################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccI AccIII AflIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvrII BbrPI BbvCI BcgI BciVI BclI BcnI BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmI BspEI BspHI BspLU11I BssHII Bst1107I BstBI BstEII BstPI BstZ17I CpoI DraI DraIII DrdI Eco47III EcoO65I EcoRI FbaI FseI HincII HindII HindIII HpaI KpnI MamI MluI MroI NciI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SalI SbfI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AclI 117 AACGTT AA^CGTT AcsI 574 AAATTC [GA]^AATT[CT] AflII 301 CTTAAG C^TTAAG AhdI 2320 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1220 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1971 GGGCCC GGGCC^C ApoI 574 AAATTC [GA]^AATT[CT] AspEI 2320 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2102 TGCGCA TGC^GCA BalI 1385 TGGCCA TGG^CCA BamHI 1298 GGATCC G^GATCC BanI 9 GGCGCC G^G[CT][GA]CC BbeI 9 GGCGCC GGCGC^C BfrI 301 CTTAAG C^TTAAG BglII 2216 AGATCT A^GATCT BsiEI 984 CGGCCG CG[GA][CT]^CG Bsp1407I 971 TGTACA T^GTACA BspDI 2458 ATCGAT AT^CGAT BsrDI 1044 CATTGC GCAATG(2/0) BsrGI 971 TGTACA T^GTACA BssSI 60 CACGAG CACGAG(-5/-1) BstAPI 826 GCAGCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 2458 ATCGAT AT^CGAT EagI 984 CGGCCG C^GGCCG Eam1105I 2320 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 984 CGGCCG C^GGCCG Eco52I 984 CGGCCG C^GGCCG EcoNI 402 CCTGATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1549 GATATC GAT^ATC EcoT22I 899 ATGCAT ATGCA^T FspI 2102 TGCGCA TGC^GCA KasI 9 GGCGCC G^GCGCC KspI 360 CCGCGG CCGC^GG MaeII 118 ACGT A^CGT MfeI 2415 CAATTG C^AATTG MluNI 1385 TGGCCA TGG^CCA MscI 1385 TGGCCA TGG^CCA MunI 2415 CAATTG C^AATTG NarI 9 GGCGCC GG^CGCC NcoI 2034 CCATGG C^CATGG NsiI 899 ATGCAT ATGCA^T NspI 1370 GCATGC [GA]CATG^[CT] PpuMI 1005 AGGACCC [GA]G^G[AT]CC[CT] Psp1406I 117 AACGTT AA^CGTT SacII 360 CCGCGG CCGC^GG SapI 127 GAAGAGC GCTCTTC(1/4) SfiI 2179 GGCCGCTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 9 GGCGCC GGC^GCC SphI 1370 GCATGC GCATG^C SspBI 971 TGTACA T^GTACA XcmI 1433 CCACCAACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccII 361 CGCG CG^CG 2246 CGCG AcyI 9 GGCGCC G[GA]^CG[CT]C 2115 GACGCC AocI 496 CCTGAGG CC^T[ACGT]AGG 1813 CCTAAGG AvaI 34 CCCGAG C^[CT]CG[GA]G 2528 CCCGAG AvaII 1006 GGACC G^G[AT]CC 1446 GGACC BbsI 1494 GAAGAC GAAGAC(2/6) 2473 GAAGAC BfaI 1234 CTAG C^TAG 2341 CTAG BlpI 559 GCTAAGC GC^T[ACGT]AGC 2260 GCTGAGC Bpu1102I 559 GCTAAGC GC^T[ACGT]AGC 2260 GCTGAGC BpuAI 1494 GAAGAC GAAGAC(2/6) 2473 GAAGAC BsaHI 9 GGCGCC G[GA]^CG[CT]C 2115 GACGCC BsgI 512 CTGCAC GTGCAG(16/14) 1542 CTGCAC BsmBI 1509 CGTCTC CGTCTC(1/5) 2113 GAGACG BsoBI 34 CCCGAG C^[CT]CG[GA]G 2528 CCCGAG BspMI 2129 ACCTGC ACCTGC(4/8) 2147 ACCTGC BsrBI 935 CCGCTC CCGCTC(-3/-3) 1180 GAGCGG BstUI 361 CGCG CG^CG 2246 CGCG BstYI 1298 GGATCC [GA]^GATC[CT] 2216 AGATCT Bsu36I 496 CCTGAGG CC^T[ACGT]AGG 1813 CCTAAGG CelII 559 GCTAAGC GC^T[ACGT]AGC 2260 GCTGAGC DsaI 360 CCGCGG C^C[GA][CT]GG 2034 CCATGG EaeI 984 CGGCCG [CT]^GGCC[GA] 1385 TGGCCA Eco81I 496 CCTGAGG CC^T[ACGT]AGG 1813 CCTAAGG EcoT14I 377 CCAAGG C^C[AT][AT]GG 2034 CCATGG HgaI 2115 GACGC GACGC(5/10) 297 GCGTC Hin1I 9 GGCGCC G[GA]^CG[CT]C 2115 GACGCC MaeI 1234 CTAG C^TAG 2341 CTAG MflI 1298 GGATCC [GA]^GATC[CT] 2216 AGATCT MvnI 361 CGCG CG^CG 2246 CGCG NaeI 982 GCCGGC GCC^GGC 2522 GCCGGC NgoMIV 982 GCCGGC G^CCGGC 2522 GCCGGC PstI 1277 CTGCAG CTGCA^G 2131 CTGCAG SacI 286 GAGCTC GAGCT^C 1798 GAGCTC StyI 377 CCAAGG C^C[AT][AT]GG 2034 CCATGG XhoII 1298 GGATCC [GA]^GATC[CT] 2216 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI BanII BbvI BglI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I