################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AgeI AhdI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanI BbeI BbvCI BcgI BciVI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspMI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoRI EcoRV FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Alw44I 984 GTGCAC G^TGCAC AlwNI 1470 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1073 CCTCAGG CC^T[ACGT]AGG Aor51HI 559 AGCGCT AGC^GCT ApaLI 984 GTGCAC G^TGCAC BbrPI 135 CACGTG CAC^GTG BclI 752 TGATCA T^GATCA BfaI 337 CTAG C^TAG BglI 58 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 135 CACGTG [CT]AC^GT[GA] BsaI 1303 GGTCTC GGTCTC(1/5) BseRI 1243 CTCCTC GAGGAG(10/8) BsiEI 126 CGACCG CG[GA][CT]^CG Bsp1407I 452 TGTACA T^GTACA BspLU11I 1661 ACATGT A^CATGT BsrGI 452 TGTACA T^GTACA BssSI 1387 CTCGTG CACGAG(-5/-1) BstEII 1113 GGTGACC G^GT[ACGT]ACC BstPI 1113 GGTGACC G^GT[ACGT]ACC Bsu36I 1073 CCTCAGG CC^T[ACGT]AGG Eco47III 559 AGCGCT AGC^GCT Eco81I 1073 CCTCAGG CC^T[ACGT]AGG EcoO65I 1113 GGTGACC G^GT[ACGT]ACC EcoT22I 589 ATGCAT ATGCA^T FbaI 752 TGATCA T^GATCA HaeII 559 AGCGCT [GA]GCGC^[CT] MaeI 337 CTAG C^TAG MseI 1675 TTAA T^TAA NsiI 589 ATGCAT ATGCA^T PmaCI 135 CACGTG CAC^GTG PmlI 135 CACGTG CAC^GTG PvuII 1120 CAGCTG CAG^CTG SacI 1261 GAGCTC GAGCT^C SapI 1263 GCTCTTC GCTCTTC(1/4) SfiI 57 GGCCCCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 452 TGTACA T^GTACA StuI 1787 AGGCCT AGG^CCT TaqI 152 TCGA T^CGA Tru9I 1675 TTAA T^TAA Tsp509I 979 AATT ^AATT TthHB8I 152 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccI 718 GTCTAC GT^[AC][GT]AC 946 GTCTAC AflIII 134 ACACGT A^C[GA][CT]GT 1661 ACATGT BamHI 665 GGATCC G^GATCC 1465 GGATCC BanII 1261 GAGCTC G[GA]GC[CT]^C 1680 GGGCTC BglII 35 AGATCT A^GATCT 872 AGATCT BpmI 201 CTGGAG CTGGAG(16/14) 298 CTGGAG BsmAI 1304 GTCTC GTCTC(1/5) 1405 GTCTC BsrDI 1056 CATTGC GCAATG(2/0) 1269 CATTGC HgaI 777 GACGC GACGC(5/10) 1557 GACGC HincII 538 GTCAAC GT[CT]^[GA]AC 1429 GTCAAC HindII 538 GTCAAC GT[CT]^[GA]AC 1429 GTCAAC MaeII 136 ACGT A^CGT 197 ACGT NcoI 1568 CCATGG C^CATGG 1631 CCATGG PleI 841 GACTC GAGTC(4/5) 955 GACTC PpuMI 281 GGGTCCC [GA]G^G[AT]CC[CT] 901 GGGTCCC SfcI 528 CTACAG C^T[GA][CT]AG 948 CTACAG TfiI 1004 GATTC G^A[AT]TC 1436 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaI AvaII BalI BbsI BbvI BcnI BmyI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI RsaI Sau3AI Sau96I ScrFI SfaNI SmaI StyI TseI Tsp45I TspRI Van91I XcmI XhoII XmaCI XmaI