(Linear) (Six-base) MAPSORT of: KIEE4582.seq Check: 7966 from: 1 to: 2451 KIEE4582.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1356 1734 2022 2451 Size: 1356 378 288 429 Fragments arranged by size: 1356 429 378 288 AccI GT'mk_AC Cuts at: 0 1454 2451 Size: 1454 997 Acc65I G'GTAC_C Cuts at: 0 1876 2451 Size: 1876 575 AclI AA'CG_TT Cuts at: 0 2219 2451 Size: 2219 232 AleI CACnn'nnGTG Cuts at: 0 538 1156 2451 Size: 538 618 1295 Fragments arranged by size: 1295 618 538 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 64 2451 Size: 64 2387 AlwNI CAG_nnn'CTG Cuts at: 0 612 688 1779 2037 2368 2395 2451 Size: 612 76 1091 258 331 27 56 Fragments arranged by size: 1091 612 331 258 76 56 27 ApaLI G'TGCA_C Cuts at: 0 2332 2451 Size: 2332 119 ApoI r'AATT_y Cuts at: 0 821 2451 Size: 821 1630 AvaI C'yCGr_G Cuts at: 0 245 275 517 965 1040 1347 2438 2451 Size: 245 30 242 448 75 307 1091 13 Fragments arranged by size: 1091 448 307 245 242 75 30 13 BanI G'GyrC_C Cuts at: 0 307 749 1045 1876 1914 2451 Size: 307 442 296 831 38 537 Fragments arranged by size: 831 537 442 307 296 38 BanII G_rGCy'C Cuts at: 0 135 253 358 1723 2451 Size: 135 118 105 1365 728 Fragments arranged by size: 1365 728 135 118 105 BbsI GAAGACnn'nnnn_ Cuts at: 0 240 2127 2166 2451 Size: 240 1887 39 285 Fragments arranged by size: 1887 285 240 39 BbvCI CC'TCA_GC Cuts at: 0 1487 2451 Size: 1487 964 BciVI GTATCCnnnnn_n' Cuts at: 0 282 2451 Size: 282 2169 BclI T'GATC_A Cuts at: 0 542 1799 2451 Size: 542 1257 652 Fragments arranged by size: 1257 652 542 BglII A'GATC_T Cuts at: 0 725 2451 Size: 725 1726 BlpI GC'TnA_GC Cuts at: 0 352 1087 1722 2451 Size: 352 735 635 729 Fragments arranged by size: 735 729 635 352 Bme1580I G_kGCm'C Cuts at: 0 535 754 2336 2451 Size: 535 219 1582 115 Fragments arranged by size: 1582 535 219 115 BmgBI CAC'GTC Cuts at: 0 2016 2451 Size: 2016 435 BmrI ACTGGGnnnn_n' Cuts at: 0 189 544 946 2101 2451 Size: 189 355 402 1155 350 Fragments arranged by size: 1155 402 355 350 189 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 56 186 320 1291 2417 2451 Size: 56 130 134 971 1126 34 Fragments arranged by size: 1126 971 134 130 56 34 Bpu10I CC'TnA_GC Cuts at: 0 1487 2451 Size: 1487 964 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 514 2451 Size: 514 1937 BsaI GGTCTCn'nnnn_ Cuts at: 0 941 1791 1885 2413 2451 Size: 941 850 94 528 38 Fragments arranged by size: 941 850 528 94 38 BsaAI yAC'GTr Cuts at: 0 540 771 2451 Size: 540 231 1680 Fragments arranged by size: 1680 540 231 BsaBI GATnn'nnATC Cuts at: 0 730 1798 2451 Size: 730 1068 653 Fragments arranged by size: 1068 730 653 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 54 84 685 715 938 968 1189 1219 Size: 54 30 601 30 223 30 221 30 Cuts at: 1219 2087 2117 2451 Size: 868 30 334 Fragments arranged by size: 868 601 334 223 221 54 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 125 128 439 442 1570 2451 Size: 125 3 311 3 1128 881 Fragments arranged by size: 1128 881 311 125 3 3 BseYI C'CCAG_C Cuts at: 0 688 1130 2361 2451 Size: 688 442 1231 90 Fragments arranged by size: 1231 688 442 90 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1819 2451 Size: 1819 632 BsiHKAI G_wGCw'C Cuts at: 0 358 386 1277 2336 2451 Size: 358 28 891 1059 115 Fragments arranged by size: 1059 891 358 115 28 Bsp1286I G_dGCh'C Cuts at: 0 135 253 358 386 535 754 1277 1723 Size: 135 118 105 28 149 219 523 446 Cuts at: 1723 2336 2451 Size: 613 115 Fragments arranged by size: 613 523 446 219 149 135 118 115 105 28 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 626 1356 1734 2022 2451 Size: 626 730 378 288 429 Fragments arranged by size: 730 626 429 378 288 BsrBI CCG'CTC Cuts at: 0 855 1659 2027 2451 Size: 855 804 368 424 Fragments arranged by size: 855 804 424 368 BsrDI GCAATG_nn' Cuts at: 0 726 2244 2297 2451 Size: 726 1518 53 154 Fragments arranged by size: 1518 726 154 53 BsrFI r'CCGG_y Cuts at: 0 263 917 1964 2451 Size: 263 654 1047 487 Fragments arranged by size: 1047 654 487 263 BstAPI GCAn_nnn'nTGC Cuts at: 0 1409 2451 Size: 1409 1042 BstXI CCAn_nnnn'nTGG Cuts at: 0 1061 1746 1947 2430 2451 Size: 1061 685 201 483 21 Fragments arranged by size: 1061 685 483 201 21 BstYI r'GATC_y Cuts at: 0 725 1079 1338 1776 2210 2451 Size: 725 354 259 438 434 241 Fragments arranged by size: 725 438 434 354 259 241 BtgI C'CryG_G Cuts at: 0 426 882 942 980 1235 1686 2451 Size: 426 456 60 38 255 451 765 Fragments arranged by size: 765 456 451 426 255 60 38 BtsI GCAGTG_nn' Cuts at: 0 573 1404 2108 2251 2451 Size: 573 831 704 143 200 Fragments arranged by size: 831 704 573 200 143 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 582 617 2451 Size: 582 35 1834 Fragments arranged by size: 1834 582 35 DraIII CAC_nnn'GTG Cuts at: 0 888 2451 Size: 888 1563 DrdI GACnn_nn'nnGTC Cuts at: 0 1006 2451 Size: 1006 1445 EaeI y'GGCC_r Cuts at: 0 870 1122 1178 1736 2039 2109 2451 Size: 870 252 56 558 303 70 342 Fragments arranged by size: 870 558 342 303 252 70 56 EarI CTCTTCn'nnn_ Cuts at: 0 742 1324 2451 Size: 742 582 1127 Fragments arranged by size: 1127 742 582 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1197 2451 Size: 1197 1254 EcoICRI GAG'CTC Cuts at: 0 356 2451 Size: 356 2095 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 56 186 320 1291 2417 2451 Size: 56 130 134 971 1126 34 Fragments arranged by size: 1126 971 134 130 56 34 EcoO109I rG'GnC_Cy Cuts at: 0 184 1922 2284 2451 Size: 184 1738 362 167 Fragments arranged by size: 1738 362 184 167 FspI TGC'GCA Cuts at: 0 2065 2451 Size: 2065 386 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 85 117 938 970 1097 1129 1323 1330 Size: 85 32 821 32 127 32 194 7 Cuts at: 1330 1355 1362 2408 2440 2451 Size: 25 7 1046 32 11 Fragments arranged by size: 1046 821 194 127 85 32 32 32 32 25 11 7 7 KpnI G_GTAC'C Cuts at: 0 1880 2451 Size: 1880 571 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1625 2451 Size: 1625 826 MscI TGG'CCA Cuts at: 0 1124 1180 1738 2041 2111 2451 Size: 1124 56 558 303 70 340 Fragments arranged by size: 1124 558 340 303 70 56 MslI CAynn'nnrTG Cuts at: 0 538 550 1059 1156 1744 1945 2347 2428 Size: 538 12 509 97 588 201 402 81 Cuts at: 2428 2451 Size: 23 Fragments arranged by size: 588 538 509 402 201 97 81 23 12 MspA1I CmG'CkG Cuts at: 0 284 298 428 1518 1726 2451 Size: 284 14 130 1090 208 725 Fragments arranged by size: 1090 725 284 208 130 14 NaeI GCC'GGC Cuts at: 0 265 2451 Size: 265 2186 NcoI C'CATG_G Cuts at: 0 980 1235 2451 Size: 980 255 1216 Fragments arranged by size: 1216 980 255 NdeI CA'TA_TG Cuts at: 0 443 933 2451 Size: 443 490 1518 Fragments arranged by size: 1518 490 443 NgoMIV G'CCGG_C Cuts at: 0 263 2451 Size: 263 2188 NspI r_CATG'y Cuts at: 0 327 1608 2451 Size: 327 1281 843 Fragments arranged by size: 1281 843 327 PasI CC'CwG_GG Cuts at: 0 2368 2451 Size: 2368 83 PflMI CCAn_nnn'nTGG Cuts at: 0 1977 2368 2451 Size: 1977 391 83 Fragments arranged by size: 1977 391 83 PfoI T'CCnGG_A Cuts at: 0 460 911 2451 Size: 460 451 1540 Fragments arranged by size: 1540 460 451 PmlI CAC'GTG Cuts at: 0 540 2451 Size: 540 1911 PpuMI rG'GwC_Cy Cuts at: 0 184 2451 Size: 184 2267 PvuII CAG'CTG Cuts at: 0 298 2451 Size: 298 2153 RsrII CG'GwC_CG Cuts at: 0 1929 2451 Size: 1929 522 SacI G_AGCT'C Cuts at: 0 358 2451 Size: 358 2093 SacII CC_GC'GG Cuts at: 0 429 2451 Size: 429 2022 SmaI CCC'GGG Cuts at: 0 247 277 967 2451 Size: 247 30 690 1484 Fragments arranged by size: 1484 690 247 30 SmlI C'TyrA_G Cuts at: 0 493 2438 2451 Size: 493 1945 13 Fragments arranged by size: 1945 493 13 SphI G_CATG'C Cuts at: 0 327 2451 Size: 327 2124 StyI C'CwwG_G Cuts at: 0 980 1235 2451 Size: 980 255 1216 Fragments arranged by size: 1216 980 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 850 1053 1349 1652 2451 Size: 850 203 296 303 799 Fragments arranged by size: 850 799 303 296 203 TatI w'GTAC_w Cuts at: 0 1508 2124 2451 Size: 1508 616 327 Fragments arranged by size: 1508 616 327 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 54 86 2087 2119 2451 Size: 54 32 2001 32 332 Fragments arranged by size: 2001 332 54 32 32 Tth111I GACn'n_nGTC Cuts at: 0 952 2451 Size: 952 1499 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1902 2451 Size: 1902 549 XhoI C'TCGA_G Cuts at: 0 2438 2451 Size: 2438 13 XmaI C'CCGG_G Cuts at: 0 245 275 965 2451 Size: 245 30 690 1486 Fragments arranged by size: 1486 690 245 30 Enzymes that do cut: AarI AccI Acc65I AclI AleI AlfI AlwNI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FspI Hin4I KpnI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PvuII RsrII SacI SacII SmaI SmlI SphI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AatII AcuI AfeI AflII AflIII AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BfrBI BglI BmtI BplI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI EagI EcoNI EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI NarI NheI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI XbaI XmnI ZraI