(Linear) (Six-base) MAPSORT of: KIEE3608.seq Check: 4011 from: 1 to: 3996 KIEE3608.orf With 182 enzymes: SgfI * March 22, 2012 16:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1175 3996 Size: 1175 2821 AatII G_ACGT'C Cuts at: 0 2612 3996 Size: 2612 1384 AccI GT'mk_AC Cuts at: 0 1045 3500 3575 3704 3996 Size: 1045 2455 75 129 292 Fragments arranged by size: 2455 1045 292 129 75 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 394 871 1447 1786 1955 2696 3996 Size: 394 477 576 339 169 741 1300 Fragments arranged by size: 1300 741 576 477 394 339 169 AflII C'TTAA_G Cuts at: 0 904 3996 Size: 904 3092 AflIII A'CryG_T Cuts at: 0 802 3713 3996 Size: 802 2911 283 Fragments arranged by size: 2911 802 283 AhdI GACnn_n'nnGTC Cuts at: 0 2469 2811 3996 Size: 2469 342 1185 Fragments arranged by size: 2469 1185 342 AleI CACnn'nnGTG Cuts at: 0 2596 2656 3356 3996 Size: 2596 60 700 640 Fragments arranged by size: 2596 700 640 60 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 167 1433 3691 3996 Size: 167 1266 2258 305 Fragments arranged by size: 2258 1266 305 167 AlwNI CAG_nnn'CTG Cuts at: 0 340 849 1122 1305 1488 1783 1803 3996 Size: 340 509 273 183 183 295 20 2193 Fragments arranged by size: 2193 509 340 295 273 183 183 20 ApaI G_GGCC'C Cuts at: 0 1560 1673 2162 3012 3432 3996 Size: 1560 113 489 850 420 564 Fragments arranged by size: 1560 850 564 489 420 113 ApaLI G'TGCA_C Cuts at: 0 3610 3996 Size: 3610 386 ApoI r'AATT_y Cuts at: 0 1917 3448 3996 Size: 1917 1531 548 Fragments arranged by size: 1917 1531 548 AvaI C'yCGr_G Cuts at: 0 282 1147 1559 1895 3005 3579 3996 Size: 282 865 412 336 1110 574 417 Fragments arranged by size: 1110 865 574 417 412 336 282 BanI G'GyrC_C Cuts at: 0 1214 2001 2140 2267 2650 3996 Size: 1214 787 139 127 383 1346 Fragments arranged by size: 1346 1214 787 383 139 127 BanII G_rGCy'C Cuts at: 0 280 690 723 1560 1646 1673 1721 2162 Size: 280 410 33 837 86 27 48 441 Cuts at: 2162 2848 3012 3206 3409 3432 3879 3996 Size: 686 164 194 203 23 447 117 Fragments arranged by size: 837 686 447 441 410 280 203 194 164 117 86 48 33 27 23 BbeI G_GCGC'C Cuts at: 0 1218 3996 Size: 1218 2778 BbsI GAAGACnn'nnnn_ Cuts at: 0 3852 3996 Size: 3852 144 BbvCI CC'TCA_GC Cuts at: 0 494 3996 Size: 494 3502 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2925 2959 3996 Size: 2925 34 1037 Fragments arranged by size: 2925 1037 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2568 3996 Size: 2568 1428 BglI GCCn_nnn'nGGC Cuts at: 0 2157 2391 3996 Size: 2157 234 1605 Fragments arranged by size: 2157 1605 234 BlpI GC'TnA_GC Cuts at: 0 527 2168 3996 Size: 527 1641 1828 Fragments arranged by size: 1828 1641 527 Bme1580I G_kGCm'C Cuts at: 0 1560 1673 1896 2143 2162 2270 2938 3012 Size: 1560 113 223 247 19 108 668 74 Cuts at: 3012 3106 3353 3432 3614 3996 Size: 94 247 79 182 382 Fragments arranged by size: 1560 668 382 247 247 223 182 113 108 94 79 74 19 BmgBI CAC'GTC Cuts at: 0 2733 2964 3996 Size: 2733 231 1032 Fragments arranged by size: 2733 1032 231 BmrI ACTGGGnnnn_n' Cuts at: 0 1791 1883 3996 Size: 1791 92 2113 Fragments arranged by size: 2113 1791 92 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1035 3846 3996 Size: 1035 2811 150 Fragments arranged by size: 2811 1035 150 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 572 1057 1058 1309 1378 1387 2138 2311 Size: 572 485 1 251 69 9 751 173 Cuts at: 2311 2906 3217 3531 3996 Size: 595 311 314 465 Fragments arranged by size: 751 595 572 485 465 314 311 251 173 69 9 1 Bpu10I CC'TnA_GC Cuts at: 0 494 823 1640 3996 Size: 494 329 817 2356 Fragments arranged by size: 2356 817 494 329 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 742 1411 3385 3514 3871 3996 Size: 742 669 1974 129 357 125 Fragments arranged by size: 1974 742 669 357 129 125 BsaI GGTCTCn'nnnn_ Cuts at: 0 656 1136 3013 3996 Size: 656 480 1877 983 Fragments arranged by size: 1877 983 656 480 BsaAI yAC'GTr Cuts at: 0 3716 3996 Size: 3716 280 BsaHI Gr'CG_yC Cuts at: 0 1215 2609 3996 Size: 1215 1394 1387 Fragments arranged by size: 1394 1387 1215 BsaWI w'CCGG_w Cuts at: 0 3032 3269 3996 Size: 3032 237 727 Fragments arranged by size: 3032 727 237 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 3769 3799 3996 Size: 3769 30 197 Fragments arranged by size: 3769 197 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 199 964 994 1210 1222 1240 1330 1333 Size: 199 765 30 216 12 18 90 3 Cuts at: 1333 1384 1685 1913 2229 2304 2497 2524 2629 Size: 51 301 228 316 75 193 27 105 Cuts at: 2629 2695 3529 3996 Size: 66 834 467 Fragments arranged by size: 834 765 467 316 301 228 216 199 193 105 90 75 66 51 30 27 18 12 3 BseYI C'CCAG_C Cuts at: 0 9 64 1839 1948 1962 2228 2630 2743 Size: 9 55 1775 109 14 266 402 113 Cuts at: 2743 3803 3996 Size: 1060 193 Fragments arranged by size: 1775 1060 402 266 193 113 109 55 14 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1156 3092 3268 3996 Size: 1156 1936 176 728 Fragments arranged by size: 1936 1156 728 176 BsiHKAI G_wGCw'C Cuts at: 0 528 2342 2848 3409 3614 3879 3996 Size: 528 1814 506 561 205 265 117 Fragments arranged by size: 1814 561 528 506 265 205 117 BsiWI C'GTAC_G Cuts at: 0 3716 3996 Size: 3716 280 BsmI GAATG_Cn' Cuts at: 0 495 3996 Size: 495 3501 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1396 3832 3996 Size: 1396 2436 164 Fragments arranged by size: 2436 1396 164 Bsp1286I G_dGCh'C Cuts at: 0 280 528 690 723 1560 1646 1673 1721 Size: 280 248 162 33 837 86 27 48 Cuts at: 1721 1896 2143 2162 2270 2342 2848 2938 3012 Size: 175 247 19 108 72 506 90 74 Cuts at: 3012 3106 3206 3353 3409 3432 3614 3879 3996 Size: 94 100 147 56 23 182 265 117 Fragments arranged by size: 837 506 280 265 248 247 182 175 162 147 117 108 100 94 90 86 74 72 56 48 33 27 23 19 BspEI T'CCGG_A Cuts at: 0 3032 3269 3996 Size: 3032 237 727 Fragments arranged by size: 3032 727 237 BspHI T'CATG_A Cuts at: 0 1025 3962 3986 3996 Size: 1025 2937 24 10 Fragments arranged by size: 2937 1025 24 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 879 1175 2699 2732 3101 3218 3996 Size: 879 296 1524 33 369 117 778 Fragments arranged by size: 1524 879 778 369 296 117 33 BsrBI CCG'CTC Cuts at: 0 885 1212 1245 1323 2136 2295 2378 2421 Size: 885 327 33 78 813 159 83 43 Cuts at: 2421 2685 3375 3830 3903 3935 3996 Size: 264 690 455 73 32 61 Fragments arranged by size: 885 813 690 455 327 264 159 83 78 73 61 43 33 32 BsrDI GCAATG_nn' Cuts at: 0 102 2715 3996 Size: 102 2613 1281 Fragments arranged by size: 2613 1281 102 BsrFI r'CCGG_y Cuts at: 0 1710 2390 3996 Size: 1710 680 1606 Fragments arranged by size: 1710 1606 680 BsrGI T'GTAC_A Cuts at: 0 116 3996 Size: 116 3880 BssSI C'ACGA_G Cuts at: 0 2816 3996 Size: 2816 1180 BstAPI GCAn_nnn'nTGC Cuts at: 0 474 714 1122 1305 1488 1879 2080 3996 Size: 474 240 408 183 183 391 201 1916 Fragments arranged by size: 1916 474 408 391 240 201 183 183 BstXI CCAn_nnnn'nTGG Cuts at: 0 675 2818 3996 Size: 675 2143 1178 Fragments arranged by size: 2143 1178 675 BstYI r'GATC_y Cuts at: 0 68 407 2865 2873 3029 3062 3824 3996 Size: 68 339 2458 8 156 33 762 172 Fragments arranged by size: 2458 762 339 172 156 68 33 8 Bsu36I CC'TnA_GG Cuts at: 0 412 2209 3996 Size: 412 1797 1787 Fragments arranged by size: 1797 1787 412 BtgI C'CryG_G Cuts at: 0 542 836 878 996 2459 2594 2654 3326 Size: 542 294 42 118 1463 135 60 672 Cuts at: 3326 3410 3996 Size: 84 586 Fragments arranged by size: 1463 672 586 542 294 135 118 84 60 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 228 582 2582 3378 3996 Size: 228 354 2000 796 618 Fragments arranged by size: 2000 796 618 354 228 BtsI GCAGTG_nn' Cuts at: 0 1877 2027 2502 2898 3754 3996 Size: 1877 150 475 396 856 242 Fragments arranged by size: 1877 856 475 396 242 150 DraI TTT'AAA Cuts at: 0 1106 3996 Size: 1106 2890 DraIII CAC_nnn'GTG Cuts at: 0 444 3996 Size: 444 3552 DrdI GACnn_nn'nnGTC Cuts at: 0 3700 3996 Size: 3700 296 EaeI y'GGCC_r Cuts at: 0 1610 2062 2383 3996 Size: 1610 452 321 1613 Fragments arranged by size: 1613 1610 452 321 EarI CTCTTCn'nnn_ Cuts at: 0 185 268 730 1565 2672 3996 Size: 185 83 462 835 1107 1324 Fragments arranged by size: 1324 1107 835 462 185 83 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 36 737 1348 1900 1963 2091 3996 Size: 36 701 611 552 63 128 1905 Fragments arranged by size: 1905 701 611 552 128 63 36 EcoICRI GAG'CTC Cuts at: 0 2846 3407 3877 3996 Size: 2846 561 470 119 Fragments arranged by size: 2846 561 470 119 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 394 572 871 1057 1058 1309 1378 1387 Size: 394 178 299 186 1 251 69 9 Cuts at: 1387 1447 1786 1955 2138 2311 2696 2906 3217 Size: 60 339 169 183 173 385 210 311 Cuts at: 3217 3531 3996 Size: 314 465 Fragments arranged by size: 465 394 385 339 314 311 299 251 210 186 183 178 173 169 69 60 9 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 376 2275 3996 Size: 376 1899 1721 Fragments arranged by size: 1899 1721 376 EcoO109I rG'GnC_Cy Cuts at: 0 57 1636 1669 1670 1678 1758 1939 1989 Size: 57 1579 33 1 8 80 181 50 Cuts at: 1989 2012 2057 2158 2159 2436 2627 2832 3429 Size: 23 45 101 1 277 191 205 597 Cuts at: 3429 3996 Size: 567 Fragments arranged by size: 1579 597 567 277 205 191 181 101 80 57 50 45 33 23 8 1 1 EcoRI G'AATT_C Cuts at: 0 1917 3996 Size: 1917 2079 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 876 3996 Size: 876 3120 FseI GG_CCGG'CC Cuts at: 0 2394 3996 Size: 2394 1602 HaeII r_GCGC'y Cuts at: 0 1218 3996 Size: 1218 2778 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 251 283 634 666 1889 1892 1915 1921 Size: 251 32 351 32 1223 3 23 6 Cuts at: 1921 1924 1947 2362 2394 2455 2487 2797 2829 Size: 3 23 415 32 61 32 310 32 Cuts at: 2829 3562 3594 3996 Size: 733 32 402 Fragments arranged by size: 1223 733 415 402 351 310 251 61 32 32 32 32 32 32 23 23 6 3 3 HincII GTy'rAC Cuts at: 0 1046 2985 3090 3501 3576 3711 3996 Size: 1046 1939 105 411 75 135 285 Fragments arranged by size: 1939 1046 411 285 135 105 75 KasI G'GCGC_C Cuts at: 0 1214 3996 Size: 1214 2782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 3812 3996 Size: 3812 184 MscI TGG'CCA Cuts at: 0 1612 3996 Size: 1612 2384 MslI CAynn'nnrTG Cuts at: 0 431 673 1030 2596 2656 3356 3607 3673 Size: 431 242 357 1566 60 700 251 66 Cuts at: 3673 3996 Size: 323 Fragments arranged by size: 1566 700 431 357 323 251 242 66 60 MspA1I CmG'CkG Cuts at: 0 147 531 971 1122 1179 1185 1236 1254 Size: 147 384 440 151 57 6 51 18 Cuts at: 1254 1281 1365 1377 1404 1540 2952 3525 3996 Size: 27 84 12 27 136 1412 573 471 Fragments arranged by size: 1412 573 471 440 384 151 147 136 84 57 51 27 27 18 12 6 NaeI GCC'GGC Cuts at: 0 2392 3996 Size: 2392 1604 NarI GG'CG_CC Cuts at: 0 1215 3996 Size: 1215 2781 NcoI C'CATG_G Cuts at: 0 996 2459 2594 2654 3996 Size: 996 1463 135 60 1342 Fragments arranged by size: 1463 1342 996 135 60 NdeI CA'TA_TG Cuts at: 0 1773 3996 Size: 1773 2223 NgoMIV G'CCGG_C Cuts at: 0 2390 3996 Size: 2390 1606 NspI r_CATG'y Cuts at: 0 133 216 708 806 1299 1420 2701 3996 Size: 133 83 492 98 493 121 1281 1295 Fragments arranged by size: 1295 1281 493 492 133 121 98 83 PasI CC'CwG_GG Cuts at: 0 1666 2347 2554 3996 Size: 1666 681 207 1442 Fragments arranged by size: 1666 1442 681 207 PciI A'CATG_T Cuts at: 0 802 3996 Size: 802 3194 PflMI CCAn_nnn'nTGG Cuts at: 0 770 3186 3567 3798 3996 Size: 770 2416 381 231 198 Fragments arranged by size: 2416 770 381 231 198 PfoI T'CCnGG_A Cuts at: 0 866 925 3996 Size: 866 59 3071 Fragments arranged by size: 3071 866 59 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2958 2990 3996 Size: 2958 32 1006 Fragments arranged by size: 2958 1006 32 PpuMI rG'GwC_Cy Cuts at: 0 57 1678 1758 1939 2012 2057 2627 3996 Size: 57 1621 80 181 73 45 570 1369 Fragments arranged by size: 1621 1369 570 181 80 73 57 45 PshAI GACnn'nnGTC Cuts at: 0 803 3996 Size: 803 3193 PspOMI G'GGCC_C Cuts at: 0 1556 1669 2158 3008 3428 3996 Size: 1556 113 489 850 420 568 Fragments arranged by size: 1556 850 568 489 420 113 PstI C_TGCA'G Cuts at: 0 403 1127 1265 1409 1439 1808 2957 3749 Size: 403 724 138 144 30 369 1149 792 Cuts at: 3749 3996 Size: 247 Fragments arranged by size: 1149 792 724 403 369 247 144 138 30 PvuII CAG'CTG Cuts at: 0 147 531 1122 1365 1404 2952 3996 Size: 147 384 591 243 39 1548 1044 Fragments arranged by size: 1548 1044 591 384 243 147 39 SacI G_AGCT'C Cuts at: 0 2848 3409 3879 3996 Size: 2848 561 470 117 Fragments arranged by size: 2848 561 470 117 SalI G'TCGA_C Cuts at: 0 1044 3499 3574 3996 Size: 1044 2455 75 422 Fragments arranged by size: 2455 1044 422 75 SanDI GG'GwC_CC Cuts at: 0 57 1758 2057 2627 3996 Size: 57 1701 299 570 1369 Fragments arranged by size: 1701 1369 570 299 57 SapI GCTCTTCn'nnn_ Cuts at: 0 268 3996 Size: 268 3728 SbfI CC_TGCA'GG Cuts at: 0 1808 3996 Size: 1808 2188 ScaI AGT'ACT Cuts at: 0 226 3996 Size: 226 3770 SexAI A'CCwGG_T Cuts at: 0 344 3996 Size: 344 3652 SfcI C'TryA_G Cuts at: 0 399 1123 1261 1405 1435 1804 2953 3745 Size: 399 724 138 144 30 369 1149 792 Cuts at: 3745 3996 Size: 251 Fragments arranged by size: 1149 792 724 399 369 251 144 138 30 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2391 3996 Size: 2391 1605 SfoI GGC'GCC Cuts at: 0 1216 3996 Size: 1216 2780 SmaI CCC'GGG Cuts at: 0 284 3007 3996 Size: 284 2723 989 Fragments arranged by size: 2723 989 284 SmlI C'TyrA_G Cuts at: 0 757 904 1426 3400 3529 3886 3996 Size: 757 147 522 1974 129 357 110 Fragments arranged by size: 1974 757 522 357 147 129 110 SphI G_CATG'C Cuts at: 0 1420 2701 3996 Size: 1420 1281 1295 Fragments arranged by size: 1420 1295 1281 StuI AGG'CCT Cuts at: 0 1660 3996 Size: 1660 2336 StyI C'CwwG_G Cuts at: 0 996 1607 1997 2459 2594 2654 3996 Size: 996 611 390 462 135 60 1342 Fragments arranged by size: 1342 996 611 462 390 135 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 849 935 1890 2010 2769 2914 3146 3996 Size: 849 86 955 120 759 145 232 850 Fragments arranged by size: 955 850 849 759 232 145 120 86 TatI w'GTAC_w Cuts at: 0 116 224 965 3038 3996 Size: 116 108 741 2073 958 Fragments arranged by size: 2073 958 741 116 108 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1742 1774 3996 Size: 1742 32 2222 Fragments arranged by size: 2222 1742 32 Tth111I GACn'n_nGTC Cuts at: 0 2245 2584 2734 3856 3996 Size: 2245 339 150 1122 140 Fragments arranged by size: 2245 1122 339 150 140 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 162 1824 2457 2484 3795 3996 Size: 162 1662 633 27 1311 201 Fragments arranged by size: 1662 1311 633 201 162 27 XmaI C'CCGG_G Cuts at: 0 282 3005 3996 Size: 282 2723 991 Fragments arranged by size: 2723 991 282 XmnI GAAnn'nnTTC Cuts at: 0 559 3049 3996 Size: 559 2490 947 Fragments arranged by size: 2490 947 559 ZraI GAC'GTC Cuts at: 0 2610 3996 Size: 2610 1386 Enzymes that do cut: AarI AatII AccI AcuI AflII AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AgeI AloI AscI AseI AsiSI AvrII BaeI BamHI BclI BfrBI BglII BmtI BsaBI BsiEI BssHII BstBI BstEII BstZ17I ClaI CspCI EagI EcoRV FspI FspAI HindIII HpaI KpnI MfeI MluI NheI NotI NruI NsiI PacI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacII SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XhoI