(Linear) (Six-base) MAPSORT of: KIEE5222.seq Check: 2818 from: 1 to: 768 KIEE5222.orf With 182 enzymes: SgfI * January 29, 2014 11:51 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 768 Size: 85 683 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 235 267 768 Size: 235 32 501 Fragments arranged by size: 501 235 32 AlwNI CAG_nnn'CTG Cuts at: 0 60 63 681 768 Size: 60 3 618 87 Fragments arranged by size: 618 87 60 3 AvaI C'yCGr_G Cuts at: 0 368 768 Size: 368 400 BanII G_rGCy'C Cuts at: 0 11 620 768 Size: 11 609 148 Fragments arranged by size: 609 148 11 BbsI GAAGACnn'nnnn_ Cuts at: 0 73 315 727 768 Size: 73 242 412 41 Fragments arranged by size: 412 242 73 41 BbvCI CC'TCA_GC Cuts at: 0 661 768 Size: 661 107 BmgBI CAC'GTC Cuts at: 0 331 768 Size: 331 437 BmrI ACTGGGnnnn_n' Cuts at: 0 5 768 Size: 5 763 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 607 709 768 Size: 607 102 59 Fragments arranged by size: 607 102 59 Bpu10I CC'TnA_GC Cuts at: 0 661 768 Size: 661 107 BsaI GGTCTCn'nnnn_ Cuts at: 0 43 768 Size: 43 725 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 120 150 320 350 768 Size: 120 30 170 30 418 Fragments arranged by size: 418 170 120 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 34 141 328 550 553 768 Size: 34 107 187 222 3 215 Fragments arranged by size: 222 215 187 107 34 3 BseYI C'CCAG_C Cuts at: 0 53 401 512 674 768 Size: 53 348 111 162 94 Fragments arranged by size: 348 162 111 94 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 550 768 Size: 550 218 BsiEI CG_ry'CG Cuts at: 0 713 768 Size: 713 55 BsiHKAI G_wGCw'C Cuts at: 0 387 620 768 Size: 387 233 148 Fragments arranged by size: 387 233 148 Bsp1286I G_dGCh'C Cuts at: 0 11 387 620 768 Size: 11 376 233 148 Fragments arranged by size: 376 233 148 11 BsrFI r'CCGG_y Cuts at: 0 344 712 768 Size: 344 368 56 Fragments arranged by size: 368 344 56 BstYI r'GATC_y Cuts at: 0 183 768 Size: 183 585 Bsu36I CC'TnA_GG Cuts at: 0 445 768 Size: 445 323 EaeI y'GGCC_r Cuts at: 0 212 710 768 Size: 212 498 58 Fragments arranged by size: 498 212 58 EagI C'GGCC_G Cuts at: 0 710 768 Size: 710 58 EarI CTCTTCn'nnn_ Cuts at: 0 505 768 Size: 505 263 EcoICRI GAG'CTC Cuts at: 0 618 768 Size: 618 150 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 607 709 768 Size: 85 522 102 59 Fragments arranged by size: 522 102 85 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 320 352 768 Size: 320 32 416 Fragments arranged by size: 416 320 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 351 768 Size: 351 417 MscI TGG'CCA Cuts at: 0 214 768 Size: 214 554 MslI CAynn'nnrTG Cuts at: 0 463 768 Size: 463 305 MspA1I CmG'CkG Cuts at: 0 60 164 378 564 585 678 733 768 Size: 60 104 214 186 21 93 55 35 Fragments arranged by size: 214 186 104 93 60 55 35 21 NaeI GCC'GGC Cuts at: 0 714 768 Size: 714 54 NgoMIV G'CCGG_C Cuts at: 0 712 768 Size: 712 56 NspI r_CATG'y Cuts at: 0 276 462 768 Size: 276 186 306 Fragments arranged by size: 306 276 186 PfoI T'CCnGG_A Cuts at: 0 632 768 Size: 632 136 PstI C_TGCA'G Cuts at: 0 242 353 398 563 768 Size: 242 111 45 165 205 Fragments arranged by size: 242 205 165 111 45 PvuII CAG'CTG Cuts at: 0 60 378 564 585 678 733 768 Size: 60 318 186 21 93 55 35 Fragments arranged by size: 318 186 93 60 55 35 21 SacI G_AGCT'C Cuts at: 0 620 768 Size: 620 148 SbfI CC_TGCA'GG Cuts at: 0 398 768 Size: 398 370 SfcI C'TryA_G Cuts at: 0 132 238 349 394 559 750 768 Size: 132 106 111 45 165 191 18 Fragments arranged by size: 191 165 132 111 106 45 18 SspI AAT'ATT Cuts at: 0 112 768 Size: 112 656 TatI w'GTAC_w Cuts at: 0 176 768 Size: 176 592 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 222 471 768 Size: 222 249 297 Fragments arranged by size: 297 249 222 Enzymes that do cut: AcuI AloI AlwNI AvaI BanII BbsI BbvCI BmgBI BmrI BpmI Bpu10I BsaI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrFI BstYI Bsu36I EaeI EagI EarI EcoICRI Eco57MI Hin4I MmeI MscI MslI MspA1I NaeI NgoMIV NspI PfoI PstI PvuII SacI SbfI SfcI SspI TatI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI