(Linear) (Six-base) MAPSORT of: KIEE4534.seq Check: 5734 from: 1 to: 1797 KIEE4534.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AccI GT'mk_AC Cuts at: 0 719 947 1797 Size: 719 228 850 Fragments arranged by size: 850 719 228 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1708 1797 Size: 1708 89 AfeI AGC'GCT Cuts at: 0 561 1797 Size: 561 1236 AflIII A'CryG_T Cuts at: 0 134 1661 1797 Size: 134 1527 136 Fragments arranged by size: 1527 136 134 AleI CACnn'nnGTG Cuts at: 0 477 1797 Size: 477 1320 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1393 1797 Size: 1393 404 AlwNI CAG_nnn'CTG Cuts at: 0 1475 1797 Size: 1475 322 ApaLI G'TGCA_C Cuts at: 0 984 1797 Size: 984 813 AvaI C'yCGr_G Cuts at: 0 784 916 1081 1645 1797 Size: 784 132 165 564 152 Fragments arranged by size: 784 564 165 152 132 BamHI G'GATC_C Cuts at: 0 665 1465 1797 Size: 665 800 332 Fragments arranged by size: 800 665 332 BanII G_rGCy'C Cuts at: 0 1265 1684 1797 Size: 1265 419 113 Fragments arranged by size: 1265 419 113 BbsI GAAGACnn'nnnn_ Cuts at: 0 1329 1619 1730 1797 Size: 1329 290 111 67 Fragments arranged by size: 1329 290 111 67 BclI T'GATC_A Cuts at: 0 752 1797 Size: 752 1045 BfrBI ATG'CAT Cuts at: 0 591 1797 Size: 591 1206 BglI GCCn_nnn'nGGC Cuts at: 0 64 1797 Size: 64 1733 BglII A'GATC_T Cuts at: 0 35 872 1797 Size: 35 837 925 Fragments arranged by size: 925 837 35 Bme1580I G_kGCm'C Cuts at: 0 988 1797 Size: 988 809 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 222 319 1797 Size: 222 97 1478 Fragments arranged by size: 1478 222 97 Bpu10I CC'TnA_GC Cuts at: 0 1751 1797 Size: 1751 46 BsaI GGTCTCn'nnnn_ Cuts at: 0 1309 1797 Size: 1309 488 BsaAI yAC'GTr Cuts at: 0 137 1797 Size: 137 1660 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 940 970 1797 Size: 940 30 827 Fragments arranged by size: 940 827 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1234 1797 Size: 1234 563 BseYI C'CCAG_C Cuts at: 0 891 1118 1173 1717 1797 Size: 891 227 55 544 80 Fragments arranged by size: 891 544 227 80 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 1179 1570 1797 Size: 121 1058 391 227 Fragments arranged by size: 1058 391 227 121 BsiEI CG_ry'CG Cuts at: 0 129 1797 Size: 129 1668 BsiHKAI G_wGCw'C Cuts at: 0 307 988 1265 1797 Size: 307 681 277 532 Fragments arranged by size: 681 532 307 277 Bsp1286I G_dGCh'C Cuts at: 0 307 988 1265 1684 1797 Size: 307 681 277 419 113 Fragments arranged by size: 681 419 307 277 113 BsrDI GCAATG_nn' Cuts at: 0 1055 1268 1797 Size: 1055 213 529 Fragments arranged by size: 1055 529 213 BsrGI T'GTAC_A Cuts at: 0 452 1797 Size: 452 1345 BssSI C'ACGA_G Cuts at: 0 1387 1797 Size: 1387 410 BstEII G'GTnAC_C Cuts at: 0 1113 1797 Size: 1113 684 BstXI CCAn_nnnn'nTGG Cuts at: 0 87 189 564 651 1797 Size: 87 102 375 87 1146 Fragments arranged by size: 1146 375 102 87 87 BstYI r'GATC_y Cuts at: 0 35 626 665 872 1465 1797 Size: 35 591 39 207 593 332 Fragments arranged by size: 593 591 332 207 39 35 Bsu36I CC'TnA_GG Cuts at: 0 1074 1797 Size: 1074 723 BtgI C'CryG_G Cuts at: 0 478 768 1568 1631 1797 Size: 478 290 800 63 166 Fragments arranged by size: 800 478 290 166 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 642 1797 Size: 642 1155 BtsI GCAGTG_nn' Cuts at: 0 472 1797 Size: 472 1325 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1502 1537 1797 Size: 1502 35 260 Fragments arranged by size: 1502 260 35 EaeI y'GGCC_r Cuts at: 0 23 65 634 641 653 1797 Size: 23 42 569 7 12 1144 Fragments arranged by size: 1144 569 42 23 12 7 EarI CTCTTCn'nnn_ Cuts at: 0 295 811 1042 1156 1270 1797 Size: 295 516 231 114 114 527 Fragments arranged by size: 527 516 295 231 114 114 EcoICRI GAG'CTC Cuts at: 0 1263 1797 Size: 1263 534 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 222 319 1708 1797 Size: 222 97 1389 89 Fragments arranged by size: 1389 222 97 89 EcoO109I rG'GnC_Cy Cuts at: 0 57 282 902 1078 1472 1797 Size: 57 225 620 176 394 325 Fragments arranged by size: 620 394 325 225 176 57 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 165 1797 Size: 165 1632 HaeII r_GCGC'y Cuts at: 0 563 1797 Size: 563 1234 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 995 1027 1342 1374 1797 Size: 995 32 315 32 423 Fragments arranged by size: 995 423 315 32 32 HincII GTy'rAC Cuts at: 0 540 1431 1797 Size: 540 891 366 Fragments arranged by size: 891 540 366 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 548 1443 1797 Size: 548 895 354 Fragments arranged by size: 895 548 354 MscI TGG'CCA Cuts at: 0 67 636 643 655 1797 Size: 67 569 7 12 1142 Fragments arranged by size: 1142 569 67 12 7 MslI CAynn'nnrTG Cuts at: 0 477 941 991 1797 Size: 477 464 50 806 Fragments arranged by size: 806 477 464 50 MspA1I CmG'CkG Cuts at: 0 515 632 1122 1797 Size: 515 117 490 675 Fragments arranged by size: 675 515 490 117 NcoI C'CATG_G Cuts at: 0 1568 1631 1797 Size: 1568 63 166 Fragments arranged by size: 1568 166 63 NsiI A_TGCA'T Cuts at: 0 593 1797 Size: 593 1204 NspI r_CATG'y Cuts at: 0 591 996 1665 1797 Size: 591 405 669 132 Fragments arranged by size: 669 591 405 132 PasI CC'CwG_GG Cuts at: 0 309 1797 Size: 309 1488 PciI A'CATG_T Cuts at: 0 1661 1797 Size: 1661 136 PflMI CCAn_nnn'nTGG Cuts at: 0 662 1458 1605 1707 1744 1797 Size: 662 796 147 102 37 53 Fragments arranged by size: 796 662 147 102 53 37 PmlI CAC'GTG Cuts at: 0 137 1797 Size: 137 1660 PpuMI rG'GwC_Cy Cuts at: 0 282 902 1797 Size: 282 620 895 Fragments arranged by size: 895 620 282 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 400 432 1797 Size: 400 32 1365 Fragments arranged by size: 1365 400 32 PvuII CAG'CTG Cuts at: 0 1122 1797 Size: 1122 675 SacI G_AGCT'C Cuts at: 0 1265 1797 Size: 1265 532 SanDI GG'GwC_CC Cuts at: 0 282 902 1797 Size: 282 620 895 Fragments arranged by size: 895 620 282 SapI GCTCTTCn'nnn_ Cuts at: 0 1270 1797 Size: 1270 527 SfcI C'TryA_G Cuts at: 0 528 948 1797 Size: 528 420 849 Fragments arranged by size: 849 528 420 SfiI GGCCn_nnn'nGGCC Cuts at: 0 64 1797 Size: 64 1733 SmaI CCC'GGG Cuts at: 0 786 1083 1647 1797 Size: 786 297 564 150 Fragments arranged by size: 786 564 297 150 StuI AGG'CCT Cuts at: 0 1789 1797 Size: 1789 8 StyI C'CwwG_G Cuts at: 0 86 791 1568 1631 1797 Size: 86 705 777 63 166 Fragments arranged by size: 777 705 166 86 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 834 1291 1797 Size: 834 457 506 Fragments arranged by size: 834 506 457 TatI w'GTAC_w Cuts at: 0 317 452 1797 Size: 317 135 1345 Fragments arranged by size: 1345 317 135 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 216 248 809 841 1797 Size: 216 32 561 32 956 Fragments arranged by size: 956 561 216 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 87 531 564 1062 1459 1797 Size: 87 444 33 498 397 338 Fragments arranged by size: 498 444 397 338 87 33 XmaI C'CCGG_G Cuts at: 0 784 1081 1645 1797 Size: 784 297 564 152 Fragments arranged by size: 784 564 297 152 Enzymes that do cut: AccI AcuI AfeI AflIII AleI AlfI AlwNI ApaLI AvaI BamHI BanII BbsI BclI BfrBI BglI BglII Bme1580I BpmI Bpu10I BsaI BsaAI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrDI BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI NsiI NspI PasI PciI PflMI PmlI PpuMI PsrI PvuII SacI SanDI SapI SfcI SfiI SmaI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BciVI BlpI BmgBI BmrI BmtI BplI BpuEI BsaBI BsaHI BsaWI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII BstAPI BstBI BstZ17I ClaI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI