################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BspHI BspLU11I BspMI BsrDI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccII 1390 CGCG CG^CG AccIII 1375 TCCGGA T^CCGGA AfaI 1114 GTAC GT^AC AlwI 1317 GATCC GGATC(4/5) AlwNI 451 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 180 GAGCTC G[AT]GC[AT]^C AvaI 927 CCCGGG C^[CT]CG[GA]G BbsI 1728 GTCTTC GAAGAC(2/6) BcgI 1254 GCAACAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1666 GGATAC GTATCC(6/5) BpuAI 1728 GTCTTC GAAGAC(2/6) BsaWI 1375 TCCGGA [AT]^CCGG[AT] BseAI 1375 TCCGGA T^CCGGA BsgI 1204 GTGCAG GTGCAG(16/14) BsiHKAI 180 GAGCTC G[AT]GC[AT]^C BsmAI 1104 GAGAC GTCTC(1/5) BsmBI 1104 GAGACG CGTCTC(1/5) BsmI 918 GAATGC GAATGC(1/-1) BsoBI 927 CCCGGG C^[CT]CG[GA]G Bsp1407I 1113 TGTACA T^GTACA BspDI 607 ATCGAT AT^CGAT BspEI 1375 TCCGGA T^CCGGA BsrBI 1235 CCGCTC CCGCTC(-3/-3) BsrGI 1113 TGTACA T^GTACA BstBI 881 TTCGAA TT^CGAA BstUI 1390 CGCG CG^CG BstYI 1316 AGATCC [GA]^GATC[CT] CfoI 732 GCGC GCG^C ClaI 607 ATCGAT AT^CGAT EcoNI 789 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 41 CCCGC CCCGC(4/6) HgaI 1388 GACGC GACGC(5/10) HhaI 732 GCGC GCG^C HinP1I 732 GCGC G^CGC HphI 898 GGTGA GGTGA(8/7) MaeII 1107 ACGT A^CGT MflI 1316 AGATCC [GA]^GATC[CT] MroI 1375 TCCGGA T^CCGGA MvnI 1390 CGCG CG^CG NspV 881 TTCGAA TT^CGAA PflMI 735 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 689 CAGCTG CAG^CTG RsaI 1114 GTAC GT^AC SacI 180 GAGCTC GAGCT^C SapI 832 GAAGAGC GCTCTTC(1/4) SfuI 881 TTCGAA TT^CGAA SmaI 927 CCCGGG CCC^GGG SmlI 18 CTCAAG C^T[CT][GA]AG SspBI 1113 TGTACA T^GTACA Tsp45I 581 GTGAC ^GT[GC]AC Van91I 735 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 794 CCAAGGCTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1316 AGATCC [GA]^GATC[CT] XmaCI 927 CCCGGG C^CCGGG XmaI 927 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AcsI 104 GAATTT [GA]^AATT[CT] 1334 AAATTC ApoI 104 GAATTT [GA]^AATT[CT] 1334 AAATTC AvaII 1715 GGTCC G^G[AT]CC 1741 GGACC BfaI 110 CTAG C^TAG 1357 CTAG BsmFI 1740 GGGAC GGGAC(10/14) 1140 GTCCC BssSI 859 CACGAG CACGAG(-5/-1) 578 CTCGTG EaeI 1467 CGGCCA [CT]^GGCC[GA] 1560 CGGCCA EcoRV 1071 GATATC GAT^ATC 1230 GATATC MaeI 110 CTAG C^TAG 1357 CTAG MaeIII 442 GTAAC ^GT[ACGT]AC 581 GTGAC MseI 617 TTAA T^TAA 1313 TTAA PleI 942 GACTC GAGTC(4/5) 1726 GAGTC SfaNI 810 GATGC GCATC(5/9) 1003 GATGC Tru9I 617 TTAA T^TAA 1313 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AluI BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfcI StyI TaqI TfiI TseI Tsp509I TspRI TthHB8I