn_nnnn'nTGG Cuts at: 2100 2100 Size: 5764 BstYI r'GATC_y Cuts at: 1972 2551 3094 3461 3846 3863 5315 5401 5413 Size: 579 543 367 385 17 1452 86 12 Cuts at: 5413 5759 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 579 543 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4273 Fragments arranged by size: 4273 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5025 Fragments arranged by size: 5025 683 56 BtsI GCAGTG_nn' Cuts at: 3280 4074 4094 3280 Size: 794 20 4950 Fragments arranged by size: 4950 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2595 2630 529 Size: 35 2031 35 3663 Fragments arranged by size: 3663 2031 35 35 DraI TTT'AAA Cuts at: 3068 3068 Size: 5764 DraIII CAC_nnn'GTG Cuts at: 2119 2119 Size: 5764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4771 809 Size: 507 3455 1802 Fragments arranged by size: 3455 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 2391 2433 Size: 54 1425 50 275 18 402 159 42 Cuts at: 2433 2892 3211 4100 5729 8 Size: 459 319 889 1629 43 Fragments arranged by size: 1629 1425 889 459 402 319 275 159 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 3211 1537 Size: 275 1399 4090 Fragments arranged by size: 4090 1399 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2063 2963 3694 1353 Size: 710 900 731 3423 Fragments arranged by size: 3423 900 731 710 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2470 3073 4351 4725 4871 387 Size: 1064 138 881 603 1278 374 146 1280 Fragments arranged by size: 1280 1278 1064 881 603 374 146 138 EcoICRI GAG'CTC Cuts at: 2032 2032 Size: 5764 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2076 2944 3827 4424 5211 5479 1239 Size: 393 444 868 883 597 787 268 1524 Fragments arranged by size: 1524 883 868 787 597 444 393 268 EcoO109I rG'GnC_Cy Cuts at: 2104 2500 2794 2104 Size: 396 294 5074 Fragments arranged by size: 5074 396 294 EcoRI G'AATT_C Cuts at: 2822 3073 3451 2822 Size: 251 378 5135 Fragments arranged by size: 5135 378 251 FspI TGC'GCA Cuts at: 4270 4270 Size: 5764 HaeII r_GCGC'y Cuts at: 1678 2756 4911 1678 Size: 1078 2155 2531 Fragments arranged by size: 2531 2155 1078 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 4405 4437 4479 Size: 32 135 32 449 32 2352 32 42 Cuts at: 4479 4511 1373 Size: 32 2626 Fragments arranged by size: 2626 2352 449 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3108 3354 669 Size: 988 281 1170 246 3079 Fragments arranged by size: 3079 1170 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5764 HpaI GTT'AAC Cuts at: 3354 3354 Size: 5764 KpnI G_GTAC'C Cuts at: 3089 3089 Size: 5764 MfeI C'AATT_G Cuts at: 2888 3363 2888 Size: 475 5289 MluI A'CGCG_T Cuts at: 5640 5640 Size: 5764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2067 3070 4878 5062 1305 Size: 762 1003 1808 184 2007 Fragments arranged by size: 2007 1808 1003 762 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2234 2393 2435 2894 10 Size: 54 1425 343 402 159 42 459 2880 Fragments arranged by size: 2880 1425 459 402 343 159 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 2477 2768 3722 4081 4240 518 Size: 648 1311 291 954 359 159 2042 Fragments arranged by size: 2042 1311 954 648 359 291 159 MspA1I CmG'CkG Cuts at: 1606 2452 3180 3857 5005 5250 1606 Size: 846 728 677 1148 245 2120 Fragments arranged by size: 2120 1148 846 728 677 245 NaeI GCC'GGC Cuts at: 2954 2954 Size: 5764 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4273 Fragments arranged by size: 4273 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5764 NgoMIV G'CCGG_C Cuts at: 2952 2952 Size: 5764 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5764 NotI GC'GGCC_GC Cuts at: 3211 3211 Size: 5764 NspI r_CATG'y Cuts at: 1132 1229 3122 4667 1132 Size: 97 1893 1545 2229 Fragments arranged by size: 2229 1893 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5764 PciI A'CATG_T Cuts at: 4663 4663 Size: 5764 PflMI CCAn_nnn'nTGG Cuts at: 1108 2680 2973 1108 Size: 1572 293 3899 Fragments arranged by size: 3899 1572 293 PfoI T'CCnGG_A Cuts at: 2817 2817 Size: 5764 PmeI GTTT'AAAC Cuts at: 3068 3068 Size: 5764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3833 3865 5346 5378 3833 Size: 32 1481 32 4219 Fragments arranged by size: 4219 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2104 2104 Size: 5764 PshAI GACnn'nnGTC Cuts at: 1613 2969 1613 Size: 1356 4408 PsiI TTA'TAA Cuts at: 2985 3334 2985 Size: 349 5415 PspOMI G'GGCC_C Cuts at: 2793 2793 Size: 5764 PstI C_TGCA'G Cuts at: 830 3116 830 Size: 2286 3478 PvuI CG_AT'CG Cuts at: 2001 2658 2001 Size: 657 5107 SacI G_AGCT'C Cuts at: 2034 2034 Size: 5764 SalI G'TCGA_C Cuts at: 1936 3106 1936 Size: 1170 4594 SapI GCTCTTCn'nnn_ Cuts at: 2063 2963 2063 Size: 900 4864 SbfI CC_TGCA'GG Cuts at: 3116 3116 Size: 5764 ScaI AGT'ACT Cuts at: 4012 5724 4012 Size: 1712 4052 SfcI C'TryA_G Cuts at: 826 1045 3112 4247 4928 5119 826 Size: 219 2067 1135 681 191 1471 Fragments arranged by size: 2067 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5764 SmaI CCC'GGG Cuts at: 1872 2468 3091 1872 Size: 596 623 4545 Fragments arranged by size: 4545 623 596 SmlI C'TyrA_G Cuts at: 820 1017 1943 2506 3868 4769 5031 820 Size: 197 926 563 1362 901 262 1553 Fragments arranged by size: 1553 1362 926 901 563 262 197 SnaBI TAC'GTA Cuts at: 493 3555 493 Size: 3062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5764 SphI G_CATG'C Cuts at: 1132 3122 1132 Size: 1990 3774 SspI AAT'ATT Cuts at: 5 52 2626 3688 5 Size: 47 2574 1062 2081 Fragments arranged by size: 2574 2081 1062 47 StuI AGG'CCT Cuts at: 1517 2594 1517 Size: 1077 4687 StyI C'CwwG_G Cuts at: 513 1065 2004 2161 2967 3203 513 Size: 552 939 157 806 236 3074 Fragments arranged by size: 3074 939 806 552 236 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3791 3808 3961 4146 4591 325 Size: 1255 2211 17 153 185 445 1498 Fragments arranged by size: 2211 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4010 5722 96 Size: 274 80 33 51 1443 2033 1712 138 Fragments arranged by size: 2033 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5724 5756 5724 Size: 32 5732 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5764 XbaI T'CTAG_A Cuts at: 2026 3100 2026 Size: 1074 4690 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5764 XmaI C'CCGG_G Cuts at: 1870 2466 3089 1870 Size: 596 623 4545 Fragments arranged by size: 4545 623 596 XmnI GAAnn'nnTTC Cuts at: 3893 3893 Size: 5764 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3572 276 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI