(Circular) (Six-base) MAPSORT of: pFN21AE4582.seq Check: 9239 from: 1 to: 7156 pFN21AE4582 7156 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3361 3739 4027 3361 Size: 378 288 6490 Fragments arranged by size: 6490 378 288 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4966 278 Size: 53 83 186 936 3430 2468 Fragments arranged by size: 3430 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3459 4499 1937 Size: 1522 1040 4594 Fragments arranged by size: 4594 1522 1040 Acc65I G'GTAC_C Cuts at: 3881 4477 3881 Size: 596 6560 AclI AA'CG_TT Cuts at: 1563 4224 5283 5656 1563 Size: 2661 1059 373 3063 Fragments arranged by size: 3063 2661 1059 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5219 6603 6871 1632 Size: 3587 1384 268 1917 Fragments arranged by size: 3587 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6959 AflIII A'CryG_T Cuts at: 1203 6055 7032 1203 Size: 4852 977 1327 Fragments arranged by size: 4852 1327 977 AgeI A'CCGG_T Cuts at: 5987 5987 Size: 7156 AhdI GACnn_n'nnGTC Cuts at: 5885 5885 Size: 7156 AleI CACnn'nnGTG Cuts at: 1166 2543 3161 1166 Size: 1377 618 5161 Fragments arranged by size: 5161 1377 618 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2069 1184 Size: 885 6271 AlwNI CAG_nnn'CTG Cuts at: 2617 2693 3784 4042 4373 4400 4899 6471 2617 Size: 76 1091 258 331 27 499 1572 3302 Fragments arranged by size: 3302 1572 1091 499 331 258 76 27 ApaLI G'TGCA_C Cuts at: 4337 5213 6369 4337 Size: 876 1156 5124 Fragments arranged by size: 5124 1156 876 ApoI r'AATT_y Cuts at: 1493 2826 4465 4691 4843 6952 1493 Size: 1333 1639 226 152 2109 1697 Fragments arranged by size: 2109 1697 1639 1333 226 152 AseI AT'TA_AT Cuts at: 160 1032 5710 160 Size: 872 4678 1606 Fragments arranged by size: 4678 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7156 AvaI C'yCGr_G Cuts at: 1870 1943 2250 2280 2522 2970 3045 3352 4443 Size: 73 307 30 242 448 75 307 1091 Cuts at: 4443 4481 1870 Size: 38 4545 Fragments arranged by size: 4545 1091 448 307 307 242 75 73 38 30 BamHI G'GATC_C Cuts at: 4486 4853 4486 Size: 367 6789 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2312 2754 3050 3881 3919 Size: 325 217 627 525 442 296 831 38 Cuts at: 3919 4477 5932 618 Size: 558 1455 1842 Fragments arranged by size: 1842 1455 831 627 558 525 442 325 296 217 38 BanII G_rGCy'C Cuts at: 1391 2140 2258 2363 3728 4475 1391 Size: 749 118 105 1365 747 4072 Fragments arranged by size: 4072 1365 749 747 118 105 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2245 4132 4171 928 Size: 971 346 1887 39 3913 Fragments arranged by size: 3913 1887 971 346 39 BbvCI CC'TCA_GC Cuts at: 3492 3492 Size: 7156 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5347 5381 1903 Size: 34 3410 34 3678 Fragments arranged by size: 3678 3410 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2287 5048 6258 2287 Size: 2761 1210 3185 Fragments arranged by size: 3185 2761 1210 BclI T'GATC_A Cuts at: 1548 2547 3804 1548 Size: 999 1257 4900 Fragments arranged by size: 4900 1257 999 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5767 136 Size: 107 122 71 631 605 4095 1525 Fragments arranged by size: 4095 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2730 7151 2730 Size: 4421 2735 BlpI GC'TnA_GC Cuts at: 2357 3092 3727 4573 2357 Size: 735 635 846 4940 Fragments arranged by size: 4940 846 735 635 Bme1580I G_kGCm'C Cuts at: 1790 2540 2759 4341 5217 6373 1790 Size: 750 219 1582 876 1156 2573 Fragments arranged by size: 2573 1582 1156 876 750 219 BmgBI CAC'GTC Cuts at: 1318 4021 1318 Size: 2703 4453 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2194 2549 2951 Size: 416 53 83 399 24 777 355 402 Cuts at: 2951 4106 4887 5845 442 Size: 1155 781 958 1753 Fragments arranged by size: 1753 1155 958 781 777 416 402 399 355 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7156 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2061 2191 2325 3296 4422 5816 1239 Size: 822 130 134 971 1126 1394 2579 Fragments arranged by size: 2579 1394 1126 971 822 134 130 Bpu10I CC'TnA_GC Cuts at: 3492 3492 Size: 7156 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2519 5281 6146 6444 2519 Size: 2762 865 298 3231 Fragments arranged by size: 3231 2762 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2946 3796 3890 4418 5819 882 Size: 617 1447 850 94 528 1401 2219 Fragments arranged by size: 2219 1447 1401 850 617 528 94 BsaAI yAC'GTr Cuts at: 493 1204 2545 2776 4947 493 Size: 711 1341 231 2171 2702 Fragments arranged by size: 2702 2171 1341 711 231 BsaBI GATnn'nnATC Cuts at: 2735 3803 7150 2735 Size: 1068 3347 2741 Fragments arranged by size: 3347 2741 1068 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4963 5345 5980 275 Size: 53 83 186 936 3430 382 635 1451 Fragments arranged by size: 3430 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5589 5987 6261 6408 6819 5589 Size: 398 274 147 411 5926 Fragments arranged by size: 5926 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2059 2089 2690 2720 2943 Size: 30 165 30 656 30 601 30 223 Cuts at: 2943 2973 3194 3224 4092 4122 1178 Size: 30 221 30 868 30 4212 Fragments arranged by size: 4212 868 656 601 223 221 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2130 2133 2444 2447 3575 1186 Size: 944 3 311 3 1128 4767 Fragments arranged by size: 4767 1128 944 311 3 3 BseYI C'CCAG_C Cuts at: 2693 3135 4366 6359 6855 2693 Size: 442 1231 1993 496 2994 Fragments arranged by size: 2994 1993 1231 496 442 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3824 1163 Size: 573 2088 4495 Fragments arranged by size: 4495 2088 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4606 5367 6395 1540 Size: 275 186 2605 761 1028 2301 Fragments arranged by size: 2605 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2363 2391 3282 4341 5217 5302 6373 2363 Size: 28 891 1059 876 85 1071 3146 Fragments arranged by size: 3146 1071 1059 891 876 85 28 BsmI GAATG_Cn' Cuts at: 4667 4760 4667 Size: 93 7063 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 2258 2363 2391 2540 2759 3282 Size: 399 350 118 105 28 149 219 523 Cuts at: 3282 3728 4341 4475 5217 5302 6373 1391 Size: 446 613 134 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 613 523 446 399 350 219 149 134 118 105 85 28 BspEI T'CCGG_A Cuts at: 6819 6819 Size: 7156 BspHI T'CATG_A Cuts at: 5045 6775 5045 Size: 1730 5426 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2631 3361 3739 4027 4511 844 Size: 1787 730 378 288 484 3489 Fragments arranged by size: 3489 1787 730 484 378 288 BsrBI CCG'CTC Cuts at: 1394 1823 2860 3664 4032 5043 1394 Size: 429 1037 804 368 1011 3507 Fragments arranged by size: 3507 1037 1011 804 429 368 BsrDI GCAATG_nn' Cuts at: 65 1248 2731 4249 4302 5651 5825 65 Size: 1183 1483 1518 53 1349 174 1396 Fragments arranged by size: 1518 1483 1396 1349 1183 174 53 BsrFI r'CCGG_y Cuts at: 1700 1810 2268 2922 3969 5800 5987 1700 Size: 110 458 654 1047 1831 187 2869 Fragments arranged by size: 2869 1831 1047 654 458 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7156 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7156 BssSI C'ACGA_G Cuts at: 5216 6228 5216 Size: 1012 6144 BstAPI GCAn_nnn'nTGC Cuts at: 794 3414 794 Size: 2620 4536 BstBI TT'CG_AA Cuts at: 5960 5960 Size: 7156 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7156 BstXI CCAn_nnnn'nTGG Cuts at: 3066 3751 3952 4435 3066 Size: 685 201 483 5787 Fragments arranged by size: 5787 685 483 201 BstYI r'GATC_y Cuts at: 1972 2730 3084 3343 3781 4215 4486 4853 5238 Size: 758 354 259 438 434 271 367 385 Cuts at: 5238 5255 6707 6793 6805 7151 