(Circular) (Six-base) MAPSORT of: pFN21AB6214.seq Check: 2409 from: 1 to: 6880 pFN21AB6214 6880 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4690 278 Size: 53 83 186 936 3154 2468 Fragments arranged by size: 3154 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4223 1937 Size: 2286 4594 Acc65I G'GTAC_C Cuts at: 3373 4201 3373 Size: 828 6052 AclI AA'CG_TT Cuts at: 1563 5007 5380 1563 Size: 3444 373 3063 Fragments arranged by size: 3444 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2264 2460 2576 3762 4943 6327 6595 1632 Size: 632 196 116 1186 1181 1384 268 1917 Fragments arranged by size: 1917 1384 1186 1181 632 268 196 116 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6683 AflIII A'CryG_T Cuts at: 1203 5779 6756 1203 Size: 4576 977 1327 Fragments arranged by size: 4576 1327 977 AgeI A'CCGG_T Cuts at: 5711 5711 Size: 6880 AhdI GACnn_n'nnGTC Cuts at: 5609 5609 Size: 6880 AleI CACnn'nnGTG Cuts at: 1166 3593 1166 Size: 2427 4453 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6880 AlwNI CAG_nnn'CTG Cuts at: 4623 6195 4623 Size: 1572 5308 ApaI G_GGCC'C Cuts at: 2202 2202 Size: 6880 ApaLI G'TGCA_C Cuts at: 4937 6093 4937 Size: 1156 5724 ApoI r'AATT_y Cuts at: 1493 3338 3565 4189 4415 4567 6676 1493 Size: 1845 227 624 226 152 2109 1697 Fragments arranged by size: 2109 1845 1697 624 227 226 152 AseI AT'TA_AT Cuts at: 160 1032 5434 160 Size: 872 4402 1606 Fragments arranged by size: 4402 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6880 AvaI C'yCGr_G Cuts at: 1870 1943 2427 4205 1870 Size: 73 484 1778 4545 Fragments arranged by size: 4545 1778 484 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2966 2999 2966 Size: 33 6847 BamHI G'GATC_C Cuts at: 4210 4577 4210 Size: 367 6513 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3373 4201 5656 618 Size: 325 217 627 1586 828 1455 1842 Fragments arranged by size: 1842 1586 1455 828 627 325 217 BanII G_rGCy'C Cuts at: 1391 2202 4199 1391 Size: 811 1997 4072 Fragments arranged by size: 4072 1997 811 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3186 928 Size: 971 1287 4622 Fragments arranged by size: 4622 1287 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5071 5105 1903 Size: 34 3134 34 3678 Fragments arranged by size: 3678 3134 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2476 3784 3964 4772 5982 2476 Size: 1308 180 808 1210 3374 Fragments arranged by size: 3374 1308 1210 808 180 BclI T'GATC_A Cuts at: 1548 1548 Size: 6880 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5491 136 Size: 107 122 71 631 605 3819 1525 Fragments arranged by size: 3819 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6875 6875 Size: 6880 BlpI GC'TnA_GC Cuts at: 4297 4297 Size: 6880 Bme1580I G_kGCm'C Cuts at: 1790 2202 4941 6097 1790 Size: 412 2739 1156 2573 Fragments arranged by size: 2739 2573 1156 412 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6880 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2445 2791 4611 Size: 416 53 83 399 24 1028 346 1820 Cuts at: 4611 5569 442 Size: 958 1753 Fragments arranged by size: 1820 1753 1028 958 416 399 346 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6880 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2086 5540 1239 Size: 847 3454 2579 Fragments arranged by size: 3454 2579 847 Bpu10I CC'TnA_GC Cuts at: 3630 3630 Size: 6880 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2055 3246 3472 5005 5870 6168 2055 Size: 1191 226 1533 865 298 2767 Fragments arranged by size: 2767 1533 1191 865 298 226 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3530 5543 882 Size: 617 2031 2013 2219 Fragments arranged by size: 2219 2031 2013 617 BsaAI yAC'GTr Cuts at: 493 1204 4671 493 Size: 711 3467 2702 Fragments arranged by size: 3467 2702 711 BsaBI GATnn'nnATC Cuts at: 3924 6874 3924 Size: 2950 3930 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4687 5069 5704 275 Size: 53 83 186 936 3154 382 635 1451 Fragments arranged by size: 3154 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2558 5313 5711 5985 6132 6543 2558 Size: 2755 398 274 147 411 2895 Fragments arranged by size: 2895 2755 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2849 2879 3453 3483 3632 Size: 30 165 30 1446 30 574 30 149 Cuts at: 3632 3662 1178 Size: 30 4396 Fragments arranged by size: 4396 1446 574 165 149 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3640 3649 1186 Size: 2454 9 4417 Fragments arranged by size: 4417 2454 9 BseYI C'CCAG_C Cuts at: 2761 6083 6579 2761 Size: 3322 496 3062 Fragments arranged by size: 3322 3062 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4040 1163 Size: 573 2304 4003 Fragments arranged by size: 4003 2304 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4330 5091 6119 1540 Size: 275 186 2329 761 1028 2301 Fragments arranged by size: 2329 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2071 4941 5026 6097 2071 Size: 2870 85 1071 2854 Fragments arranged by size: 2870 2854 1071 85 BsmI GAATG_Cn' Cuts at: 2568 4391 4484 2568 Size: 1823 93 4964 Fragments arranged by size: 4964 1823 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2202 4199 4941 5026 6097 1391 Size: 399 281 131 1997 742 85 1071 2174 Fragments arranged by size: 2174 1997 1071 742 399 281 131 85 BspEI T'CCGG_A Cuts at: 6543 6543 Size: 6880 BspHI T'CATG_A Cuts at: 3142 4769 6499 3142 Size: 1627 1730 3523 Fragments arranged by size: 3523 1730 1627 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2910 4235 844 Size: 2066 1325 3489 Fragments arranged by size: 3489 2066 1325 BsrBI CCG'CTC Cuts at: 1394 1823 4767 1394 Size: 429 2944 3507 Fragments arranged by size: 3507 2944 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2537 3942 5375 5549 65 Size: 1183 1289 1405 1433 174 1396 Fragments arranged by size: 1433 1405 1396 1289 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5524 5711 1700 Size: 110 3714 187 2869 Fragments arranged by size: 3714 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3109 96 Size: 3013 3867 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6880 BssSI C'ACGA_G Cuts at: 2157 4940 5952 2157 Size: 2783 1012 3085 Fragments arranged by size: 3085 2783 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6880 BstBI TT'CG_AA Cuts at: 5684 5684 Size: 6880 BstEII G'GTnAC_C Cuts at: 1184 3902 1184 Size: 2718 4162 BstXI CCAn_nnnn'nTGG Cuts at: 3595 3595 Size: 6880 BstYI r'GATC_y Cuts at: 1972 4210 4577 4962 4979 6431 6517 6529 6875 Size: 2238 367 385 17 1452 86 12 346 Cuts at: 6875 1972 Size: 1977 Fragments arranged by size: 2238 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2397 2397 Size: 6880 BtgI C'CryG_G Cuts at: 513 1065 2004 2665 3897 513 Size: 552 939 661 1232 3496 Fragments arranged by size: 3496 1232 939 661 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6141 Fragments arranged by size: 6141 683 56 BtsI GCAGTG_nn' Cuts at: 4396 5190 5210 4396 Size: 794 20 6066 Fragments arranged by size: 6066 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6880 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6845 DraI TTT'AAA Cuts at: 4184 4184 Size: 6880 DraIII CAC_nnn'GTG Cuts at: 2732 2732 Size: 6880 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2749 5887 809 Size: 507 1433 3138 1802 Fragments arranged by size: 3138 1802 1433 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4327 5216 6845 Size: 54 1425 50 275 18 2497 889 1629 Cuts at: 6845 8 Size: 43 Fragments arranged by size: 2497 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4327 1537 Size: 275 2515 4090 Fragments arranged by size: 4090 2515 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2046 2436 2595 3201 4810 1353 Size: 693 390 159 606 1609 3423 Fragments arranged by size: 3423 1609 693 606 390 159 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5467 5841 5987 387 Size: 1064 138 3878 374 146 1280 Fragments arranged by size: 3878 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2086 2264 2460 2576 3762 4943 5540 Size: 393 454 178 196 116 1186 1181 597 Cuts at: 5540 6327 6595 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1186 1181 787 597 454 393 268 196 178 116 EcoO109I rG'GnC_Cy Cuts at: 2198 2199 2585 2742 2198 Size: 1 386 157 6336 Fragments arranged by size: 6336 386 157 1 EcoRI G'AATT_C Cuts at: 4189 4567 4189 Size: 378 6502 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2594 2940 3091 2594 Size: 346 151 6383 Fragments arranged by size: 6383 346 151 FspI TGC'GCA Cuts at: 5386 5386 Size: 6880 HaeII r_GCGC'y Cuts at: 1678 6027 1678 Size: 4349 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2203 2235 2380 2412 4037 Size: 32 135 32 631 32 145 32 1625 Cuts at: 4037 4069 5521 5553 5595 5627 1373 Size: 32 1452 32 42 32 2626 Fragments arranged by size: 2626 1625 1452 631 145 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2035 4224 4470 669 Size: 988 281 97 2189 246 3079 Fragments arranged by size: 3079 2189 988 281 246 97 HindIII A'AGCT_T Cuts at: 748 3051 748 Size: 2303 4577 HpaI GTT'AAC Cuts at: 4470 4470 Size: 6880 KpnI G_GTAC'C Cuts at: 3377 4205 3377 Size: 828 6052 MfeI C'AATT_G Cuts at: 4479 4479 Size: 6880 MluI A'CGCG_T Cuts at: 6756 6756 Size: 6880 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3428 3937 5994 6178 1305 Size: 2123 509 2057 184 2007 Fragments arranged by size: 2123 2057 2007 509 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5058 Fragments arranged by size: 5058 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2617 3432 3593 4091 4838 5197 5356 Size: 648 1451 815 161 498 747 359 159 Cuts at: 5356 518 Size: 2042 Fragments arranged by size: 2042 1451 815 747 648 498 359 161 159 MspA1I CmG'CkG Cuts at: 1606 3740 4296 4973 6121 6366 1606 Size: 2134 556 677 1148 245 2120 Fragments arranged by size: 2134 2120 1148 677 556 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3897 513 Size: 552 939 1893 3496 Fragments arranged by size: 3496 1893 939 552 NdeI CA'TA_TG Cuts at: 387 2809 387 Size: 2422 4458 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6880 NotI GC'GGCC_GC Cuts at: 4327 4327 Size: 6880 NspI r_CATG'y Cuts at: 1132 1229 3064 3867 4238 5783 1132 Size: 97 1835 803 371 1545 2229 Fragments arranged by size: 2229 1835 1545 803 371 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6880 PciI A'CATG_T Cuts at: 5779 5779 Size: 6880 PflMI CCAn_nnn'nTGG Cuts at: 1108 2245 2813 1108 Size: 1137 568 5175 Fragments arranged by size: 5175 1137 568 PmeI GTTT'AAAC Cuts at: 4184 4184 Size: 6880 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4949 4981 6462 6494 4949 Size: 32 1481 32 5335 Fragments arranged by size: 5335 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2742 2742 Size: 6880 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6880 PsiI TTA'TAA Cuts at: 4450 4450 Size: 6880 PspOMI G'GGCC_C Cuts at: 2198 2198 Size: 6880 PstI C_TGCA'G Cuts at: 830 2230 3220 4232 830 Size: 1400 990 1012 3478 Fragments arranged by size: 3478 1400 1012 990 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6880 PvuII CAG'CTG Cuts at: 3740 3740 Size: 6880 SalI G'TCGA_C Cuts at: 1936 4222 1936 Size: 2286 4594 SanDI GG'GwC_CC Cuts at: 2742 2742 Size: 6880 SbfI CC_TGCA'GG Cuts at: 4232 4232 Size: 6880 ScaI AGT'ACT Cuts at: 3886 5128 6840 3886 Size: 1242 1712 3926 Fragments arranged by size: 3926 1712 1242 SfcI C'TryA_G Cuts at: 826 1045 2136 2226 3003 3216 3510 3689 4176 Size: 219 1091 90 777 213 294 179 487 Cuts at: 4176 4228 5363 6044 6235 826 Size: 52 1135 681 191 1471 Fragments arranged by size: 1471 1135 1091 777 681 487 294 219 213 191 179 90 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6880 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6880 SmaI CCC'GGG Cuts at: 1872 4207 1872 Size: 2335 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2070 3261 3451 4984 5885 6147 Size: 197 926 127 1191 190 1533 901 262 Cuts at: 6147 820 Size: 1553 Fragments arranged by size: 1553 1533 1191 926 901 262 197 190 127 SnaBI TAC'GTA Cuts at: 493 4671 493 Size: 4178 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6880 SphI G_CATG'C Cuts at: 1132 4238 1132 Size: 3106 3774 SspI AAT'ATT Cuts at: 5 52 3526 3678 4804 5 Size: 47 3474 152 1126 2081 Fragments arranged by size: 3474 2081 1126 152 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6880 StyI C'CwwG_G Cuts at: 513 1065 2004 3710 3897 4319 513 Size: 552 939 1706 187 422 3074 Fragments arranged by size: 3074 1706 939 552 422 187 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4907 4924 5077 5262 5707 325 Size: 1255 3327 17 153 185 445 1498 Fragments arranged by size: 3327 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3109 3884 5126 Size: 274 80 33 51 1443 1132 775 1242 Cuts at: 5126 6838 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1242 1132 775 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3632 3664 6840 6872 3632 Size: 32 3176 32 3640 Fragments arranged by size: 3640 3176 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6880 XbaI T'CTAG_A Cuts at: 4216 4216 Size: 6880 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6880 XmaI C'CCGG_G Cuts at: 1870 4205 1870 Size: 2335 4545 XmnI GAAnn'nnTTC Cuts at: 2577 5009 2577 Size: 2432 4448 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4688 276 Size: 53 83 186 936 3154 2468 Fragments arranged by size: 3154 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI