(Linear) (Six-base) MAPSORT of: KIEE5521.seq Check: 7515 from: 1 to: 1311 KIEE5521.orf With 182 enzymes: SgfI * January 29, 2014 12:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1301 1311 Size: 1301 10 AccI GT'mk_AC Cuts at: 0 1082 1311 Size: 1082 229 AclI AA'CG_TT Cuts at: 0 1120 1311 Size: 1120 191 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 319 1057 1311 Size: 187 132 738 254 Fragments arranged by size: 738 254 187 132 AflII C'TTAA_G Cuts at: 0 187 1311 Size: 187 1124 AflIII A'CryG_T Cuts at: 0 482 1311 Size: 482 829 AleI CACnn'nnGTG Cuts at: 0 860 1311 Size: 860 451 AlwNI CAG_nnn'CTG Cuts at: 0 244 895 1311 Size: 244 651 416 Fragments arranged by size: 651 416 244 ApoI r'AATT_y Cuts at: 0 431 1311 Size: 431 880 AvaI C'yCGr_G Cuts at: 0 10 272 1311 Size: 10 262 1039 Fragments arranged by size: 1039 262 10 BanII G_rGCy'C Cuts at: 0 279 1135 1311 Size: 279 856 176 Fragments arranged by size: 856 279 176 BciVI GTATCCnnnnn_n' Cuts at: 0 718 1004 1311 Size: 718 286 307 Fragments arranged by size: 718 307 286 BfrBI ATG'CAT Cuts at: 0 447 973 1180 1311 Size: 447 526 207 131 Fragments arranged by size: 526 447 207 131 BglII A'GATC_T Cuts at: 0 330 1311 Size: 330 981 Bme1580I G_kGCm'C Cuts at: 0 86 1311 Size: 86 1225 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 367 1311 Size: 367 944 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 765 1311 Size: 765 546 BsaAI yAC'GTr Cuts at: 0 1198 1311 Size: 1198 113 BsaBI GATnn'nnATC Cuts at: 0 1237 1311 Size: 1237 74 BseYI C'CCAG_C Cuts at: 0 208 534 660 1311 Size: 208 326 126 651 Fragments arranged by size: 651 326 208 126 BsiHKAI G_wGCw'C Cuts at: 0 1135 1311 Size: 1135 176 BsmI GAATG_Cn' Cuts at: 0 1071 1311 Size: 1071 240 Bsp1286I G_dGCh'C Cuts at: 0 86 279 1135 1311 Size: 86 193 856 176 Fragments arranged by size: 856 193 176 86 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1301 1311 Size: 1301 10 BsrGI T'GTAC_A Cuts at: 0 1252 1311 Size: 1252 59 BstBI TT'CG_AA Cuts at: 0 930 1190 1311 Size: 930 260 121 Fragments arranged by size: 930 260 121 BstYI r'GATC_y Cuts at: 0 330 385 1311 Size: 330 55 926 Fragments arranged by size: 926 330 55 BstZ17I GTA'TAC Cuts at: 0 1083 1311 Size: 1083 228 BtgI C'CryG_G Cuts at: 0 795 1311 Size: 795 516 ClaI AT'CG_AT Cuts at: 0 750 1311 Size: 750 561 DraI TTT'AAA Cuts at: 0 530 1311 Size: 530 781 EarI CTCTTCn'nnn_ Cuts at: 0 143 644 1311 Size: 143 501 667 Fragments arranged by size: 667 501 143 EcoICRI GAG'CTC Cuts at: 0 1133 1311 Size: 1133 178 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 319 367 1057 1311 Size: 187 132 48 690 254 Fragments arranged by size: 690 254 187 132 48 EcoO109I rG'GnC_Cy Cuts at: 0 318 1311 Size: 318 993 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 655 1311 Size: 655 656 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 435 467 536 568 732 764 1208 1240 Size: 435 32 69 32 164 32 444 32 Cuts at: 1240 1311 Size: 71 Fragments arranged by size: 444 435 164 71 69 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 718 1311 Size: 718 593 MslI CAynn'nnrTG Cuts at: 0 481 706 860 974 1311 Size: 481 225 154 114 337 Fragments arranged by size: 481 337 225 154 114 MspA1I CmG'CkG Cuts at: 0 34 1311 Size: 34 1277 NdeI CA'TA_TG Cuts at: 0 159 504 975 1311 Size: 159 345 471 336 Fragments arranged by size: 471 345 336 159 NsiI A_TGCA'T Cuts at: 0 449 975 1182 1311 Size: 449 526 207 129 Fragments arranged by size: 526 449 207 129 NspI r_CATG'y Cuts at: 0 486 1311 Size: 486 825 PciI A'CATG_T Cuts at: 0 482 1311 Size: 482 829 PpuMI rG'GwC_Cy Cuts at: 0 318 1311 Size: 318 993 PsiI TTA'TAA Cuts at: 0 98 1055 1311 Size: 98 957 256 Fragments arranged by size: 957 256 98 PstI C_TGCA'G Cuts at: 0 1050 1311 Size: 1050 261 PvuII CAG'CTG Cuts at: 0 34 1311 Size: 34 1277 SacI G_AGCT'C Cuts at: 0 1135 1311 Size: 1135 176 SapI GCTCTTCn'nnn_ Cuts at: 0 644 1311 Size: 644 667 SfcI C'TryA_G Cuts at: 0 914 1046 1311 Size: 914 132 265 Fragments arranged by size: 914 265 132 SmlI C'TyrA_G Cuts at: 0 187 780 1311 Size: 187 593 531 Fragments arranged by size: 593 531 187 StuI AGG'CCT Cuts at: 0 473 1311 Size: 473 838 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 385 1311 Size: 385 926 TatI w'GTAC_w Cuts at: 0 311 1252 1311 Size: 311 941 59 Fragments arranged by size: 941 311 59 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 561 593 1311 Size: 561 32 718 Fragments arranged by size: 718 561 32 XmnI GAAnn'nnTTC Cuts at: 0 332 431 638 1311 Size: 332 99 207 673 Fragments arranged by size: 673 332 207 99 Enzymes that do cut: AarI AccI AclI AcuI AflII AflIII AleI AlwNI ApoI AvaI BanII BciVI BfrBI BglII Bme1580I BpmI BpuEI BsaAI BsaBI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrGI BstBI BstYI BstZ17I BtgI ClaI DraI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I MmeI MslI MspA1I NdeI NsiI NspI PciI PpuMI PsiI PstI PvuII SacI SapI SfcI SmlI StuI TaqII TatI TstI XmnI Enzymes that do not cut: AatII Acc65I AfeI AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BclI BglI BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI Bsu36I BtgZI BtsI CspCI DraIII DrdI EaeI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI