Restriction map for
pFN21AE2276
(Linear) (Six-base) MAPSORT of: KIEE2276.seq Check: 4563 from: 1 to: 1692
KIEE2276.orf
With 182 enzymes: SgfI *
July 15, 2010 18:56 ..
Acc65I G'GTAC_C
Cuts at: 0 1064 1692
Size: 1064 628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 829 1354 1692
Size: 81 748 525 338
Fragments arranged by size:
748 525 338 81
AleI CACnn'nnGTG
Cuts at: 0 949 1692
Size: 949 743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1157 1692
Size: 1157 535
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 622 654 1692
Size: 622 32 1038
Fragments arranged by size:
1038 622 32
AlwNI CAG_nnn'CTG
Cuts at: 0 627 732 1170 1320 1350 1692
Size: 627 105 438 150 30 342
Fragments arranged by size:
627 438 342 150 105 30
ApaI G_GGCC'C
Cuts at: 0 373 1692
Size: 373 1319
ApoI r'AATT_y
Cuts at: 0 796 1692
Size: 796 896
BanI G'GyrC_C
Cuts at: 0 283 604 1064 1495 1692
Size: 283 321 460 431 197
Fragments arranged by size:
460 431 321 283 197
BanII G_rGCy'C
Cuts at: 0 199 373 1692
Size: 199 174 1319
Fragments arranged by size:
1319 199 174
BbeI G_GCGC'C
Cuts at: 0 287 1692
Size: 287 1405
BbvCI CC'TCA_GC
Cuts at: 0 1004 1049 1615 1692
Size: 1004 45 566 77
Fragments arranged by size:
1004 566 77 45
BglI GCCn_nnn'nGGC
Cuts at: 0 92 143 194 1692
Size: 92 51 51 1498
Fragments arranged by size:
1498 92 51 51
BglII A'GATC_T
Cuts at: 0 1174 1692
Size: 1174 518
BlpI GC'TnA_GC
Cuts at: 0 1235 1692
Size: 1235 457
Bme1580I G_kGCm'C
Cuts at: 0 373 391 607 1692
Size: 373 18 216 1085
Fragments arranged by size:
1085 373 216 18
BmgBI CAC'GTC
Cuts at: 0 1191 1692
Size: 1191 501
BmrI ACTGGGnnnn_n'
Cuts at: 0 1613 1692
Size: 1613 79
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 416 649 1309 1426 1464 1653 1692
Size: 105 311 233 660 117 38 189 39
Fragments arranged by size:
660 311 233 189 117 105 39 38
Bpu10I CC'TnA_GC
Cuts at: 0 1004 1049 1518 1615 1692
Size: 1004 45 469 97 77
Fragments arranged by size:
1004 469 97 77 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 1243 1692
Size: 730 513 449
Fragments arranged by size:
730 513 449
BsaHI Gr'CG_yC
Cuts at: 0 284 1692
Size: 284 1408
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 622 652 1692
Size: 622 30 1040
Fragments arranged by size:
1040 622 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 170 499 1039 1053 1096 1429 1653 1656
Size: 170 329 540 14 43 333 224 3
Cuts at: 1656 1692
Size: 36
Fragments arranged by size:
540 333 329 224 170 43 36 14
3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1440 1486 1692
Size: 1440 46 206
Fragments arranged by size:
1440 206 46
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1482 1692
Size: 1482 210
Bsp1286I G_dGCh'C
Cuts at: 0 199 373 391 607 1692
Size: 199 174 18 216 1085
Fragments arranged by size:
1085 216 199 174 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 455 1127 1692
Size: 455 672 565
Fragments arranged by size:
672 565 455
BsrBI CCG'CTC
Cuts at: 0 89 119 1692
Size: 89 30 1573
Fragments arranged by size:
1573 89 30
BsrDI GCAATG_nn'
Cuts at: 0 1223 1692
Size: 1223 469
BsrFI r'CCGG_y
Cuts at: 0 316 346 1692
Size: 316 30 1346
Fragments arranged by size:
1346 316 30
BstYI r'GATC_y
Cuts at: 0 206 1174 1692
Size: 206 968 518
Fragments arranged by size:
968 518 206
Bsu36I CC'TnA_GG
Cuts at: 0 661 1692
Size: 661 1031
BtgI C'CryG_G
Cuts at: 0 950 962 1692
Size: 950 12 730
Fragments arranged by size:
950 730 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 984 1692
Size: 984 708
BtsI GCAGTG_nn'
Cuts at: 0 135 1207 1518 1560 1692
Size: 135 1072 311 42 132
Fragments arranged by size:
1072 311 135 132 42
DraIII CAC_nnn'GTG
Cuts at: 0 581 1692
Size: 581 1111
EaeI y'GGCC_r
Cuts at: 0 2 1692
Size: 2 1690
EarI CTCTTCn'nnn_
Cuts at: 0 1672 1692
Size: 1672 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 105 416 649 829 1309 1354 1426
Size: 81 24 311 233 180 480 45 72
Cuts at: 1426 1464 1653 1692
Size: 38 189 39
Fragments arranged by size:
480 311 233 189 180 81 72 45
39 38 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 592 736 1303 1671 1692
Size: 592 144 567 368 21
Fragments arranged by size:
592 567 368 144 21
EcoO109I rG'GnC_Cy
Cuts at: 0 369 370 729 741 1317 1692
Size: 369 1 359 12 576 375
Fragments arranged by size:
576 375 369 359 12 1
FspI TGC'GCA
Cuts at: 0 1123 1692
Size: 1123 569
HaeII r_GCGC'y
Cuts at: 0 287 1692
Size: 287 1405
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1042 1074 1166 1198 1692
Size: 1042 32 92 32 494
Fragments arranged by size:
1042 494 92 32 32
HincII GTy'rAC
Cuts at: 0 420 1452 1692
Size: 420 1032 240
Fragments arranged by size:
1032 420 240
KasI G'GCGC_C
Cuts at: 0 283 1692
Size: 283 1409
KpnI G_GTAC'C
Cuts at: 0 1068 1692
Size: 1068 624
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1422 1548 1605 1692
Size: 1422 126 57 87
Fragments arranged by size:
1422 126 87 57
MscI TGG'CCA
Cuts at: 0 4 1692
Size: 4 1688
MslI CAynn'nnrTG
Cuts at: 0 109 949 1692
Size: 109 840 743
Fragments arranged by size:
840 743 109
MspA1I CmG'CkG
Cuts at: 0 227 471 672 1502 1592 1692
Size: 227 244 201 830 90 100
Fragments arranged by size:
830 244 227 201 100 90
NaeI GCC'GGC
Cuts at: 0 318 348 1692
Size: 318 30 1344
Fragments arranged by size:
1344 318 30
NarI GG'CG_CC
Cuts at: 0 284 1692
Size: 284 1408
NcoI C'CATG_G
Cuts at: 0 962 1692
Size: 962 730
NgoMIV G'CCGG_C
Cuts at: 0 316 346 1692
Size: 316 30 1346
Fragments arranged by size:
1346 316 30
NspI r_CATG'y
Cuts at: 0 152 726 1692
Size: 152 574 966
Fragments arranged by size:
966 574 152
PasI CC'CwG_GG
Cuts at: 0 76 1692
Size: 76 1616
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 1692
Size: 622 32 1038
Fragments arranged by size:
1038 622 32
PpuMI rG'GwC_Cy
Cuts at: 0 729 741 1317 1692
Size: 729 12 576 375
Fragments arranged by size:
729 576 375 12
PspOMI G'GGCC_C
Cuts at: 0 369 1692
Size: 369 1323
PstI C_TGCA'G
Cuts at: 0 596 1091 1304 1692
Size: 596 495 213 388
Fragments arranged by size:
596 495 388 213
PvuII CAG'CTG
Cuts at: 0 227 672 1692
Size: 227 445 1020
Fragments arranged by size:
1020 445 227
SapI GCTCTTCn'nnn_
Cuts at: 0 1672 1692
Size: 1672 20
SexAI A'CCwGG_T
Cuts at: 0 731 1692
Size: 731 961
SfcI C'TryA_G
Cuts at: 0 592 1087 1213 1300 1460 1692
Size: 592 495 126 87 160 232
Fragments arranged by size:
592 495 232 160 126 87
SfoI GGC'GCC
Cuts at: 0 285 1692
Size: 285 1407
SmlI C'TyrA_G
Cuts at: 0 745 1258 1692
Size: 745 513 434
Fragments arranged by size:
745 513 434
StuI AGG'CCT
Cuts at: 0 1523 1692
Size: 1523 169
StyI C'CwwG_G
Cuts at: 0 857 962 1692
Size: 857 105 730
Fragments arranged by size:
857 730 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 130 325 1516 1692
Size: 130 195 1191 176
Fragments arranged by size:
1191 195 176 130
TatI w'GTAC_w
Cuts at: 0 647 1649 1692
Size: 647 1002 43
Fragments arranged by size:
1002 647 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 565 597 1692
Size: 565 32 1095
Fragments arranged by size:
1095 565 32
Tth111I GACn'n_nGTC
Cuts at: 0 1189 1692
Size: 1189 503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1317 1692
Size: 1317 375
Enzymes that do cut:
Acc65I AcuI AleI AlfI AloI AlwNI ApaI ApoI
BanI BanII BbeI BbvCI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI
BsgI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PpiI PpuMI PspOMI PstI PvuII SapI
SexAI SfcI SfoI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbsI BcgI BciVI BclI BfrBI BmtI BplI
BsaI BsaAI BsaBI BsaWI BseYI BsiEI BsiHKAI BsiWI
BsmI BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I ClaI CspCI DraI DrdI EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspAI HindIII
HpaI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XhoI XmaI XmnI
ZraI