(Circular) (Six-base) MAPSORT of: pFN21AE4459.seq Check: 67 from: 1 to: 6133 pFN21AE4459 6133 bp With 182 enzymes: SgfI * March 28, 2012 14:03 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3943 278 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3476 1937 Size: 1539 4594 Acc65I G'GTAC_C Cuts at: 3454 3454 Size: 6133 AclI AA'CG_TT Cuts at: 1563 4260 4633 1563 Size: 2697 373 3063 Fragments arranged by size: 3063 2697 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3075 3086 4196 5580 5848 1632 Size: 1443 11 1110 1384 268 1917 Fragments arranged by size: 1917 1443 1384 1110 268 11 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5936 AflIII A'CryG_T Cuts at: 1203 5032 6009 1203 Size: 3829 977 1327 Fragments arranged by size: 3829 1327 977 AgeI A'CCGG_T Cuts at: 4964 4964 Size: 6133 AhdI GACnn_n'nnGTC Cuts at: 4862 4862 Size: 6133 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6133 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2045 1184 Size: 861 5272 AlwNI CAG_nnn'CTG Cuts at: 3009 3876 5448 3009 Size: 867 1572 3694 Fragments arranged by size: 3694 1572 867 ApaLI G'TGCA_C Cuts at: 2212 4190 5346 2212 Size: 1978 1156 2999 Fragments arranged by size: 2999 1978 1156 ApoI r'AATT_y Cuts at: 1493 2321 3427 3442 3668 3820 5929 1493 Size: 828 1106 15 226 152 2109 1697 Fragments arranged by size: 2109 1697 1106 828 226 152 15 AseI AT'TA_AT Cuts at: 160 1032 4687 160 Size: 872 3655 1606 Fragments arranged by size: 3655 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 AvaI C'yCGr_G Cuts at: 1870 1943 2093 2174 2850 3458 1870 Size: 73 150 81 676 608 4545 Fragments arranged by size: 4545 676 608 150 81 73 BamHI G'GATC_C Cuts at: 3463 3830 3463 Size: 367 5766 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2075 2098 2121 3454 4909 Size: 325 217 627 288 23 23 1333 1455 Cuts at: 4909 618 Size: 1842 Fragments arranged by size: 1842 1455 1333 627 325 288 217 23 23 BanII G_rGCy'C Cuts at: 1391 2094 2268 3452 1391 Size: 703 174 1184 4072 Fragments arranged by size: 4072 1184 703 174 BbeI G_GCGC'C Cuts at: 2079 2102 2125 2079 Size: 23 23 6087 Fragments arranged by size: 6087 23 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3074 928 Size: 971 1175 3987 Fragments arranged by size: 3987 1175 971 BbvCI CC'TCA_GC Cuts at: 3274 3274 Size: 6133 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2137 2171 4324 4358 1903 Size: 34 200 34 2153 34 3678 Fragments arranged by size: 3678 2153 200 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4025 5235 4025 Size: 1210 4923 BclI T'GATC_A Cuts at: 1548 1548 Size: 6133 BfrBI ATG'CAT Cuts at: 2344 2344 Size: 6133 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4744 136 Size: 107 122 71 631 605 3072 1525 Fragments arranged by size: 3072 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2560 6128 2560 Size: 3568 2565 BlpI GC'TnA_GC Cuts at: 3032 3550 3032 Size: 518 5615 Bme1580I G_kGCm'C Cuts at: 1790 2050 2216 4194 5350 1790 Size: 260 166 1978 1156 2573 Fragments arranged by size: 2573 1978 1156 260 166 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6133 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2383 3085 3864 Size: 416 53 83 399 24 966 702 779 Cuts at: 3864 4822 442 Size: 958 1753 Fragments arranged by size: 1753 966 958 779 702 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6133 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2390 2390 Size: 6133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2994 3256 3264 3371 4793 1239 Size: 1755 262 8 107 1422 2579 Fragments arranged by size: 2579 1755 1422 262 107 8 Bpu10I CC'TnA_GC Cuts at: 2999 3274 2999 Size: 275 5858 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2504 3131 4258 5123 5421 2504 Size: 627 1127 865 298 3216 Fragments arranged by size: 3216 1127 865 627 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2681 2748 3141 4796 882 Size: 617 1182 67 393 1655 2219 Fragments arranged by size: 2219 1655 1182 617 393 67 BsaAI yAC'GTr Cuts at: 493 1204 2602 3924 493 Size: 711 1398 1322 2702 Fragments arranged by size: 2702 1398 1322 711 BsaBI GATnn'nnATC Cuts at: 6127 6127 Size: 6133 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2076 2099 2122 2298 Size: 53 83 186 936 543 23 23 176 Cuts at: 2298 2829 3940 4322 4957 275 Size: 531 1111 382 635 1451 Fragments arranged by size: 1451 1111 936 635 543 531 382 186 176 83 53 23 23 BsaWI w'CCGG_w Cuts at: 2568 4566 4964 5238 5385 5796 2568 Size: 1998 398 274 147 411 2905 Fragments arranged by size: 2905 1998 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2135 2165 2745 2775 2782 Size: 30 165 30 732 30 580 30 7 Cuts at: 2782 2801 2812 2831 3077 3107 1178 Size: 19 11 19 246 30 4204 Fragments arranged by size: 4204 732 580 246 165 30 30 30 30 30 19 19 11 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2766 2806 3141 3392 1186 Size: 1580 40 335 251 3927 Fragments arranged by size: 3927 1580 335 251 40 BseYI C'CCAG_C Cuts at: 3332 5336 5832 3332 Size: 2004 496 3633 Fragments arranged by size: 3633 2004 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5560 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3583 4344 5372 1540 Size: 275 186 1582 761 1028 2301 Fragments arranged by size: 2301 1582 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2216 2268 2803 4194 4279 5350 2216 Size: 52 535 1391 85 1071 2999 Fragments arranged by size: 2999 1391 1071 535 85 52 BsmI GAATG_Cn' Cuts at: 2454 3178 3644 3737 2454 Size: 724 466 93 4850 Fragments arranged by size: 4850 724 466 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2508 2777 2508 Size: 269 5864 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2094 2216 2268 2803 3452 4194 Size: 399 260 44 122 52 535 649 742 Cuts at: 4194 4279 5350 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 649 535 399 260 122 85 52 44 BspEI T'CCGG_A Cuts at: 5796 5796 Size: 6133 BspHI T'CATG_A Cuts at: 2803 4022 5752 2803 Size: 1219 1730 3184 Fragments arranged by size: 3184 1730 1219 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3488 844 Size: 2644 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4020 1394 Size: 429 2197 3507 Fragments arranged by size: 3507 2197 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2551 4628 4802 65 Size: 1183 1303 2077 174 1396 Fragments arranged by size: 2077 1396 1303 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2124 4777 4964 1700 Size: 110 314 2653 187 2869 Fragments arranged by size: 2869 2653 314 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6133 BssHII G'CGCG_C Cuts at: 1925 2025 1925 Size: 100 6033 BssSI C'ACGA_G Cuts at: 2192 4193 5205 2192 Size: 2001 1012 3120 Fragments arranged by size: 3120 2001 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2054 794 Size: 1260 4873 BstBI TT'CG_AA Cuts at: 4937 4937 Size: 6133 BstEII G'GTnAC_C Cuts at: 1184 3158 1184 Size: 1974 4159 BstXI CCAn_nnnn'nTGG Cuts at: 2309 3187 2309 Size: 878 5255 BstYI r'GATC_y Cuts at: 1972 2560 3463 3830 4215 4232 5684 5770 5782 Size: 588 903 367 385 17 1452 86 12 Cuts at: 5782 6128 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 903 588 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2103 2746 3024 3186 513 Size: 552 939 99 643 278 162 3460 Fragments arranged by size: 3460 939 643 552 278 162 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5394 Fragments arranged by size: 5394 683 56 BtsI GCAGTG_nn' Cuts at: 2216 3649 4443 4463 2216 Size: 1433 794 20 3886 Fragments arranged by size: 3886 1433 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6133 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3288 3323 529 Size: 35 2724 35 3339 Fragments arranged by size: 3339 2724 35 35 DraI TTT'AAA Cuts at: 2326 3426 3437 2326 Size: 1100 11 5022 Fragments arranged by size: 5022 1100 11 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5140 809 Size: 507 3824 1802 Fragments arranged by size: 3824 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2763 2874 Size: 54 1425 50 275 18 177 756 111 Cuts at: 2874 2894 3580 4469 6098 8 Size: 20 686 889 1629 43 Fragments arranged by size: 1629 1425 889 756 686 275 177 111 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 3580 1537 Size: 275 1768 4090 Fragments arranged by size: 4090 1768 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2866 3051 3369 4063 1353 Size: 1513 185 318 694 3423 Fragments arranged by size: 3423 1513 694 318 185 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4720 5094 5240 387 Size: 1064 138 3131 374 146 1280 Fragments arranged by size: 3131 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2266 2266 Size: 6133 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2994 3075 3086 3256 3264 3371 4196 Size: 393 1362 81 11 170 8 107 825 Cuts at: 4196 4793 5580 5848 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1362 825 787 597 393 268 170 107 81 11 8 EcoRI G'AATT_C Cuts at: 3442 3820 3442 Size: 378 5755 FspI TGC'GCA Cuts at: 4639 4639 Size: 6133 HaeII r_GCGC'y Cuts at: 1678 2079 2102 2125 2192 5280 1678 Size: 401 23 23 67 3088 2531 Fragments arranged by size: 3088 2531 401 67 23 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 3197 3229 4774 Size: 32 135 32 563 32 1030 32 1545 Cuts at: 4774 4806 4848 4880 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1545 1030 563 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2434 3477 3723 669 Size: 988 281 496 1043 246 3079 Fragments arranged by size: 3079 1043 988 496 281 246 HindIII A'AGCT_T Cuts at: 748 2675 2842 748 Size: 1927 167 4039 Fragments arranged by size: 4039 1927 167 HpaI GTT'AAC Cuts at: 3723 3723 Size: 6133 KasI G'GCGC_C Cuts at: 2075 2098 2121 2075 Size: 23 23 6087 Fragments arranged by size: 6087 23 23 KpnI G_GTAC'C Cuts at: 3458 3458 Size: 6133 MfeI C'AATT_G Cuts at: 2427 3165 3732 2427 Size: 738 567 4828 Fragments arranged by size: 4828 738 567 MluI A'CGCG_T Cuts at: 6009 6009 Size: 6133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2310 2341 5247 5431 1305 Size: 1005 31 2906 184 2007 Fragments arranged by size: 2906 2007 1005 184 31 MscI TGG'CCA Cuts at: 10 64 1489 1832 2765 10 Size: 54 1425 343 933 3378 Fragments arranged by size: 3378 1425 933 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2349 3185 4091 4450 4609 518 Size: 648 1183 836 906 359 159 2042 Fragments arranged by size: 2042 1183 906 836 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2105 3549 4226 5374 5619 1606 Size: 499 1444 677 1148 245 2120 Fragments arranged by size: 2120 1444 1148 677 499 245 NaeI GCC'GGC Cuts at: 2126 2126 Size: 6133 NarI GG'CG_CC Cuts at: 2076 2099 2122 2076 Size: 23 23 6087 Fragments arranged by size: 6087 23 23 NcoI C'CATG_G Cuts at: 513 1065 2004 2746 513 Size: 552 939 742 3900 Fragments arranged by size: 3900 939 742 552 NdeI CA'TA_TG Cuts at: 387 2776 387 Size: 2389 3744 NgoMIV G'CCGG_C Cuts at: 2124 2124 Size: 6133 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6133 NotI GC'GGCC_GC Cuts at: 3580 3580 Size: 6133 NsiI A_TGCA'T Cuts at: 2346 2346 Size: 6133 NspI r_CATG'y Cuts at: 1132 1229 3491 5036 1132 Size: 97 2262 1545 2229 Fragments arranged by size: 2262 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2551 1348 Size: 1203 4930 PciI A'CATG_T Cuts at: 5032 5032 Size: 6133 PflMI CCAn_nnn'nTGG Cuts at: 1108 2845 1108 Size: 1737 4396 PmeI GTTT'AAAC Cuts at: 3437 3437 Size: 6133 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4202 4234 5715 5747 4202 Size: 32 1481 32 4588 Fragments arranged by size: 4588 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2429 1613 Size: 816 5317 PsiI TTA'TAA Cuts at: 3703 3703 Size: 6133 PstI C_TGCA'G Cuts at: 830 2136 2211 3485 830 Size: 1306 75 1274 3478 Fragments arranged by size: 3478 1306 1274 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6133 SacI G_AGCT'C Cuts at: 2268 2268 Size: 6133 SacII CC_GC'GG Cuts at: 2106 2106 Size: 6133 SalI G'TCGA_C Cuts at: 1936 3475 1936 Size: 1539 4594 SbfI CC_TGCA'GG Cuts at: 3485 3485 Size: 6133 ScaI AGT'ACT Cuts at: 4381 6093 4381 Size: 1712 4421 SfcI C'TryA_G Cuts at: 826 1045 2132 2207 3481 4616 5297 5488 826 Size: 219 1087 75 1274 1135 681 191 1471 Fragments arranged by size: 1471 1274 1135 1087 681 219 191 75 SfoI GGC'GCC Cuts at: 2077 2100 2123 2077 Size: 23 23 6087 Fragments arranged by size: 6087 23 23 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 SgrAI Cr'CCGG_yG Cuts at: 1700 2124 1700 Size: 424 5709 SmaI CCC'GGG Cuts at: 1872 2095 2852 3460 1872 Size: 223 757 608 4545 Fragments arranged by size: 4545 757 608 223 SmlI C'TyrA_G Cuts at: 820 1017 1943 2519 3110 4237 5138 5400 820 Size: 197 926 576 591 1127 901 262 1553 Fragments arranged by size: 1553 1127 926 901 591 576 262 197 SnaBI TAC'GTA Cuts at: 493 3924 493 Size: 3431 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6133 SphI G_CATG'C Cuts at: 1132 3491 1132 Size: 2359 3774 SrfI GCCC'GGGC Cuts at: 2852 2852 Size: 6133 SspI AAT'ATT Cuts at: 5 52 4057 5 Size: 47 4005 2081 Fragments arranged by size: 4005 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6133 StyI C'CwwG_G Cuts at: 513 1065 2004 2691 2746 3572 513 Size: 552 939 687 55 826 3074 Fragments arranged by size: 3074 939 826 687 552 55 SwaI ATTT'AAAT Cuts at: 2326 2326 Size: 6133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4160 4177 4330 4515 4960 325 Size: 1255 2580 17 153 185 445 1498 Fragments arranged by size: 2580 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4379 6091 96 Size: 274 80 33 51 1443 2402 1712 138 Fragments arranged by size: 2402 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2745 2777 2782 2814 6093 6125 2745 Size: 32 5 32 3279 32 2753 Fragments arranged by size: 3279 2753 32 32 32 5 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6133 XbaI T'CTAG_A Cuts at: 3469 3469 Size: 6133 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3184 3184 Size: 6133 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6133 XmaI C'CCGG_G Cuts at: 1870 2093 2850 3458 1870 Size: 223 757 608 4545 Fragments arranged by size: 4545 757 608 223 XmnI GAAnn'nnTTC Cuts at: 4262 4262 Size: 6133 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3941 276 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI