(Circular) (Six-base) MAPSORT of: pFN21AB6122.seq Check: 9285 from: 1 to: 6457 pFN21AB6122 6457 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4267 278 Size: 53 83 186 936 2731 2468 Fragments arranged by size: 2731 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3800 1937 Size: 1863 4594 Acc65I G'GTAC_C Cuts at: 3778 3778 Size: 6457 AclI AA'CG_TT Cuts at: 1563 4584 4957 1563 Size: 3021 373 3063 Fragments arranged by size: 3063 3021 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2654 2689 2732 2856 2873 3033 4520 5904 Size: 1022 35 43 124 17 160 1487 1384 Cuts at: 5904 6172 1632 Size: 268 1917 Fragments arranged by size: 1917 1487 1384 1022 268 160 124 43 35 17 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6260 AflIII A'CryG_T Cuts at: 1203 5356 6333 1203 Size: 4153 977 1327 Fragments arranged by size: 4153 1327 977 AgeI A'CCGG_T Cuts at: 5288 5288 Size: 6457 AhdI GACnn_n'nnGTC Cuts at: 5186 5186 Size: 6457 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6457 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6457 AlwNI CAG_nnn'CTG Cuts at: 2461 4200 5772 2461 Size: 1739 1572 3146 Fragments arranged by size: 3146 1739 1572 ApaLI G'TGCA_C Cuts at: 4514 5670 4514 Size: 1156 5301 ApoI r'AATT_y Cuts at: 1493 2109 3339 3766 3992 4144 6253 1493 Size: 616 1230 427 226 152 2109 1697 Fragments arranged by size: 2109 1697 1230 616 427 226 152 AseI AT'TA_AT Cuts at: 160 1032 5011 160 Size: 872 3979 1606 Fragments arranged by size: 3979 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6457 AvaI C'yCGr_G Cuts at: 1870 1943 2932 3782 1870 Size: 73 989 850 4545 Fragments arranged by size: 4545 989 850 73 BamHI G'GATC_C Cuts at: 3787 4154 3787 Size: 367 6090 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3778 5233 618 Size: 325 217 627 1991 1455 1842 Fragments arranged by size: 1991 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2189 2213 2503 3776 1391 Size: 798 24 290 1273 4072 Fragments arranged by size: 4072 1273 798 290 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3726 928 Size: 971 1827 3659 Fragments arranged by size: 3659 1827 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3248 3282 4648 4682 1903 Size: 34 1311 34 1366 34 3678 Fragments arranged by size: 3678 1366 1311 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3665 4349 5559 3665 Size: 684 1210 4563 Fragments arranged by size: 4563 1210 684 BclI T'GATC_A Cuts at: 1548 1548 Size: 6457 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2690 3182 3480 Size: 107 122 71 631 605 1018 492 298 Cuts at: 3480 3573 5068 136 Size: 93 1495 1525 Fragments arranged by size: 1525 1495 1018 631 605 492 298 122 107 93 71 BglII A'GATC_T Cuts at: 6452 6452 Size: 6457 BlpI GC'TnA_GC Cuts at: 3874 3874 Size: 6457 Bme1580I G_kGCm'C Cuts at: 1790 4518 5674 1790 Size: 2728 1156 2573 Fragments arranged by size: 2728 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6457 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4188 5146 442 Size: 416 53 83 399 24 2771 958 1753 Fragments arranged by size: 2771 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6457 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2831 3149 3350 3541 3673 5117 1239 Size: 1592 318 201 191 132 1444 2579 Fragments arranged by size: 2579 1592 1444 318 201 191 132 Bpu10I CC'TnA_GC Cuts at: 2149 2149 Size: 6457 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2008 4582 5447 5745 2008 Size: 2574 865 298 2720 Fragments arranged by size: 2720 2574 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5120 882 Size: 617 3621 2219 Fragments arranged by size: 3621 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4248 493 Size: 711 3044 2702 Fragments arranged by size: 3044 2702 711 BsaBI GATnn'nnATC Cuts at: 6451 6451 Size: 6457 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4264 4646 5281 275 Size: 53 83 186 936 2731 382 635 1451 Fragments arranged by size: 2731 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3380 4890 5288 5562 5709 6120 3380 Size: 1510 398 274 147 411 3717 Fragments arranged by size: 3717 1510 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 2079 2109 2176 Size: 30 165 30 624 30 22 30 67 Cuts at: 2176 2206 3692 3722 1178 Size: 30 1486 30 3913 Fragments arranged by size: 3913 1486 624 165 67 30 30 30 30 30 30 22 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2070 2073 2076 2079 2082 2085 2097 2100 Size: 884 3 3 3 3 3 12 3 Cuts at: 2100 2103 2106 2403 2427 2882 3700 1186 Size: 3 3 297 24 455 818 3943 Fragments arranged by size: 3943 884 818 455 297 24 12 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 5660 6156 5660 Size: 496 5961 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3230 1163 Size: 573 1494 4390 Fragments arranged by size: 4390 1494 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3907 4668 5696 1540 Size: 275 186 1906 761 1028 2301 Fragments arranged by size: 2301 1906 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2189 4518 4603 5674 2189 Size: 2329 85 1071 2972 Fragments arranged by size: 2972 2329 1071 85 BsmI GAATG_Cn' Cuts at: 2929 3968 4061 2929 Size: 1039 93 5325 Fragments arranged by size: 5325 1039 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3103 3103 Size: 6457 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2189 2213 2503 3776 4518 4603 5674 Size: 399 399 24 290 1273 742 85 1071 Cuts at: 5674 1391 Size: 2174 Fragments arranged by size: 2174 1273 1071 742 399 399 290 85 24 BspEI T'CCGG_A Cuts at: 3380 6120 3380 Size: 2740 3717 BspHI T'CATG_A Cuts at: 4346 6076 4346 Size: 1730 4727 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3812 844 Size: 2968 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3242 4344 1394 Size: 429 1419 1102 3507 Fragments arranged by size: 3507 1419 1102 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4952 5126 65 Size: 1183 3704 174 1396 Fragments arranged by size: 3704 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5101 5288 1700 Size: 110 3291 187 2869 Fragments arranged by size: 3291 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3118 96 Size: 3022 3435 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6457 BssSI C'ACGA_G Cuts at: 2583 2864 4517 5529 2583 Size: 281 1653 1012 3511 Fragments arranged by size: 3511 1653 1012 281 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6457 BstBI TT'CG_AA Cuts at: 2887 5261 2887 Size: 2374 4083 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6457 BstYI r'GATC_y Cuts at: 1972 3321 3787 4154 4539 4556 6008 6094 6106 Size: 1349 466 367 385 17 1452 86 12 Cuts at: 6106 6452 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1349 466 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2179 3475 3568 3723 513 Size: 552 1114 1296 93 155 3247 Fragments arranged by size: 3247 1296 1114 552 155 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5718 Fragments arranged by size: 5718 683 56 BtsI GCAGTG_nn' Cuts at: 2056 3973 4767 4787 2056 Size: 1917 794 20 3726 Fragments arranged by size: 3726 1917 794 20 ClaI AT'CG_AT Cuts at: 1554 2613 1554 Size: 1059 5398 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6422 DraI TTT'AAA Cuts at: 3761 3761 Size: 6457 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5464 809 Size: 507 4148 1802 Fragments arranged by size: 4148 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3472 3565 3904 Size: 54 1425 50 275 18 1642 93 339 Cuts at: 3904 4793 6422 8 Size: 889 1629 43 Fragments arranged by size: 1642 1629 1425 889 339 275 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3904 1537 Size: 275 2092 4090 Fragments arranged by size: 4090 2092 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2044 2832 3718 4387 1353 Size: 691 788 886 669 3423 Fragments arranged by size: 3423 886 788 691 669 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3405 3417 3444 3459 3522 3537 Size: 1064 138 1816 12 27 15 63 15 Cuts at: 3537 3564 3684 5044 5418 5564 387 Size: 27 120 1360 374 146 1280 Fragments arranged by size: 1816 1360 1280 1064 374 146 138 120 63 27 27 15 15 12 EcoICRI GAG'CTC Cuts at: 2187 2187 Size: 6457 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2654 2689 2732 2831 2856 2873 3033 Size: 393 1022 35 43 99 25 17 160 Cuts at: 3033 3149 3350 3541 3673 4520 5117 5904 6172 Size: 116 201 191 132 847 597 787 268 Cuts at: 6172 1239 Size: 1524 Fragments arranged by size: 1524 1022 847 787 597 393 268 201 191 160 132 116 99 43 35 25 17 EcoNI CCTnn'n_nnAGG Cuts at: 2798 2798 Size: 6457 EcoRI G'AATT_C Cuts at: 3766 4144 3766 Size: 378 6079 EcoRV GAT'ATC Cuts at: 3078 3237 3078 Size: 159 6298 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2147 2147 Size: 6457 FspI TGC'GCA Cuts at: 4963 4963 Size: 6457 HaeII r_GCGC'y Cuts at: 1678 5604 1678 Size: 3926 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3226 3258 3722 3754 5098 Size: 32 135 32 