################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaHI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoT22I FbaI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI MaeII MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 989 CGCG CG^CG AcsI 1056 GAATTT [GA]^AATT[CT] AfaI 1640 GTAC GT^AC AgeI 494 ACCGGT A^CCGGT ApaI 241 GGGCCC GGGCC^C ApoI 1056 GAATTT [GA]^AATT[CT] Asp700I 1360 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 455 GGATCC G^GATCC BcgI 557 GCACCATCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1249 CTAG C^TAG BglI 270 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 516 GCTGAGC GC^T[ACGT]AGC Bpu1102I 516 GCTGAGC GC^T[ACGT]AGC BsaI 1540 GAGACC GGTCTC(1/5) BsaWI 494 ACCGGT [AT]^CCGG[AT] BsgI 405 GTGCAG GTGCAG(16/14) BsiEI 991 CGGTCG CG[GA][CT]^CG BsmAI 1540 GAGAC GTCTC(1/5) BsmI 1397 GCATTC GAATGC(1/-1) BspHI 86 TCATGA T^CATGA BsrBI 1555 CCGCTC CCGCTC(-3/-3) BsrDI 1503 CATTGC GCAATG(2/0) BstEII 684 GGTCACC G^GT[ACGT]ACC BstPI 684 GGTCACC G^GT[ACGT]ACC BstUI 989 CGCG CG^CG BstYI 455 GGATCC [GA]^GATC[CT] CelII 516 GCTGAGC GC^T[ACGT]AGC EcoNI 1125 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 684 GGTCACC G^GT[ACGT]ACC EcoRV 813 GATATC GAT^ATC FseI 1233 GGCCGGCC GGCCGG^CC HaeII 729 GGCGCT [GA]GCGC^[CT] HgaI 365 GCGTC GACGC(5/10) KspI 988 CCGCGG CCGC^GG MaeI 1249 CTAG C^TAG MflI 455 GGATCC [GA]^GATC[CT] MseI 854 TTAA T^TAA MvnI 989 CGCG CG^CG NcoI 1229 CCATGG C^CATGG PinAI 494 ACCGGT A^CCGGT PleI 768 GACTC GAGTC(4/5) RcaI 86 TCATGA T^CATGA RsaI 1640 GTAC GT^AC SacI 883 GAGCTC GAGCT^C SacII 988 CCGCGG CCGC^GG SbfI 54 CCTGCAGG CCTGCA^GG SexAI 398 ACCTGGT A^CC[AT]GGT Sse8387I 54 CCTGCAGG CCTGCA^GG TfiI 832 GAATC G^A[AT]TC Tru9I 854 TTAA T^TAA Tsp509I 1057 AATT ^AATT XhoII 455 GGATCC [GA]^GATC[CT] XmnI 1360 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BanI 550 GGCACC G^G[CT][GA]CC 1263 GGTGCC BbsI 367 GTCTTC GAAGAC(2/6) 1090 GTCTTC BpuAI 367 GTCTTC GAAGAC(2/6) 1090 GTCTTC BstAPI 1269 GCAAGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1278 GCAGACCCTGC DrdI 343 GACGGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 358 GACCGCAGCGTC EarI 43 CTCTTC CTCTTC(1/4) 1138 CTCTTC HinfI 768 GACTC G^A[ACGT]TC 832 GAATC Ksp632I 43 CTCTTC CTCTTC(1/4) 1138 CTCTTC NspI 155 ACATGC [GA]CATG^[CT] 1256 ACATGC PvuII 309 CAGCTG CAG^CTG 775 CAGCTG TaqI 10 TCGA T^CGA 1094 TCGA TthHB8I 10 TCGA T^CGA 1094 TCGA XcmI 389 CCAAGTGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 689 CCATGACGGTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI AvaII BalI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PflMI PpuMI PstI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI Van91I