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 758 438 434 385 367 354 346 271 259 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2431 2887 2947 2985 3240 3691 513 Size: 552 1366 456 60 38 255 451 3978 Fragments arranged by size: 3978 1366 552 456 451 255 60 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6417 Fragments arranged by size: 6417 683 56 BtsI GCAGTG_nn' Cuts at: 2578 3409 4113 4256 4672 5466 5486 2578 Size: 831 704 143 416 794 20 4248 Fragments arranged by size: 4248 831 794 704 416 143 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7156 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2587 2622 529 Size: 35 2023 35 5063 Fragments arranged by size: 5063 2023 35 35 DraI TTT'AAA Cuts at: 4460 4460 Size: 7156 DraIII CAC_nnn'GTG Cuts at: 2893 2893 Size: 7156 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3011 6163 809 Size: 507 1695 3152 1802 Fragments arranged by size: 3152 1802 1695 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2875 3127 3183 Size: 54 1425 50 275 18 1045 252 56 Cuts at: 3183 3741 4044 4114 4603 5492 7121 8 Size: 558 303 70 489 889 1629 43 Fragments arranged by size: 1629 1425 1045 889 558 489 303 275 252 70 56 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4603 1537 Size: 275 2791 4090 Fragments arranged by size: 4090 2791 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2747 3329 5086 1353 Size: 1394 582 1757 3423 Fragments arranged by size: 3423 1757 1394 582 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3202 5743 6117 6263 387 Size: 1064 138 1613 2541 374 146 1280 Fragments arranged by size: 2541 1613 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2361 2361 Size: 7156 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 2191 2325 3296 4422 5219 5816 Size: 393 429 130 134 971 1126 797 597 Cuts at: 5816 6603 6871 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1126 971 797 787 597 429 393 268 134 130 EcoO109I rG'GnC_Cy Cuts at: 2189 3927 4289 2189 Size: 1738 362 5056 Fragments arranged by size: 5056 1738 362 EcoRI G'AATT_C Cuts at: 4465 4843 4465 Size: 378 6778 FspI TGC'GCA Cuts at: 4070 5662 4070 Size: 1592 5564 HaeII r_GCGC'y Cuts at: 1678 6303 1678 Size: 4625 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 2943 2975 3102 Size: 32 135 32 518 32 821 32 127 Cuts at: 3102 3134 3328 3335 3360 3367 4413 4445 5797 Size: 32 194 7 25 7 1046 32 1352 Cuts at: 5797 5829 5871 5903 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1352 1046 821 518 194 135 127 42 32 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 4500 4746 669 Size: 988 281 2562 246 3079 Fragments arranged by size: 3079 2562 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7156 HpaI GTT'AAC Cuts at: 4746 4746 Size: 7156 KpnI G_GTAC'C Cuts at: 3885 4481 3885 Size: 596 6560 MfeI C'AATT_G Cuts at: 4755 4755 Size: 7156 MluI A'CGCG_T Cuts at: 7032 7032 Size: 7156 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3630 6270 6454 1305 Size: 2325 2640 184 2007 Fragments arranged by size: 2640 2325 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3129 3185 3743 4046 4116 Size: 54 1425 343 1297 56 558 303 70 Cuts at: 4116 10 Size: 3050 Fragments arranged by size: 3050 1425 1297 558 343 303 70 56 54 MslI CAynn'nnrTG Cuts at: 518 1166 2543 2555 3064 3161 3749 3950 4352 Size: 648 1377 12 509 97 588 201 402 Cuts at: 4352 4433 5114 5473 5632 518 Size: 81 681 359 159 2042 Fragments arranged by size: 2042 1377 681 648 588 509 402 359 201 159 97 81 12 MspA1I CmG'CkG Cuts at: 1606 2289 2303 2433 3523 3731 4572 5249 6397 Size: 683 14 130 1090 208 841 677 1148 Cuts at: 6397 6642 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1090 841 683 677 245 208 130 14 NaeI GCC'GGC Cuts at: 2270 2270 Size: 7156 NcoI C'CATG_G Cuts at: 513 1065 2985 3240 513 Size: 552 1920 255 4429 Fragments arranged by size: 4429 1920 552 255 NdeI CA'TA_TG Cuts at: 387 2448 2938 387 Size: 2061 490 4605 Fragments arranged by size: 4605 2061 490 NgoMIV G'CCGG_C Cuts at: 2268 2268 Size: 7156 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7156 NotI GC'GGCC_GC Cuts at: 4603 4603 Size: 7156 NspI r_CATG'y Cuts at: 1132 1229 2332 3613 4514 6059 1132 Size: 97 1103 1281 901 1545 2229 Fragments arranged by size: 2229 1545 1281 1103 901 97 PasI CC'CwG_GG Cuts at: 1348 4373 1348 Size: 3025 4131 PciI A'CATG_T Cuts at: 6055 6055 Size: 7156 PflMI CCAn_nnn'nTGG Cuts at: 1108 3982 4373 1108 Size: 2874 391 3891 Fragments arranged by size: 3891 2874 391 PfoI T'CCnGG_A Cuts at: 2465 2916 2465 Size: 451 6705 PmeI GTTT'AAAC Cuts at: 4460 4460 Size: 7156 PmlI CAC'GTG Cuts at: 2545 2545 Size: 7156 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5225 5257 6738 6770 5225 Size: 32 1481 32 5611 Fragments arranged by size: 5611 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2189 2189 Size: 7156 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7156 PsiI TTA'TAA Cuts at: 4726 4726 Size: 7156 PstI C_TGCA'G Cuts at: 830 4508 830 Size: 3678 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7156 PvuII CAG'CTG Cuts at: 2303 2303 Size: 7156 RsrII CG'GwC_CG Cuts at: 3934 3934 Size: 7156 SacI G_AGCT'C Cuts at: 2363 2363 Size: 7156 SacII CC_GC'GG Cuts at: 2434 2434 Size: 7156 SalI G'TCGA_C Cuts at: 1936 4498 1936 Size: 2562 4594 SbfI CC_TGCA'GG Cuts at: 4508 4508 Size: 7156 ScaI AGT'ACT Cuts at: 5404 7116 5404 Size: 1712 5444 SfcI C'TryA_G Cuts at: 826 1045 4504 5639 6320 6511 826 Size: 219 3459 1135 681 191 1471 Fragments arranged by size: 3459 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7156 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7156 SmaI CCC'GGG Cuts at: 1872 2252 2282 2972 4483 1872 Size: 380 30 690 1511 4545 Fragments arranged by size: 4545 1511 690 380 30 SmlI C'TyrA_G Cuts at: 820 1017 1943 2498 4443 5260 6161 6423 820 Size: 197 926 555 1945 817 901 262 1553 Fragments arranged by size: 1945 1553 926 901 817 555 262 197 SnaBI TAC'GTA Cuts at: 493 4947 493 Size: 4454 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7156 SphI G_CATG'C Cuts at: 1132 2332 4514 1132 Size: 1200 2182 3774 Fragments arranged by size: 3774 2182 1200 SspI AAT'ATT Cuts at: 5 52 5080 5 Size: 47 5028 2081 Fragments arranged by size: 5028 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7156 StyI C'CwwG_G Cuts at: 513 1065 2985 3240 4595 513 Size: 552 1920 255 1355 3074 Fragments arranged by size: 3074 1920 1355 552 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2855 3058 3354 3657 5183 5200 5353 Size: 1255 1275 203 296 303 1526 17 153 Cuts at: 5353 5538 5983 325 Size: 185 445 1498 Fragments arranged by size: 1526 1498 1275 1255 445 303 296 203 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3513 4129 5402 Size: 274 80 33 51 1443 1536 616 1273 Cuts at: 5402 7114 96 Size: 1712 138 Fragments arranged by size: 1712 1536 1443 1273 616 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2059 2091 4092 4124 7116 7148 2059 Size: 32 2001 32 2992 32 2067 Fragments arranged by size: 2992 2067 2001 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2957 1316 Size: 1641 5515 XbaI T'CTAG_A Cuts at: 4492 4492 Size: 7156 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3907 3907 Size: 7156 XhoI C'TCGA_G Cuts at: 1943 4443 1943 Size: 2500 4656 XmaI C'CCGG_G Cuts at: 1870 2250 2280 2970 4481 1870 Size: 380 30 690 1511 4545 Fragments arranged by size: 4545 1511 690 380 30 XmnI GAAnn'nnTTC Cuts at: 5285 5285 Size: 7156 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4964 276 Size: 53 83 186 936 3430 2468 Fragments arranged by size: 3430 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI SanDI SapI SexAI SfiI SfoI SrfI SwaI