(Circular) (Six-base) MAPSORT of: pFN21AE5222.seq Check: 6937 from: 1 to: 5473 pFN21AE5222 5473 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3283 278 Size: 53 83 186 936 1747 2468 Fragments arranged by size: 2468 1747 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2816 1937 Size: 879 4594 Acc65I G'GTAC_C Cuts at: 2794 2794 Size: 5473 AclI AA'CG_TT Cuts at: 1563 3600 3973 1563 Size: 2037 373 3063 Fragments arranged by size: 3063 2037 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2090 3536 4920 5188 1632 Size: 458 1446 1384 268 1917 Fragments arranged by size: 1917 1446 1384 458 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5276 AflIII A'CryG_T Cuts at: 1203 4372 5349 1203 Size: 3169 977 1327 Fragments arranged by size: 3169 1327 977 AgeI A'CCGG_T Cuts at: 4304 4304 Size: 5473 AhdI GACnn_n'nnGTC Cuts at: 4202 4202 Size: 5473 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5473 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5473 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2240 2272 2240 Size: 32 5441 AlwNI CAG_nnn'CTG Cuts at: 2065 2068 2686 3216 4788 2065 Size: 3 618 530 1572 2750 Fragments arranged by size: 2750 1572 618 530 3 ApaLI G'TGCA_C Cuts at: 3530 4686 3530 Size: 1156 4317 ApoI r'AATT_y Cuts at: 1493 2782 3008 3160 5269 1493 Size: 1289 226 152 2109 1697 Fragments arranged by size: 2109 1697 1289 226 152 AseI AT'TA_AT Cuts at: 160 1032 4027 160 Size: 872 2995 1606 Fragments arranged by size: 2995 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 AvaI C'yCGr_G Cuts at: 1870 1943 2373 2798 1870 Size: 73 430 425 4545 Fragments arranged by size: 4545 430 425 73 BamHI G'GATC_C Cuts at: 2803 3170 2803 Size: 367 5106 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2794 4249 618 Size: 325 217 627 1007 1455 1842 Fragments arranged by size: 1842 1455 1007 627 325 217 BanII G_rGCy'C Cuts at: 1391 2016 2625 2792 1391 Size: 625 609 167 4072 Fragments arranged by size: 4072 625 609 167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2078 2320 2732 928 Size: 971 179 242 412 3669 Fragments arranged by size: 3669 971 412 242 179 BbvCI CC'TCA_GC Cuts at: 2666 2666 Size: 5473 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3664 3698 1903 Size: 34 1727 34 3678 Fragments arranged by size: 3678 1727 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3365 4575 3365 Size: 1210 4263 BclI T'GATC_A Cuts at: 1548 1548 Size: 5473 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4084 136 Size: 107 122 71 631 605 2412 1525 Fragments arranged by size: 2412 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5468 5468 Size: 5473 BlpI GC'TnA_GC Cuts at: 2890 2890 Size: 5473 Bme1580I G_kGCm'C Cuts at: 1790 3534 4690 1790 Size: 1744 1156 2573 Fragments arranged by size: 2573 1744 1156 BmgBI CAC'GTC Cuts at: 1318 2336 1318 Size: 1018 4455 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2010 3204 4162 Size: 416 53 83 399 24 593 1194 958 Cuts at: 4162 442 Size: 1753 Fragments arranged by size: 1753 1194 958 593 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5473 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2612 2714 4133 1239 Size: 1373 102 1419 2579 Fragments arranged by size: 2579 1419 1373 102 Bpu10I CC'TnA_GC Cuts at: 2666 2666 Size: 5473 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3598 4463 4761 3598 Size: 865 298 4310 Fragments arranged by size: 4310 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2048 4136 882 Size: 617 549 2088 2219 Fragments arranged by size: 2219 2088 617 549 BsaAI yAC'GTr Cuts at: 493 1204 3264 493 Size: 711 2060 2702 Fragments arranged by size: 2702 2060 711 BsaBI GATnn'nnATC Cuts at: 5467 5467 Size: 5473 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3280 3662 4297 275 Size: 53 83 186 936 1747 382 635 1451 Fragments arranged by size: 1747 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3906 4304 4578 4725 5136 3906 Size: 398 274 147 411 4243 Fragments arranged by size: 4243 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2125 2155 2325 2355 1178 Size: 30 165 30 722 30 170 30 4296 Fragments arranged by size: 4296 722 170 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2146 2333 2555 2558 1186 Size: 853 107 187 222 3 4101 Fragments arranged by size: 4101 853 222 187 107 3 BseYI C'CCAG_C Cuts at: 2058 2406 2517 2679 4676 5172 2058 Size: 348 111 162 1997 496 2359 Fragments arranged by size: 2359 1997 496 348 162 111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2555 1163 Size: 573 819 4081 Fragments arranged by size: 4081 819 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2718 2923 3684 4712 1540 Size: 275 186 717 205 761 1028 2301 Fragments arranged by size: 2301 1028 761 717 275 205 186 BsiHKAI G_wGCw'C Cuts at: 2392 2625 3534 3619 4690 2392 Size: 233 909 85 1071 3175 Fragments arranged by size: 3175 1071 909 233 85 BsmI GAATG_Cn' Cuts at: 2984 3077 2984 Size: 93 5380 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2392 2625 2792 3534 3619 4690 Size: 399 226 376 233 167 742 85 1071 Cuts at: 4690 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 376 233 226 167 85 BspEI T'CCGG_A Cuts at: 5136 5136 Size: 5473 BspHI T'CATG_A Cuts at: 3362 5092 3362 Size: 1730 3743 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2828 844 Size: 1984 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3360 1394 Size: 429 1537 3507 Fragments arranged by size: 3507 1537 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3968 4142 65 Size: 1183 2720 174 1396 Fragments arranged by size: 2720 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2349 2717 4117 4304 1700 Size: 110 539 368 1400 187 2869 Fragments arranged by size: 2869 1400 539 368 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5473 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5473 BssSI C'ACGA_G Cuts at: 3533 4545 3533 Size: 1012 4461 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5473 BstBI TT'CG_AA Cuts at: 4277 4277 Size: 5473 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5473 BstYI r'GATC_y Cuts at: 1972 2188 2803 3170 3555 3572 5024 5110 5122 Size: 216 615 367 385 17 1452 86 12 Cuts at: 5122 5468 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 615 385 367 346 216 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2450 2450 Size: 5473 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3982 Fragments arranged by size: 3982 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4734 Fragments arranged by size: 4734 683 56 BtsI GCAGTG_nn' Cuts at: 2989 3783 3803 2989 Size: 794 20 4659 Fragments arranged by size: 4659 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5473 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5438 DraI TTT'AAA Cuts at: 2777 2777 Size: 5473 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4480 809 Size: 507 3164 1802 Fragments arranged by size: 3164 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2217 2715 2920 Size: 54 1425 50 275 18 387 498 205 Cuts at: 2920 3809 5438 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 498 387 275 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2715 2920 1537 Size: 275 903 205 4090 Fragments arranged by size: 4090 903 275 205 EarI CTCTTCn'nnn_ Cuts at: 1353 2510 3403 1353 Size: 1157 893 3423 Fragments arranged by size: 3423 1157 893 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4060 4434 4580 387 Size: 1064 138 2471 374 146 1280 Fragments arranged by size: 2471 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2623 2623 Size: 5473 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2612 2714 3536 4133 4920 5188 Size: 393 458 522 102 822 597 787 268 Cuts at: 5188 1239 Size: 1524 Fragments arranged by size: 1524 822 787 597 522 458 393 268 102 EcoRI G'AATT_C Cuts at: 2782 3160 2782 Size: 378 5095 FspI TGC'GCA Cuts at: 3979 3979 Size: 5473 HaeII r_GCGC'y Cuts at: 1678 4620 1678 Size: 2942 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2325 2357 4114 4146 4188 Size: 32 135 32 753 32 1757 32 42 Cuts at: 4188 4220 1373 Size: 32 2626 Fragments arranged by size: 2626 1757 753 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2817 3063 669 Size: 988 281 879 246 3079 Fragments arranged by size: 3079 988 879 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5473 HpaI GTT'AAC Cuts at: 3063 3063 Size: 5473 KpnI G_GTAC'C Cuts at: 2798 2798 Size: 5473 MfeI C'AATT_G Cuts at: 3072 3072 Size: 5473 MluI A'CGCG_T Cuts at: 5349 5349 Size: 5473 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2356 2793 4587 4771 1305 Size: 1051 437 1794 184 2007 Fragments arranged by size: 2007 1794 1051 437 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2219 10 Size: 54 1425 343 387 3264 Fragments arranged by size: 3264 1425 387 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2468 3431 3790 3949 518 Size: 648 1302 963 359 159 2042 Fragments arranged by size: 2042 1302 963 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2065 2169 2383 2569 2590 2683 2738 2889 Size: 459 104 214 186 21 93 55 151 Cuts at: 2889 3566 4714 4959 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 459 245 214 186 151 104 93 55 21 NaeI GCC'GGC Cuts at: 2719 2719 Size: 5473 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3982 Fragments arranged by size: 3982 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5473 NgoMIV G'CCGG_C Cuts at: 2717 2717 Size: 5473 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5473 NotI GC'GGCC_GC Cuts at: 2920 2920 Size: 5473 NspI r_CATG'y Cuts at: 1132 1229 2281 2467 2831 4376 1132 Size: 97 1052 186 364 1545 2229 Fragments arranged by size: 2229 1545 1052 364 186 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5473 PciI A'CATG_T Cuts at: 4372 4372 Size: 5473 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5473 PfoI T'CCnGG_A Cuts at: 2637 2637 Size: 5473 PmeI GTTT'AAAC Cuts at: 2777 2777 Size: 5473 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3542 3574 5055 5087 3542 Size: 32 1481 32 3928 Fragments arranged by size: 3928 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5473 PsiI TTA'TAA Cuts at: 3043 3043 Size: 5473 PstI C_TGCA'G Cuts at: 830 2247 2358 2403 2568 2825 830 Size: 1417 111 45 165 257 3478 Fragments arranged by size: 3478 1417 257 165 111 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5473 PvuII CAG'CTG Cuts at: 2065 2383 2569 2590 2683 2738 2065 Size: 318 186 21 93 55 4800 Fragments arranged by size: 4800 318 186 93 55 21 SacI G_AGCT'C Cuts at: 2625 2625 Size: 5473 SalI G'TCGA_C Cuts at: 1936 2815 1936 Size: 879 4594 SbfI CC_TGCA'GG Cuts at: 2403 2825 2403 Size: 422 5051 ScaI AGT'ACT Cuts at: 3721 5433 3721 Size: 1712 3761 SfcI C'TryA_G Cuts at: 826 1045 2137 2243 2354 2399 2564 2755 2821 Size: 219 1092 106 111 45 165 191 66 Cuts at: 2821 3956 4637 4828 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1092 681 219 191 191 165 111 106 66 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5473 SmaI CCC'GGG Cuts at: 1872 2800 1872 Size: 928 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3577 4478 4740 820 Size: 197 926 1634 901 262 1553 Fragments arranged by size: 1634 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3264 493 Size: 2771 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5473 SphI G_CATG'C Cuts at: 1132 2831 1132 Size: 1699 3774 SspI AAT'ATT Cuts at: 5 52 2117 3397 5 Size: 47 2065 1280 2081 Fragments arranged by size: 2081 2065 1280 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5473 StyI C'CwwG_G Cuts at: 513 1065 2004 2912 513 Size: 552 939 908 3074 Fragments arranged by size: 3074 939 908 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3500 3517 3670 3855 4300 325 Size: 1255 1920 17 153 185 445 1498 Fragments arranged by size: 1920 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 3719 5431 Size: 274 80 33 51 1443 204 1538 1712 Cuts at: 5431 96 Size: 138 Fragments arranged by size: 1712 1538 1443 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5433 5465 5433 Size: 32 5441 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5473 XbaI T'CTAG_A Cuts at: 2809 2809 Size: 5473 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2227 2476 2227 Size: 249 5224 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5473 XmaI C'CCGG_G Cuts at: 1870 2798 1870 Size: 928 4545 XmnI GAAnn'nnTTC Cuts at: 3602 3602 Size: 5473 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3281 276 Size: 53 83 186 936 1747 2468 Fragments arranged by size: 2468 1747 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI