(Circular) (Six-base) MAPSORT of: pFN21AE4517.seq Check: 7787 from: 1 to: 6355 pFN21AE4517 6355 bp With 182 enzymes: SgfI * March 28, 2012 14:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2053 2053 Size: 6355 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4165 278 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3698 1937 Size: 1761 4594 Acc65I G'GTAC_C Cuts at: 3676 3676 Size: 6355 AclI AA'CG_TT Cuts at: 1563 4482 4855 1563 Size: 2919 373 3063 Fragments arranged by size: 3063 2919 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2701 2867 4418 5802 6070 1632 Size: 1069 166 1551 1384 268 1917 Fragments arranged by size: 1917 1551 1384 1069 268 166 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6158 AflIII A'CryG_T Cuts at: 1203 5254 6231 1203 Size: 4051 977 1327 Fragments arranged by size: 4051 1327 977 AgeI A'CCGG_T Cuts at: 2499 5186 2499 Size: 2687 3668 AhdI GACnn_n'nnGTC Cuts at: 5084 5084 Size: 6355 AleI CACnn'nnGTG Cuts at: 1166 2387 1166 Size: 1221 5134 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6355 AlwNI CAG_nnn'CTG Cuts at: 2059 3112 3289 3583 4098 5670 2059 Size: 1053 177 294 515 1572 2744 Fragments arranged by size: 2744 1572 1053 515 294 177 ApaI G_GGCC'C Cuts at: 2250 2250 Size: 6355 ApaLI G'TGCA_C Cuts at: 4412 5568 4412 Size: 1156 5199 ApoI r'AATT_y Cuts at: 1493 3061 3664 3890 4042 6151 1493 Size: 1568 603 226 152 2109 1697 Fragments arranged by size: 2109 1697 1568 603 226 152 AseI AT'TA_AT Cuts at: 160 1032 4909 160 Size: 872 3877 1606 Fragments arranged by size: 3877 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 AvaI C'yCGr_G Cuts at: 1870 1943 3680 1870 Size: 73 1737 4545 Fragments arranged by size: 4545 1737 73 BamHI G'GATC_C Cuts at: 2460 3685 4052 2460 Size: 1225 367 4763 Fragments arranged by size: 4763 1225 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2555 3268 3676 5131 618 Size: 325 217 627 768 713 408 1455 1842 Fragments arranged by size: 1842 1455 768 713 627 408 325 217 BanII G_rGCy'C Cuts at: 1391 2250 2474 2892 3674 1391 Size: 859 224 418 782 4072 Fragments arranged by size: 4072 859 782 418 224 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2365 3088 928 Size: 971 466 723 4195 Fragments arranged by size: 4195 971 723 466 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2551 2585 4546 4580 1903 Size: 34 614 34 1961 34 3678 Fragments arranged by size: 3678 1961 614 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4247 5457 4247 Size: 1210 5145 BclI T'GATC_A Cuts at: 1548 1548 Size: 6355 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2281 4966 136 Size: 107 122 71 631 605 609 2685 1525 Fragments arranged by size: 2685 1525 631 609 605 122 107 71 BglII A'GATC_T Cuts at: 6350 6350 Size: 6355 BlpI GC'TnA_GC Cuts at: 2522 3772 2522 Size: 1250 5105 Bme1580I G_kGCm'C Cuts at: 1790 2250 2558 2799 3024 4416 5572 1790 Size: 460 308 241 225 1392 1156 2573 Fragments arranged by size: 2573 1392 1156 460 308 241 225 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6355 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4086 5044 442 Size: 416 53 83 399 24 2669 958 1753 Fragments arranged by size: 2669 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6355 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2140 2995 2140 Size: 855 5500 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 2338 2762 2788 3055 3119 3605 5015 Size: 896 203 424 26 267 64 486 1410 Cuts at: 5015 1239 Size: 2579 Fragments arranged by size: 2579 1410 896 486 424 267 203 64 26 Bpu10I CC'TnA_GC Cuts at: 2435 2435 Size: 6355 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4480 5345 5643 4480 Size: 865 298 5192 Fragments arranged by size: 5192 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3539 5018 882 Size: 617 2040 1479 2219 Fragments arranged by size: 2219 2040 1479 617 BsaAI yAC'GTr Cuts at: 493 1204 4146 493 Size: 711 2942 2702 Fragments arranged by size: 2942 2702 711 BsaBI GATnn'nnATC Cuts at: 6349 6349 Size: 6355 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4162 4544 5179 275 Size: 53 83 186 936 2629 382 635 1451 Fragments arranged by size: 2629 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2499 4788 5186 5460 5607 6018 2499 Size: 2289 398 274 147 411 2836 Fragments arranged by size: 2836 2289 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2981 3011 3546 3576 1178 Size: 30 165 30 1578 30 535 30 3957 Fragments arranged by size: 3957 1578 535 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2480 2984 3052 3122 3474 3479 1186 Size: 1294 504 68 70 352 5 4062 Fragments arranged by size: 4062 1294 504 352 70 68 5 BseYI C'CCAG_C Cuts at: 2352 2737 3310 3424 5558 6054 2352 Size: 385 573 114 2134 496 2653 Fragments arranged by size: 2653 2134 573 496 385 114 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2431 1163 Size: 573 695 5087 Fragments arranged by size: 5087 695 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2999 3805 4566 5594 1540 Size: 275 186 998 806 761 1028 2301 Fragments arranged by size: 2301 1028 998 806 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2070 2892 3030 4416 4501 5572 2070 Size: 822 138 1386 85 1071 2853 Fragments arranged by size: 2853 1386 1071 822 138 85 BsmI GAATG_Cn' Cuts at: 3402 3866 3959 3402 Size: 464 93 5798 Fragments arranged by size: 5798 464 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2250 2474 2558 2799 2892 3024 Size: 399 280 180 224 84 241 93 132 Cuts at: 3024 3030 3674 4416 4501 5572 1391 Size: 6 644 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 644 399 280 241 224 180 132 93 85 84 6 BspEI T'CCGG_A Cuts at: 6018 6018 Size: 6355 BspHI T'CATG_A Cuts at: 2091 4244 5974 2091 Size: 2153 1730 2472 Fragments arranged by size: 2472 2153 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2053 3111 3350 3710 844 Size: 1209 1058 239 360 3489 Fragments arranged by size: 3489 1209 1058 360 239 BsrBI CCG'CTC Cuts at: 1394 1823 3562 4242 1394 Size: 429 1739 680 3507 Fragments arranged by size: 3507 1739 680 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3507 4850 5024 65 Size: 1183 2259 1343 174 1396 Fragments arranged by size: 2259 1396 1343 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2499 2627 3239 3420 3618 4999 5186 Size: 110 689 128 612 181 198 1381 187 Cuts at: 5186 1700 Size: 2869 Fragments arranged by size: 2869 1381 689 612 198 187 181 128 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6355 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6355 BssSI C'ACGA_G Cuts at: 4415 5427 4415 Size: 1012 5343 BstAPI GCAn_nnn'nTGC Cuts at: 794 3280 3289 794 Size: 2486 9 3860 Fragments arranged by size: 3860 2486 9 BstBI TT'CG_AA Cuts at: 5159 5159 Size: 6355 BstEII G'GTnAC_C Cuts at: 1184 2689 1184 Size: 1505 4850 BstXI CCAn_nnnn'nTGG Cuts at: 2389 2701 3055 3265 2389 Size: 312 354 210 5479 Fragments arranged by size: 5479 354 312 210 BstYI r'GATC_y Cuts at: 1972 2460 3685 4052 4437 4454 5906 5992 6004 Size: 488 1225 367 385 17 1452 86 12 Cuts at: 6004 6350 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1225 488 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2304 2547 2993 3153 3234 513 Size: 552 939 300 243 446 160 81 3634 Fragments arranged by size: 3634 939 552 446 300 243 160 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5616 Fragments arranged by size: 5616 683 56 BtsI GCAGTG_nn' Cuts at: 3177 3871 4665 4685 3177 Size: 694 794 20 4847 Fragments arranged by size: 4847 794 694 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6355 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6320 DraI TTT'AAA Cuts at: 3659 3659 Size: 6355 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2354 2369 5362 809 Size: 507 1038 15 2993 1802 Fragments arranged by size: 2993 1802 1038 507 15 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2307 2391 2544 Size: 54 1425 50 275 18 477 84 153 Cuts at: 2544 2610 2625 2901 2914 3202 3237 3802 4691 Size: 66 15 276 13 288 35 565 889 Cuts at: 4691 6320 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 565 477 288 276 275 153 84 66 54 50 43 35 18 15 13 EagI C'GGCC_G Cuts at: 1537 1812 3802 1537 Size: 275 1990 4090 Fragments arranged by size: 4090 1990 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 3149 4285 1353 Size: 701 1095 1136 3423 Fragments arranged by size: 3423 1136 1095 701 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2258 2830 4942 5316 5462 387 Size: 1064 138 669 572 2112 374 146 1280 Fragments arranged by size: 2112 1280 1064 669 572 374 146 138 EcoICRI GAG'CTC Cuts at: 2890 2890 Size: 6355 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2338 2701 2762 2788 2867 3055 Size: 393 503 203 363 61 26 79 188 Cuts at: 3055 3119 3605 4418 5015 5802 6070 1239 Size: 64 486 813 597 787 268 1524 Fragments arranged by size: 1524 813 787 597 503 486 393 363 268 203 188 79 64 61 26 EcoNI CCTnn'n_nnAGG Cuts at: 3134 3134 Size: 6355 EcoO109I rG'GnC_Cy Cuts at: 2206 2234 2246 2247 2401 2485 3139 3440 3580 Size: 28 12 1 154 84 654 301 140 Cuts at: 3580 2206 Size: 4981 Fragments arranged by size: 4981 654 301 154 140 84 28 12 1 EcoRI G'AATT_C Cuts at: 3664 4042 3664 Size: 378 5977 EcoRV GAT'ATC Cuts at: 2820 2820 Size: 6355 FseI GG_CCGG'CC Cuts at: 3243 3243 Size: 6355 FspI TGC'GCA Cuts at: 4861 4861 Size: 6355 HaeII r_GCGC'y Cuts at: 1678 2738 5502 1678 Size: 1060 2764 2531 Fragments arranged by size: 2764 2531 1060 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2159 2191 2981 3013 3546 Size: 32 135 32 587 32 790 32 533 Cuts at: 3546 3578 4996 5028 5070 5102 1373 Size: 32 1418 32 42 32 2626 Fragments arranged by size: 2626 1418 790 587 533 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3699 3945 669 Size: 988 281 1761 246 3079 Fragments arranged by size: 3079 1761 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6355 HpaI GTT'AAC Cuts at: 3945 3945 Size: 6355 KpnI G_GTAC'C Cuts at: 3680 3680 Size: 6355 MfeI C'AATT_G Cuts at: 3954 3954 Size: 6355 MluI A'CGCG_T Cuts at: 6231 6231 Size: 6355 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3398 5469 5653 1305 Size: 2093 2071 184 2007 Fragments arranged by size: 2093 2071 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2393 2903 2916 10 Size: 54 1425 343 561 510 13 3449 Fragments arranged by size: 3449 1425 561 510 343 54 13 MslI CAynn'nnrTG Cuts at: 518 1166 2192 2387 2450 2699 2945 3053 3266 Size: 648 1026 195 63 249 246 108 213 Cuts at: 3266 4313 4672 4831 518 Size: 1047 359 159 2042 Fragments arranged by size: 2042 1047 1026 648 359 249 246 213 195 159 108 63 MspA1I CmG'CkG Cuts at: 1606 2316 2782 2995 3033 3771 4448 5596 5841 Size: 710 466 213 38 738 677 1148 245 Cuts at: 5841 1606 Size: 2120 Fragments arranged by size: 2120 1148 738 710 677 466 245 213 38 NaeI GCC'GGC Cuts at: 2629 3241 3422 2629 Size: 612 181 5562 Fragments arranged by size: 5562 612 181 NcoI C'CATG_G Cuts at: 513 1065 2004 3234 513 Size: 552 939 1230 3634 Fragments arranged by size: 3634 1230 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6355 NgoMIV G'CCGG_C Cuts at: 2627 3239 3420 2627 Size: 612 181 5562 Fragments arranged by size: 5562 612 181 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6355 NotI GC'GGCC_GC Cuts at: 3802 3802 Size: 6355 NspI r_CATG'y Cuts at: 1132 1229 2164 3265 3713 5258 1132 Size: 97 935 1101 448 1545 2229 Fragments arranged by size: 2229 1545 1101 935 448 97 PasI CC'CwG_GG Cuts at: 1348 2419 3550 3610 1348 Size: 1071 1131 60 4093 Fragments arranged by size: 4093 1131 1071 60 PciI A'CATG_T Cuts at: 5254 5254 Size: 6355 PflMI CCAn_nnn'nTGG Cuts at: 1108 2316 3235 3583 1108 Size: 1208 919 348 3880 Fragments arranged by size: 3880 1208 919 348 PfoI T'CCnGG_A Cuts at: 2112 2456 3540 3576 2112 Size: 344 1084 36 4891 Fragments arranged by size: 4891 1084 344 36 PmeI GTTT'AAAC Cuts at: 3659 3659 Size: 6355 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4424 4456 5937 5969 4424 Size: 32 1481 32 4810 Fragments arranged by size: 4810 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2401 3440 3580 2401 Size: 1039 140 5176 Fragments arranged by size: 5176 1039 140 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6355 PsiI TTA'TAA Cuts at: 3925 3925 Size: 6355 PspOMI G'GGCC_C Cuts at: 2246 2246 Size: 6355 PstI C_TGCA'G Cuts at: 830 2064 2541 3108 3707 830 Size: 1234 477 567 599 3478 Fragments arranged by size: 3478 1234 599 567 477 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6355 PvuII CAG'CTG Cuts at: 2316 2782 2316 Size: 466 5889 SacI G_AGCT'C Cuts at: 2892 2892 Size: 6355 SacII CC_GC'GG Cuts at: 2996 2996 Size: 6355 SalI G'TCGA_C Cuts at: 1936 3697 1936 Size: 1761 4594 SanDI GG'GwC_CC Cuts at: 3440 3440 Size: 6355 SbfI CC_TGCA'GG Cuts at: 2064 3707 2064 Size: 1643 4712 ScaI AGT'ACT Cuts at: 4603 6315 4603 Size: 1712 4643 SexAI A'CCwGG_T Cuts at: 2403 2403 Size: 6355 SfcI C'TryA_G Cuts at: 826 1045 2060 2537 3104 3703 4838 5519 5710 Size: 219 1015 477 567 599 1135 681 191 Cuts at: 5710 826 Size: 1471 Fragments arranged by size: 1471 1135 1015 681 599 567 477 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6355 SmaI CCC'GGG Cuts at: 1872 3682 1872 Size: 1810 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4459 5360 5622 820 Size: 197 926 2516 901 262 1553 Fragments arranged by size: 2516 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4146 493 Size: 3653 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6355 SphI G_CATG'C Cuts at: 1132 3713 1132 Size: 2581 3774 SspI AAT'ATT Cuts at: 5 52 4279 5 Size: 47 4227 2081 Fragments arranged by size: 4227 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6355 StyI C'CwwG_G Cuts at: 513 1065 2004 2202 3199 3234 3794 513 Size: 552 939 198 997 35 560 3074 Fragments arranged by size: 3074 997 939 560 552 198 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4382 4399 4552 4737 5182 325 Size: 1255 2802 17 153 185 445 1498 Fragments arranged by size: 2802 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4601 6313 96 Size: 274 80 33 51 1443 2624 1712 138 Fragments arranged by size: 2624 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2393 2425 6315 6347 2393 Size: 32 3890 32 2401 Fragments arranged by size: 3890 2401 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6355 XbaI T'CTAG_A Cuts at: 3691 3691 Size: 6355 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2401 2701 2401 Size: 300 6055 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6355 XmaI C'CCGG_G Cuts at: 1870 3680 1870 Size: 1810 4545 XmnI GAAnn'nnTTC Cuts at: 3369 4484 3369 Size: 1115 5240 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4163 276 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SfiI SfoI SrfI SwaI