(Circular) (Six-base) MAPSORT of: pFN21AE2276.seq Check: 2397 from: 1 to: 6397 pFN21AE2276 6397 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4207 278 Size: 53 83 186 936 2671 2468 Fragments arranged by size: 2671 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3740 1937 Size: 1803 4594 Acc65I G'GTAC_C Cuts at: 3069 3718 3069 Size: 649 5748 AclI AA'CG_TT Cuts at: 1563 4524 4897 1563 Size: 2961 373 3063 Fragments arranged by size: 3063 2961 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2086 2834 3359 4460 5844 6112 1632 Size: 454 748 525 1101 1384 268 1917 Fragments arranged by size: 1917 1384 1101 748 525 454 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6200 AflIII A'CryG_T Cuts at: 1203 5296 6273 1203 Size: 4093 977 1327 Fragments arranged by size: 4093 1327 977 AgeI A'CCGG_T Cuts at: 5228 5228 Size: 6397 AhdI GACnn_n'nnGTC Cuts at: 5126 5126 Size: 6397 AleI CACnn'nnGTG Cuts at: 1166 2954 1166 Size: 1788 4609 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3162 1184 Size: 1978 4419 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2627 2659 2627 Size: 32 6365 AlwNI CAG_nnn'CTG Cuts at: 2632 2737 3175 3325 3355 4140 5712 2632 Size: 105 438 150 30 785 1572 3317 Fragments arranged by size: 3317 1572 785 438 150 105 30 ApaI G_GGCC'C Cuts at: 2378 2378 Size: 6397 ApaLI G'TGCA_C Cuts at: 4454 5610 4454 Size: 1156 5241 ApoI r'AATT_y Cuts at: 1493 2801 3706 3932 4084 6193 1493 Size: 1308 905 226 152 2109 1697 Fragments arranged by size: 2109 1697 1308 905 226 152 AseI AT'TA_AT Cuts at: 160 1032 4951 160 Size: 872 3919 1606 Fragments arranged by size: 3919 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6397 AvaI C'yCGr_G Cuts at: 1870 1943 3722 1870 Size: 73 1779 4545 Fragments arranged by size: 4545 1779 73 BamHI G'GATC_C Cuts at: 3727 4094 3727 Size: 367 6030 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2288 2609 3069 3500 3718 Size: 325 217 627 501 321 460 431 218 Cuts at: 3718 5173 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 501 460 431 325 321 218 217 BanII G_rGCy'C Cuts at: 1391 2204 2378 3716 1391 Size: 813 174 1338 4072 Fragments arranged by size: 4072 1338 813 174 BbeI G_GCGC'C Cuts at: 2292 2292 Size: 6397 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5426 BbvCI CC'TCA_GC Cuts at: 3009 3054 3620 3009 Size: 45 566 5786 Fragments arranged by size: 5786 566 45 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4588 4622 1903 Size: 34 2651 34 3678 Fragments arranged by size: 3678 2651 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4289 5499 4289 Size: 1210 5187 BclI T'GATC_A Cuts at: 1548 1548 Size: 6397 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2097 2148 2199 Size: 107 122 71 631 605 425 51 51 Cuts at: 2199 5008 136 Size: 2809 1525 Fragments arranged by size: 2809 1525 631 605 425 122 107 71 51 51 BglII A'GATC_T Cuts at: 3179 6392 3179 Size: 3213 3184 BlpI GC'TnA_GC Cuts at: 3240 3814 3240 Size: 574 5823 Bme1580I G_kGCm'C Cuts at: 1790 2378 2396 2612 4458 5614 1790 Size: 588 18 216 1846 1156 2573 Fragments arranged by size: 2573 1846 1156 588 216 18 BmgBI CAC'GTC Cuts at: 1318 3196 1318 Size: 1878 4519 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3618 4128 5086 Size: 416 53 83 399 24 2201 510 958 Cuts at: 5086 442 Size: 1753 Fragments arranged by size: 2201 1753 958 510 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6397 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2110 2421 2654 3314 3431 3469 3658 5057 Size: 871 311 233 660 117 38 189 1399 Cuts at: 5057 1239 Size: 2579 Fragments arranged by size: 2579 1399 871 660 311 233 189 117 38 Bpu10I CC'TnA_GC Cuts at: 3009 3054 3523 3620 3009 Size: 45 469 97 5786 Fragments arranged by size: 5786 469 97 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2735 3248 4522 5387 5685 2735 Size: 513 1274 865 298 3447 Fragments arranged by size: 3447 1274 865 513 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5060 882 Size: 617 3561 2219 Fragments arranged by size: 3561 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4188 493 Size: 711 2984 2702 Fragments arranged by size: 2984 2702 711 BsaBI GATnn'nnATC Cuts at: 6391 6391 Size: 6397 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2289 4204 4586 5221 Size: 53 83 186 936 756 1915 382 635 Cuts at: 5221 275 Size: 1451 Fragments arranged by size: 1915 1451 936 756 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4830 5228 5502 5649 6060 4830 Size: 398 274 147 411 5167 Fragments arranged by size: 5167 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2627 2657 1178 Size: 30 165 30 1224 30 4918 Fragments arranged by size: 4918 1224 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2175 2504 3044 3058 3101 3434 3658 3661 Size: 989 329 540 14 43 333 224 3 Cuts at: 3661 1186 Size: 3922 Fragments arranged by size: 3922 989 540 333 329 224 43 14 3 BseYI C'CCAG_C Cuts at: 5600 6096 5600 Size: 496 5901 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3445 3491 1163 Size: 573 1709 46 4069 Fragments arranged by size: 4069 1709 573 46 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3847 4608 5636 1540 Size: 275 186 1846 761 1028 2301 Fragments arranged by size: 2301 1846 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4458 4543 5614 4458 Size: 85 1071 5241 Fragments arranged by size: 5241 1071 85 BsmI GAATG_Cn' Cuts at: 3908 4001 3908 Size: 93 6304 BsmBI CGTCTCn'nnnn_ Cuts at: 3487 3487 Size: 6397 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2204 2378 2396 2612 3716 4458 4543 Size: 399 414 174 18 216 1104 742 85 Cuts at: 4543 5614 1391 Size: 1071 2174 Fragments arranged by size: 2174 1104 1071 742 414 399 216 174 85 18 BspEI T'CCGG_A Cuts at: 6060 6060 Size: 6397 BspHI T'CATG_A Cuts at: 4286 6016 4286 Size: 1730 4667 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2460 3132 3752 844 Size: 1616 672 620 3489 Fragments arranged by size: 3489 1616 672 620 BsrBI CCG'CTC Cuts at: 1394 1823 2094 2124 4284 1394 Size: 429 271 30 2160 3507 Fragments arranged by size: 3507 2160 429 271 30 BsrDI GCAATG_nn' Cuts at: 65 1248 3228 4892 5066 65 Size: 1183 1980 1664 174 1396 Fragments arranged by size: 1980 1664 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2321 2351 5041 5228 1700 Size: 110 511 30 2690 187 2869 Fragments arranged by size: 2869 2690 511 187 110 30 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6397 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6397 BssSI C'ACGA_G Cuts at: 4457 5469 4457 Size: 1012 5385 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6397 BstBI TT'CG_AA Cuts at: 5201 5201 Size: 6397 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6397 BstYI r'GATC_y Cuts at: 1972 2211 3179 3727 4094 4479 4496 5948 6034 Size: 239 968 548 367 385 17 1452 86 Cuts at: 6034 6046 6392 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 968 548 385 367 346 239 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2666 2666 Size: 6397 BtgI C'CryG_G Cuts at: 513 1065 2004 2955 2967 513 Size: 552 939 951 12 3943 Fragments arranged by size: 3943 951 939 552 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2989 487 Size: 683 56 1763 3895 Fragments arranged by size: 3895 1763 683 56 BtsI GCAGTG_nn' Cuts at: 2140 3212 3523 3565 3913 4707 4727 2140 Size: 1072 311 42 348 794 20 3810 Fragments arranged by size: 3810 1072 794 348 311 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6397 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6362 DraI TTT'AAA Cuts at: 3701 3701 Size: 6397 DraIII CAC_nnn'GTG Cuts at: 2586 2586 Size: 6397 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5404 809 Size: 507 4088 1802 Fragments arranged by size: 4088 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3844 4733 Size: 54 1425 50 275 18 177 1837 889 Cuts at: 4733 6362 8 Size: 1629 43 Fragments arranged by size: 1837 1629 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3844 1537 Size: 275 2032 4090 Fragments arranged by size: 4090 2032 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3677 4327 1353 Size: 2324 650 3423 Fragments arranged by size: 3423 2324 650 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4984 5358 5504 387 Size: 1064 138 3395 374 146 1280 Fragments arranged by size: 3395 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2086 2110 2421 2654 2834 3314 3359 Size: 393 454 24 311 233 180 480 45 Cuts at: 3359 3431 3469 3658 4460 5057 5844 6112 1239 Size: 72 38 189 802 597 787 268 1524 Fragments arranged by size: 1524 802 787 597 480 454 393 311 268 233 189 180 72 45 38 24 EcoNI CCTnn'n_nnAGG Cuts at: 2597 2741 3308 3676 2597 Size: 144 567 368 5318 Fragments arranged by size: 5318 567 368 144 EcoO109I rG'GnC_Cy Cuts at: 2374 2375 2734 2746 3322 2374 Size: 1 359 12 576 5449 Fragments arranged by size: 5449 576 359 12 1 EcoRI G'AATT_C Cuts at: 3706 4084 3706 Size: 378 6019 FspI TGC'GCA Cuts at: 3128 4903 3128 Size: 1775 4622 HaeII r_GCGC'y Cuts at: 1678 2292 5544 1678 Size: 614 3252 2531 Fragments arranged by size: 3252 2531 614 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3047 3079 3171 3203 5038 Size: 32 135 32 1475 32 92 32 1835 Cuts at: 5038 5070 5112 5144 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1835 1475 135 92 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2425 3457 3741 3987 669 Size: 988 281 487 1032 284 246 3079 Fragments arranged by size: 3079 1032 988 487 284 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6397 HpaI GTT'AAC Cuts at: 3987 3987 Size: 6397 KasI G'GCGC_C Cuts at: 2288 2288 Size: 6397 KpnI G_GTAC'C Cuts at: 3073 3722 3073 Size: 649 5748 MfeI C'AATT_G Cuts at: 3996 3996 Size: 6397 MluI A'CGCG_T Cuts at: 6273 6273 Size: 6397 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3427 3553 3610 5511 5695 1305 Size: 2122 126 57 1901 184 2007 Fragments arranged by size: 2122 2007 1901 184 126 57 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4398 Fragments arranged by size: 4398 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2114 2954 4355 4714 4873 518 Size: 648 948 840 1401 359 159 2042 Fragments arranged by size: 2042 1401 948 840 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2232 2476 2677 3507 3597 3813 4490 5638 Size: 626 244 201 830 90 216 677 1148 Cuts at: 5638 5883 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 830 677 626 245 244 216 201 90 NaeI GCC'GGC Cuts at: 2323 2353 2323 Size: 30 6367 NarI GG'CG_CC Cuts at: 2289 2289 Size: 6397 NcoI C'CATG_G Cuts at: 513 1065 2004 2967 513 Size: 552 939 963 3943 Fragments arranged by size: 3943 963 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6397 NgoMIV G'CCGG_C Cuts at: 2321 2351 2321 Size: 30 6367 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6397 NotI GC'GGCC_GC Cuts at: 3844 3844 Size: 6397 NspI r_CATG'y Cuts at: 1132 1229 2157 2731 3755 5300 1132 Size: 97 928 574 1024 1545 2229 Fragments arranged by size: 2229 1545 1024 928 574 97 PasI CC'CwG_GG Cuts at: 1348 2081 1348 Size: 733 5664 PciI A'CATG_T Cuts at: 5296 5296 Size: 6397 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6397 PmeI GTTT'AAAC Cuts at: 3701 3701 Size: 6397 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2627 2659 4466 4498 5979 6011 2627 Size: 32 1807 32 1481 32 3013 Fragments arranged by size: 3013 1807 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2734 2746 3322 2734 Size: 12 576 5809 Fragments arranged by size: 5809 576 12 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6397 PsiI TTA'TAA Cuts at: 3967 3967 Size: 6397 PspOMI G'GGCC_C Cuts at: 2374 2374 Size: 6397 PstI C_TGCA'G Cuts at: 830 2601 3096 3309 3749 830 Size: 1771 495 213 440 3478 Fragments arranged by size: 3478 1771 495 440 213 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6397 PvuII CAG'CTG Cuts at: 2232 2677 2232 Size: 445 5952 SalI G'TCGA_C Cuts at: 1936 3739 1936 Size: 1803 4594 SapI GCTCTTCn'nnn_ Cuts at: 3677 3677 Size: 6397 SbfI CC_TGCA'GG Cuts at: 3749 3749 Size: 6397 ScaI AGT'ACT Cuts at: 4645 6357 4645 Size: 1712 4685 SexAI A'CCwGG_T Cuts at: 2736 2736 Size: 6397 SfcI C'TryA_G Cuts at: 826 1045 2597 3092 3218 3305 3465 3745 4880 Size: 219 1552 495 126 87 160 280 1135 Cuts at: 4880 5561 5752 826 Size: 681 191 1471 Fragments arranged by size: 1552 1471 1135 681 495 280 219 191 160 126 87 SfoI GGC'GCC Cuts at: 2290 2290 Size: 6397 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6397 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6397 SmaI CCC'GGG Cuts at: 1872 3724 1872 Size: 1852 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2750 3263 4501 5402 5664 820 Size: 197 926 807 513 1238 901 262 1553 Fragments arranged by size: 1553 1238 926 901 807 513 262 197 SnaBI TAC'GTA Cuts at: 493 4188 493 Size: 3695 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6397 SphI G_CATG'C Cuts at: 1132 3755 1132 Size: 2623 3774 SspI AAT'ATT Cuts at: 5 52 4321 5 Size: 47 4269 2081 Fragments arranged by size: 4269 2081 47 StuI AGG'CCT Cuts at: 1517 3528 1517 Size: 2011 4386 StyI C'CwwG_G Cuts at: 513 1065 2004 2862 2967 3836 513 Size: 552 939 858 105 869 3074 Fragments arranged by size: 3074 939 869 858 552 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2135 2330 3521 4424 4441 4594 4779 Size: 1255 555 195 1191 903 17 153 185 Cuts at: 4779 5224 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1191 903 555 445 195 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2652 3654 4643 Size: 274 80 33 51 1443 675 1002 989 Cuts at: 4643 6355 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1002 989 675 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2570 2602 6357 6389 2570 Size: 32 3755 32 2578 Fragments arranged by size: 3755 2578 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3194 1316 Size: 1878 4519 XbaI T'CTAG_A Cuts at: 3733 3733 Size: 6397 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3322 3322 Size: 6397 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6397 XmaI C'CCGG_G Cuts at: 1870 3722 1870 Size: 1852 4545 XmnI GAAnn'nnTTC Cuts at: 4526 4526 Size: 6397 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4205 276 Size: 53 83 186 936 2671 2468 Fragments arranged by size: 2671 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI