(Circular) (Six-base) MAPSORT of: pFN21AE4496.seq Check: 5713 from: 1 to: 6268 pFN21AE4496 6268 bp With 182 enzymes: SgfI * March 28, 2012 13:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4078 278 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2784 3611 1937 Size: 847 827 4594 Fragments arranged by size: 4594 847 827 Acc65I G'GTAC_C Cuts at: 3589 3589 Size: 6268 AclI AA'CG_TT Cuts at: 1563 4395 4768 1563 Size: 2832 373 3063 Fragments arranged by size: 3063 2832 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3351 3447 4331 5715 5983 1632 Size: 1719 96 884 1384 268 1917 Fragments arranged by size: 1917 1719 1384 884 268 96 AflII C'TTAA_G Cuts at: 820 1017 2653 820 Size: 197 1636 4435 Fragments arranged by size: 4435 1636 197 AflIII A'CryG_T Cuts at: 1203 5167 6144 1203 Size: 3964 977 1327 Fragments arranged by size: 3964 1327 977 AgeI A'CCGG_T Cuts at: 5099 5099 Size: 6268 AhdI GACnn_n'nnGTC Cuts at: 4997 4997 Size: 6268 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6268 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6268 AlwNI CAG_nnn'CTG Cuts at: 4011 5583 4011 Size: 1572 4696 ApaI G_GGCC'C Cuts at: 2571 2800 2571 Size: 229 6039 ApaLI G'TGCA_C Cuts at: 4325 5481 4325 Size: 1156 5112 ApoI r'AATT_y Cuts at: 1493 2044 2262 3577 3803 3955 6064 1493 Size: 551 218 1315 226 152 2109 1697 Fragments arranged by size: 2109 1697 1315 551 226 218 152 AseI AT'TA_AT Cuts at: 160 1032 3030 4822 160 Size: 872 1998 1792 1606 Fragments arranged by size: 1998 1792 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 AvaI C'yCGr_G Cuts at: 1870 1943 3593 1870 Size: 73 1650 4545 Fragments arranged by size: 4545 1650 73 BamHI G'GATC_C Cuts at: 3598 3965 3598 Size: 367 5901 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3589 5044 618 Size: 325 217 627 1802 1455 1842 Fragments arranged by size: 1842 1802 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2571 2800 3587 1391 Size: 1180 229 787 4072 Fragments arranged by size: 4072 1180 787 229 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2089 2184 3423 3486 928 Size: 971 190 95 1239 63 3710 Fragments arranged by size: 3710 1239 971 190 95 63 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4459 4493 1903 Size: 34 2522 34 3678 Fragments arranged by size: 3678 2522 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4160 5370 4160 Size: 1210 5058 BclI T'GATC_A Cuts at: 1548 3448 1548 Size: 1900 4368 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4879 136 Size: 107 122 71 631 605 3207 1525 Fragments arranged by size: 3207 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6263 6263 Size: 6268 BlpI GC'TnA_GC Cuts at: 2882 3685 2882 Size: 803 5465 Bme1580I G_kGCm'C Cuts at: 1790 2571 2800 4329 5485 1790 Size: 781 229 1529 1156 2573 Fragments arranged by size: 2573 1529 1156 781 229 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6268 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2566 3999 4957 Size: 416 53 83 399 24 1149 1433 958 Cuts at: 4957 442 Size: 1753 Fragments arranged by size: 1753 1433 1149 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6268 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3401 4928 1239 Size: 2162 1527 2579 Fragments arranged by size: 2579 2162 1527 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2024 2439 4393 5258 5556 2024 Size: 415 1954 865 298 2736 Fragments arranged by size: 2736 1954 865 415 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4931 882 Size: 617 3432 2219 Fragments arranged by size: 3432 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4059 493 Size: 711 2855 2702 Fragments arranged by size: 2855 2702 711 BsaBI GATnn'nnATC Cuts at: 2133 2313 2385 3105 6262 2133 Size: 180 72 720 3157 2139 Fragments arranged by size: 3157 2139 720 180 72 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4075 4457 5092 275 Size: 53 83 186 936 2542 382 635 1451 Fragments arranged by size: 2542 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4701 5099 5373 5520 5931 4701 Size: 398 274 147 411 5038 Fragments arranged by size: 5038 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6043 Fragments arranged by size: 6043 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6268 BseYI C'CCAG_C Cuts at: 2998 5471 5967 2998 Size: 2473 496 3299 Fragments arranged by size: 3299 2473 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2295 2898 1163 Size: 573 559 603 4533 Fragments arranged by size: 4533 603 573 559 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3718 4479 5507 1540 Size: 275 186 1717 761 1028 2301 Fragments arranged by size: 2301 1717 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4329 4414 5485 4329 Size: 85 1071 5112 Fragments arranged by size: 5112 1071 85 BsmI GAATG_Cn' Cuts at: 3779 3872 3779 Size: 93 6175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2571 2800 3587 4329 4414 5485 1391 Size: 399 781 229 787 742 85 1071 2174 Fragments arranged by size: 2174 1071 787 781 742 399 229 85 BspEI T'CCGG_A Cuts at: 5931 5931 Size: 6268 BspHI T'CATG_A Cuts at: 3406 4157 5887 3406 Size: 751 1730 3787 Fragments arranged by size: 3787 1730 751 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3073 3623 844 Size: 2229 550 3489 Fragments arranged by size: 3489 2229 550 BsrBI CCG'CTC Cuts at: 1394 1823 4155 1394 Size: 429 2332 3507 Fragments arranged by size: 3507 2332 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2427 2830 4763 4937 65 Size: 1183 1179 403 1933 174 1396 Fragments arranged by size: 1933 1396 1183 1179 403 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4912 5099 1700 Size: 110 3102 187 2869 Fragments arranged by size: 3102 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6268 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6268 BssSI C'ACGA_G Cuts at: 4328 5340 4328 Size: 1012 5256 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6268 BstBI TT'CG_AA Cuts at: 5072 5072 Size: 6268 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6268 BstYI r'GATC_y Cuts at: 1972 3598 3965 4350 4367 5819 5905 5917 6263 Size: 1626 367 385 17 1452 86 12 346 Cuts at: 6263 1972 Size: 1977 Fragments arranged by size: 1977 1626 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2492 2492 Size: 6268 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4777 Fragments arranged by size: 4777 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5529 Fragments arranged by size: 5529 683 56 BtsI GCAGTG_nn' Cuts at: 2541 3048 3063 3784 4578 4598 2541 Size: 507 15 721 794 20 4211 Fragments arranged by size: 4211 794 721 507 20 15 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6268 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2519 2554 529 Size: 35 1955 35 4243 Fragments arranged by size: 4243 1955 35 35 DraI TTT'AAA Cuts at: 3572 3572 Size: 6268 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5275 809 Size: 507 3959 1802 Fragments arranged by size: 3959 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3715 4604 6233 Size: 54 1425 50 275 18 1885 889 1629 Cuts at: 6233 8 Size: 43 Fragments arranged by size: 1885 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3715 1537 Size: 275 1903 4090 Fragments arranged by size: 4090 1903 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3466 4198 1353 Size: 2113 732 3423 Fragments arranged by size: 3423 2113 732 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 3528 4855 5229 5375 387 Size: 1064 138 435 1504 1327 374 146 1280 Fragments arranged by size: 1504 1327 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3351 3401 3447 4331 4928 5715 5983 Size: 393 1719 50 46 884 597 787 268 Cuts at: 5983 1239 Size: 1524 Fragments arranged by size: 1719 1524 884 787 597 393 268 50 46 EcoO109I rG'GnC_Cy Cuts at: 2568 2797 2568 Size: 229 6039 EcoRI G'AATT_C Cuts at: 2262 3577 3955 2262 Size: 1315 378 4575 Fragments arranged by size: 4575 1315 378 EcoRV GAT'ATC Cuts at: 2170 2170 Size: 6268 FspI TGC'GCA Cuts at: 4774 4774 Size: 6268 HaeII r_GCGC'y Cuts at: 1678 5415 1678 Size: 3737 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2571 2603 3433 3465 4909 Size: 32 135 32 999 32 830 32 1444 Cuts at: 4909 4941 4983 5015 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1444 999 830 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2680 3612 3858 669 Size: 988 281 742 932 246 3079 Fragments arranged by size: 3079 988 932 742 281 246 HindIII A'AGCT_T Cuts at: 748 2294 748 Size: 1546 4722 HpaI GTT'AAC Cuts at: 3858 3858 Size: 6268 KpnI G_GTAC'C Cuts at: 3593 3593 Size: 6268 MfeI C'AATT_G Cuts at: 2322 3867 2322 Size: 1545 4723 MluI A'CGCG_T Cuts at: 6144 6144 Size: 6268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3081 3253 5382 5566 1305 Size: 1776 172 2129 184 2007 Fragments arranged by size: 2129 2007 1776 184 172 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4446 Fragments arranged by size: 4446 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2517 2766 3411 4226 4585 4744 518 Size: 648 1351 249 645 815 359 159 2042 Fragments arranged by size: 2042 1351 815 648 645 359 249 159 MspA1I CmG'CkG Cuts at: 1606 3684 4361 5509 5754 1606 Size: 2078 677 1148 245 2120 Fragments arranged by size: 2120 2078 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4777 Fragments arranged by size: 4777 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6268 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6268 NotI GC'GGCC_GC Cuts at: 3715 3715 Size: 6268 NspI r_CATG'y Cuts at: 1132 1229 3626 5171 1132 Size: 97 2397 1545 2229 Fragments arranged by size: 2397 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6268 PciI A'CATG_T Cuts at: 5167 5167 Size: 6268 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6268 PmeI GTTT'AAAC Cuts at: 3572 3572 Size: 6268 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4337 4369 5850 5882 4337 Size: 32 1481 32 4723 Fragments arranged by size: 4723 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6268 PsiI TTA'TAA Cuts at: 3838 3838 Size: 6268 PspOMI G'GGCC_C Cuts at: 2567 2796 2567 Size: 229 6039 PstI C_TGCA'G Cuts at: 830 3070 3620 830 Size: 2240 550 3478 Fragments arranged by size: 3478 2240 550 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6268 SalI G'TCGA_C Cuts at: 1936 3610 1936 Size: 1674 4594 SbfI CC_TGCA'GG Cuts at: 3620 3620 Size: 6268 ScaI AGT'ACT Cuts at: 3297 4516 6228 3297 Size: 1219 1712 3337 Fragments arranged by size: 3337 1712 1219 SfcI C'TryA_G Cuts at: 826 1045 3066 3616 4751 5432 5623 826 Size: 219 2021 550 1135 681 191 1471 Fragments arranged by size: 2021 1471 1135 681 550 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6268 SmaI CCC'GGG Cuts at: 1872 3595 1872 Size: 1723 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2039 2454 2653 4372 5273 5535 Size: 197 926 96 415 199 1719 901 262 Cuts at: 5535 820 Size: 1553 Fragments arranged by size: 1719 1553 926 901 415 262 199 197 96 SnaBI TAC'GTA Cuts at: 493 4059 493 Size: 3566 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6268 SphI G_CATG'C Cuts at: 1132 3626 1132 Size: 2494 3774 SspI AAT'ATT Cuts at: 5 52 2349 4192 5 Size: 47 2297 1843 2081 Fragments arranged by size: 2297 2081 1843 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6268 StyI C'CwwG_G Cuts at: 513 1065 2004 2257 3707 513 Size: 552 939 253 1450 3074 Fragments arranged by size: 3074 1450 939 552 253 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4295 4312 4465 4650 5095 325 Size: 1255 2715 17 153 185 445 1498 Fragments arranged by size: 2715 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3295 4514 6226 Size: 274 80 33 51 1443 1318 1219 1712 Cuts at: 6226 96 Size: 138 Fragments arranged by size: 1712 1443 1318 1219 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6228 6260 6228 Size: 32 6236 Tth111I GACn'n_nGTC Cuts at: 1316 2789 1316 Size: 1473 4795 XbaI T'CTAG_A Cuts at: 2221 3604 2221 Size: 1383 4885 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6268 XmaI C'CCGG_G Cuts at: 1870 3593 1870 Size: 1723 4545 XmnI GAAnn'nnTTC Cuts at: 2721 3396 4397 2721 Size: 675 1001 4592 Fragments arranged by size: 4592 1001 675 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4076 276 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI