################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaLI AscI Asp718I AspEI AspHI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BglII BlnI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MaeII MflI MluI MluNI MroI MscI MspA1I MspI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Van91I XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 778 GTAGAC GT^[AC][GT]AC AccII 58 CGCG CG^CG AflII 648 CTTAAG C^TTAAG AocI 486 CCTGAGG CC^T[ACGT]AGG AseI 1024 ATTAAT AT^TAAT AsnI 1024 ATTAAT AT^TAAT AspI 781 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BclI 1443 TGATCA T^GATCA BfrI 648 CTTAAG C^TTAAG BlpI 876 GCTCAGC GC^T[ACGT]AGC BpmI 1375 CTGGAG CTGGAG(16/14) Bpu1102I 876 GCTCAGC GC^T[ACGT]AGC BsaJI 252 CCAAGG C^C[ACGT][ACGT]GG BsmAI 67 GAGAC GTCTC(1/5) BspHI 1401 TCATGA T^CATGA BspMI 1077 GCAGGT ACCTGC(4/8) BssKI 885 CCAGG ^CC[ACGT]GG BstF5I 1009 CATCC GGATG(2/0) BstNI 885 CCAGG CC^[AT]GG BstUI 58 CGCG CG^CG Bsu36I 486 CCTGAGG CC^T[ACGT]AGG CelII 876 GCTCAGC GC^T[ACGT]AGC EarI 1455 CTCTTC CTCTTC(1/4) Eco81I 486 CCTGAGG CC^T[ACGT]AGG EcoRI 257 GAATTC G^AATTC EcoRII 885 CCAGG ^CC[AT]GG EcoRV 163 GATATC GAT^ATC EcoT14I 252 CCAAGG C^C[AT][AT]GG FbaI 1443 TGATCA T^GATCA FokI 1009 CATCC GGATG(9/13) HincII 673 GTCAAC GT[CT]^[GA]AC HindII 673 GTCAAC GT[CT]^[GA]AC HindIII 289 AAGCTT A^AGCTT Ksp632I 1455 CTCTTC CTCTTC(1/4) MfeI 317 CAATTG C^AATTG MunI 317 CAATTG C^AATTG MvaI 885 CCAGG CC^[AT]GG MvnI 58 CGCG CG^CG PflFI 781 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 1024 ATTAAT AT^TAAT PstI 1061 CTGCAG CTGCA^G RcaI 1401 TCATGA T^CATGA ScaI 1290 AGTACT AGT^ACT ScrFI 885 CCAGG CC^[ACGT]GG SfcI 1061 CTGCAG C^T[GA][CT]AG SspI 342 AATATT AAT^ATT StyI 252 CCAAGG C^C[AT][AT]GG TfiI 726 GATTC G^A[AT]TC Tth111I 781 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 216 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 39 GAATTT [GA]^AATT[CT] 257 GAATTC ApaI 562 GGGCCC GGGCC^C 791 GGGCCC ApoI 39 GAATTT [GA]^AATT[CT] 257 GAATTC Asp700I 712 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1387 GAACAACTTC BanII 562 GGGCCC G[GA]GC[CT]^C 791 GGGCCC BmyI 562 GGGCCC G[AGT]GC[ACT]^C 791 GGGCCC BsgI 908 CTGCAC GTGCAG(16/14) 269 GTGCAG BsiYI 57 CCGCGACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1543 CCAACAGAAGG BslI 57 CCGCGACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1543 CCAACAGAAGG Bsp1286I 562 GGGCCC G[AGT]GC[ACT]^C 791 GGGCCC BsrDI 423 CATTGC GCAATG(2/0) 818 GCAATG Cac8I 525 GCAAGC GC[ACGT]^[ACGT]GC 814 GCTGGC DraII 562 GGGCCCC [GA]G^G[ACGT]CC[CT] 791 GGGCCCT EcoO109I 562 GGGCCCC [GA]G^G[ACGT]CC[CT] 791 GGGCCCT PleI 175 GACTC GAGTC(4/5) 844 GACTC TaqI 306 TCGA T^CGA 1295 TCGA TthHB8I 306 TCGA T^CGA 1295 TCGA XmnI 712 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1387 GAACAACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AfaI AluI BbsI BbvI BfaI BpuAI BsaBI BsrI Cfr13I DdeI DpnII Fnu4HI HaeIII HinfI HphI ItaI MaeI MaeIII MamI MboI MboII MnlI MseI MslI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I SfaNI SmlI Tru9I TseI Tsp509I TspRI