(Circular) (Six-base) MAPSORT of: pFN21AB6595.seq Check: 3911 from: 1 to: 5845 pFN21AB6595 5845 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3655 278 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3188 1937 Size: 1251 4594 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3972 4345 1563 Size: 2409 373 3063 Fragments arranged by size: 3063 2409 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2064 3908 5292 5560 1632 Size: 432 1844 1384 268 1917 Fragments arranged by size: 1917 1844 1384 432 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5648 AflIII A'CryG_T Cuts at: 1203 4744 5721 1203 Size: 3541 977 1327 Fragments arranged by size: 3541 1327 977 AgeI A'CCGG_T Cuts at: 3080 4676 3080 Size: 1596 4249 AhdI GACnn_n'nnGTC Cuts at: 4574 4574 Size: 5845 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5845 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5845 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2343 2375 2343 Size: 32 5813 AlwNI CAG_nnn'CTG Cuts at: 3588 5160 3588 Size: 1572 4273 ApaLI G'TGCA_C Cuts at: 3902 5058 3902 Size: 1156 4689 ApoI r'AATT_y Cuts at: 1493 2406 2964 3128 3154 3380 3532 5641 1493 Size: 913 558 164 26 226 152 2109 1697 Fragments arranged by size: 2109 1697 913 558 226 164 152 26 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 2745 3170 1870 Size: 73 802 425 4545 Fragments arranged by size: 4545 802 425 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2128 2161 2128 Size: 33 5812 BamHI G'GATC_C Cuts at: 2128 3175 3542 2128 Size: 1047 367 4431 Fragments arranged by size: 4431 1047 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3166 4621 618 Size: 325 217 627 1379 1455 1842 Fragments arranged by size: 1842 1455 1379 627 325 217 BanII G_rGCy'C Cuts at: 1391 3164 1391 Size: 1773 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2255 3012 928 Size: 971 356 757 3761 Fragments arranged by size: 3761 971 757 356 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2898 2932 4036 4070 1903 Size: 34 961 34 1104 34 3678 Fragments arranged by size: 3678 1104 961 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2129 3737 4947 2129 Size: 1608 1210 3027 Fragments arranged by size: 3027 1608 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5845 BfrBI ATG'CAT Cuts at: 2546 2546 Size: 5845 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4456 136 Size: 107 122 71 631 605 2784 1525 Fragments arranged by size: 2784 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5840 5840 Size: 5845 BlpI GC'TnA_GC Cuts at: 3262 3262 Size: 5845 Bme1580I G_kGCm'C Cuts at: 1790 3906 5062 1790 Size: 2116 1156 2573 Fragments arranged by size: 2573 2116 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3576 4534 442 Size: 416 53 83 399 24 2159 958 1753 Fragments arranged by size: 2159 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4505 1239 Size: 3266 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3970 4835 5133 3970 Size: 865 298 4682 Fragments arranged by size: 4682 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4508 882 Size: 617 3009 2219 Fragments arranged by size: 3009 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3636 493 Size: 711 2432 2702 Fragments arranged by size: 2702 2432 711 BsaBI GATnn'nnATC Cuts at: 5839 5839 Size: 5845 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3652 4034 4669 275 Size: 53 83 186 936 2119 382 635 1451 Fragments arranged by size: 2119 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3080 4278 4676 4950 5097 5508 3080 Size: 1198 398 274 147 411 3417 Fragments arranged by size: 3417 1198 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5620 Fragments arranged by size: 5620 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2097 1186 Size: 911 4934 BseYI C'CCAG_C Cuts at: 2994 5048 5544 2994 Size: 2054 496 3295 Fragments arranged by size: 3295 2054 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3295 4056 5084 1540 Size: 275 186 1294 761 1028 2301 Fragments arranged by size: 2301 1294 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3906 3991 5062 3906 Size: 85 1071 4689 Fragments arranged by size: 4689 1071 85 BsmI GAATG_Cn' Cuts at: 2201 3356 3449 2201 Size: 1155 93 4597 Fragments arranged by size: 4597 1155 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3164 3906 3991 5062 1391 Size: 399 1374 742 85 1071 2174 Fragments arranged by size: 2174 1374 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5508 5508 Size: 5845 BspHI T'CATG_A Cuts at: 3734 5464 3734 Size: 1730 4115 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3200 844 Size: 2356 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3732 1394 Size: 429 1909 3507 Fragments arranged by size: 3507 1909 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2165 2345 4340 4514 65 Size: 1183 917 180 1995 174 1396 Fragments arranged by size: 1995 1396 1183 917 180 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3080 4489 4676 1700 Size: 110 1270 1409 187 2869 Fragments arranged by size: 2869 1409 1270 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5845 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5845 BssSI C'ACGA_G Cuts at: 3905 4917 3905 Size: 1012 4833 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5845 BstBI TT'CG_AA Cuts at: 4649 4649 Size: 5845 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5845 BstYI r'GATC_y Cuts at: 1972 2128 2675 3175 3542 3927 3944 5396 5482 Size: 156 547 500 367 385 17 1452 86 Cuts at: 5482 5494 5840 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 547 500 385 367 346 156 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2109 2109 Size: 5845 BtgI C'CryG_G Cuts at: 513 1065 2004 2333 513 Size: 552 939 329 4025 Fragments arranged by size: 4025 939 552 329 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5106 Fragments arranged by size: 5106 683 56 BtsI GCAGTG_nn' Cuts at: 3361 4155 4175 3361 Size: 794 20 5031 Fragments arranged by size: 5031 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5810 DraI TTT'AAA Cuts at: 3149 3149 Size: 5845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4852 809 Size: 507 3536 1802 Fragments arranged by size: 3536 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3292 4181 5810 Size: 54 1425 50 275 18 1462 889 1629 Cuts at: 5810 8 Size: 43 Fragments arranged by size: 1629 1462 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3292 1537 Size: 275 1480 4090 Fragments arranged by size: 4090 1480 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3775 1353 Size: 2422 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4432 4806 4952 387 Size: 1064 138 2843 374 146 1280 Fragments arranged by size: 2843 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2064 3908 4505 5292 5560 1239 Size: 393 432 1844 597 787 268 1524 Fragments arranged by size: 1844 1524 787 597 432 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2484 2484 Size: 5845 EcoO109I rG'GnC_Cy Cuts at: 2792 2792 Size: 5845 EcoRI G'AATT_C Cuts at: 2406 3154 3532 2406 Size: 748 378 4719 Fragments arranged by size: 4719 748 378 EcoRV GAT'ATC Cuts at: 2394 2394 Size: 5845 FspI TGC'GCA Cuts at: 4351 4351 Size: 5845 HaeII r_GCGC'y Cuts at: 1678 4992 1678 Size: 3314 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2113 2145 4486 4518 4560 Size: 32 135 32 541 32 2341 32 42 Cuts at: 4560 4592 1373 Size: 32 2626 Fragments arranged by size: 2626 2341 541 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2101 3189 3435 669 Size: 988 281 163 1088 246 3079 Fragments arranged by size: 3079 1088 988 281 246 163 HindIII A'AGCT_T Cuts at: 748 748 Size: 5845 HpaI GTT'AAC Cuts at: 2101 3435 2101 Size: 1334 4511 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 2449 3444 2449 Size: 995 4850 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4959 5143 1305 Size: 3654 184 2007 Fragments arranged by size: 3654 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4023 Fragments arranged by size: 4023 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2338 2551 3803 4162 4321 518 Size: 648 1172 213 1252 359 159 2042 Fragments arranged by size: 2042 1252 1172 648 359 213 159 MspA1I CmG'CkG Cuts at: 1606 3261 3938 5086 5331 1606 Size: 1655 677 1148 245 2120 Fragments arranged by size: 2120 1655 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2333 513 Size: 552 939 329 4025 Fragments arranged by size: 4025 939 552 329 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5845 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NsiI A_TGCA'T Cuts at: 2548 2548 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 3203 4748 1132 Size: 97 1974 1545 2229 Fragments arranged by size: 2229 1974 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5845 PciI A'CATG_T Cuts at: 4744 4744 Size: 5845 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5845 PfoI T'CCnGG_A Cuts at: 2131 2131 Size: 5845 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3914 3946 5427 5459 3914 Size: 32 1481 32 4300 Fragments arranged by size: 4300 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2792 2792 Size: 5845 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5845 PsiI TTA'TAA Cuts at: 3415 3415 Size: 5845 PstI C_TGCA'G Cuts at: 830 3197 830 Size: 2367 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SbfI CC_TGCA'GG Cuts at: 3197 3197 Size: 5845 ScaI AGT'ACT Cuts at: 4093 5805 4093 Size: 1712 4133 SfcI C'TryA_G Cuts at: 826 1045 2862 3193 4328 5009 5200 826 Size: 219 1817 331 1135 681 191 1471 Fragments arranged by size: 1817 1471 1135 681 331 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 3172 1872 Size: 1300 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3949 4850 5112 820 Size: 197 926 2006 901 262 1553 Fragments arranged by size: 2006 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3636 493 Size: 3143 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 3203 1132 Size: 2071 3774 SspI AAT'ATT Cuts at: 5 52 2459 2944 3063 3769 5 Size: 47 2407 485 119 706 2081 Fragments arranged by size: 2407 2081 706 485 119 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5845 StyI C'CwwG_G Cuts at: 513 1065 2004 2333 2850 3284 513 Size: 552 939 329 517 434 3074 Fragments arranged by size: 3074 939 552 517 434 329 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3872 3889 4042 4227 4672 325 Size: 1255 2292 17 153 185 445 1498 Fragments arranged by size: 2292 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2069 2491 4091 Size: 274 80 33 51 1443 92 422 1600 Cuts at: 4091 5803 96 Size: 1712 138 Fragments arranged by size: 1712 1600 1443 422 274 138 92 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5805 5837 5805 Size: 32 5813 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5845 XbaI T'CTAG_A Cuts at: 3181 3181 Size: 5845 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5845 XmaI C'CCGG_G Cuts at: 1870 3170 1870 Size: 1300 4545 XmnI GAAnn'nnTTC Cuts at: 3974 3974 Size: 5845 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3653 276 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI