(Circular) (Six-base) MAPSORT of: pFN21AE5710.seq Check: 3138 from: 1 to: 7240 pFN21AE5710 7240 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5050 278 Size: 53 83 186 936 3514 2468 Fragments arranged by size: 3514 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4583 1937 Size: 2646 4594 Acc65I G'GTAC_C Cuts at: 4561 4561 Size: 7240 AclI AA'CG_TT Cuts at: 1563 2123 5367 5740 1563 Size: 560 3244 373 3063 Fragments arranged by size: 3244 3063 560 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3179 3698 3707 3830 5303 6687 6955 1632 Size: 1547 519 9 123 1473 1384 268 1917 Fragments arranged by size: 1917 1547 1473 1384 519 268 123 9 AflII C'TTAA_G Cuts at: 820 1017 2306 820 Size: 197 1289 5754 Fragments arranged by size: 5754 1289 197 AflIII A'CryG_T Cuts at: 1203 6139 7116 1203 Size: 4936 977 1327 Fragments arranged by size: 4936 1327 977 AgeI A'CCGG_T Cuts at: 6071 6071 Size: 7240 AhdI GACnn_n'nnGTC Cuts at: 4330 5969 4330 Size: 1639 5601 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7240 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7240 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4275 4307 4275 Size: 32 7208 AlwNI CAG_nnn'CTG Cuts at: 3230 4983 6555 3230 Size: 1753 1572 3915 Fragments arranged by size: 3915 1753 1572 ApaI G_GGCC'C Cuts at: 3980 3980 Size: 7240 ApaLI G'TGCA_C Cuts at: 5297 6453 5297 Size: 1156 6084 ApoI r'AATT_y Cuts at: 1493 2579 4549 4775 4927 7036 1493 Size: 1086 1970 226 152 2109 1697 Fragments arranged by size: 2109 1970 1697 1086 226 152 AseI AT'TA_AT Cuts at: 160 1032 5794 160 Size: 872 4762 1606 Fragments arranged by size: 4762 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7240 AvaI C'yCGr_G Cuts at: 1870 1943 2039 4533 4565 1870 Size: 73 96 2494 32 4545 Fragments arranged by size: 4545 2494 96 73 32 BamHI G'GATC_C Cuts at: 3303 4570 4937 3303 Size: 1267 367 5606 Fragments arranged by size: 5606 1267 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 4561 6016 618 Size: 325 217 627 227 2547 1455 1842 Fragments arranged by size: 2547 1842 1455 627 325 227 217 BanII G_rGCy'C Cuts at: 1391 2040 2072 2295 3064 3414 3438 3807 3980 Size: 649 32 223 769 350 24 369 173 Cuts at: 3980 4559 1391 Size: 579 4072 Fragments arranged by size: 4072 769 649 579 369 350 223 173 32 24 BbeI G_GCGC'C Cuts at: 2018 2018 Size: 7240 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3506 4485 928 Size: 971 1607 979 3683 Fragments arranged by size: 3683 1607 979 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5431 5465 1903 Size: 34 50 34 3410 34 3678 Fragments arranged by size: 3678 3410 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5132 6342 5132 Size: 1210 6030 BclI T'GATC_A Cuts at: 1548 1548 Size: 7240 BfrBI ATG'CAT Cuts at: 2906 2906 Size: 7240 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2672 3272 4191 Size: 107 122 71 631 605 1000 600 919 Cuts at: 4191 5851 136 Size: 1660 1525 Fragments arranged by size: 1660 1525 1000 919 631 605 600 122 107 71 BglII A'GATC_T Cuts at: 4221 7235 4221 Size: 3014 4226 BlpI GC'TnA_GC Cuts at: 2565 4266 4657 2565 Size: 1701 391 5148 Fragments arranged by size: 5148 1701 391 Bme1580I G_kGCm'C Cuts at: 1790 3400 3980 5301 6457 1790 Size: 1610 580 1321 1156 2573 Fragments arranged by size: 2573 1610 1321 1156 580 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7240 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3279 4971 5929 Size: 416 53 83 399 24 1862 1692 958 Cuts at: 5929 442 Size: 1753 Fragments arranged by size: 1862 1753 1692 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7240 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2896 3090 2896 Size: 194 7046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2942 3470 3855 4217 5900 1239 Size: 1703 528 385 362 1683 2579 Fragments arranged by size: 2579 1703 1683 528 385 362 Bpu10I CC'TnA_GC Cuts at: 2457 3065 3801 2457 Size: 608 736 5896 Fragments arranged by size: 5896 736 608 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2165 2507 3026 5365 6230 6528 2165 Size: 342 519 2339 865 298 2877 Fragments arranged by size: 2877 2339 865 519 342 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5903 882 Size: 617 4404 2219 Fragments arranged by size: 4404 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5031 493 Size: 711 3827 2702 Fragments arranged by size: 3827 2702 711 BsaBI GATnn'nnATC Cuts at: 7234 7234 Size: 7240 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 4121 5047 5429 Size: 53 83 186 936 482 2106 926 382 Cuts at: 5429 6064 275 Size: 635 1451 Fragments arranged by size: 2106 1451 936 926 635 482 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5673 6071 6345 6492 6903 5673 Size: 398 274 147 411 6010 Fragments arranged by size: 6010 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 1178 Size: 30 165 30 624 30 6361 Fragments arranged by size: 6361 624 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2351 2447 2450 2618 2729 2732 2742 3029 Size: 1165 96 3 168 111 3 10 287 Cuts at: 3029 3197 4130 4457 1186 Size: 168 933 327 3969 Fragments arranged by size: 3969 1165 933 327 287 168 168 111 96 10 3 3 BseYI C'CCAG_C Cuts at: 2159 3573 3877 6443 6939 2159 Size: 1414 304 2566 496 2460 Fragments arranged by size: 2566 2460 1414 496 304 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2502 3532 1163 Size: 573 766 1030 4871 Fragments arranged by size: 4871 1030 766 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2992 4690 5451 6479 1540 Size: 275 186 991 1698 761 1028 2301 Fragments arranged by size: 2301 1698 1028 991 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2066 2181 2295 3807 5301 5386 6457 2066 Size: 115 114 1512 1494 85 1071 2849 Fragments arranged by size: 2849 1512 1494 1071 115 114 85 BsmI GAATG_Cn' Cuts at: 4751 4844 4751 Size: 93 7147 BsmBI CGTCTCn'nnnn_ Cuts at: 3520 4112 3520 Size: 592 6648 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2066 2072 2181 2295 3064 3400 Size: 399 250 26 6 109 114 769 336 Cuts at: 3400 3414 3438 3807 3980 4559 5301 5386 6457 Size: 14 24 369 173 579 742 85 1071 Cuts at: 6457 1391 Size: 2174 Fragments arranged by size: 2174 1071 769 742 579 399 369 336 250 173 114 109 85 26 24 14 6 BspEI T'CCGG_A Cuts at: 6903 6903 Size: 7240 BspHI T'CATG_A Cuts at: 5129 6859 5129 Size: 1730 5510 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4143 4161 4595 844 Size: 3299 18 434 3489 Fragments arranged by size: 3489 3299 434 18 BsrBI CCG'CTC Cuts at: 1394 1823 2942 3187 5127 1394 Size: 429 1119 245 1940 3507 Fragments arranged by size: 3507 1940 1119 429 245 BsrDI GCAATG_nn' Cuts at: 65 1248 3048 5735 5909 65 Size: 1183 1800 2687 174 1396 Fragments arranged by size: 2687 1800 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2987 4062 4527 5884 6071 1700 Size: 110 1177 1075 465 1357 187 2869 Fragments arranged by size: 2869 1357 1177 1075 465 187 110 BsrGI T'GTAC_A Cuts at: 96 2976 96 Size: 2880 4360 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7240 BssSI C'ACGA_G Cuts at: 2065 5300 6312 2065 Size: 3235 1012 2993 Fragments arranged by size: 3235 2993 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2837 794 Size: 2043 5197 BstBI TT'CG_AA Cuts at: 6044 6044 Size: 7240 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7240 BstXI CCAn_nnnn'nTGG Cuts at: 3448 4321 4466 3448 Size: 873 145 6222 Fragments arranged by size: 6222 873 145 BstYI r'GATC_y Cuts at: 1972 3303 4221 4570 4937 5322 5339 6791 6877 Size: 1331 918 349 367 385 17 1452 86 Cuts at: 6877 6889 7235 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1331 918 385 367 349 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2502 3819 2502 Size: 1317 5923 BtgI C'CryG_G Cuts at: 513 1065 2365 4039 513 Size: 552 1300 1674 3714 Fragments arranged by size: 3714 1674 1300 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6501 Fragments arranged by size: 6501 683 56 BtsI GCAGTG_nn' Cuts at: 3416 4756 5550 5570 3416 Size: 1340 794 20 5086 Fragments arranged by size: 5086 1340 794 20 ClaI AT'CG_AT Cuts at: 1554 4464 1554 Size: 2910 4330 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7205 DraI TTT'AAA Cuts at: 4544 4544 Size: 7240 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6247 809 Size: 507 4931 1802 Fragments arranged by size: 4931 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2989 3390 4687 Size: 54 1425 50 275 18 1159 401 1297 Cuts at: 4687 5576 7205 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1297 1159 889 401 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2989 4687 1537 Size: 275 1177 1698 4090 Fragments arranged by size: 4090 1698 1177 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 2163 2277 2424 2538 2595 2706 3174 Size: 774 36 114 147 114 57 111 468 Cuts at: 3174 3989 5170 1353 Size: 815 1181 3423 Fragments arranged by size: 3423 1181 815 774 468 147 114 114 111 57 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2269 2885 3316 3511 3731 5827 Size: 1064 138 680 616 431 195 220 2096 Cuts at: 5827 6201 6347 387 Size: 374 146 1280 Fragments arranged by size: 2096 1280 1064 680 616 431 374 220 195 146 138 EcoICRI GAG'CTC Cuts at: 2293 3805 2293 Size: 1512 5728 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2942 3179 3470 3698 3707 3830 3855 Size: 393 1310 237 291 228 9 123 25 Cuts at: 3855 4217 5303 5900 6687 6955 1239 Size: 362 1086 597 787 268 1524 Fragments arranged by size: 1524 1310 1086 787 597 393 362 291 268 237 228 123 25 9 EcoNI CCTnn'n_nnAGG Cuts at: 2411 2411 Size: 7240 EcoO109I rG'GnC_Cy Cuts at: 3011 3796 3977 4504 3011 Size: 785 181 527 5747 Fragments arranged by size: 5747 785 527 181 EcoRI G'AATT_C Cuts at: 4549 4927 4549 Size: 378 6862 EcoRV GAT'ATC Cuts at: 3556 3556 Size: 7240 FspI TGC'GCA Cuts at: 4109 5746 4109 Size: 1637 5603 HaeII r_GCGC'y Cuts at: 1678 2018 2812 3817 6387 1678 Size: 340 794 1005 2570 2531 Fragments arranged by size: 2570 2531 1005 794 340 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2401 2433 3770 3802 4316 Size: 32 135 32 829 32 1337 32 514 Cuts at: 4316 4348 5881 5913 5955 5987 1373 Size: 32 1533 32 42 32 2626 Fragments arranged by size: 2626 1533 1337 829 514 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4584 4830 669 Size: 988 281 2646 246 3079 Fragments arranged by size: 3079 2646 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7240 HpaI GTT'AAC Cuts at: 4830 4830 Size: 7240 KasI G'GCGC_C Cuts at: 2014 2014 Size: 7240 KpnI G_GTAC'C Cuts at: 4565 4565 Size: 7240 MfeI C'AATT_G Cuts at: 4420 4839 4420 Size: 419 6821 MluI A'CGCG_T Cuts at: 7116 7116 Size: 7240 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4479 6354 6538 1305 Size: 3174 1875 184 2007 Fragments arranged by size: 3174 2007 1875 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3392 10 Size: 54 1425 343 1560 3858 Fragments arranged by size: 3858 1560 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3086 3567 3950 5198 5557 5716 518 Size: 648 1920 481 383 1248 359 159 2042 Fragments arranged by size: 2042 1920 1248 648 481 383 359 159 MspA1I CmG'CkG Cuts at: 1606 2367 2843 3205 3227 3281 3577 4105 4141 Size: 761 476 362 22 54 296 528 36 Cuts at: 4141 4429 4656 5333 6481 6726 1606 Size: 288 227 677 1148 245 2120 Fragments arranged by size: 2120 1148 761 677 528 476 362 296 288 245 227 54 36 22 NaeI GCC'GGC Cuts at: 2989 4529 2989 Size: 1540 5700 NarI GG'CG_CC Cuts at: 2015 2015 Size: 7240 NcoI C'CATG_G Cuts at: 513 1065 4039 513 Size: 552 2974 3714 Fragments arranged by size: 3714 2974 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7240 NgoMIV G'CCGG_C Cuts at: 2987 4527 2987 Size: 1540 5700 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7240 NotI GC'GGCC_GC Cuts at: 4687 4687 Size: 7240 NsiI A_TGCA'T Cuts at: 2908 2908 Size: 7240 NspI r_CATG'y Cuts at: 1132 1229 3379 4598 6143 1132 Size: 97 2150 1219 1545 2229 Fragments arranged by size: 2229 2150 1545 1219 97 PasI CC'CwG_GG Cuts at: 1348 4507 1348 Size: 3159 4081 PciI A'CATG_T Cuts at: 6139 6139 Size: 7240 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7240 PmeI GTTT'AAAC Cuts at: 4544 4544 Size: 7240 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5309 5341 6822 6854 5309 Size: 32 1481 32 5695 Fragments arranged by size: 5695 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3011 3011 Size: 7240 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7240 PsiI TTA'TAA Cuts at: 2627 4810 2627 Size: 2183 5057 PspOMI G'GGCC_C Cuts at: 3976 3976 Size: 7240 PstI C_TGCA'G Cuts at: 830 3286 4140 4592 830 Size: 2456 854 452 3478 Fragments arranged by size: 3478 2456 854 452 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7240 PvuII CAG'CTG Cuts at: 2843 3205 3227 3281 4105 4429 2843 Size: 362 22 54 824 324 5654 Fragments arranged by size: 5654 824 362 324 54 22 SacI G_AGCT'C Cuts at: 2295 3807 2295 Size: 1512 5728 SacII CC_GC'GG Cuts at: 2368 2368 Size: 7240 SalI G'TCGA_C Cuts at: 1936 4582 1936 Size: 2646 4594 SapI GCTCTTCn'nnn_ Cuts at: 2127 2127 Size: 7240 SbfI CC_TGCA'GG Cuts at: 4592 4592 Size: 7240 ScaI AGT'ACT Cuts at: 5488 7200 5488 Size: 1712 5528 SfcI C'TryA_G Cuts at: 826 1045 3282 3589 4136 4588 5723 6404 6595 Size: 219 2237 307 547 452 1135 681 191 Cuts at: 6595 826 Size: 1471 Fragments arranged by size: 2237 1471 1135 681 547 452 307 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 4191 4191 Size: 7240 SfoI GGC'GCC Cuts at: 2016 2016 Size: 7240 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7240 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7240 SmaI CCC'GGG Cuts at: 1872 4567 1872 Size: 2695 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2180 2306 2522 3041 5344 6245 Size: 197 926 237 126 216 519 2303 901 Cuts at: 6245 6507 820 Size: 262 1553 Fragments arranged by size: 2303 1553 926 901 519 262 237 216 197 126 SnaBI TAC'GTA Cuts at: 493 5031 493 Size: 4538 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7240 SphI G_CATG'C Cuts at: 1132 3379 4598 1132 Size: 2247 1219 3774 Fragments arranged by size: 3774 2247 1219 SspI AAT'ATT Cuts at: 5 52 5164 5 Size: 47 5112 2081 Fragments arranged by size: 5112 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7240 StyI C'CwwG_G Cuts at: 513 1065 2382 4039 4679 513 Size: 552 1317 1657 640 3074 Fragments arranged by size: 3074 1657 1317 640 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4423 4499 5267 5284 5437 5622 6067 Size: 1255 2843 76 768 17 153 185 445 Cuts at: 6067 325 Size: 1498 Fragments arranged by size: 2843 1498 1255 768 445 185 153 76 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2976 3825 5486 Size: 274 80 33 51 1443 999 849 1661 Cuts at: 5486 7198 96 Size: 1712 138 Fragments arranged by size: 1712 1661 1443 999 849 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2027 2059 3117 3149 3288 3320 7200 7232 2027 Size: 32 1058 32 139 32 3880 32 2035 Fragments arranged by size: 3880 2035 1058 139 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7240 XbaI T'CTAG_A Cuts at: 4576 4576 Size: 7240 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3445 3445 Size: 7240 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7240 XmaI C'CCGG_G Cuts at: 1870 4565 1870 Size: 2695 4545 XmnI GAAnn'nnTTC Cuts at: 5369 5369 Size: 7240 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5048 276 Size: 53 83 186 936 3514 2468 Fragments arranged by size: 3514 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BsiWI BstZ17I DraIII FalI FseI FspAI NruI PacI PfoI PmlI PspXI PsrI RsrII SanDI SexAI SrfI SwaI