(Circular) (Six-base) MAPSORT of: pFN21AE6434.seq Check: 2542 from: 1 to: 6349 pFN21AE6434 6349 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2020 2020 Size: 6349 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4159 278 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3183 3692 1937 Size: 1246 509 4594 Fragments arranged by size: 4594 1246 509 Acc65I G'GTAC_C Cuts at: 2757 3670 2757 Size: 913 5436 AclI AA'CG_TT Cuts at: 1563 3648 4476 4849 1563 Size: 2085 828 373 3063 Fragments arranged by size: 3063 2085 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2161 2929 3348 3351 3596 4412 5796 6064 Size: 529 768 419 3 245 816 1384 268 Cuts at: 6064 1632 Size: 1917 Fragments arranged by size: 1917 1384 816 768 529 419 268 245 3 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6152 AflIII A'CryG_T Cuts at: 1203 3126 5248 6225 1203 Size: 1923 2122 977 1327 Fragments arranged by size: 2122 1923 1327 977 AgeI A'CCGG_T Cuts at: 5180 5180 Size: 6349 AhdI GACnn_n'nnGTC Cuts at: 5078 5078 Size: 6349 AleI CACnn'nnGTG Cuts at: 1166 2148 1166 Size: 982 5367 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3281 1184 Size: 2097 4252 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2973 3005 2973 Size: 32 6317 AlwNI CAG_nnn'CTG Cuts at: 2241 2829 4092 5664 2241 Size: 588 1263 1572 2926 Fragments arranged by size: 2926 1572 1263 588 ApaI G_GGCC'C Cuts at: 2049 2049 Size: 6349 ApaLI G'TGCA_C Cuts at: 4406 5562 4406 Size: 1156 5193 ApoI r'AATT_y Cuts at: 1493 2372 3658 3884 4036 6145 1493 Size: 879 1286 226 152 2109 1697 Fragments arranged by size: 2109 1697 1286 879 226 152 AseI AT'TA_AT Cuts at: 160 1032 4903 160 Size: 872 3871 1606 Fragments arranged by size: 3871 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 AvaI C'yCGr_G Cuts at: 1870 1943 2085 2214 3674 1870 Size: 73 142 129 1460 4545 Fragments arranged by size: 4545 1460 142 129 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2530 2563 2530 Size: 33 6316 BamHI G'GATC_C Cuts at: 3679 4046 3679 Size: 367 5982 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2162 2757 2836 3524 3670 Size: 325 217 627 375 595 79 688 146 Cuts at: 3670 5125 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 688 627 595 375 325 217 146 79 BanII G_rGCy'C Cuts at: 1391 2049 3437 3668 1391 Size: 658 1388 231 4072 Fragments arranged by size: 4072 1388 658 231 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3001 3602 928 Size: 971 1102 601 3675 Fragments arranged by size: 3675 1102 971 601 BbvCI CC'TCA_GC Cuts at: 2822 2822 Size: 6349 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4540 4574 1903 Size: 34 2603 34 3678 Fragments arranged by size: 3678 2603 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4241 5451 4241 Size: 1210 5139 BclI T'GATC_A Cuts at: 1548 1548 Size: 6349 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3449 3515 4960 Size: 107 122 71 631 605 1777 66 1445 Cuts at: 4960 136 Size: 1525 Fragments arranged by size: 1777 1525 1445 631 605 122 107 71 66 BglII A'GATC_T Cuts at: 6344 6344 Size: 6349 BlpI GC'TnA_GC Cuts at: 2234 2623 3766 2234 Size: 389 1143 4817 Fragments arranged by size: 4817 1143 389 Bme1580I G_kGCm'C Cuts at: 1790 2049 2155 2167 2340 2812 3242 3601 4410 Size: 259 106 12 173 472 430 359 809 Cuts at: 4410 5566 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 809 472 430 359 259 173 106 12 BmgBI CAC'GTC Cuts at: 1318 3583 1318 Size: 2265 4084 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2395 4080 5038 Size: 416 53 83 399 24 978 1685 958 Cuts at: 5038 442 Size: 1753 Fragments arranged by size: 1753 1685 978 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2792 1056 Size: 1736 4613 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2219 2336 2681 2687 2721 3313 5009 Size: 806 174 117 345 6 34 592 1696 Cuts at: 5009 1239 Size: 2579 Fragments arranged by size: 2579 1696 806 592 345 174 117 34 6 Bpu10I CC'TnA_GC Cuts at: 2708 2822 2708 Size: 114 6235 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4474 5339 5637 4474 Size: 865 298 5186 Fragments arranged by size: 5186 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3563 5012 882 Size: 617 2064 1449 2219 Fragments arranged by size: 2219 2064 1449 617 BsaAI yAC'GTr Cuts at: 493 1204 2542 4140 493 Size: 711 1338 1598 2702 Fragments arranged by size: 2702 1598 1338 711 BsaBI GATnn'nnATC Cuts at: 6343 6343 Size: 6349 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2521 3208 4156 4538 Size: 53 83 186 936 988 687 948 382 Cuts at: 4538 5173 275 Size: 635 1451 Fragments arranged by size: 1451 988 948 936 687 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4782 5180 5454 5601 6012 4782 Size: 398 274 147 411 5119 Fragments arranged by size: 5119 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2654 2684 3015 3045 1178 Size: 30 165 30 1251 30 331 30 4482 Fragments arranged by size: 4482 1251 331 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2368 1186 Size: 844 338 5167 Fragments arranged by size: 5167 844 338 BseYI C'CCAG_C Cuts at: 2352 2557 3115 3235 3427 5552 6048 2352 Size: 205 558 120 192 2125 496 2653 Fragments arranged by size: 2653 2125 558 496 205 192 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 2832 3396 1163 Size: 573 351 745 564 4116 Fragments arranged by size: 4116 745 573 564 351 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3799 4560 5588 1540 Size: 275 186 1798 761 1028 2301 Fragments arranged by size: 2301 1798 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2688 3299 3387 4410 4495 5566 2688 Size: 611 88 1023 85 1071 3471 Fragments arranged by size: 3471 1071 1023 611 88 85 BsmI GAATG_Cn' Cuts at: 3860 3953 3860 Size: 93 6256 BsmBI CGTCTCn'nnnn_ Cuts at: 3539 3539 Size: 6349 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2155 2167 2340 2688 2812 3242 Size: 399 259 106 12 173 348 124 430 Cuts at: 3242 3299 3387 3437 3601 3668 4410 4495 5566 Size: 57 88 50 164 67 742 85 1071 Cuts at: 5566 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 430 399 348 259 173 164 124 106 88 85 67 57 50 12 BspEI T'CCGG_A Cuts at: 6012 6012 Size: 6349 BspHI T'CATG_A Cuts at: 4238 5968 4238 Size: 1730 4619 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2020 2335 3628 3704 844 Size: 1176 315 1293 76 3489 Fragments arranged by size: 3489 1293 1176 315 76 BsrBI CCG'CTC Cuts at: 1394 1823 4236 1394 Size: 429 2413 3507 Fragments arranged by size: 3507 2413 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2385 4844 5018 65 Size: 1183 1137 2459 174 1396 Fragments arranged by size: 2459 1396 1183 1137 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2462 3423 3521 4993 5180 1700 Size: 110 652 961 98 1472 187 2869 Fragments arranged by size: 2869 1472 961 652 187 110 98 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6349 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6349 BssSI C'ACGA_G Cuts at: 4409 5421 4409 Size: 1012 5337 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6349 BstBI TT'CG_AA Cuts at: 5153 5153 Size: 6349 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6349 BstXI CCAn_nnnn'nTGG Cuts at: 2125 3178 2125 Size: 1053 5296 BstYI r'GATC_y Cuts at: 1972 2407 3679 4046 4431 4448 5900 5986 5998 Size: 435 1272 367 385 17 1452 86 12 Cuts at: 5998 6344 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1272 435 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2305 2694 3026 513 Size: 552 939 301 389 332 3836 Fragments arranged by size: 3836 939 552 389 332 301 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2791 487 Size: 683 56 1565 4045 Fragments arranged by size: 4045 1565 683 56 BtsI GCAGTG_nn' Cuts at: 2069 2099 3230 3315 3345 3479 3865 4659 4679 Size: 30 1131 85 30 134 386 794 20 Cuts at: 4679 2069 Size: 3739 Fragments arranged by size: 3739 1131 794 386 134 85 30 30 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6314 DraI TTT'AAA Cuts at: 3653 3653 Size: 6349 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5356 809 Size: 507 4040 1802 Fragments arranged by size: 4040 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2924 2970 3796 Size: 54 1425 50 275 18 1094 46 826 Cuts at: 3796 4685 6314 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1094 889 826 275 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3796 1537 Size: 275 1984 4090 Fragments arranged by size: 4090 1984 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3615 4279 1353 Size: 2262 664 3423 Fragments arranged by size: 3423 2262 664 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4936 5310 5456 387 Size: 1064 138 3347 374 146 1280 Fragments arranged by size: 3347 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2161 2219 2336 2681 2687 2721 Size: 393 413 116 58 117 345 6 34 Cuts at: 2721 2929 3313 3348 3351 3596 4412 5009 5796 Size: 208 384 35 3 245 816 597 787 Cuts at: 5796 6064 1239 Size: 268 1524 Fragments arranged by size: 1524 816 787 597 413 393 384 345 268 245 208 117 116 58 35 34 6 3 EcoO109I rG'GnC_Cy Cuts at: 2046 2046 Size: 6349 EcoRI G'AATT_C Cuts at: 2372 3658 4036 2372 Size: 1286 378 4685 Fragments arranged by size: 4685 1286 378 EcoRV GAT'ATC Cuts at: 2495 2495 Size: 6349 FspI TGC'GCA Cuts at: 4855 4855 Size: 6349 HaeII r_GCGC'y Cuts at: 1678 5496 1678 Size: 3818 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3198 3230 3272 3304 4990 Size: 32 135 32 1626 32 42 32 1686 Cuts at: 4990 5022 5064 5096 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1686 1626 135 42 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3693 3939 669 Size: 988 281 1755 246 3079 Fragments arranged by size: 3079 1755 988 281 246 HindIII A'AGCT_T Cuts at: 748 3321 3488 748 Size: 2573 167 3609 Fragments arranged by size: 3609 2573 167 HpaI GTT'AAC Cuts at: 3939 3939 Size: 6349 KpnI G_GTAC'C Cuts at: 2761 3674 2761 Size: 913 5436 MfeI C'AATT_G Cuts at: 3948 3948 Size: 6349 MluI A'CGCG_T Cuts at: 6225 6225 Size: 6349 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2077 2526 3058 5463 5647 1305 Size: 772 449 532 2405 184 2007 Fragments arranged by size: 2405 2007 772 532 449 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4527 Fragments arranged by size: 4527 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2148 2187 2648 2651 4307 4666 4825 Size: 648 982 39 461 3 1656 359 159 Cuts at: 4825 518 Size: 2042 Fragments arranged by size: 2042 1656 982 648 461 359 159 39 3 MspA1I CmG'CkG Cuts at: 1606 3142 3562 3765 4442 5590 5835 1606 Size: 1536 420 203 677 1148 245 2120 Fragments arranged by size: 2120 1536 1148 677 420 245 203 NaeI GCC'GGC Cuts at: 3425 3523 3425 Size: 98 6251 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4858 Fragments arranged by size: 4858 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6349 NgoMIV G'CCGG_C Cuts at: 3423 3521 3423 Size: 98 6251 NheI G'CTAG_C Cuts at: 1052 2788 1052 Size: 1736 4613 NotI GC'GGCC_GC Cuts at: 3796 3796 Size: 6349 NspI r_CATG'y Cuts at: 1132 1229 3707 5252 1132 Size: 97 2478 1545 2229 Fragments arranged by size: 2478 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6349 PciI A'CATG_T Cuts at: 5248 5248 Size: 6349 PflMI CCAn_nnn'nTGG Cuts at: 1108 3583 1108 Size: 2475 3874 PfoI T'CCnGG_A Cuts at: 3290 3576 3290 Size: 286 6063 PmeI GTTT'AAAC Cuts at: 3653 3653 Size: 6349 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4418 4450 5931 5963 4418 Size: 32 1481 32 4804 Fragments arranged by size: 4804 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6349 PsiI TTA'TAA Cuts at: 3919 3919 Size: 6349 PspOMI G'GGCC_C Cuts at: 2045 2045 Size: 6349 PstI C_TGCA'G Cuts at: 830 2575 3701 830 Size: 1745 1126 3478 Fragments arranged by size: 3478 1745 1126 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6349 PvuII CAG'CTG Cuts at: 3142 3562 3142 Size: 420 5929 SalI G'TCGA_C Cuts at: 1936 3691 1936 Size: 1755 4594 SbfI CC_TGCA'GG Cuts at: 3701 3701 Size: 6349 ScaI AGT'ACT Cuts at: 4597 6309 4597 Size: 1712 4637 SfcI C'TryA_G Cuts at: 826 1045 2571 3697 4832 5513 5704 826 Size: 219 1526 1126 1135 681 191 1471 Fragments arranged by size: 1526 1471 1135 1126 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 SgrAI Cr'CCGG_yG Cuts at: 1700 2462 1700 Size: 762 5587 SmaI CCC'GGG Cuts at: 1872 2216 3676 1872 Size: 344 1460 4545 Fragments arranged by size: 4545 1460 344 SmlI C'TyrA_G Cuts at: 820 1017 1943 4453 5354 5616 820 Size: 197 926 2510 901 262 1553 Fragments arranged by size: 2510 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4140 493 Size: 3647 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6349 SphI G_CATG'C Cuts at: 1132 3707 1132 Size: 2575 3774 SspI AAT'ATT Cuts at: 5 52 4273 5 Size: 47 4221 2081 Fragments arranged by size: 4221 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6349 StyI C'CwwG_G Cuts at: 513 1065 2004 2866 3788 513 Size: 552 939 862 922 3074 Fragments arranged by size: 3074 939 922 862 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2824 4376 4393 4546 4731 5176 325 Size: 1255 1244 1552 17 153 185 445 1498 Fragments arranged by size: 1552 1498 1255 1244 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4595 6307 96 Size: 274 80 33 51 1443 2618 1712 138 Fragments arranged by size: 2618 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2091 2123 2399 2431 2654 2686 3015 3047 3455 Size: 32 276 32 223 32 329 32 408 Cuts at: 3455 3487 6309 6341 2091 Size: 32 2822 32 2099 Fragments arranged by size: 2822 2099 408 329 276 223 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6349 XbaI T'CTAG_A Cuts at: 3685 3685 Size: 6349 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2281 3580 2281 Size: 1299 5050 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6349 XmaI C'CCGG_G Cuts at: 1870 2214 3674 1870 Size: 344 1460 4545 Fragments arranged by size: 4545 1460 344 XmnI GAAnn'nnTTC Cuts at: 2998 4478 2998 Size: 1480 4869 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4157 276 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI