################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## Acc65I AccI AccII AccIII AciI AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AvrII BanI BanII BbeI BbrPI BciVI BclI BfaI BfrI BlnI BlpI BpmI Bpu1102I BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI HaeII HgaI HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MseI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 298 GACGTC GACGT^C AcyI 298 GACGTC G[GA]^CG[CT]C AlwNI 597 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 306 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 624 GAGCAC G[AT]GC[AT]^C AviII 339 TGCGCA TGC^GCA BamHI 119 GGATCC G^GATCC BbsI 306 GAAGAC GAAGAC(2/6) BbvCI 177 GCTGAGG CCTCAGC(-5/-2) BcgI 244 GCACTCTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 91 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 205 AGATCT A^GATCT BmyI 624 GAGCAC G[AGT]GC[ACT]^C BpuAI 306 GAAGAC GAAGAC(2/6) BsaAI 561 CACGTA [CT]AC^GT[GA] BsaHI 298 GACGTC G[GA]^CG[CT]C BsgI 475 GTGCAG GTGCAG(16/14) BsiHKAI 624 GAGCAC G[AT]GC[AT]^C BsmAI 163 GAGAC GTCTC(1/5) Bsp1286I 624 GAGCAC G[AGT]GC[ACT]^C BsrI 116 ACTGG ACTGG(1/-1) CfoI 340 GCGC GCG^C DraIII 76 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 247 CTCTTC CTCTTC(1/4) FspI 339 TGCGCA TGC^GCA HhaI 340 GCGC GCG^C Hin1I 298 GACGTC G[GA]^CG[CT]C HinP1I 340 GCGC G^CGC HincII 34 GTCAAC GT[CT]^[GA]AC HindII 34 GTCAAC GT[CT]^[GA]AC HindIII 280 AAGCTT A^AGCTT Ksp632I 247 CTCTTC CTCTTC(1/4) MspA1I 263 CAGCTG C[AC]G^C[GT]G PvuII 263 CAGCTG CAG^CTG SexAI 5 ACCAGGT A^CC[AT]GGT SfiI 90 GGCCCGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TaqI 253 TCGA T^CGA Tsp45I 395 GTCAC ^GT[GC]AC TthHB8I 253 TCGA T^CGA XcmI 86 CCATGGCCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 306 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AlwI 120 GATCC GGATC(4/5) 119 GGATC AvaII 129 GGACC G^G[AT]CC 573 GGACC BalI 83 TGGCCA TGG^CCA 461 TGGCCA BbvI 64 GCAGC GCAGC(8/12) 262 GCAGC BcnI 92 CCCGG CC^[GC]GG 123 CCGGG BstYI 119 GGATCC [GA]^GATC[CT] 205 AGATCT EaeI 83 TGGCCA [CT]^GGCC[GA] 461 TGGCCA EcoNI 327 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 459 CCTGGCCAAGG Fnu4HI 64 GCAGC GC^[ACGT]GC 262 GCAGC HapII 93 CCGG C^CGG 123 CCGG HinfI 421 GAATC G^A[ACGT]TC 543 GATTC HpaII 93 CCGG C^CGG 123 CCGG HphI 75 TCACC GGTGA(8/7) 456 TCACC ItaI 64 GCAGC GC^[ACGT]GC 262 GCAGC MaeII 299 ACGT A^CGT 562 ACGT MaeIII 395 GTCAC ^GT[ACGT]AC 410 GTTAC MflI 119 GGATCC [GA]^GATC[CT] 205 AGATCT MluNI 83 TGGCCA TGG^CCA 461 TGGCCA MscI 83 TGGCCA TGG^CCA 461 TGGCCA MspI 93 CCGG C^CGG 123 CCGG NciI 92 CCCGG CC^[GC]GG 123 CCGGG PflMI 229 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 323 CCAACCTCTGG PstI 103 CTGCAG CTGCA^G 260 CTGCAG SfcI 103 CTGCAG C^T[GA][CT]AG 260 CTGCAG TfiI 421 GAATC G^A[AT]TC 543 GATTC TseI 64 GCAGC G^C[AT]GC 262 GCAGC Van91I 229 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 323 CCAACCTCTGG XhoII 119 GGATCC [GA]^GATC[CT] 205 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AfaI AluI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EcoRII EcoT14I FokI HaeIII MboI MboII MnlI MvaI MwoI NcoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TspRI