(Linear) (Six-base) MAPSORT of: KIEE4517.seq Check: 5876 from: 1 to: 1650 KIEE4517.orf With 182 enzymes: SgfI * March 22, 2012 18:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 48 1650 Size: 48 1602 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 696 862 1650 Size: 696 166 788 Fragments arranged by size: 788 696 166 AgeI A'CCGG_T Cuts at: 0 494 1650 Size: 494 1156 AleI CACnn'nnGTG Cuts at: 0 382 1650 Size: 382 1268 AlwNI CAG_nnn'CTG Cuts at: 0 54 1107 1284 1578 1650 Size: 54 1053 177 294 72 Fragments arranged by size: 1053 294 177 72 54 ApaI G_GGCC'C Cuts at: 0 245 1650 Size: 245 1405 ApoI r'AATT_y Cuts at: 0 1056 1650 Size: 1056 594 BamHI G'GATC_C Cuts at: 0 455 1650 Size: 455 1195 BanI G'GyrC_C Cuts at: 0 550 1263 1650 Size: 550 713 387 Fragments arranged by size: 713 550 387 BanII G_rGCy'C Cuts at: 0 245 469 887 1650 Size: 245 224 418 763 Fragments arranged by size: 763 418 245 224 BbsI GAAGACnn'nnnn_ Cuts at: 0 360 1083 1650 Size: 360 723 567 Fragments arranged by size: 723 567 360 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 546 580 1650 Size: 546 34 1070 Fragments arranged by size: 1070 546 34 BglI GCCn_nnn'nGGC Cuts at: 0 276 1650 Size: 276 1374 BlpI GC'TnA_GC Cuts at: 0 517 1650 Size: 517 1133 Bme1580I G_kGCm'C Cuts at: 0 245 553 794 1019 1650 Size: 245 308 241 225 631 Fragments arranged by size: 631 308 245 241 225 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 135 990 1650 Size: 135 855 660 Fragments arranged by size: 855 660 135 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 333 757 783 1050 1114 1600 1650 Size: 130 203 424 26 267 64 486 50 Fragments arranged by size: 486 424 267 203 130 64 50 26 Bpu10I CC'TnA_GC Cuts at: 0 430 1650 Size: 430 1220 BsaI GGTCTCn'nnnn_ Cuts at: 0 1534 1650 Size: 1534 116 BsaWI w'CCGG_w Cuts at: 0 494 1650 Size: 494 1156 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 976 1006 1541 1571 1650 Size: 976 30 535 30 79 Fragments arranged by size: 976 535 79 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 475 979 1047 1117 1469 1474 1650 Size: 475 504 68 70 352 5 176 Fragments arranged by size: 504 475 352 176 70 68 5 BseYI C'CCAG_C Cuts at: 0 347 732 1305 1419 1650 Size: 347 385 573 114 231 Fragments arranged by size: 573 385 347 231 114 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 426 1650 Size: 426 1224 BsiEI CG_ry'CG Cuts at: 0 994 1650 Size: 994 656 BsiHKAI G_wGCw'C Cuts at: 0 65 887 1025 1650 Size: 65 822 138 625 Fragments arranged by size: 822 625 138 65 BsmI GAATG_Cn' Cuts at: 0 1397 1650 Size: 1397 253 Bsp1286I G_dGCh'C Cuts at: 0 65 245 469 553 794 887 1019 1025 Size: 65 180 224 84 241 93 132 6 Cuts at: 1025 1650 Size: 625 Fragments arranged by size: 625 241 224 180 132 93 84 65 6 BspHI T'CATG_A Cuts at: 0 86 1650 Size: 86 1564 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 48 1106 1345 1650 Size: 48 1058 239 305 Fragments arranged by size: 1058 305 239 48 BsrBI CCG'CTC Cuts at: 0 1557 1650 Size: 1557 93 BsrDI GCAATG_nn' Cuts at: 0 1502 1650 Size: 1502 148 BsrFI r'CCGG_y Cuts at: 0 494 622 1234 1415 1613 1650 Size: 494 128 612 181 198 37 Fragments arranged by size: 612 494 198 181 128 37 BstAPI GCAn_nnn'nTGC Cuts at: 0 1275 1284 1650 Size: 1275 9 366 Fragments arranged by size: 1275 366 9 BstEII G'GTnAC_C Cuts at: 0 684 1650 Size: 684 966 BstXI CCAn_nnnn'nTGG Cuts at: 0 384 696 1050 1260 1650 Size: 384 312 354 210 390 Fragments arranged by size: 390 384 354 312 210 BstYI r'GATC_y Cuts at: 0 455 1650 Size: 455 1195 BtgI C'CryG_G Cuts at: 0 299 542 988 1148 1229 1650 Size: 299 243 446 160 81 421 Fragments arranged by size: 446 421 299 243 160 81 BtsI GCAGTG_nn' Cuts at: 0 1172 1650 Size: 1172 478 DrdI GACnn_nn'nnGTC Cuts at: 0 349 364 1650 Size: 349 15 1286 Fragments arranged by size: 1286 349 15 EaeI y'GGCC_r Cuts at: 0 302 386 539 605 620 896 909 1197 Size: 302 84 153 66 15 276 13 288 Cuts at: 1197 1232 1650 Size: 35 418 Fragments arranged by size: 418 302 288 276 153 84 66 35 15 13 EarI CTCTTCn'nnn_ Cuts at: 0 49 1144 1650 Size: 49 1095 506 Fragments arranged by size: 1095 506 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 253 825 1650 Size: 253 572 825 Fragments arranged by size: 825 572 253 EcoICRI GAG'CTC Cuts at: 0 885 1650 Size: 885 765 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 333 696 757 783 862 1050 1114 Size: 130 203 363 61 26 79 188 64 Cuts at: 1114 1600 1650 Size: 486 50 Fragments arranged by size: 486 363 203 188 130 79 64 61 50 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 1129 1650 Size: 1129 521 EcoO109I rG'GnC_Cy Cuts at: 0 201 229 241 242 396 480 1134 1435 Size: 201 28 12 1 154 84 654 301 Cuts at: 1435 1575 1650 Size: 140 75 Fragments arranged by size: 654 301 201 154 140 84 75 28 12 1 EcoRV GAT'ATC Cuts at: 0 815 1650 Size: 815 835 FseI GG_CCGG'CC Cuts at: 0 1238 1650 Size: 1238 412 HaeII r_GCGC'y Cuts at: 0 733 1650 Size: 733 917 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 154 186 976 1008 1541 1573 1650 Size: 154 32 790 32 533 32 77 Fragments arranged by size: 790 533 154 77 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1393 1650 Size: 1393 257 MscI TGG'CCA Cuts at: 0 388 898 911 1650 Size: 388 510 13 739 Fragments arranged by size: 739 510 388 13 MslI CAynn'nnrTG Cuts at: 0 187 382 445 694 940 1048 1261 1650 Size: 187 195 63 249 246 108 213 389 Fragments arranged by size: 389 249 246 213 195 187 108 63 MspA1I CmG'CkG Cuts at: 0 311 777 990 1028 1650 Size: 311 466 213 38 622 Fragments arranged by size: 622 466 311 213 38 NaeI GCC'GGC Cuts at: 0 624 1236 1417 1650 Size: 624 612 181 233 Fragments arranged by size: 624 612 233 181 NcoI C'CATG_G Cuts at: 0 1229 1650 Size: 1229 421 NgoMIV G'CCGG_C Cuts at: 0 622 1234 1415 1650 Size: 622 612 181 235 Fragments arranged by size: 622 612 235 181 NspI r_CATG'y Cuts at: 0 159 1260 1650 Size: 159 1101 390 Fragments arranged by size: 1101 390 159 PasI CC'CwG_GG Cuts at: 0 414 1545 1605 1650 Size: 414 1131 60 45 Fragments arranged by size: 1131 414 60 45 PflMI CCAn_nnn'nTGG Cuts at: 0 311 1230 1578 1650 Size: 311 919 348 72 Fragments arranged by size: 919 348 311 72 PfoI T'CCnGG_A Cuts at: 0 107 451 1535 1571 1650 Size: 107 344 1084 36 79 Fragments arranged by size: 1084 344 107 79 36 PpuMI rG'GwC_Cy Cuts at: 0 396 1435 1575 1650 Size: 396 1039 140 75 Fragments arranged by size: 1039 396 140 75 PspOMI G'GGCC_C Cuts at: 0 241 1650 Size: 241 1409 PstI C_TGCA'G Cuts at: 0 59 536 1103 1650 Size: 59 477 567 547 Fragments arranged by size: 567 547 477 59 PvuII CAG'CTG Cuts at: 0 311 777 1650 Size: 311 466 873 Fragments arranged by size: 873 466 311 SacI G_AGCT'C Cuts at: 0 887 1650 Size: 887 763 SacII CC_GC'GG Cuts at: 0 991 1650 Size: 991 659 SanDI GG'GwC_CC Cuts at: 0 1435 1650 Size: 1435 215 SbfI CC_TGCA'GG Cuts at: 0 59 1650 Size: 59 1591 SexAI A'CCwGG_T Cuts at: 0 398 1650 Size: 398 1252 SfcI C'TryA_G Cuts at: 0 55 532 1099 1650 Size: 55 477 567 551 Fragments arranged by size: 567 551 477 55 StyI C'CwwG_G Cuts at: 0 197 1194 1229 1650 Size: 197 997 35 421 Fragments arranged by size: 997 421 197 35 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 388 420 1650 Size: 388 32 1230 Fragments arranged by size: 1230 388 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 396 696 1650 Size: 396 300 954 Fragments arranged by size: 954 396 300 XmnI GAAnn'nnTTC Cuts at: 0 1364 1650 Size: 1364 286 Enzymes that do cut: AarI AcuI AgeI AleI AlwNI ApaI ApoI BamHI BanI BanII BbsI BcgI BglI BlpI Bme1580I BplI BpmI Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI BtgI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FseI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI SbfI SexAI SfcI StyI TstI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BpuEI BsaAI BsaBI BsaHI BsiWI BsmBI BspEI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EcoRI FalI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI XmaI ZraI