1654 32 464 32 1344 Cuts at: 5098 5130 5172 5204 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1654 1344 464 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3801 4047 669 Size: 988 281 1863 246 3079 Fragments arranged by size: 3079 1863 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6457 HpaI GTT'AAC Cuts at: 4047 4047 Size: 6457 KpnI G_GTAC'C Cuts at: 3782 3782 Size: 6457 MfeI C'AATT_G Cuts at: 4056 4056 Size: 6457 MluI A'CGCG_T Cuts at: 6333 6333 Size: 6457 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3349 5571 5755 1305 Size: 2044 2222 184 2007 Fragments arranged by size: 2222 2044 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4635 Fragments arranged by size: 4635 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4415 4774 4933 518 Size: 648 3249 359 159 2042 Fragments arranged by size: 3249 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2696 3561 3585 3693 3873 4550 5698 5943 Size: 1090 865 24 108 180 677 1148 245 Cuts at: 5943 1606 Size: 2120 Fragments arranged by size: 2120 1148 1090 865 677 245 180 108 24 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5905 NdeI CA'TA_TG Cuts at: 387 387 Size: 6457 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6457 NotI GC'GGCC_GC Cuts at: 3904 3904 Size: 6457 NspI r_CATG'y Cuts at: 1132 1229 3815 5360 1132 Size: 97 2586 1545 2229 Fragments arranged by size: 2586 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6457 PciI A'CATG_T Cuts at: 5356 5356 Size: 6457 PflMI CCAn_nnn'nTGG Cuts at: 1108 2746 1108 Size: 1638 4819 PfoI T'CCnGG_A Cuts at: 3309 3309 Size: 6457 PmeI GTTT'AAAC Cuts at: 3761 3761 Size: 6457 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4526 4558 6039 6071 4526 Size: 32 1481 32 4912 Fragments arranged by size: 4912 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6457 PsiI TTA'TAA Cuts at: 4027 4027 Size: 6457 PstI C_TGCA'G Cuts at: 830 3809 830 Size: 2979 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6457 PvuII CAG'CTG Cuts at: 2696 2696 Size: 6457 SacI G_AGCT'C Cuts at: 2189 2189 Size: 6457 SalI G'TCGA_C Cuts at: 1936 3799 1936 Size: 1863 4594 SapI GCTCTTCn'nnn_ Cuts at: 2832 2832 Size: 6457 SbfI CC_TGCA'GG Cuts at: 3809 3809 Size: 6457 ScaI AGT'ACT Cuts at: 4705 6417 4705 Size: 1712 4745 SfcI C'TryA_G Cuts at: 826 1045 2590 3048 3140 3805 4940 5621 5812 Size: 219 1545 458 92 665 1135 681 191 Cuts at: 5812 826 Size: 1471 Fragments arranged by size: 1545 1471 1135 681 665 458 219 191 92 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6457 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6457 SmaI CCC'GGG Cuts at: 1872 2934 3784 1872 Size: 1062 850 4545 Fragments arranged by size: 4545 1062 850 SmlI C'TyrA_G Cuts at: 820 1017 1943 2023 4561 5462 5724 820 Size: 197 926 80 2538 901 262 1553 Fragments arranged by size: 2538 1553 926 901 262 197 80 SnaBI TAC'GTA Cuts at: 493 4248 493 Size: 3755 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6457 SphI G_CATG'C Cuts at: 1132 3815 1132 Size: 2683 3774 SspI AAT'ATT Cuts at: 5 52 4381 5 Size: 47 4329 2081 Fragments arranged by size: 4329 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6457 StyI C'CwwG_G Cuts at: 513 1065 2131 2799 3180 3896 513 Size: 552 1066 668 381 716 3074 Fragments arranged by size: 3074 1066 716 668 552 381 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4484 4501 4654 4839 5284 325 Size: 1255 2904 17 153 185 445 1498 Fragments arranged by size: 2904 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3118 4703 6415 Size: 274 80 33 51 1443 1141 1585 1712 Cuts at: 6415 96 Size: 138 Fragments arranged by size: 1712 1585 1443 1141 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2024 2056 2079 2111 2176 2208 3692 3724 6417 Size: 32 23 32 65 32 1484 32 2693 Cuts at: 6417 6449 2024 Size: 32 2032 Fragments arranged by size: 2693 2032 1484 65 32 32 32 32 32 23 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6457 XbaI T'CTAG_A Cuts at: 3793 3793 Size: 6457 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2806 2806 Size: 6457 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6457 XmaI C'CCGG_G Cuts at: 1870 2932 3782 1870 Size: 1062 850 4545 Fragments arranged by size: 4545 1062 850 XmnI GAAnn'nnTTC Cuts at: 4586 4586 Size: 6457 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4265 276 Size: 53 83 186 936 2731 2468 Fragments arranged by size: 2731 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI