################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AocI 1231 CCTGAGG CC^T[ACGT]AGG ApaI 118 GGGCCC GGGCC^C BanI 1026 GGCACC G^G[CT][GA]CC BbsI 301 GTCTTC GAAGAC(2/6) BbvCI 361 GCTGAGG CCTCAGC(-5/-2) BbvI 886 GCAGC GCAGC(8/12) BclI 266 TGATCA T^GATCA BpmI 877 CTGGAG CTGGAG(16/14) BpuAI 301 GTCTTC GAAGAC(2/6) BsaI 166 GGTCTC GGTCTC(1/5) BspHI 1343 TCATGA T^CATGA BstAPI 984 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 840 TTCGAA TT^CGAA BstXI 1213 CCAAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1231 CCTGAGG CC^T[ACGT]AGG DraIII 183 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1046 CCGTGG C^C[GA][CT]GG Eco81I 1231 CCTGAGG CC^T[ACGT]AGG FauI 483 GCGGG CCCGC(4/6) FbaI 266 TGATCA T^GATCA Fnu4HI 886 GCAGC GC^[ACGT]GC HincII 230 GTCAAC GT[CT]^[GA]AC HindII 230 GTCAAC GT[CT]^[GA]AC ItaI 886 GCAGC GC^[ACGT]GC MaeIII 508 GTCAC ^GT[ACGT]AC MwoI 984 GCAGAAACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspV 840 TTCGAA TT^CGAA PpuMI 76 GGGACCC [GA]G^G[AT]CC[CT] PvuII 817 CAGCTG CAG^CTG RcaI 1343 TCATGA T^CATGA SacI 880 GAGCTC GAGCT^C SapI 1198 GCTCTTC GCTCTTC(1/4) ScaI 1217 AGTACT AGT^ACT SfcI 534 CTACAG C^T[GA][CT]AG SfuI 840 TTCGAA TT^CGAA TseI 886 GCAGC G^C[AT]GC Tsp45I 508 GTCAC ^GT[GC]AC XcmI 1166 CCAAAGGGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BalI 245 TGGCCA TGG^CCA 962 TGGCCA BanII 118 GGGCCC G[GA]GC[CT]^C 880 GAGCTC BfaI 280 CTAG C^TAG 805 CTAG BsaJI 115 CCAGGG C^C[ACGT][ACGT]GG 1046 CCGTGG BseRI 61 CTCCTC GAGGAG(10/8) 1234 GAGGAG BsmFI 76 GGGAC GGGAC(10/14) 855 GTCCC BsrDI 392 CATTGC GCAATG(2/0) 444 CATTGC EaeI 245 TGGCCA [CT]^GGCC[GA] 962 TGGCCA EarI 1199 CTCTTC CTCTTC(1/4) 378 GAAGAG EcoRI 110 GAATTC G^AATTC 837 GAATTC Ksp632I 1199 CTCTTC CTCTTC(1/4) 378 GAAGAG MaeI 280 CTAG C^TAG 805 CTAG MaeII 220 ACGT A^CGT 263 ACGT MluNI 245 TGGCCA TGG^CCA 962 TGGCCA MscI 245 TGGCCA TGG^CCA 962 TGGCCA MspA1I 414 CAGCGG C[AC]G^C[GT]G 817 CAGCTG SfaNI 1006 GATGC GCATC(5/9) 1042 GATGC TfiI 1185 GATTC G^A[AT]TC 1477 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BmyI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FokI HaeIII HinfI HphI MboI MboII MflI MnlI MseI MslI MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SmlI TaqI Tru9I Tsp509I TspRI TthHB8I XhoII