################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AviII AvrII BamHI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MflI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NspI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcsI 313 AAATTC [GA]^AATT[CT] AhdI 463 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 230 AGCT AG^CT ApaI 322 GGGCCC GGGCC^C ApoI 313 AAATTC [GA]^AATT[CT] AspEI 463 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 244 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 325 CCCGAG C^[CT]CG[GA]G BalI 272 TGGCCA TGG^CCA BanI 28 GGCGCC G^G[CT][GA]CC BbeI 28 GGCGCC GGCGC^C BbrPI 496 CACGTG CAC^GTG BcgI 284 GCAAGGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 572 CCCGG CC^[GC]GG BglI 324 GCCCGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 46 CTGGAG CTGGAG(16/14) BsaAI 496 CACGTG [CT]AC^GT[GA] BseRI 523 GAGGAG GAGGAG(10/8) BsmFI 143 GGGAC GGGAC(10/14) BsoBI 325 CCCGAG C^[CT]CG[GA]G BspMI 92 ACCTGC ACCTGC(4/8) BsrFI 150 GCCGGC [GA]^CCGG[CT] Cfr10I 150 GCCGGC [GA]^CCGG[CT] Eam1105I 463 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 353 GAAGAG CTCTTC(1/4) EcoNI 387 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 359 ATGCAT ATGCA^T HgaI 248 GCGTC GACGC(5/10) HphI 218 TCACC GGTGA(8/7) KasI 28 GGCGCC G^GCGCC Ksp632I 353 GAAGAG CTCTTC(1/4) MluNI 272 TGGCCA TGG^CCA MscI 272 TGGCCA TGG^CCA MslI 264 CATACTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 159 CAGCGG C[AC]G^C[GT]G NaeI 150 GCCGGC GCC^GGC NarI 28 GGCGCC GG^CGCC NciI 572 CCCGG CC^[GC]GG NgoMIV 150 GCCGGC G^CCGGC NsiI 359 ATGCAT ATGCA^T PflFI 244 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 496 CACGTG CAC^GTG PmlI 496 CACGTG CAC^GTG PpuMI 254 AGGACCC [GA]G^G[AT]CC[CT] SfiI 323 GGCCCGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 28 GGCGCC GGC^GCC SmlI 202 CTCAAG C^T[CT][GA]AG Tsp509I 314 AATT ^AATT Tth111I 244 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 377 CCACATTTTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 28 GGCGCC G[GA]^CG[CT]C 247 GGCGTC AfaI 414 GTAC GT^AC 537 GTAC AvaII 144 GGACC G^G[AT]CC 255 GGACC BanII 322 GGGCCC G[GA]GC[CT]^C 526 GAGCCC BsaHI 28 GGCGCC G[GA]^CG[CT]C 247 GGCGTC BsmAI 451 GAGAC GTCTC(1/5) 471 GTCTC BsrBI 85 CCGCTC CCGCTC(-3/-3) 51 GAGCGG BsrI 25 ACTGG ACTGG(1/-1) 464 ACTGG DpnII 43 GATC ^GATC 132 GATC DraII 254 AGGACCC [GA]G^G[ACGT]CC[CT] 368 GGGGCCT EaeI 155 CGGCCA [CT]^GGCC[GA] 272 TGGCCA EcoO109I 254 AGGACCC [GA]G^G[ACGT]CC[CT] 368 GGGGCCT HaeII 28 GGCGCC [GA]GCGC^[CT] 236 GGCGCT HapII 151 CCGG C^CGG 573 CCGG Hin1I 28 GGCGCC G[GA]^CG[CT]C 247 GGCGTC HinfI 453 GACTC G^A[ACGT]TC 469 GAGTC HpaII 151 CCGG C^CGG 573 CCGG MaeII 79 ACGT A^CGT 497 ACGT MaeIII 298 GTGAC ^GT[ACGT]AC 461 GTGAC MboI 43 GATC ^GATC 132 GATC MspI 151 CCGG C^CGG 573 CCGG NdeII 43 GATC ^GATC 132 GATC NlaIII 67 CATG CATG^ 270 CATG PleI 453 GACTC GAGTC(4/5) 469 GAGTC RsaI 414 GTAC GT^AC 537 GTAC Sau3AI 43 GATC ^GATC 132 GATC TaqI 242 TCGA T^CGA 293 TCGA Tsp45I 298 GTGAC ^GT[GC]AC 461 GTGAC TthHB8I 242 TCGA T^CGA 293 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AccII AciI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I ItaI MboII MnlI MvaI MvnI MwoI NlaIV Sau96I ScrFI SfaNI TseI TspRI