(Circular) (Six-base) MAPSORT of: pFN21AE5521.seq Check: 6092 from: 1 to: 6016 pFN21AE5521 6016 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3306 3306 Size: 6016 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3826 278 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3087 3359 1937 Size: 1150 272 4594 Fragments arranged by size: 4594 1150 272 Acc65I G'GTAC_C Cuts at: 3337 3337 Size: 6016 AclI AA'CG_TT Cuts at: 1563 3125 4143 4516 1563 Size: 1562 1018 373 3063 Fragments arranged by size: 3063 1562 1018 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2192 2324 3062 4079 5463 5731 1632 Size: 560 132 738 1017 1384 268 1917 Fragments arranged by size: 1917 1384 1017 738 560 268 132 AflII C'TTAA_G Cuts at: 820 1017 2192 820 Size: 197 1175 4644 Fragments arranged by size: 4644 1175 197 AflIII A'CryG_T Cuts at: 1203 2487 4915 5892 1203 Size: 1284 2428 977 1327 Fragments arranged by size: 2428 1327 1284 977 AgeI A'CCGG_T Cuts at: 4847 4847 Size: 6016 AhdI GACnn_n'nnGTC Cuts at: 4745 4745 Size: 6016 AleI CACnn'nnGTG Cuts at: 1166 2865 1166 Size: 1699 4317 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6016 AlwNI CAG_nnn'CTG Cuts at: 2249 2900 3759 5331 2249 Size: 651 859 1572 2934 Fragments arranged by size: 2934 1572 859 651 ApaLI G'TGCA_C Cuts at: 4073 5229 4073 Size: 1156 4860 ApoI r'AATT_y Cuts at: 1493 2436 3325 3551 3703 5812 1493 Size: 943 889 226 152 2109 1697 Fragments arranged by size: 2109 1697 943 889 226 152 AseI AT'TA_AT Cuts at: 160 1032 4570 160 Size: 872 3538 1606 Fragments arranged by size: 3538 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2277 3341 1870 Size: 73 72 262 1064 4545 Fragments arranged by size: 4545 1064 262 73 72 BamHI G'GATC_C Cuts at: 3346 3713 3346 Size: 367 5649 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3337 4792 618 Size: 325 217 627 1550 1455 1842 Fragments arranged by size: 1842 1550 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2284 3140 3335 1391 Size: 893 856 195 4072 Fragments arranged by size: 4072 893 856 195 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5045 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4207 4241 1903 Size: 34 2270 34 3678 Fragments arranged by size: 3678 2270 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2723 3009 3908 5118 2723 Size: 286 899 1210 3621 Fragments arranged by size: 3621 1210 899 286 BclI T'GATC_A Cuts at: 1548 1548 Size: 6016 BfrBI ATG'CAT Cuts at: 2452 2978 3185 2452 Size: 526 207 5283 Fragments arranged by size: 5283 526 207 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4627 136 Size: 107 122 71 631 605 2955 1525 Fragments arranged by size: 2955 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2335 6011 2335 Size: 3676 2340 BlpI GC'TnA_GC Cuts at: 3433 3433 Size: 6016 Bme1580I G_kGCm'C Cuts at: 1790 2091 4077 5233 1790 Size: 301 1986 1156 2573 Fragments arranged by size: 2573 1986 1156 301 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6016 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3747 4705 442 Size: 416 53 83 399 24 2330 958 1753 Fragments arranged by size: 2330 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6016 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2372 4676 1239 Size: 1133 2304 2579 Fragments arranged by size: 2579 2304 1133 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2770 4141 5006 5304 2770 Size: 1371 865 298 3482 Fragments arranged by size: 3482 1371 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4679 882 Size: 617 3180 2219 Fragments arranged by size: 3180 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3203 3807 493 Size: 711 1999 604 2702 Fragments arranged by size: 2702 1999 711 604 BsaBI GATnn'nnATC Cuts at: 3242 6010 3242 Size: 2768 3248 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3823 4205 4840 275 Size: 53 83 186 936 2290 382 635 1451 Fragments arranged by size: 2290 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4449 4847 5121 5268 5679 4449 Size: 398 274 147 411 4786 Fragments arranged by size: 4786 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5791 Fragments arranged by size: 5791 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6016 BseYI C'CCAG_C Cuts at: 2213 2539 2665 5219 5715 2213 Size: 326 126 2554 496 2514 Fragments arranged by size: 2554 2514 496 326 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5443 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3466 4227 5255 1540 Size: 275 186 1465 761 1028 2301 Fragments arranged by size: 2301 1465 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3140 4077 4162 5233 3140 Size: 937 85 1071 3923 Fragments arranged by size: 3923 1071 937 85 BsmI GAATG_Cn' Cuts at: 3076 3527 3620 3076 Size: 451 93 5472 Fragments arranged by size: 5472 451 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2284 3140 3335 4077 4162 5233 Size: 399 301 193 856 195 742 85 1071 Cuts at: 5233 1391 Size: 2174 Fragments arranged by size: 2174 1071 856 742 399 301 195 193 85 BspEI T'CCGG_A Cuts at: 5679 5679 Size: 6016 BspHI T'CATG_A Cuts at: 3905 5635 3905 Size: 1730 4286 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3306 3371 844 Size: 2462 65 3489 Fragments arranged by size: 3489 2462 65 BsrBI CCG'CTC Cuts at: 1394 1823 3903 1394 Size: 429 2080 3507 Fragments arranged by size: 3507 2080 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4511 4685 65 Size: 1183 3263 174 1396 Fragments arranged by size: 3263 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4660 4847 1700 Size: 110 2850 187 2869 Fragments arranged by size: 2869 2850 187 110 BsrGI T'GTAC_A Cuts at: 96 3257 96 Size: 3161 2855 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6016 BssSI C'ACGA_G Cuts at: 4076 5088 4076 Size: 1012 5004 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6016 BstBI TT'CG_AA Cuts at: 2935 3195 4820 2935 Size: 260 1625 4131 Fragments arranged by size: 4131 1625 260 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6016 BstYI r'GATC_y Cuts at: 1972 2335 2390 3346 3713 4098 4115 5567 5653 Size: 363 55 956 367 385 17 1452 86 Cuts at: 5653 5665 6011 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 956 385 367 363 346 86 55 17 12 BstZ17I GTA'TAC Cuts at: 3088 3088 Size: 6016 BtgI C'CryG_G Cuts at: 513 1065 2800 513 Size: 552 1735 3729 Fragments arranged by size: 3729 1735 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5277 Fragments arranged by size: 5277 683 56 BtsI GCAGTG_nn' Cuts at: 3532 4326 4346 3532 Size: 794 20 5202 Fragments arranged by size: 5202 794 20 ClaI AT'CG_AT Cuts at: 1554 2755 1554 Size: 1201 4815 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5981 DraI TTT'AAA Cuts at: 2535 3320 2535 Size: 785 5231 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5023 809 Size: 507 3707 1802 Fragments arranged by size: 3707 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3463 4352 5981 Size: 54 1425 50 275 18 1633 889 1629 Cuts at: 5981 8 Size: 43 Fragments arranged by size: 1633 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3463 1537 Size: 275 1651 4090 Fragments arranged by size: 4090 1651 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2148 2649 3946 1353 Size: 795 501 1297 3423 Fragments arranged by size: 3423 1297 795 501 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4603 4977 5123 387 Size: 1064 138 3014 374 146 1280 Fragments arranged by size: 3014 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3138 3138 Size: 6016 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2324 2372 3062 4079 4676 5463 Size: 393 560 132 48 690 1017 597 787 Cuts at: 5463 5731 1239 Size: 268 1524 Fragments arranged by size: 1524 1017 787 690 597 560 393 268 132 48 EcoO109I rG'GnC_Cy Cuts at: 2323 2323 Size: 6016 EcoRI G'AATT_C Cuts at: 3325 3703 3325 Size: 378 5638 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2660 2660 Size: 6016 FspI TGC'GCA Cuts at: 4522 4522 Size: 6016 HaeII r_GCGC'y Cuts at: 1678 5163 1678 Size: 3485 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2440 2472 2541 2573 2737 Size: 32 135 32 868 32 69 32 164 Cuts at: 2737 2769 3213 3245 4657 4689 4731 4763 1373 Size: 32 444 32 1412 32 42 32 2626 Fragments arranged by size: 2626 1412 868 444 164 135 69 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3360 3606 669 Size: 988 281 1422 246 3079 Fragments arranged by size: 3079 1422 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6016 HpaI GTT'AAC Cuts at: 3606 3606 Size: 6016 KpnI G_GTAC'C Cuts at: 3341 3341 Size: 6016 MfeI C'AATT_G Cuts at: 3615 3615 Size: 6016 MluI A'CGCG_T Cuts at: 5892 5892 Size: 6016 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2723 5130 5314 1305 Size: 1418 2407 184 2007 Fragments arranged by size: 2407 2007 1418 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4194 Fragments arranged by size: 4194 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2486 2711 2865 2979 3974 4333 4492 Size: 648 1320 225 154 114 995 359 159 Cuts at: 4492 518 Size: 2042 Fragments arranged by size: 2042 1320 995 648 359 225 159 154 114 MspA1I CmG'CkG Cuts at: 1606 2039 3432 4109 5257 5502 1606 Size: 433 1393 677 1148 245 2120 Fragments arranged by size: 2120 1393 1148 677 433 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5464 NdeI CA'TA_TG Cuts at: 387 2164 2509 2980 387 Size: 1777 345 471 3423 Fragments arranged by size: 3423 1777 471 345 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6016 NotI GC'GGCC_GC Cuts at: 3463 3463 Size: 6016 NsiI A_TGCA'T Cuts at: 2454 2980 3187 2454 Size: 526 207 5283 Fragments arranged by size: 5283 526 207 NspI r_CATG'y Cuts at: 1132 1229 2491 3374 4919 1132 Size: 97 1262 883 1545 2229 Fragments arranged by size: 2229 1545 1262 883 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6016 PciI A'CATG_T Cuts at: 2487 4915 2487 Size: 2428 3588 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6016 PmeI GTTT'AAAC Cuts at: 3320 3320 Size: 6016 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4085 4117 5598 5630 4085 Size: 32 1481 32 4471 Fragments arranged by size: 4471 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2323 2323 Size: 6016 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6016 PsiI TTA'TAA Cuts at: 2103 3060 3586 2103 Size: 957 526 4533 Fragments arranged by size: 4533 957 526 PstI C_TGCA'G Cuts at: 830 3055 3368 830 Size: 2225 313 3478 Fragments arranged by size: 3478 2225 313 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6016 PvuII CAG'CTG Cuts at: 2039 2039 Size: 6016 SacI G_AGCT'C Cuts at: 3140 3140 Size: 6016 SalI G'TCGA_C Cuts at: 1936 3358 1936 Size: 1422 4594 SapI GCTCTTCn'nnn_ Cuts at: 2649 2649 Size: 6016 SbfI CC_TGCA'GG Cuts at: 3368 3368 Size: 6016 ScaI AGT'ACT Cuts at: 4264 5976 4264 Size: 1712 4304 SfcI C'TryA_G Cuts at: 826 1045 2919 3051 3364 4499 5180 5371 826 Size: 219 1874 132 313 1135 681 191 1471 Fragments arranged by size: 1874 1471 1135 681 313 219 191 132 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6016 SmaI CCC'GGG Cuts at: 1872 3343 1872 Size: 1471 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2192 2785 4120 5021 5283 820 Size: 197 926 249 593 1335 901 262 1553 Fragments arranged by size: 1553 1335 926 901 593 262 249 197 SnaBI TAC'GTA Cuts at: 493 3807 493 Size: 3314 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6016 SphI G_CATG'C Cuts at: 1132 3374 1132 Size: 2242 3774 SspI AAT'ATT Cuts at: 5 52 3940 5 Size: 47 3888 2081 Fragments arranged by size: 3888 2081 47 StuI AGG'CCT Cuts at: 1517 2478 1517 Size: 961 5055 StyI C'CwwG_G Cuts at: 513 1065 3455 513 Size: 552 2390 3074 Fragments arranged by size: 3074 2390 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2390 4043 4060 4213 4398 4843 325 Size: 1255 810 1653 17 153 185 445 1498 Fragments arranged by size: 1653 1498 1255 810 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2316 3257 4262 Size: 274 80 33 51 1443 339 941 1005 Cuts at: 4262 5974 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1005 941 339 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2566 2598 5976 6008 2566 Size: 32 3378 32 2574 Fragments arranged by size: 3378 2574 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6016 XbaI T'CTAG_A Cuts at: 3352 3352 Size: 6016 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6016 XmaI C'CCGG_G Cuts at: 1870 3341 1870 Size: 1471 4545 XmnI GAAnn'nnTTC Cuts at: 2337 2436 2643 4145 2337 Size: 99 207 1502 4208 Fragments arranged by size: 4208 1502 207 99 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3824 276 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI