################################## # NO CUT ENZYMES ( 216 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 22 enzymes) # ################################### AccI 131 GTCGAC GT^[AC][GT]AC AciI 49 GCGG CCGC(-3/-1) AspHI 207 GAGCAC G[AT]GC[AT]^C BbvCI 64 CCTCAGC CCTCAGC(-5/-2) BmyI 207 GAGCAC G[AGT]GC[ACT]^C BseRI 121 CTCCTC GAGGAG(10/8) BsiHKAI 207 GAGCAC G[AT]GC[AT]^C Bsp1286I 207 GAGCAC G[AGT]GC[ACT]^C HincII 131 GTCGAC GT[CT]^[GA]AC HindII 131 GTCGAC GT[CT]^[GA]AC HphI 116 TCACC GGTGA(8/7) MspA1I 47 CAGCGG C[AC]G^C[GT]G NlaIII 244 CATG CATG^ NlaIV 215 GGAACC GG[ACGT]^[ACGT]CC PleI 234 GACTC GAGTC(4/5) SalI 131 GTCGAC G^TCGAC SfaNI 85 GATGC GCATC(5/9) TaqI 132 TCGA T^CGA TfiI 3 GATTC G^A[AT]TC Tsp509I 108 AATT ^AATT TspRI 11 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 132 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BbvI 46 GCAGC GCAGC(8/12) 160 GCAGC BsaJI 53 CCCTGG C^C[ACGT][ACGT]GG 54 CCTGGG BsmAI 223 GAGAC GTCTC(1/5) 232 GAGAC BstF5I 90 CATCC GGATG(2/0) 84 GGATG Cfr13I 51 GGCCC G^G[ACGT]CC 201 GGGCC DdeI 65 CTCAG C^T[ACGT]AG 205 CTGAG FokI 90 CATCC GGATG(9/13) 84 GGATG HaeIII 51 GGCC GG^CC 202 GGCC HinfI 3 GATTC G^A[ACGT]TC 234 GACTC Sau96I 51 GGCCC G^G[ACGT]CC 201 GGGCC TseI 46 GCAGC G^C[AT]GC 160 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AluI BssKI BstNI EcoRII Fnu4HI ItaI MnlI MvaI ScrFI