(Circular) (Six-base) MAPSORT of: pFN21AB5853.seq Check: 4971 from: 1 to: 5671 pFN21AB5853 5671 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3481 278 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2801 2880 3014 1937 Size: 864 79 134 4594 Fragments arranged by size: 4594 864 134 79 Acc65I G'GTAC_C Cuts at: 2992 2992 Size: 5671 AclI AA'CG_TT Cuts at: 1563 3798 4171 1563 Size: 2235 373 3063 Fragments arranged by size: 3063 2235 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3734 5118 5386 1632 Size: 2102 1384 268 1917 Fragments arranged by size: 2102 1917 1384 268 AfeI AGC'GCT Cuts at: 2273 2273 Size: 5671 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5474 AflIII A'CryG_T Cuts at: 1203 4570 5547 1203 Size: 3367 977 1327 Fragments arranged by size: 3367 1327 977 AgeI A'CCGG_T Cuts at: 4502 4502 Size: 5671 AhdI GACnn_n'nnGTC Cuts at: 2179 4400 2179 Size: 2221 3450 AleI CACnn'nnGTG Cuts at: 1166 2263 2396 1166 Size: 1097 133 4441 Fragments arranged by size: 4441 1097 133 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5671 AlwNI CAG_nnn'CTG Cuts at: 3414 4986 3414 Size: 1572 4099 ApaLI G'TGCA_C Cuts at: 3728 4884 3728 Size: 1156 4515 ApoI r'AATT_y Cuts at: 1493 2980 3206 3358 5467 1493 Size: 1487 226 152 2109 1697 Fragments arranged by size: 2109 1697 1487 226 152 AseI AT'TA_AT Cuts at: 160 1032 4225 160 Size: 872 3193 1606 Fragments arranged by size: 3193 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 AvaI C'yCGr_G Cuts at: 1870 1943 2996 1870 Size: 73 1053 4545 Fragments arranged by size: 4545 1053 73 AvrII C'CTAG_G Cuts at: 2746 2746 Size: 5671 BamHI G'GATC_C Cuts at: 3001 3368 3001 Size: 367 5304 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2992 4447 618 Size: 325 217 627 1205 1455 1842 Fragments arranged by size: 1842 1455 1205 627 325 217 BanII G_rGCy'C Cuts at: 1391 2349 2990 1391 Size: 958 641 4072 Fragments arranged by size: 4072 958 641 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4700 BbvCI CC'TCA_GC Cuts at: 2204 2204 Size: 5671 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3862 3896 1903 Size: 34 1925 34 3678 Fragments arranged by size: 3678 1925 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3563 4773 3563 Size: 1210 4461 BclI T'GATC_A Cuts at: 1548 1548 Size: 5671 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4282 136 Size: 107 122 71 631 605 2610 1525 Fragments arranged by size: 2610 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5666 5666 Size: 5671 BlpI GC'TnA_GC Cuts at: 3088 3088 Size: 5671 Bme1580I G_kGCm'C Cuts at: 1790 3732 4888 1790 Size: 1942 1156 2573 Fragments arranged by size: 2573 1942 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5671 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2281 3402 4360 Size: 416 53 83 399 24 864 1121 958 Cuts at: 4360 442 Size: 1753 Fragments arranged by size: 1753 1121 958 864 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5671 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2531 4331 1239 Size: 1292 1800 2579 Fragments arranged by size: 2579 1800 1292 Bpu10I CC'TnA_GC Cuts at: 2204 2936 2204 Size: 732 4939 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3796 4661 4959 3796 Size: 865 298 4508 Fragments arranged by size: 4508 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2926 4334 882 Size: 617 1427 1408 2219 Fragments arranged by size: 2219 1427 1408 617 BsaAI yAC'GTr Cuts at: 493 1204 3462 493 Size: 711 2258 2702 Fragments arranged by size: 2702 2258 711 BsaBI GATnn'nnATC Cuts at: 5665 5665 Size: 5671 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3478 3860 4495 275 Size: 53 83 186 936 1945 382 635 1451 Fragments arranged by size: 1945 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4104 4502 4776 4923 5334 4104 Size: 398 274 147 411 4441 Fragments arranged by size: 4441 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5446 Fragments arranged by size: 5446 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5671 BseYI C'CCAG_C Cuts at: 2151 2361 2465 2476 4874 5370 2151 Size: 210 104 11 2398 496 2452 Fragments arranged by size: 2452 2398 496 210 104 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5098 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3121 3882 4910 1540 Size: 275 186 1120 761 1028 2301 Fragments arranged by size: 2301 1120 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2022 3732 3817 4888 2022 Size: 1710 85 1071 2805 Fragments arranged by size: 2805 1710 1071 85 BsmI GAATG_Cn' Cuts at: 2129 3182 3275 2129 Size: 1053 93 4525 Fragments arranged by size: 4525 1053 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2349 2990 3732 3817 4888 1391 Size: 399 232 327 641 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 641 399 327 232 85 BspEI T'CCGG_A Cuts at: 5334 5334 Size: 5671 BspHI T'CATG_A Cuts at: 3560 5290 3560 Size: 1730 3941 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3026 844 Size: 2182 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3558 1394 Size: 429 1735 3507 Fragments arranged by size: 3507 1735 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2678 4166 4340 65 Size: 1183 1430 1488 174 1396 Fragments arranged by size: 1488 1430 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4315 4502 1700 Size: 110 2505 187 2869 Fragments arranged by size: 2869 2505 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5671 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5671 BssSI C'ACGA_G Cuts at: 3731 4743 3731 Size: 1012 4659 BstAPI GCAn_nnn'nTGC Cuts at: 794 2785 794 Size: 1991 3680 BstBI TT'CG_AA Cuts at: 4475 4475 Size: 5671 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5671 BstXI CCAn_nnnn'nTGG Cuts at: 2239 2239 Size: 5671 BstYI r'GATC_y Cuts at: 1972 2910 3001 3368 3753 3770 5222 5308 5320 Size: 938 91 367 385 17 1452 86 12 Cuts at: 5320 5666 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 938 385 367 346 91 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2252 2252 Size: 5671 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4180 Fragments arranged by size: 4180 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2129 487 Size: 683 56 903 4029 Fragments arranged by size: 4029 903 683 56 BtsI GCAGTG_nn' Cuts at: 2124 2471 3187 3981 4001 2124 Size: 347 716 794 20 3794 Fragments arranged by size: 3794 794 716 347 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2575 2610 2838 2873 529 Size: 35 2011 35 228 35 3327 Fragments arranged by size: 3327 2011 228 35 35 35 DraI TTT'AAA Cuts at: 2975 2975 Size: 5671 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4678 809 Size: 507 3362 1802 Fragments arranged by size: 3362 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2494 2553 3118 Size: 54 1425 50 275 18 664 59 565 Cuts at: 3118 4007 5636 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 664 565 275 59 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3118 1537 Size: 275 1306 4090 Fragments arranged by size: 4090 1306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2825 3601 1353 Size: 1472 776 3423 Fragments arranged by size: 3423 1472 776 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4258 4632 4778 387 Size: 1064 138 2669 374 146 1280 Fragments arranged by size: 2669 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2531 3734 4331 5118 5386 1239 Size: 393 899 1203 597 787 268 1524 Fragments arranged by size: 1524 1203 899 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2207 2207 Size: 5671 EcoO109I rG'GnC_Cy Cuts at: 2155 2199 2313 2410 2155 Size: 44 114 97 5416 Fragments arranged by size: 5416 114 97 44 EcoRI G'AATT_C Cuts at: 2980 3358 2980 Size: 378 5293 FspI TGC'GCA Cuts at: 4177 4177 Size: 5671 HaeII r_GCGC'y Cuts at: 1678 2275 4818 1678 Size: 597 2543 2531 Fragments arranged by size: 2543 2531 597 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 2363 2395 2508 Size: 32 135 32 593 32 166 32 113 Cuts at: 2508 2540 4312 4344 4386 4418 1373 Size: 32 1772 32 42 32 2626 Fragments arranged by size: 2626 1772 593 166 135 113 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3015 3261 669 Size: 988 281 1077 246 3079 Fragments arranged by size: 3079 1077 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5671 HpaI GTT'AAC Cuts at: 3261 3261 Size: 5671 KpnI G_GTAC'C Cuts at: 2996 2996 Size: 5671 MfeI C'AATT_G Cuts at: 3270 3270 Size: 5671 MluI A'CGCG_T Cuts at: 5547 5547 Size: 5671 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2094 2494 4785 4969 1305 Size: 789 400 2291 184 2007 Fragments arranged by size: 2291 2007 789 400 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2496 2555 10 Size: 54 1425 343 664 59 3126 Fragments arranged by size: 3126 1425 664 343 59 54 MslI CAynn'nnrTG Cuts at: 518 1166 2263 2396 3629 3988 4147 518 Size: 648 1097 133 1233 359 159 2042 Fragments arranged by size: 2042 1233 1097 648 359 159 133 MspA1I CmG'CkG Cuts at: 1606 2551 3087 3764 4912 5157 1606 Size: 945 536 677 1148 245 2120 Fragments arranged by size: 2120 1148 945 677 536 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4180 Fragments arranged by size: 4180 939 552 NdeI CA'TA_TG Cuts at: 387 2528 387 Size: 2141 3530 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5671 NotI GC'GGCC_GC Cuts at: 3118 3118 Size: 5671 NspI r_CATG'y Cuts at: 1132 1229 3029 4574 1132 Size: 97 1800 1545 2229 Fragments arranged by size: 2229 1800 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5671 PciI A'CATG_T Cuts at: 4570 4570 Size: 5671 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5671 PfoI T'CCnGG_A Cuts at: 2022 2163 2022 Size: 141 5530 PmeI GTTT'AAAC Cuts at: 2975 2975 Size: 5671 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3740 3772 5253 5285 3740 Size: 32 1481 32 4126 Fragments arranged by size: 4126 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2155 2199 2313 2410 2155 Size: 44 114 97 5416 Fragments arranged by size: 5416 114 97 44 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5671 PsiI TTA'TAA Cuts at: 3241 3241 Size: 5671 PstI C_TGCA'G Cuts at: 830 3023 830 Size: 2193 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5671 PvuII CAG'CTG Cuts at: 2551 2551 Size: 5671 SalI G'TCGA_C Cuts at: 1936 3013 1936 Size: 1077 4594 SanDI GG'GwC_CC Cuts at: 2155 2199 2155 Size: 44 5627 SbfI CC_TGCA'GG Cuts at: 3023 3023 Size: 5671 ScaI AGT'ACT Cuts at: 3919 5631 3919 Size: 1712 3959 SexAI A'CCwGG_T Cuts at: 2412 2412 Size: 5671 SfcI C'TryA_G Cuts at: 826 1045 2725 3019 4154 4835 5026 826 Size: 219 1680 294 1135 681 191 1471 Fragments arranged by size: 1680 1471 1135 681 294 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5671 SmaI CCC'GGG Cuts at: 1872 2998 1872 Size: 1126 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3775 4676 4938 820 Size: 197 926 1832 901 262 1553 Fragments arranged by size: 1832 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3462 493 Size: 2969 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5671 SphI G_CATG'C Cuts at: 1132 3029 1132 Size: 1897 3774 SspI AAT'ATT Cuts at: 5 52 2107 3595 5 Size: 47 2055 1488 2081 Fragments arranged by size: 2081 2055 1488 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5671 StyI C'CwwG_G Cuts at: 513 1065 2004 2746 3110 513 Size: 552 939 742 364 3074 Fragments arranged by size: 3074 939 742 552 364 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2024 2353 2821 2908 3698 3715 3868 Size: 1255 444 329 468 87 790 17 153 Cuts at: 3868 4053 4498 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 790 468 445 444 329 185 153 87 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3917 5629 96 Size: 274 80 33 51 1443 1940 1712 138 Fragments arranged by size: 1940 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5631 5663 5631 Size: 32 5639 Tth111I GACn'n_nGTC Cuts at: 1316 2797 1316 Size: 1481 4190 XbaI T'CTAG_A Cuts at: 3007 3007 Size: 5671 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2641 2641 Size: 5671 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5671 XmaI C'CCGG_G Cuts at: 1870 2996 1870 Size: 1126 4545 XmnI GAAnn'nnTTC Cuts at: 3800 3800 Size: 5671 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3479 276 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI