(Linear) (Six-base) MAPSORT of: KIEE5710.seq Check: 3256 from: 1 to: 2535 KIEE5710.orf With 182 enzymes: SgfI * December 5, 2014 13:07 .. AclI AA'CG_TT Cuts at: 0 118 2535 Size: 118 2417 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1174 1693 1702 1825 2535 Size: 1174 519 9 123 710 Fragments arranged by size: 1174 710 519 123 9 AflII C'TTAA_G Cuts at: 0 301 2535 Size: 301 2234 AhdI GACnn_n'nnGTC Cuts at: 0 2325 2535 Size: 2325 210 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2270 2302 2535 Size: 2270 32 233 Fragments arranged by size: 2270 233 32 AlwNI CAG_nnn'CTG Cuts at: 0 1225 2535 Size: 1225 1310 ApaI G_GGCC'C Cuts at: 0 1975 2535 Size: 1975 560 ApoI r'AATT_y Cuts at: 0 574 2535 Size: 574 1961 AvaI C'yCGr_G Cuts at: 0 34 2528 2535 Size: 34 2494 7 Fragments arranged by size: 2494 34 7 BamHI G'GATC_C Cuts at: 0 1298 2535 Size: 1298 1237 BanI G'GyrC_C Cuts at: 0 9 2535 Size: 9 2526 BanII G_rGCy'C Cuts at: 0 35 67 290 1059 1409 1433 1802 1975 Size: 35 32 223 769 350 24 369 173 Cuts at: 1975 2535 Size: 560 Fragments arranged by size: 769 560 369 350 223 173 35 32 24 BbeI G_GCGC'C Cuts at: 0 13 2535 Size: 13 2522 BbsI GAAGACnn'nnnn_ Cuts at: 0 1501 2480 2535 Size: 1501 979 55 Fragments arranged by size: 1501 979 55 BfrBI ATG'CAT Cuts at: 0 901 2535 Size: 901 1634 BglI GCCn_nnn'nGGC Cuts at: 0 667 1267 2186 2535 Size: 667 600 919 349 Fragments arranged by size: 919 667 600 349 BglII A'GATC_T Cuts at: 0 2216 2535 Size: 2216 319 BlpI GC'TnA_GC Cuts at: 0 560 2261 2535 Size: 560 1701 274 Fragments arranged by size: 1701 560 274 Bme1580I G_kGCm'C Cuts at: 0 1395 1975 2535 Size: 1395 580 560 Fragments arranged by size: 1395 580 560 BmrI ACTGGGnnnn_n' Cuts at: 0 1274 2535 Size: 1274 1261 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 891 1085 2535 Size: 891 194 1450 Fragments arranged by size: 1450 891 194 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 937 1465 1850 2212 2535 Size: 937 528 385 362 323 Fragments arranged by size: 937 528 385 362 323 Bpu10I CC'TnA_GC Cuts at: 0 452 1060 1796 2535 Size: 452 608 736 739 Fragments arranged by size: 739 736 608 452 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 160 502 1021 2535 Size: 160 342 519 1514 Fragments arranged by size: 1514 519 342 160 BsaHI Gr'CG_yC Cuts at: 0 10 2116 2535 Size: 10 2106 419 Fragments arranged by size: 2106 419 10 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 22 52 2535 Size: 22 30 2483 Fragments arranged by size: 2483 30 22 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 346 442 445 613 724 727 737 1024 Size: 346 96 3 168 111 3 10 287 Cuts at: 1024 1192 2125 2452 2535 Size: 168 933 327 83 Fragments arranged by size: 933 346 327 287 168 168 111 96 83 10 3 3 BseYI C'CCAG_C Cuts at: 0 154 1568 1872 2535 Size: 154 1414 304 663 Fragments arranged by size: 1414 663 304 154 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 497 1527 2535 Size: 497 1030 1008 Fragments arranged by size: 1030 1008 497 BsiEI CG_ry'CG Cuts at: 0 987 2535 Size: 987 1548 BsiHKAI G_wGCw'C Cuts at: 0 61 176 290 1802 2535 Size: 61 115 114 1512 733 Fragments arranged by size: 1512 733 115 114 61 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1515 2107 2535 Size: 1515 592 428 Fragments arranged by size: 1515 592 428 Bsp1286I G_dGCh'C Cuts at: 0 35 61 67 176 290 1059 1395 1409 Size: 35 26 6 109 114 769 336 14 Cuts at: 1409 1433 1802 1975 2535 Size: 24 369 173 560 Fragments arranged by size: 769 560 369 336 173 114 109 35 26 24 14 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2138 2156 2535 Size: 2138 18 379 Fragments arranged by size: 2138 379 18 BsrBI CCG'CTC Cuts at: 0 937 1182 2535 Size: 937 245 1353 Fragments arranged by size: 1353 937 245 BsrDI GCAATG_nn' Cuts at: 0 1043 2535 Size: 1043 1492 BsrFI r'CCGG_y Cuts at: 0 982 2057 2522 2535 Size: 982 1075 465 13 Fragments arranged by size: 1075 982 465 13 BsrGI T'GTAC_A Cuts at: 0 971 2535 Size: 971 1564 BssSI C'ACGA_G Cuts at: 0 60 2535 Size: 60 2475 BstAPI GCAn_nnn'nTGC Cuts at: 0 832 2535 Size: 832 1703 BstXI CCAn_nnnn'nTGG Cuts at: 0 1443 2316 2461 2535 Size: 1443 873 145 74 Fragments arranged by size: 1443 873 145 74 BstYI r'GATC_y Cuts at: 0 1298 2216 2535 Size: 1298 918 319 Fragments arranged by size: 1298 918 319 Bsu36I CC'TnA_GG Cuts at: 0 497 1814 2535 Size: 497 1317 721 Fragments arranged by size: 1317 721 497 BtgI C'CryG_G Cuts at: 0 360 2034 2535 Size: 360 1674 501 Fragments arranged by size: 1674 501 360 BtsI GCAGTG_nn' Cuts at: 0 1411 2535 Size: 1411 1124 ClaI AT'CG_AT Cuts at: 0 2459 2535 Size: 2459 76 EaeI y'GGCC_r Cuts at: 0 984 1385 2535 Size: 984 401 1150 Fragments arranged by size: 1150 984 401 EagI C'GGCC_G Cuts at: 0 984 2535 Size: 984 1551 EarI CTCTTCn'nnn_ Cuts at: 0 122 158 272 419 533 590 701 1169 Size: 122 36 114 147 114 57 111 468 Cuts at: 1169 1984 2535 Size: 815 551 Fragments arranged by size: 815 551 468 147 122 114 114 111 57 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 264 880 1311 1506 1726 2535 Size: 264 616 431 195 220 809 Fragments arranged by size: 809 616 431 264 220 195 EcoICRI GAG'CTC Cuts at: 0 288 1800 2535 Size: 288 1512 735 Fragments arranged by size: 1512 735 288 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 937 1174 1465 1693 1702 1825 1850 2212 Size: 937 237 291 228 9 123 25 362 Cuts at: 2212 2535 Size: 323 Fragments arranged by size: 937 362 323 291 237 228 123 25 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 406 2535 Size: 406 2129 EcoO109I rG'GnC_Cy Cuts at: 0 1006 1791 1972 2499 2535 Size: 1006 785 181 527 36 Fragments arranged by size: 1006 785 527 181 36 EcoRV GAT'ATC Cuts at: 0 1551 2535 Size: 1551 984 FspI TGC'GCA Cuts at: 0 2104 2535 Size: 2104 431 HaeII r_GCGC'y Cuts at: 0 13 807 1812 2535 Size: 13 794 1005 723 Fragments arranged by size: 1005 794 723 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 396 428 1765 1797 2311 2343 2535 Size: 396 32 1337 32 514 32 192 Fragments arranged by size: 1337 514 396 192 32 32 32 KasI G'GCGC_C Cuts at: 0 9 2535 Size: 9 2526 MfeI C'AATT_G Cuts at: 0 2415 2535 Size: 2415 120 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2474 2535 Size: 2474 61 MscI TGG'CCA Cuts at: 0 1387 2535 Size: 1387 1148 MslI CAynn'nnrTG Cuts at: 0 1081 1562 1945 2535 Size: 1081 481 383 590 Fragments arranged by size: 1081 590 481 383 MspA1I CmG'CkG Cuts at: 0 362 838 1200 1222 1276 1572 2100 2136 Size: 362 476 362 22 54 296 528 36 Cuts at: 2136 2424 2535 Size: 288 111 Fragments arranged by size: 528 476 362 362 296 288 111 54 36 22 NaeI GCC'GGC Cuts at: 0 984 2524 2535 Size: 984 1540 11 Fragments arranged by size: 1540 984 11 NarI GG'CG_CC Cuts at: 0 10 2535 Size: 10 2525 NcoI C'CATG_G Cuts at: 0 2034 2535 Size: 2034 501 NgoMIV G'CCGG_C Cuts at: 0 982 2522 2535 Size: 982 1540 13 Fragments arranged by size: 1540 982 13 NsiI A_TGCA'T Cuts at: 0 903 2535 Size: 903 1632 NspI r_CATG'y Cuts at: 0 1374 2535 Size: 1374 1161 PasI CC'CwG_GG Cuts at: 0 2502 2535 Size: 2502 33 PpuMI rG'GwC_Cy Cuts at: 0 1006 2535 Size: 1006 1529 PsiI TTA'TAA Cuts at: 0 622 2535 Size: 622 1913 PspOMI G'GGCC_C Cuts at: 0 1971 2535 Size: 1971 564 PstI C_TGCA'G Cuts at: 0 1281 2135 2535 Size: 1281 854 400 Fragments arranged by size: 1281 854 400 PvuII CAG'CTG Cuts at: 0 838 1200 1222 1276 2100 2424 2535 Size: 838 362 22 54 824 324 111 Fragments arranged by size: 838 824 362 324 111 54 22 SacI G_AGCT'C Cuts at: 0 290 1802 2535 Size: 290 1512 733 Fragments arranged by size: 1512 733 290 SacII CC_GC'GG Cuts at: 0 363 2535 Size: 363 2172 SapI GCTCTTCn'nnn_ Cuts at: 0 122 2535 Size: 122 2413 SfcI C'TryA_G Cuts at: 0 1277 1584 2131 2535 Size: 1277 307 547 404 Fragments arranged by size: 1277 547 404 307 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2186 2535 Size: 2186 349 SfoI GGC'GCC Cuts at: 0 11 2535 Size: 11 2524 SmlI C'TyrA_G Cuts at: 0 175 301 517 1036 2535 Size: 175 126 216 519 1499 Fragments arranged by size: 1499 519 216 175 126 SphI G_CATG'C Cuts at: 0 1374 2535 Size: 1374 1161 StyI C'CwwG_G Cuts at: 0 377 2034 2535 Size: 377 1657 501 Fragments arranged by size: 1657 501 377 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2418 2494 2535 Size: 2418 76 41 Fragments arranged by size: 2418 76 41 TatI w'GTAC_w Cuts at: 0 971 1820 2535 Size: 971 849 715 Fragments arranged by size: 971 849 715 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 22 54 1112 1144 1283 1315 2535 Size: 22 32 1058 32 139 32 1220 Fragments arranged by size: 1220 1058 139 32 32 32 22 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1440 2535 Size: 1440 1095 Enzymes that do cut: AclI AcuI AflII AhdI AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI ClaI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PpuMI PsiI PspOMI PstI PvuII SacI SacII SapI SfcI SfiI SfoI SmlI SphI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflIII AgeI AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BmgBI BmtI BsaI BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI BssHII BstBI BstEII BstZ17I BtgZI CspCI DraI DraIII DrdI EcoRI FalI FseI FspAI HincII HindIII HpaI KpnI MluI NdeI NheI NotI NruI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI