(Circular) (Six-base) MAPSORT of: pFN21AE3608.seq Check: 7852 from: 1 to: 8701 pFN21AE3608 8701 bp With 182 enzymes: SgfI * March 28, 2012 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3180 3180 Size: 8701 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4617 6511 278 Size: 53 83 186 936 3081 1894 2468 Fragments arranged by size: 3081 2468 1894 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 5505 5580 5709 6044 1937 Size: 1113 2455 75 129 335 4594 Fragments arranged by size: 4594 2455 1113 335 129 75 Acc65I G'GTAC_C Cuts at: 6022 6022 Size: 8701 AclI AA'CG_TT Cuts at: 1563 6828 7201 1563 Size: 5265 373 3063 Fragments arranged by size: 5265 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2399 2876 3452 3791 3960 4701 6764 8148 Size: 767 477 576 339 169 741 2063 1384 Cuts at: 8148 8416 1632 Size: 268 1917 Fragments arranged by size: 2063 1917 1384 767 741 576 477 339 268 169 AflII C'TTAA_G Cuts at: 820 1017 2909 820 Size: 197 1892 6612 Fragments arranged by size: 6612 1892 197 AflIII A'CryG_T Cuts at: 1203 2807 5718 7600 8577 1203 Size: 1604 2911 1882 977 1327 Fragments arranged by size: 2911 1882 1604 1327 977 AgeI A'CCGG_T Cuts at: 7532 7532 Size: 8701 AhdI GACnn_n'nnGTC Cuts at: 4474 4816 7430 4474 Size: 342 2614 5745 Fragments arranged by size: 5745 2614 342 AleI CACnn'nnGTG Cuts at: 1166 4601 4661 5361 1166 Size: 3435 60 700 4506 Fragments arranged by size: 4506 3435 700 60 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2172 3438 5696 1184 Size: 988 1266 2258 4189 Fragments arranged by size: 4189 2258 1266 988 AlwNI CAG_nnn'CTG Cuts at: 2345 2854 3127 3310 3493 3788 3808 6444 8016 Size: 509 273 183 183 295 20 2636 1572 Cuts at: 8016 2345 Size: 3030 Fragments arranged by size: 3030 2636 1572 509 295 273 183 183 20 ApaI G_GGCC'C Cuts at: 3565 3678 4167 5017 5437 3565 Size: 113 489 850 420 6829 Fragments arranged by size: 6829 850 489 420 113 ApaLI G'TGCA_C Cuts at: 5615 6758 7914 5615 Size: 1143 1156 6402 Fragments arranged by size: 6402 1156 1143 ApoI r'AATT_y Cuts at: 1493 3922 5453 6010 6236 6388 8497 1493 Size: 2429 1531 557 226 152 2109 1697 Fragments arranged by size: 2429 2109 1697 1531 557 226 152 AseI AT'TA_AT Cuts at: 160 1032 7255 160 Size: 872 6223 1606 Fragments arranged by size: 6223 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8701 AvaI C'yCGr_G Cuts at: 1870 1943 2287 3152 3564 3900 5010 5584 6026 Size: 73 344 865 412 336 1110 574 442 Cuts at: 6026 1870 Size: 4545 Fragments arranged by size: 4545 1110 865 574 442 412 344 336 73 BamHI G'GATC_C Cuts at: 6031 6398 6031 Size: 367 8334 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3219 4006 4145 4272 4655 Size: 325 217 627 1432 787 139 127 383 Cuts at: 4655 6022 7477 618 Size: 1367 1455 1842 Fragments arranged by size: 1842 1455 1432 1367 787 627 383 325 217 139 127 BanII G_rGCy'C Cuts at: 1391 2285 2695 2728 3565 3651 3678 3726 4167 Size: 894 410 33 837 86 27 48 441 Cuts at: 4167 4853 5017 5211 5414 5437 5884 6020 1391 Size: 686 164 194 203 23 447 136 4072 Fragments arranged by size: 4072 894 837 686 447 441 410 203 194 164 136 86 48 33 27 23 BbeI G_GCGC'C Cuts at: 3223 3223 Size: 8701 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5857 928 Size: 971 3958 3772 Fragments arranged by size: 3958 3772 971 BbvCI CC'TCA_GC Cuts at: 2499 2499 Size: 8701 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4930 4964 6892 6926 1903 Size: 34 2993 34 1928 34 3678 Fragments arranged by size: 3678 2993 1928 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4573 6593 7803 4573 Size: 2020 1210 5471 Fragments arranged by size: 5471 2020 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8701 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4162 4396 7312 Size: 107 122 71 631 605 2490 234 2916 Cuts at: 7312 136 Size: 1525 Fragments arranged by size: 2916 2490 1525 631 605 234 122 107 71 BglII A'GATC_T Cuts at: 8696 8696 Size: 8701 BlpI GC'TnA_GC Cuts at: 2532 4173 6118 2532 Size: 1641 1945 5115 Fragments arranged by size: 5115 1945 1641 Bme1580I G_kGCm'C Cuts at: 1790 3565 3678 3901 4148 4167 4275 4943 5017 Size: 1775 113 223 247 19 108 668 74 Cuts at: 5017 5111 5358 5437 5619 6762 7918 1790 Size: 94 247 79 182 1143 1156 2573 Fragments arranged by size: 2573 1775 1156 1143 668 247 247 223 182 113 108 94 79 74 19 BmgBI CAC'GTC Cuts at: 1318 4738 4969 1318 Size: 3420 231 5050 Fragments arranged by size: 5050 3420 231 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3796 3888 6007 Size: 416 53 83 399 24 2379 92 2119 Cuts at: 6007 6432 7390 442 Size: 425 958 1753 Fragments arranged by size: 2379 2119 1753 958 425 416 399 92 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8701 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3040 5851 3040 Size: 2811 5890 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2577 3062 3063 3314 3383 3392 4143 4316 Size: 1338 485 1 251 69 9 751 173 Cuts at: 4316 4911 5222 5536 7361 1239 Size: 595 311 314 1825 2579 Fragments arranged by size: 2579 1825 1338 751 595 485 314 311 251 173 69 9 1 Bpu10I CC'TnA_GC Cuts at: 2499 2828 3645 2499 Size: 329 817 7555 Fragments arranged by size: 7555 817 329 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2747 3416 5390 5519 5876 6826 7691 7989 2747 Size: 669 1974 129 357 950 865 298 3459 Fragments arranged by size: 3459 1974 950 865 669 357 298 129 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2661 3141 5018 7364 882 Size: 617 1162 480 1877 2346 2219 Fragments arranged by size: 2346 2219 1877 1162 617 480 BsaAI yAC'GTr Cuts at: 493 1204 5721 6492 493 Size: 711 4517 771 2702 Fragments arranged by size: 4517 2702 771 711 BsaBI GATnn'nnATC Cuts at: 8695 8695 Size: 8701 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3220 4614 6508 6890 Size: 53 83 186 936 1687 1394 1894 382 Cuts at: 6890 7525 275 Size: 635 1451 Fragments arranged by size: 1894 1687 1451 1394 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5037 5274 7134 7532 7806 7953 8364 5037 Size: 237 1860 398 274 147 411 5374 Fragments arranged by size: 5374 1860 411 398 274 237 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5774 5804 1178 Size: 30 165 30 4371 30 4075 Fragments arranged by size: 4371 4075 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2204 2969 2999 3215 3227 3245 3335 3338 Size: 1018 765 30 216 12 18 90 3 Cuts at: 3338 3389 3690 3918 4234 4309 4502 4529 4634 Size: 51 301 228 316 75 193 27 105 Cuts at: 4634 4700 5534 1186 Size: 66 834 4353 Fragments arranged by size: 4353 1018 834 765 316 301 228 216 193 105 90 75 66 51 30 27 18 12 3 BseYI C'CCAG_C Cuts at: 2014 2069 3844 3953 3967 4233 4635 4748 5808 Size: 55 1775 109 14 266 402 113 1060 Cuts at: 5808 7904 8400 2014 Size: 2096 496 2315 Fragments arranged by size: 2315 2096 1775 1060 496 402 266 113 109 55 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3161 5097 5273 1163 Size: 573 1425 1936 176 4591 Fragments arranged by size: 4591 1936 1425 573 176 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6151 6912 7940 1540 Size: 275 186 4150 761 1028 2301 Fragments arranged by size: 4150 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2533 4347 4853 5414 5619 5884 6762 6847 7918 Size: 1814 506 561 205 265 878 85 1071 Cuts at: 7918 2533 Size: 3316 Fragments arranged by size: 3316 1814 1071 878 561 506 265 205 85 BsiWI C'GTAC_G Cuts at: 5721 5721 Size: 8701 BsmI GAATG_Cn' Cuts at: 2500 6212 6305 2500 Size: 3712 93 4896 Fragments arranged by size: 4896 3712 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3401 5837 3401 Size: 2436 6265 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2285 2533 2695 2728 3565 3651 3678 Size: 399 495 248 162 33 837 86 27 Cuts at: 3678 3726 3901 4148 4167 4275 4347 4853 4943 Size: 48 175 247 19 108 72 506 90 Cuts at: 4943 5017 5111 5211 5358 5414 5437 5619 5884 Size: 74 94 100 147 56 23 182 265 Cuts at: 5884 6020 6762 6847 7918 1391 Size: 136 742 85 1071 2174 Fragments arranged by size: 2174 1071 837 742 506 495 399 265 248 247 182 175 162 147 136 108 100 94 90 86 85 74 72 56 48 33 27 23 19 BspEI T'CCGG_A Cuts at: 5037 5274 8364 5037 Size: 237 3090 5374 Fragments arranged by size: 5374 3090 237 BspHI T'CATG_A Cuts at: 3030 5967 5991 6590 8320 3030 Size: 2937 24 599 1730 3411 Fragments arranged by size: 3411 2937 1730 599 24 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2884 3180 4704 4737 5106 5223 6056 844 Size: 2040 296 1524 33 369 117 833 3489 Fragments arranged by size: 3489 2040 1524 833 369 296 117 33 BsrBI CCG'CTC Cuts at: 1394 1823 2890 3217 3250 3328 4141 4300 4383 Size: 429 1067 327 33 78 813 159 83 Cuts at: 4383 4426 4690 5380 5835 5908 5940 6588 1394 Size: 43 264 690 455 73 32 648 3507 Fragments arranged by size: 3507 1067 813 690 648 455 429 327 264 159 83 78 73 43 33 32 BsrDI GCAATG_nn' Cuts at: 65 1248 2107 4720 7196 7370 65 Size: 1183 859 2613 2476 174 1396 Fragments arranged by size: 2613 2476 1396 1183 859 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3715 4395 7345 7532 1700 Size: 110 1905 680 2950 187 2869 Fragments arranged by size: 2950 2869 1905 680 187 110 BsrGI T'GTAC_A Cuts at: 96 2121 96 Size: 2025 6676 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8701 BssSI C'ACGA_G Cuts at: 4821 6761 7773 4821 Size: 1940 1012 5749 Fragments arranged by size: 5749 1940 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2479 2719 3127 3310 3493 3884 4085 794 Size: 1685 240 408 183 183 391 201 5410 Fragments arranged by size: 5410 1685 408 391 240 201 183 183 BstBI TT'CG_AA Cuts at: 7505 7505 Size: 8701 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8701 BstXI CCAn_nnnn'nTGG Cuts at: 2680 4823 2680 Size: 2143 6558 BstYI r'GATC_y Cuts at: 1972 2073 2412 4870 4878 5034 5067 5829 6031 Size: 101 339 2458 8 156 33 762 202 Cuts at: 6031 6398 6783 6800 8252 8338 8350 8696 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2458 1977 1452 762 385 367 346 339 202 156 101 86 33 17 12 8 Bsu36I CC'TnA_GG Cuts at: 2417 4214 2417 Size: 1797 6904 BtgI C'CryG_G Cuts at: 513 1065 2004 2547 2841 2883 3001 4464 4599 Size: 552 939 543 294 42 118 1463 135 Cuts at: 4599 4659 5331 5415 513 Size: 60 672 84 3799 Fragments arranged by size: 3799 1463 939 672 552 543 294 135 118 84 60 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2233 2587 4587 5383 487 Size: 683 56 1007 354 2000 796 3805 Fragments arranged by size: 3805 2000 1007 796 683 354 56 BtsI GCAGTG_nn' Cuts at: 3882 4032 4507 4903 5759 6217 7011 7031 3882 Size: 150 475 396 856 458 794 20 5552 Fragments arranged by size: 5552 856 794 475 458 396 150 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8701 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8666 DraI TTT'AAA Cuts at: 3111 6005 3111 Size: 2894 5807 DraIII CAC_nnn'GTG Cuts at: 2449 2449 Size: 8701 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5705 7708 809 Size: 507 4389 2003 1802 Fragments arranged by size: 4389 2003 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3615 4067 4388 Size: 54 1425 50 275 18 1785 452 321 Cuts at: 4388 6148 7037 8666 8 Size: 1760 889 1629 43 Fragments arranged by size: 1785 1760 1629 1425 889 452 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6148 1537 Size: 275 4336 4090 Fragments arranged by size: 4336 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2190 2273 2735 3570 4677 6631 1353 Size: 837 83 462 835 1107 1954 3423 Fragments arranged by size: 3423 1954 1107 837 835 462 83 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2041 2742 3353 3905 3968 4096 Size: 1064 138 452 701 611 552 63 128 Cuts at: 4096 7288 7662 7808 387 Size: 3192 374 146 1280 Fragments arranged by size: 3192 1280 1064 701 611 552 452 374 146 138 128 63 EcoICRI GAG'CTC Cuts at: 4851 5412 5882 4851 Size: 561 470 7670 Fragments arranged by size: 7670 561 470 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2399 2577 2876 3062 3063 3314 3383 Size: 393 767 178 299 186 1 251 69 Cuts at: 3383 3392 3452 3791 3960 4143 4316 4701 4911 Size: 9 60 339 169 183 173 385 210 Cuts at: 4911 5222 5536 6764 7361 8148 8416 1239 Size: 311 314 1228 597 787 268 1524 Fragments arranged by size: 1524 1228 787 767 597 393 385 339 314 311 299 268 251 210 186 183 178 173 169 69 60 9 1 EcoNI CCTnn'n_nnAGG Cuts at: 2381 4280 2381 Size: 1899 6802 EcoO109I rG'GnC_Cy Cuts at: 2062 3641 3674 3675 3683 3763 3944 3994 4017 Size: 1579 33 1 8 80 181 50 23 Cuts at: 4017 4062 4163 4164 4441 4632 4837 5434 2062 Size: 45 101 1 277 191 205 597 5329 Fragments arranged by size: 5329 1579 597 277 205 191 181 101 80 50 45 33 23 8 1 1 EcoRI G'AATT_C Cuts at: 3922 6010 6388 3922 Size: 2088 378 6235 Fragments arranged by size: 6235 2088 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2881 2881 Size: 8701 FseI GG_CCGG'CC Cuts at: 4399 4399 Size: 8701 FspI TGC'GCA Cuts at: 7207 7207 Size: 8701 HaeII r_GCGC'y Cuts at: 1678 3223 7848 1678 Size: 1545 4625 2531 Fragments arranged by size: 4625 2531 1545 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2256 2288 2639 2671 3894 Size: 32 135 32 684 32 351 32 1223 Cuts at: 3894 3897 3920 3926 3929 3952 4367 4399 4460 Size: 3 23 6 3 23 415 32 61 Cuts at: 4460 4492 4802 4834 5567 5599 7342 7374 7416 Size: 32 310 32 733 32 1743 32 42 Cuts at: 7416 7448 1373 Size: 32 2626 Fragments arranged by size: 2626 1743 1223 733 684 415 351 310 135 61 42 32 32 32 32 32 32 32 32 32 32 23 23 6 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 3051 4990 5095 5506 5581 5716 Size: 988 281 1113 1939 105 411 75 135 Cuts at: 5716 6045 6291 669 Size: 329 246 3079 Fragments arranged by size: 3079 1939 1113 988 411 329 281 246 135 105 75 HindIII A'AGCT_T Cuts at: 748 748 Size: 8701 HpaI GTT'AAC Cuts at: 6291 6291 Size: 8701 KasI G'GCGC_C Cuts at: 3219 3219 Size: 8701 KpnI G_GTAC'C Cuts at: 6026 6026 Size: 8701 MfeI C'AATT_G Cuts at: 6300 6300 Size: 8701 MluI A'CGCG_T Cuts at: 8577 8577 Size: 8701 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5817 7815 7999 1305 Size: 4512 1998 184 2007 Fragments arranged by size: 4512 2007 1998 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3617 10 Size: 54 1425 343 1785 5094 Fragments arranged by size: 5094 1785 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2436 2678 3035 4601 4661 5361 5612 Size: 648 1270 242 357 1566 60 700 251 Cuts at: 5612 5678 6659 7018 7177 518 Size: 66 981 359 159 2042 Fragments arranged by size: 2042 1566 1270 981 700 648 359 357 251 242 159 66 60 MspA1I CmG'CkG Cuts at: 1606 2152 2536 2976 3127 3184 3190 3241 3259 Size: 546 384 440 151 57 6 51 18 Cuts at: 3259 3286 3370 3382 3409 3545 4957 5530 6117 Size: 27 84 12 27 136 1412 573 587 Cuts at: 6117 6794 7942 8187 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1412 1148 677 587 573 546 440 384 245 151 136 84 57 51 27 27 18 12 6 NaeI GCC'GGC Cuts at: 4397 4397 Size: 8701 NarI GG'CG_CC Cuts at: 3220 3220 Size: 8701 NcoI C'CATG_G Cuts at: 513 1065 2004 3001 4464 4599 4659 513 Size: 552 939 997 1463 135 60 4555 Fragments arranged by size: 4555 1463 997 939 552 135 60 NdeI CA'TA_TG Cuts at: 387 3778 387 Size: 3391 5310 NgoMIV G'CCGG_C Cuts at: 4395 4395 Size: 8701 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8701 NotI GC'GGCC_GC Cuts at: 6148 6148 Size: 8701 NspI r_CATG'y Cuts at: 1132 1229 2138 2221 2713 2811 3304 3425 4706 Size: 97 909 83 492 98 493 121 1281 Cuts at: 4706 6059 7604 1132 Size: 1353 1545 2229 Fragments arranged by size: 2229 1545 1353 1281 909 493 492 121 98 97 83 PasI CC'CwG_GG Cuts at: 1348 3671 4352 4559 1348 Size: 2323 681 207 5490 Fragments arranged by size: 5490 2323 681 207 PciI A'CATG_T Cuts at: 2807 7600 2807 Size: 4793 3908 PflMI CCAn_nnn'nTGG Cuts at: 1108 2775 5191 5572 5803 1108 Size: 1667 2416 381 231 4006 Fragments arranged by size: 4006 2416 1667 381 231 PfoI T'CCnGG_A Cuts at: 2871 2930 2871 Size: 59 8642 PmeI GTTT'AAAC Cuts at: 6005 6005 Size: 8701 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4963 4995 6770 6802 8283 8315 4963 Size: 32 1775 32 1481 32 5349 Fragments arranged by size: 5349 1775 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2062 3683 3763 3944 4017 4062 4632 2062 Size: 1621 80 181 73 45 570 6131 Fragments arranged by size: 6131 1621 570 181 80 73 45 PshAI GACnn'nnGTC Cuts at: 1613 2808 1613 Size: 1195 7506 PsiI TTA'TAA Cuts at: 6271 6271 Size: 8701 PspOMI G'GGCC_C Cuts at: 3561 3674 4163 5013 5433 3561 Size: 113 489 850 420 6829 Fragments arranged by size: 6829 850 489 420 113 PstI C_TGCA'G Cuts at: 830 2408 3132 3270 3414 3444 3813 4962 5754 Size: 1578 724 138 144 30 369 1149 792 Cuts at: 5754 6053 830 Size: 299 3478 Fragments arranged by size: 3478 1578 1149 792 724 369 299 144 138 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8701 PvuII CAG'CTG Cuts at: 2152 2536 3127 3370 3409 4957 2152 Size: 384 591 243 39 1548 5896 Fragments arranged by size: 5896 1548 591 384 243 39 SacI G_AGCT'C Cuts at: 4853 5414 5884 4853 Size: 561 470 7670 Fragments arranged by size: 7670 561 470 SalI G'TCGA_C Cuts at: 1936 3049 5504 5579 6043 1936 Size: 1113 2455 75 464 4594 Fragments arranged by size: 4594 2455 1113 464 75 SanDI GG'GwC_CC Cuts at: 2062 3763 4062 4632 2062 Size: 1701 299 570 6131 Fragments arranged by size: 6131 1701 570 299 SapI GCTCTTCn'nnn_ Cuts at: 2273 2273 Size: 8701 SbfI CC_TGCA'GG Cuts at: 3813 6053 3813 Size: 2240 6461 ScaI AGT'ACT Cuts at: 2231 6949 8661 2231 Size: 4718 1712 2271 Fragments arranged by size: 4718 2271 1712 SexAI A'CCwGG_T Cuts at: 2349 2349 Size: 8701 SfcI C'TryA_G Cuts at: 826 1045 2404 3128 3266 3410 3440 3809 4958 Size: 219 1359 724 138 144 30 369 1149 Cuts at: 4958 5750 6049 7184 7865 8056 826 Size: 792 299 1135 681 191 1471 Fragments arranged by size: 1471 1359 1149 1135 792 724 681 369 299 219 191 144 138 30 SfiI GGCCn_nnn'nGGCC Cuts at: 4396 4396 Size: 8701 SfoI GGC'GCC Cuts at: 3221 3221 Size: 8701 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8701 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8701 SmaI CCC'GGG Cuts at: 1872 2289 5012 6028 1872 Size: 417 2723 1016 4545 Fragments arranged by size: 4545 2723 1016 417 SmlI C'TyrA_G Cuts at: 820 1017 1943 2762 2909 3431 5405 5534 5891 Size: 197 926 819 147 522 1974 129 357 Cuts at: 5891 6805 7706 7968 820 Size: 914 901 262 1553 Fragments arranged by size: 1974 1553 926 914 901 819 522 357 262 197 147 129 SnaBI TAC'GTA Cuts at: 493 6492 493 Size: 5999 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8701 SphI G_CATG'C Cuts at: 1132 3425 4706 6059 1132 Size: 2293 1281 1353 3774 Fragments arranged by size: 3774 2293 1353 1281 SspI AAT'ATT Cuts at: 5 52 6625 5 Size: 47 6573 2081 Fragments arranged by size: 6573 2081 47 StuI AGG'CCT Cuts at: 1517 3665 1517 Size: 2148 6553 StyI C'CwwG_G Cuts at: 513 1065 2004 3001 3612 4002 4464 4599 4659 Size: 552 939 997 611 390 462 135 60 Cuts at: 4659 6140 513 Size: 1481 3074 Fragments arranged by size: 3074 1481 997 939 611 552 462 390 135 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2854 2940 3895 4015 4774 4919 5151 Size: 1255 1274 86 955 120 759 145 232 Cuts at: 5151 6728 6745 6898 7083 7528 325 Size: 1577 17 153 185 445 1498 Fragments arranged by size: 1577 1498 1274 1255 955 759 445 232 185 153 145 120 86 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2121 2229 2970 Size: 274 80 33 51 1443 144 108 741 Cuts at: 2970 5043 6947 8659 96 Size: 2073 1904 1712 138 Fragments arranged by size: 2073 1904 1712 1443 741 274 144 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3747 3779 8661 8693 3747 Size: 32 4882 32 3755 Fragments arranged by size: 4882 3755 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4250 4589 4739 5861 1316 Size: 2934 339 150 1122 4156 Fragments arranged by size: 4156 2934 1122 339 150 XbaI T'CTAG_A Cuts at: 6037 6037 Size: 8701 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 3829 4462 4489 5800 2167 Size: 1662 633 27 1311 5068 Fragments arranged by size: 5068 1662 1311 633 27 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8701 XmaI C'CCGG_G Cuts at: 1870 2287 5010 6026 1870 Size: 417 2723 1016 4545 Fragments arranged by size: 4545 2723 1016 417 XmnI GAAnn'nnTTC Cuts at: 2564 5054 6830 2564 Size: 2490 1776 4435 Fragments arranged by size: 4435 2490 1776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4615 6509 276 Size: 53 83 186 936 3081 1894 2468 Fragments arranged by size: 3081 2468 1894 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BstZ17I EcoRV FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SrfI SwaI