Restriction map for
pFN21AE6434
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoT22I
FbaI FseI FspI HaeII HincII
HindII HpaI KasI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NarI NcoI
NdeI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I XbaI
XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 752 GGTACC G^GTACC
AccI 1177 GTCTAC GT^[AC][GT]AC
AcsI 367 GAATTC [GA]^AATT[CT]
AflIII 1121 ACGTGT A^C[GA][CT]GT
ApaI 40 GGGCCC GGGCC^C
ApoI 367 GAATTC [GA]^AATT[CT]
Asp700I 989 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 752 GGTACC G^GTACC
BbvCI 816 CCTCAGC CCTCAGC(-5/-2)
BsaAI 535 TACGTG [CT]AC^GT[GA]
BsaI 1564 GAGACC GGTCTC(1/5)
BsmBI 1540 GAGACG CGTCTC(1/5)
BsrDI 381 CATTGC GCAATG(2/0)
BstYI 402 GGATCT [GA]^GATC[CT]
CfoI 1191 GCGC GCG^C
DraII 40 GGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 1615 GAAGAG CTCTTC(1/4)
EcoO109I 40 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 367 GAATTC G^AATTC
EcoRV 488 GATATC GAT^ATC
EcoT14I 861 CCAAGG C^C[AT][AT]GG
FauI 602 CCCGC CCCGC(4/6)
HhaI 1191 GCGC GCG^C
HinP1I 1191 GCGC G^CGC
KpnI 752 GGTACC GGTAC^C
Ksp632I 1615 GAAGAG CTCTTC(1/4)
MflI 402 GGATCT [GA]^GATC[CT]
NheI 783 GCTAGC G^CTAGC
PflMI 1572 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 566 CTGCAG CTGCA^G
SfcI 566 CTGCAG C^T[GA][CT]AG
SgrAI 456 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 209 CCCGGG CCC^GGG
StyI 861 CCAAGG C^C[AT][AT]GG
Tsp509I 368 AATT ^AATT
Van91I 1572 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 402 GGATCT [GA]^GATC[CT]
XmaCI 209 CCCGGG C^CCGGG
XmaI 209 CCCGGG C^CCGGG
XmnI 989 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 515 GGCGTC G[GA]^CG[CT]C
1202 GACGCC
AlwI 402 GGATC GGATC(4/5)
1499 GGATC
AlwNI 231 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
819 CAGCCTCTG
AvaI 80 CCCGAG C^[CT]CG[GA]G
209 CCCGGG
AvaII 984 GGTCC G^G[AT]CC
1635 GGTCC
BanII 40 GGGCCC G[GA]GC[CT]^C
1428 GGGCTC
BbsI 989 GAAGAC GAAGAC(2/6)
1604 GTCTTC
BglI 1438 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1504 GCCCTGCTGGC
BlpI 228 GCTCAGC GC^T[ACGT]AGC
617 GCTCAGC
Bpu1102I 228 GCTCAGC GC^T[ACGT]AGC
617 GCTCAGC
BpuAI 989 GAAGAC GAAGAC(2/6)
1604 GTCTTC
BsaHI 515 GGCGTC G[GA]^CG[CT]C
1202 GACGCC
BseRI 372 CTCCTC GAGGAG(10/8)
10 GAGGAG
BsmAI 1540 GAGAC GTCTC(1/5)
1564 GAGAC
BsoBI 80 CCCGAG C^[CT]CG[GA]G
209 CCCGGG
BstXI 113 CCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1166 CCAAGCTGCTGG
CelII 228 GCTCAGC GC^T[ACGT]AGC
617 GCTCAGC
DpnII 403 GATC ^GATC
1500 GATC
EaeI 919 CGGCCA [CT]^GGCC[GA]
965 TGGCCG
Hin1I 515 GGCGTC G[GA]^CG[CT]C
1202 GACGCC
HindIII 1316 AAGCTT A^AGCTT
1483 AAGCTT
MaeIII 790 GTCAC ^GT[ACGT]AC
1473 GTCAC
MboI 403 GATC ^GATC
1500 GATC
MspA1I 1135 CAGCTG C[AC]G^C[GT]G
1555 CAGCTG
NaeI 1418 GCCGGC GCC^GGC
1516 GCCGGC
NdeII 403 GATC ^GATC
1500 GATC
NgoMIV 1418 GCCGGC G^CCGGC
1516 GCCGGC
NlaIII 868 CATG CATG^
1626 CATG
PvuII 1135 CAGCTG CAG^CTG
1555 CAGCTG
Sau3AI 403 GATC ^GATC
1500 GATC
Tsp45I 790 GTCAC ^GT[GC]AC
1473 GTCAC
XcmI 269 CCAACACCACGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1568 CCATCCAGGACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AspHI BanI
BbvI BcnI BfaI BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI Cac8I Cfr10I
Cfr13I DdeI DsaI EcoRII Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI MaeI MaeII
MboII MnlI MslI MspI MvaI
MwoI NciI NlaIV PleI RsaI
Sau96I ScrFI SfaNI TseI TspRI