Restriction map for
pFN21AB6122
(Linear) (Six-base) MAPSORT of: KIBB6122.seq Check: 2560 from: 1 to: 1752
KIBB6122.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 684 727 851 868 1028 1752
Size: 649 35 43 124 17 160 724
Fragments arranged by size:
724 649 160 124 43 35 17
AlwNI CAG_nnn'CTG
Cuts at: 0 456 1752
Size: 456 1296
ApoI r'AATT_y
Cuts at: 0 104 1334 1752
Size: 104 1230 418
Fragments arranged by size:
1230 418 104
AvaI C'yCGr_G
Cuts at: 0 927 1752
Size: 927 825
BanII G_rGCy'C
Cuts at: 0 184 208 498 1752
Size: 184 24 290 1254
Fragments arranged by size:
1254 290 184 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 1721 1752
Size: 1721 31
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1243 1277 1752
Size: 1243 34 475
Fragments arranged by size:
1243 475 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1660 1752
Size: 1660 92
BglI GCCn_nnn'nGGC
Cuts at: 0 685 1177 1475 1568 1752
Size: 685 492 298 93 184
Fragments arranged by size:
685 492 298 184 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 1144 1345 1536 1668 1752
Size: 826 318 201 191 132 84
Fragments arranged by size:
826 318 201 191 132 84
Bpu10I CC'TnA_GC
Cuts at: 0 144 1752
Size: 144 1608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 1752
Size: 3 1749
BsaWI w'CCGG_w
Cuts at: 0 1375 1752
Size: 1375 377
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 74 104 171 201 1687 1717
Size: 22 30 22 30 67 30 1486 30
Cuts at: 1717 1752
Size: 35
Fragments arranged by size:
1486 67 35 30 30 30 30 22
22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 65 68 71 74 77 80 92 95
Size: 65 3 3 3 3 3 12 3
Cuts at: 95 98 101 398 422 877 1695 1752
Size: 3 3 297 24 455 818 57
Fragments arranged by size:
818 455 297 65 57 24 12 3
3 3 3 3 3 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1225 1752
Size: 1225 527
BsiHKAI G_wGCw'C
Cuts at: 0 184 1752
Size: 184 1568
BsmI GAATG_Cn'
Cuts at: 0 924 1752
Size: 924 828
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1098 1752
Size: 1098 654
Bsp1286I G_dGCh'C
Cuts at: 0 184 208 498 1752
Size: 184 24 290 1254
Fragments arranged by size:
1254 290 184 24
BspEI T'CCGG_A
Cuts at: 0 1375 1752
Size: 1375 377
BsrBI CCG'CTC
Cuts at: 0 1237 1752
Size: 1237 515
BsrGI T'GTAC_A
Cuts at: 0 1113 1752
Size: 1113 639
BssSI C'ACGA_G
Cuts at: 0 578 859 1752
Size: 578 281 893
Fragments arranged by size:
893 578 281
BstBI TT'CG_AA
Cuts at: 0 882 1752
Size: 882 870
BstYI r'GATC_y
Cuts at: 0 1316 1752
Size: 1316 436
BtgI C'CryG_G
Cuts at: 0 174 1470 1563 1718 1752
Size: 174 1296 93 155 34
Fragments arranged by size:
1296 174 155 93 34
BtsI GCAGTG_nn'
Cuts at: 0 51 1752
Size: 51 1701
ClaI AT'CG_AT
Cuts at: 0 608 1752
Size: 608 1144
EaeI y'GGCC_r
Cuts at: 0 1467 1560 1752
Size: 1467 93 192
Fragments arranged by size:
1467 192 93
EarI CTCTTCn'nnn_
Cuts at: 0 39 827 1713 1752
Size: 39 788 886 39
Fragments arranged by size:
886 788 39 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1400 1412 1439 1454 1517 1532 1559 1679
Size: 1400 12 27 15 63 15 27 120
Cuts at: 1679 1752
Size: 73
Fragments arranged by size:
1400 120 73 63 27 27 15 15
12
EcoICRI GAG'CTC
Cuts at: 0 182 1752
Size: 182 1570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 684 727 826 851 868 1028 1144
Size: 649 35 43 99 25 17 160 116
Cuts at: 1144 1345 1536 1668 1752
Size: 201 191 132 84
Fragments arranged by size:
649 201 191 160 132 116 99 84
43 35 25 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 793 1752
Size: 793 959
EcoRV GAT'ATC
Cuts at: 0 1073 1232 1752
Size: 1073 159 520
Fragments arranged by size:
1073 520 159
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 142 1752
Size: 142 1610
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1221 1253 1717 1749 1752
Size: 1221 32 464 32 3
Fragments arranged by size:
1221 464 32 32 3
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1344 1752
Size: 1344 408
MspA1I CmG'CkG
Cuts at: 0 691 1556 1580 1688 1752
Size: 691 865 24 108 64
Fragments arranged by size:
865 691 108 64 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 741 1752
Size: 741 1011
PfoI T'CCnGG_A
Cuts at: 0 1304 1752
Size: 1304 448
PvuII CAG'CTG
Cuts at: 0 691 1752
Size: 691 1061
SacI G_AGCT'C
Cuts at: 0 184 1752
Size: 184 1568
SapI GCTCTTCn'nnn_
Cuts at: 0 827 1752
Size: 827 925
SfcI C'TryA_G
Cuts at: 0 585 1043 1135 1752
Size: 585 458 92 617
Fragments arranged by size:
617 585 458 92
SmaI CCC'GGG
Cuts at: 0 929 1752
Size: 929 823
SmlI C'TyrA_G
Cuts at: 0 18 1752
Size: 18 1734
StyI C'CwwG_G
Cuts at: 0 126 794 1175 1752
Size: 126 668 381 577
Fragments arranged by size:
668 577 381 126
TatI w'GTAC_w
Cuts at: 0 1113 1752
Size: 1113 639
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 19 51 74 106 171 203 1687 1719
Size: 19 32 23 32 65 32 1484 32
Cuts at: 1719 1752
Size: 33
Fragments arranged by size:
1484 65 33 32 32 32 32 23
19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 801 1752
Size: 801 951
XmaI C'CCGG_G
Cuts at: 0 927 1752
Size: 927 825
Enzymes that do cut:
AcuI AlwNI ApoI AvaI BanII BbsI BcgI BciVI
BglI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrBI BsrGI BssSI
BstBI BstYI BtgI BtsI ClaI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoRV FalI Hin4I MmeI MspA1I
PflMI PfoI PvuII SacI SapI SfcI SmaI SmlI
StyI TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BsaI BsaAI BsaBI BsaHI BseYI BsiEI BsiWI
BspHI BspMI BsrDI BsrFI BssHII BstAPI BstEII BstXI
BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI
EcoO109I EcoRI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XhoI XmnI ZraI