(Circular) (Six-base) MAPSORT of: pFN21AE2744.seq Check: 6887 from: 1 to: 5350 pFN21AE2744 5350 bp With 182 enzymes: SgfI * June 19, 2019 18:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2307 3160 278 Size: 53 83 186 936 771 853 2468 Fragments arranged by size: 2468 936 853 771 186 83 53 AccI GT'mk_AC Cuts at: 1937 2693 1937 Size: 756 4594 Acc65I G'GTAC_C Cuts at: 2671 2671 Size: 5350 AclI AA'CG_TT Cuts at: 1563 3477 3850 1563 Size: 1914 373 3063 Fragments arranged by size: 3063 1914 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2303 3413 4797 5065 1632 Size: 671 1110 1384 268 1917 Fragments arranged by size: 1917 1384 1110 671 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5153 AflIII A'CryG_T Cuts at: 1203 4249 5226 1203 Size: 3046 977 1327 Fragments arranged by size: 3046 1327 977 AgeI A'CCGG_T Cuts at: 4181 4181 Size: 5350 AhdI GACnn_n'nnGTC Cuts at: 4079 4079 Size: 5350 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5350 AlwNI CAG_nnn'CTG Cuts at: 2607 3093 4665 2607 Size: 486 1572 3292 Fragments arranged by size: 3292 1572 486 ApaLI G'TGCA_C Cuts at: 3407 4563 3407 Size: 1156 4194 ApoI r'AATT_y Cuts at: 1493 2659 2885 3037 5146 1493 Size: 1166 226 152 2109 1697 Fragments arranged by size: 2109 1697 1166 226 152 AseI AT'TA_AT Cuts at: 160 1032 3904 160 Size: 872 2872 1606 Fragments arranged by size: 2872 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5350 AvaI C'yCGr_G Cuts at: 1870 1943 2675 1870 Size: 73 732 4545 Fragments arranged by size: 4545 732 73 BamHI G'GATC_C Cuts at: 2124 2680 3047 2124 Size: 556 367 4427 Fragments arranged by size: 4427 556 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2671 4126 618 Size: 325 217 627 884 1455 1842 Fragments arranged by size: 1842 1455 884 627 325 217 BanII G_rGCy'C Cuts at: 1391 2669 1391 Size: 1278 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2318 928 Size: 971 419 3960 Fragments arranged by size: 3960 971 419 BbvCI CC'TCA_GC Cuts at: 2183 2183 Size: 5350 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2238 2272 3541 3575 1903 Size: 34 301 34 1269 34 3678 Fragments arranged by size: 3678 1269 301 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3242 4452 3242 Size: 1210 4140 BclI T'GATC_A Cuts at: 1548 1548 Size: 5350 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2102 3961 136 Size: 107 122 71 631 605 430 1859 1525 Fragments arranged by size: 1859 1525 631 605 430 122 107 71 BglII A'GATC_T Cuts at: 2210 5345 2210 Size: 3135 2215 BlpI GC'TnA_GC Cuts at: 2767 2767 Size: 5350 Bme1580I G_kGCm'C Cuts at: 1790 3411 4567 1790 Size: 1621 1156 2573 Fragments arranged by size: 2573 1621 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5350 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3081 4039 442 Size: 416 53 83 399 24 1664 958 1753 Fragments arranged by size: 1753 1664 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5350 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4010 1239 Size: 2771 2579 Bpu10I CC'TnA_GC Cuts at: 2183 2277 2183 Size: 94 5256 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3475 4340 4638 3475 Size: 865 298 4187 Fragments arranged by size: 4187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4013 882 Size: 617 2514 2219 Fragments arranged by size: 2514 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2568 3141 493 Size: 711 1364 573 2702 Fragments arranged by size: 2702 1364 711 573 BsaBI GATnn'nnATC Cuts at: 5344 5344 Size: 5350 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2304 3157 3539 4174 Size: 53 83 186 936 771 853 382 635 Cuts at: 4174 275 Size: 1451 Fragments arranged by size: 1451 936 853 771 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3783 4181 4455 4602 5013 3783 Size: 398 274 147 411 4120 Fragments arranged by size: 4120 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5125 Fragments arranged by size: 5125 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5350 BseYI C'CCAG_C Cuts at: 4553 5049 4553 Size: 496 4854 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2501 1163 Size: 573 765 4012 Fragments arranged by size: 4012 765 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2800 3561 4589 1540 Size: 275 186 799 761 1028 2301 Fragments arranged by size: 2301 1028 799 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2633 3411 3496 4567 2633 Size: 778 85 1071 3416 Fragments arranged by size: 3416 1071 778 85 BsmI GAATG_Cn' Cuts at: 2861 2954 2861 Size: 93 5257 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2633 2669 3411 3496 4567 1391 Size: 399 843 36 742 85 1071 2174 Fragments arranged by size: 2174 1071 843 742 399 85 36 BspEI T'CCGG_A Cuts at: 5013 5013 Size: 5350 BspHI T'CATG_A Cuts at: 3239 4969 3239 Size: 1730 3620 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2705 844 Size: 1861 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3237 1394 Size: 429 1414 3507 Fragments arranged by size: 3507 1414 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3845 4019 65 Size: 1183 2597 174 1396 Fragments arranged by size: 2597 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3994 4181 1700 Size: 110 2184 187 2869 Fragments arranged by size: 2869 2184 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5350 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5350 BssSI C'ACGA_G Cuts at: 3410 4422 3410 Size: 1012 4338 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5350 BstBI TT'CG_AA Cuts at: 4154 4154 Size: 5350 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5350 BstYI r'GATC_y Cuts at: 1972 2124 2210 2680 3047 3432 3449 4901 4987 Size: 152 86 470 367 385 17 1452 86 Cuts at: 4987 4999 5345 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 470 385 367 346 152 86 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2091 2239 2581 513 Size: 552 1026 148 342 3282 Fragments arranged by size: 3282 1026 552 342 148 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2404 487 Size: 683 56 1178 3433 Fragments arranged by size: 3433 1178 683 56 BtsI GCAGTG_nn' Cuts at: 2866 3660 3680 2866 Size: 794 20 4536 Fragments arranged by size: 4536 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5350 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5315 DraI TTT'AAA Cuts at: 2654 2654 Size: 5350 DraIII CAC_nnn'GTG Cuts at: 2086 2086 Size: 5350 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4357 809 Size: 507 3041 1802 Fragments arranged by size: 3041 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2466 2797 Size: 54 1425 50 275 18 258 378 331 Cuts at: 2797 3686 5315 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 378 331 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2797 1537 Size: 275 985 4090 Fragments arranged by size: 4090 985 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2258 3280 1353 Size: 905 1022 3423 Fragments arranged by size: 3423 1022 905 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3937 4311 4457 387 Size: 1064 138 2348 374 146 1280 Fragments arranged by size: 2348 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2303 3413 4010 4797 5065 1239 Size: 393 671 1110 597 787 268 1524 Fragments arranged by size: 1524 1110 787 671 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2336 2468 2336 Size: 132 5218 EcoRI G'AATT_C Cuts at: 2659 3037 2659 Size: 378 4972 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2269 2392 2269 Size: 123 5227 FspI TGC'GCA Cuts at: 2346 3856 2346 Size: 1510 3840 FspAI rTGC'GCAy Cuts at: 2346 2346 Size: 5350 HaeII r_GCGC'y Cuts at: 1678 4497 1678 Size: 2819 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2176 2208 3991 4023 4065 Size: 32 135 32 604 32 1783 32 42 Cuts at: 4065 4097 1373 Size: 32 2626 Fragments arranged by size: 2626 1783 604 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2041 2694 2940 669 Size: 988 281 103 653 246 3079 Fragments arranged by size: 3079 988 653 281 246 103 HindIII A'AGCT_T Cuts at: 748 2285 748 Size: 1537 3813 HpaI GTT'AAC Cuts at: 2940 2940 Size: 5350 KpnI G_GTAC'C Cuts at: 2675 2675 Size: 5350 MfeI C'AATT_G Cuts at: 2949 2949 Size: 5350 MluI A'CGCG_T Cuts at: 5226 5226 Size: 5350 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2142 4464 4648 1305 Size: 837 2322 184 2007 Fragments arranged by size: 2322 2007 837 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2090 2468 10 Size: 54 1425 343 258 378 2892 Fragments arranged by size: 2892 1425 378 343 258 54 MslI CAynn'nnrTG Cuts at: 518 1166 3308 3667 3826 518 Size: 648 2142 359 159 2042 Fragments arranged by size: 2142 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2270 2766 3443 4591 4836 1606 Size: 664 496 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 664 496 245 NcoI C'CATG_G Cuts at: 513 1065 2091 2239 2581 513 Size: 552 1026 148 342 3282 Fragments arranged by size: 3282 1026 552 342 148 NdeI CA'TA_TG Cuts at: 387 387 Size: 5350 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5350 NotI GC'GGCC_GC Cuts at: 2797 2797 Size: 5350 NspI r_CATG'y Cuts at: 1132 1229 2708 4253 1132 Size: 97 1479 1545 2229 Fragments arranged by size: 2229 1545 1479 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5350 PciI A'CATG_T Cuts at: 4249 4249 Size: 5350 PflMI CCAn_nnn'nTGG Cuts at: 1108 2240 2334 1108 Size: 1132 94 4124 Fragments arranged by size: 4124 1132 94 PfoI T'CCnGG_A Cuts at: 2352 2352 Size: 5350 PmeI GTTT'AAAC Cuts at: 2654 2654 Size: 5350 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3419 3451 4932 4964 3419 Size: 32 1481 32 3805 Fragments arranged by size: 3805 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5350 PsiI TTA'TAA Cuts at: 2920 2920 Size: 5350 PstI C_TGCA'G Cuts at: 830 2112 2269 2702 830 Size: 1282 157 433 3478 Fragments arranged by size: 3478 1282 433 157 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5350 PvuII CAG'CTG Cuts at: 2270 2270 Size: 5350 SalI G'TCGA_C Cuts at: 1936 2692 1936 Size: 756 4594 SbfI CC_TGCA'GG Cuts at: 2702 2702 Size: 5350 ScaI AGT'ACT Cuts at: 3598 5310 3598 Size: 1712 3638 SexAI A'CCwGG_T Cuts at: 2010 2010 Size: 5350 SfcI C'TryA_G Cuts at: 826 1045 2108 2265 2698 3833 4514 4705 826 Size: 219 1063 157 433 1135 681 191 1471 Fragments arranged by size: 1471 1135 1063 681 433 219 191 157 SfiI GGCCn_nnn'nGGCC Cuts at: 2102 2102 Size: 5350 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5350 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5350 SmaI CCC'GGG Cuts at: 1872 2677 1872 Size: 805 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3454 4355 4617 820 Size: 197 926 1511 901 262 1553 Fragments arranged by size: 1553 1511 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3141 493 Size: 2648 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5350 SphI G_CATG'C Cuts at: 1132 2708 1132 Size: 1576 3774 SspI AAT'ATT Cuts at: 5 52 3274 5 Size: 47 3222 2081 Fragments arranged by size: 3222 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5350 StyI C'CwwG_G Cuts at: 513 1065 2091 2138 2239 2469 2517 2524 2581 Size: 552 1026 47 101 230 48 7 57 Cuts at: 2581 2595 2789 513 Size: 14 194 3074 Fragments arranged by size: 3074 1026 552 230 194 101 57 48 47 14 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2639 3377 3394 3547 3732 4177 325 Size: 1255 1059 738 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1059 738 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3596 5308 96 Size: 274 80 33 51 1443 1619 1712 138 Fragments arranged by size: 1712 1619 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5310 5342 5310 Size: 32 5318 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5350 XbaI T'CTAG_A Cuts at: 2686 2686 Size: 5350 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2098 2098 Size: 5350 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5350 XmaI C'CCGG_G Cuts at: 1870 2675 1870 Size: 805 4545 XmnI GAAnn'nnTTC Cuts at: 2315 3479 2315 Size: 1164 4186 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2305 3158 276 Size: 53 83 186 936 771 853 2468 Fragments arranged by size: 2468 936 853 771 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfoI SrfI SwaI