################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AspEI AspI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BseRI BsiEI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI Ksp632I KspI MfeI MflI MluI MroI MslI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI PvuII RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I XbaI XcmI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 206 GGTACC G^GTACC AflII 1432 CTTAAG C^TTAAG Alw44I 607 GTGCAC G^TGCAC AlwI 269 GGATC GGATC(4/5) ApaLI 607 GTGCAC G^TGCAC AseI 688 ATTAAT AT^TAAT AsnI 688 ATTAAT AT^TAAT Asp700I 583 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 206 GGTACC G^GTACC AspHI 607 GTGCAC G[AT]GC[AT]^C AvaI 299 CTCGAG C^[CT]CG[GA]G BanI 206 GGTACC G^G[CT][GA]CC BcgI 921 GCATGTGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 409 CCGGG CC^[GC]GG BfaI 1087 CTAG C^TAG BfrI 1432 CTTAAG C^TTAAG BmyI 607 GTGCAC G[AGT]GC[ACT]^C BsaBI 9 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 607 GTGCAC G[AT]GC[AT]^C BsiWI 839 CGTACG C^GTACG BsmI 803 GCATTC GAATGC(1/-1) BsoBI 299 CTCGAG C^[CT]CG[GA]G Bsp1286I 607 GTGCAC G[AGT]GC[ACT]^C BssSI 215 CACGAG CACGAG(-5/-1) BstXI 1107 CCAGCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 1576 AGGACCC [GA]G^G[ACGT]CC[CT] DsaI 493 CCACGG C^C[GA][CT]GG EcoNI 1621 CCTTGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1576 AGGACCC [GA]G^G[ACGT]CC[CT] HapII 409 CCGG C^CGG HindIII 1509 AAGCTT A^AGCTT HpaI 748 GTTAAC GTT^AAC HpaII 409 CCGG C^CGG HphI 909 TCACC GGTGA(8/7) KpnI 206 GGTACC GGTAC^C MaeI 1087 CTAG C^TAG MaeII 946 ACGT A^CGT MamI 9 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 797 CAGCGG C[AC]G^C[GT]G MspI 409 CCGG C^CGG MwoI 1408 GCCTCTCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 409 CCGGG CC^[GC]GG NdeI 1673 CATATG CA^TATG PaeR7I 299 CTCGAG C^TCGAG PpuMI 1576 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1153 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 688 ATTAAT AT^TAAT PstI 479 CTGCAG CTGCA^G SalI 825 GTCGAC G^TCGAC SspI 996 AATATT AAT^ATT TfiI 548 GAATC G^A[AT]TC XhoI 299 CTCGAG C^TCGAG XmnI 583 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 825 GTCGAC GT^[AC][GT]AC 1222 GTCTAC BalI 319 TGGCCA TGG^CCA 941 TGGCCA BbsI 583 GAAGAC GAAGAC(2/6) 988 GAAGAC BpuAI 583 GAAGAC GAAGAC(2/6) 988 GAAGAC BsaI 1198 GAGACC GGTCTC(1/5) 296 GGTCTC BsmFI 1138 GGGAC GGGAC(10/14) 847 GTCCC BspHI 395 TCATGA T^CATGA 1181 TCATGA BsrDI 965 GCAATG GCAATG(2/0) 1695 GCAATG DpnII 270 GATC ^GATC 346 GATC EaeI 319 TGGCCA [CT]^GGCC[GA] 941 TGGCCA EcoRV 355 GATATC GAT^ATC 598 GATATC MaeIII 220 GTTAC ^GT[ACGT]AC 1299 GTTAC MboI 270 GATC ^GATC 346 GATC MluNI 319 TGGCCA TGG^CCA 941 TGGCCA MscI 319 TGGCCA TGG^CCA 941 TGGCCA NdeII 270 GATC ^GATC 346 GATC NspI 486 GCATGT [GA]CATG^[CT] 921 GCATGT PflMI 96 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1669 CCACCATATGG RcaI 395 TCATGA T^CATGA 1181 TCATGA Sau3AI 270 GATC ^GATC 346 GATC SfaNI 1608 GATGC GCATC(5/9) 14 GCATC SmlI 299 CTCGAG C^T[CT][GA]AG 1432 CTTAAG Van91I 96 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1669 CCACCATATGG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII BbvI BpmI BsaJI BsgI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HincII HindII HinfI ItaI MboII MnlI MseI MvaI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfcI StyI TaqI Tru9I TseI Tsp509I TspRI TthHB8I