(Circular) (Six-base) MAPSORT of: pFN21AE1083.seq Check: 5665 from: 1 to: 5815 pFN21AE1083 5815 bp With 182 enzymes: SgfI * September 25, 2009 17:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3625 278 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3158 1937 Size: 1221 4594 Acc65I G'GTAC_C Cuts at: 3136 3136 Size: 5815 AclI AA'CG_TT Cuts at: 1563 3942 4315 1563 Size: 2379 373 3063 Fragments arranged by size: 3063 2379 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2820 3035 3878 5262 5530 1632 Size: 1188 215 843 1384 268 1917 Fragments arranged by size: 1917 1384 1188 843 268 215 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5618 AflIII A'CryG_T Cuts at: 1203 4714 5691 1203 Size: 3511 977 1327 Fragments arranged by size: 3511 1327 977 AgeI A'CCGG_T Cuts at: 4646 4646 Size: 5815 AhdI GACnn_n'nnGTC Cuts at: 4544 4544 Size: 5815 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5815 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5815 AlwNI CAG_nnn'CTG Cuts at: 3558 5130 3558 Size: 1572 4243 ApaLI G'TGCA_C Cuts at: 3872 5028 3872 Size: 1156 4659 ApoI r'AATT_y Cuts at: 1493 2404 3124 3350 3502 5611 1493 Size: 911 720 226 152 2109 1697 Fragments arranged by size: 2109 1697 911 720 226 152 AseI AT'TA_AT Cuts at: 160 1032 2429 4369 160 Size: 872 1397 1940 1606 Fragments arranged by size: 1940 1606 1397 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 AvaI C'yCGr_G Cuts at: 1870 1943 3140 1870 Size: 73 1197 4545 Fragments arranged by size: 4545 1197 73 BamHI G'GATC_C Cuts at: 3145 3512 3145 Size: 367 5448 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3136 4591 618 Size: 325 217 627 1349 1455 1842 Fragments arranged by size: 1842 1455 1349 627 325 217 BanII G_rGCy'C Cuts at: 1391 2701 3134 1391 Size: 1310 433 4072 Fragments arranged by size: 4072 1310 433 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3048 928 Size: 971 1149 3695 Fragments arranged by size: 3695 1149 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4006 4040 1903 Size: 34 2069 34 3678 Fragments arranged by size: 3678 2069 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3707 4917 3707 Size: 1210 4605 BclI T'GATC_A Cuts at: 1548 1548 Size: 5815 BfrBI ATG'CAT Cuts at: 2563 2563 Size: 5815 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4426 136 Size: 107 122 71 631 605 2754 1525 Fragments arranged by size: 2754 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2746 5810 2746 Size: 3064 2751 BlpI GC'TnA_GC Cuts at: 3232 3232 Size: 5815 Bme1580I G_kGCm'C Cuts at: 1790 3876 5032 1790 Size: 2086 1156 2573 Fragments arranged by size: 2573 2086 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5815 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3546 4504 442 Size: 416 53 83 399 24 2129 958 1753 Fragments arranged by size: 2129 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5815 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4475 1239 Size: 3236 2579 Bpu10I CC'TnA_GC Cuts at: 2309 2659 2309 Size: 350 5465 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2784 3940 4805 5103 2784 Size: 1156 865 298 3496 Fragments arranged by size: 3496 1156 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4478 882 Size: 617 2979 2219 Fragments arranged by size: 2979 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3606 493 Size: 711 2402 2702 Fragments arranged by size: 2702 2402 711 BsaBI GATnn'nnATC Cuts at: 2442 5809 2442 Size: 3367 2448 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3622 4004 4639 275 Size: 53 83 186 936 2089 382 635 1451 Fragments arranged by size: 2089 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4248 4646 4920 5067 5478 4248 Size: 398 274 147 411 4585 Fragments arranged by size: 4585 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5590 Fragments arranged by size: 5590 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 2147 2172 2696 1186 Size: 958 3 25 524 4305 Fragments arranged by size: 4305 958 524 25 3 BseYI C'CCAG_C Cuts at: 5018 5514 5018 Size: 496 5319 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5242 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3265 4026 5054 1540 Size: 275 186 1264 761 1028 2301 Fragments arranged by size: 2301 1264 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2050 3007 3876 3961 5032 2050 Size: 957 869 85 1071 2833 Fragments arranged by size: 2833 1071 957 869 85 BsmI GAATG_Cn' Cuts at: 3326 3419 3326 Size: 93 5722 BsmBI CGTCTCn'nnnn_ Cuts at: 2848 2848 Size: 5815 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2701 3007 3134 3876 3961 5032 Size: 399 260 651 306 127 742 85 1071 Cuts at: 5032 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 651 399 306 260 127 85 BspEI T'CCGG_A Cuts at: 5478 5478 Size: 5815 BspHI T'CATG_A Cuts at: 3704 5434 3704 Size: 1730 4085 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3170 844 Size: 2326 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3702 1394 Size: 429 1879 3507 Fragments arranged by size: 3507 1879 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2053 2254 4310 4484 65 Size: 1183 805 201 2056 174 1396 Fragments arranged by size: 2056 1396 1183 805 201 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4459 4646 1700 Size: 110 2649 187 2869 Fragments arranged by size: 2869 2649 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5815 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5815 BssSI C'ACGA_G Cuts at: 2021 3875 4887 2021 Size: 1854 1012 2949 Fragments arranged by size: 2949 1854 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5815 BstBI TT'CG_AA Cuts at: 4619 4619 Size: 5815 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5815 BstYI r'GATC_y Cuts at: 1972 2746 3145 3512 3897 3914 5366 5452 5464 Size: 774 399 367 385 17 1452 86 12 Cuts at: 5464 5810 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 774 399 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2094 513 Size: 552 939 90 4234 Fragments arranged by size: 4234 939 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5076 Fragments arranged by size: 5076 683 56 BtsI GCAGTG_nn' Cuts at: 3331 4125 4145 3331 Size: 794 20 5001 Fragments arranged by size: 5001 794 20 ClaI AT'CG_AT Cuts at: 1554 2316 1554 Size: 762 5053 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5780 DraI TTT'AAA Cuts at: 3119 3119 Size: 5815 DraIII CAC_nnn'GTG Cuts at: 2045 2045 Size: 5815 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4822 809 Size: 507 3506 1802 Fragments arranged by size: 3506 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3262 4151 5780 Size: 54 1425 50 275 18 1432 889 1629 Cuts at: 5780 8 Size: 43 Fragments arranged by size: 1629 1432 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3262 1537 Size: 275 1450 4090 Fragments arranged by size: 4090 1450 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2835 2846 3745 1353 Size: 1482 11 899 3423 Fragments arranged by size: 3423 1482 899 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4402 4776 4922 387 Size: 1064 138 2813 374 146 1280 Fragments arranged by size: 2813 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2820 3035 3878 4475 5262 5530 1239 Size: 393 1188 215 843 597 787 268 1524 Fragments arranged by size: 1524 1188 843 787 597 393 268 215 EcoO109I rG'GnC_Cy Cuts at: 2950 2950 Size: 5815 EcoRI G'AATT_C Cuts at: 2404 3124 3502 2404 Size: 720 378 4717 Fragments arranged by size: 4717 720 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2057 3065 2057 Size: 1008 4807 FspI TGC'GCA Cuts at: 4321 4321 Size: 5815 HaeII r_GCGC'y Cuts at: 1678 4962 1678 Size: 3284 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2956 2988 4456 4488 4530 Size: 32 135 32 1384 32 1468 32 42 Cuts at: 4530 4562 1373 Size: 32 2626 Fragments arranged by size: 2626 1468 1384 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3159 3405 669 Size: 988 281 1221 246 3079 Fragments arranged by size: 3079 1221 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5815 HpaI GTT'AAC Cuts at: 3405 3405 Size: 5815 KpnI G_GTAC'C Cuts at: 3140 3140 Size: 5815 MfeI C'AATT_G Cuts at: 2109 3414 2109 Size: 1305 4510 MluI A'CGCG_T Cuts at: 5691 5691 Size: 5815 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2578 4929 5113 1305 Size: 1273 2351 184 2007 Fragments arranged by size: 2351 2007 1273 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3993 Fragments arranged by size: 3993 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2341 2974 2976 3773 4132 4291 518 Size: 648 1175 633 2 797 359 159 2042 Fragments arranged by size: 2042 1175 797 648 633 359 159 2 MspA1I CmG'CkG Cuts at: 1606 2122 3059 3231 3908 5056 5301 1606 Size: 516 937 172 677 1148 245 2120 Fragments arranged by size: 2120 1148 937 677 516 245 172 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4324 Fragments arranged by size: 4324 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5815 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5815 NotI GC'GGCC_GC Cuts at: 3262 3262 Size: 5815 NsiI A_TGCA'T Cuts at: 2565 2565 Size: 5815 NspI r_CATG'y Cuts at: 1132 1229 3049 3173 4718 1132 Size: 97 1820 124 1545 2229 Fragments arranged by size: 2229 1820 1545 124 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5815 PciI A'CATG_T Cuts at: 4714 4714 Size: 5815 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5815 PmeI GTTT'AAAC Cuts at: 3119 3119 Size: 5815 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3884 3916 5397 5429 3884 Size: 32 1481 32 4270 Fragments arranged by size: 4270 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2950 2950 Size: 5815 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5815 PsiI TTA'TAA Cuts at: 2243 2352 3385 2243 Size: 109 1033 4673 Fragments arranged by size: 4673 1033 109 PstI C_TGCA'G Cuts at: 830 2541 3167 830 Size: 1711 626 3478 Fragments arranged by size: 3478 1711 626 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5815 PvuII CAG'CTG Cuts at: 2122 3059 2122 Size: 937 4878 SalI G'TCGA_C Cuts at: 1936 3157 1936 Size: 1221 4594 SapI GCTCTTCn'nnn_ Cuts at: 2835 2835 Size: 5815 SbfI CC_TGCA'GG Cuts at: 3167 3167 Size: 5815 ScaI AGT'ACT Cuts at: 4063 5775 4063 Size: 1712 4103 SfcI C'TryA_G Cuts at: 826 1045 2537 3163 4298 4979 5170 826 Size: 219 1492 626 1135 681 191 1471 Fragments arranged by size: 1492 1471 1135 681 626 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5815 SmaI CCC'GGG Cuts at: 1872 3142 1872 Size: 1270 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2799 3919 4820 5082 820 Size: 197 926 856 1120 901 262 1553 Fragments arranged by size: 1553 1120 926 901 856 262 197 SnaBI TAC'GTA Cuts at: 493 3606 493 Size: 3113 2702 SpeI A'CTAG_T Cuts at: 152 2165 152 Size: 2013 3802 SphI G_CATG'C Cuts at: 1132 3173 1132 Size: 2041 3774 SspI AAT'ATT Cuts at: 5 52 2084 3739 5 Size: 47 2032 1655 2081 Fragments arranged by size: 2081 2032 1655 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5815 StyI C'CwwG_G Cuts at: 513 1065 2004 3254 513 Size: 552 939 1250 3074 Fragments arranged by size: 3074 1250 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3842 3859 4012 4197 4642 325 Size: 1255 2262 17 153 185 445 1498 Fragments arranged by size: 2262 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4061 5773 96 Size: 274 80 33 51 1443 2084 1712 138 Fragments arranged by size: 2084 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5775 5807 5775 Size: 32 5783 Tth111I GACn'n_nGTC Cuts at: 1316 3067 1316 Size: 1751 4064 XbaI T'CTAG_A Cuts at: 3151 3151 Size: 5815 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5815 XmaI C'CCGG_G Cuts at: 1870 3140 1870 Size: 1270 4545 XmnI GAAnn'nnTTC Cuts at: 2844 3944 2844 Size: 1100 4715 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3623 276 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI