Restriction map for
pFN21AE5222
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiWI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII ClaI
CpoI DraI DraII DraIII DrdI
DsaI Eam1105I Eco47III EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI StuI StyI
SwaI TaqI TfiI Tru9I TspRI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AocI 444 CCTGAGG CC^T[ACGT]AGG
AvaI 368 CTCGGG C^[CT]CG[GA]G
AvaII 228 GGACC G^G[AT]CC
BalI 212 TGGCCA TGG^CCA
BbvCI 660 CCTCAGC CCTCAGC(-5/-2)
BsaI 49 GAGACC GGTCTC(1/5)
BsgI 565 CTGCAC GTGCAG(16/14)
BsiEI 710 CGGCCG CG[GA][CT]^CG
BsoBI 368 CTCGGG C^[CT]CG[GA]G
BstYI 183 AGATCC [GA]^GATC[CT]
Bsu36I 444 CCTGAGG CC^T[ACGT]AGG
CfoI 606 GCGC GCG^C
EagI 710 CGGCCG C^GGCCG
EarI 499 CTCTTC CTCTTC(1/4)
EclXI 710 CGGCCG C^GGCCG
Eco52I 710 CGGCCG C^GGCCG
Eco81I 444 CCTGAGG CC^T[ACGT]AGG
FauI 161 CCCGC CCCGC(4/6)
HgaI 269 GACGC GACGC(5/10)
HhaI 606 GCGC GCG^C
HinP1I 606 GCGC G^CGC
HinfI 335 GACTC G^A[ACGT]TC
Ksp632I 499 CTCTTC CTCTTC(1/4)
MaeII 330 ACGT A^CGT
MflI 183 AGATCC [GA]^GATC[CT]
MluNI 212 TGGCCA TGG^CCA
MscI 212 TGGCCA TGG^CCA
MslI 459 CATGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 712 GCCGGC GCC^GGC
NgoMIV 712 GCCGGC G^CCGGC
NlaIV 6 GGAGCC GG[ACGT]^[ACGT]CC
PleI 335 GACTC GAGTC(4/5)
SacI 616 GAGCTC GAGCT^C
SbfI 393 CCTGCAGG CCTGCA^GG
Sse8387I 393 CCTGCAGG CCTGCA^GG
SspI 110 AATATT AAT^ATT
Tsp45I 415 GTCAC ^GT[GC]AC
Tsp509I 139 AATT ^AATT
XhoII 183 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 162 CCGC CCGC(-3/-1)
709 GCGG
AfaI 177 GTAC GT^AC
360 GTAC
AlwI 184 GATCC GGATC(4/5)
630 GATCC
AspHI 383 GAGCAC G[AT]GC[AT]^C
616 GAGCTC
BanII 7 GAGCCC G[GA]GC[CT]^C
616 GAGCTC
BpmI 586 CTGGAG CTGGAG(16/14)
688 CTGGAG
BsaJI 473 CCCTGG C^C[ACGT][ACGT]GG
668 CCCTGG
BsiHKAI 383 GAGCAC G[AT]GC[AT]^C
616 GAGCTC
BsiYI 363 CCAGGCTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
570 CCGGGTTGGGG
BslI 363 CCAGGCTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
570 CCGGGTTGGGG
BsrFI 344 ACCGGC [GA]^CCGG[CT]
712 GCCGGC
BstF5I 612 GGATG GGATG(2/0)
636 GGATG
Cfr10I 344 ACCGGC [GA]^CCGG[CT]
712 GCCGGC
DdeI 445 CTGAG C^T[ACGT]AG
661 CTCAG
EaeI 212 TGGCCA [CT]^GGCC[GA]
710 CGGCCG
FokI 612 GGATG GGATG(9/13)
636 GGATG
MaeIII 167 GTAAC ^GT[ACGT]AC
415 GTCAC
NspI 272 GCATGT [GA]CATG^[CT]
458 ACATGC
RsaI 177 GTAC GT^AC
360 GTAC
SfaNI 43 GATGC GCATC(5/9)
153 GATGC
XcmI 215 CCAATGTGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
464 CCATGATGGCCCTGG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwNI BbsI BbvI BcnI
BmyI BpuAI BseRI BsmAI Bsp1286I
BsrI BssKI BstNI Cac8I Cfr13I
DpnII EcoRII Fnu4HI HaeIII HapII
HpaII HphI ItaI MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII PstI PvuII
Sau3AI Sau96I ScrFI SfcI TseI