(Circular) (Six-base) MAPSORT of: pFN21AE4516.seq Check: 4112 from: 1 to: 6355 pFN21AE4516 6355 bp With 182 enzymes: SgfI * March 28, 2012 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2020 2760 2020 Size: 740 5615 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4165 278 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2691 3335 3698 1937 Size: 754 644 363 4594 Fragments arranged by size: 4594 754 644 363 Acc65I G'GTAC_C Cuts at: 3676 3676 Size: 6355 AclI AA'CG_TT Cuts at: 1563 4482 4855 1563 Size: 2919 373 3063 Fragments arranged by size: 3063 2919 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4418 5802 6070 1632 Size: 2786 1384 268 1917 Fragments arranged by size: 2786 1917 1384 268 AfeI AGC'GCT Cuts at: 3499 3499 Size: 6355 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6158 AflIII A'CryG_T Cuts at: 1203 5254 6231 1203 Size: 4051 977 1327 Fragments arranged by size: 4051 1327 977 AgeI A'CCGG_T Cuts at: 5186 5186 Size: 6355 AhdI GACnn_n'nnGTC Cuts at: 5084 5084 Size: 6355 AleI CACnn'nnGTG Cuts at: 1166 2072 1166 Size: 906 5449 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3241 1184 Size: 2057 4298 AlwNI CAG_nnn'CTG Cuts at: 2761 3538 3559 4098 5670 2761 Size: 777 21 539 1572 3446 Fragments arranged by size: 3446 1572 777 539 21 ApaI G_GGCC'C Cuts at: 2401 2866 2401 Size: 465 5890 ApaLI G'TGCA_C Cuts at: 4412 5568 4412 Size: 1156 5199 ApoI r'AATT_y Cuts at: 1493 3664 3890 4042 6151 1493 Size: 2171 226 152 2109 1697 Fragments arranged by size: 2171 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4909 160 Size: 872 3877 1606 Fragments arranged by size: 3877 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 AvaI C'yCGr_G Cuts at: 1870 1943 2208 2587 3399 3680 1870 Size: 73 265 379 812 281 4545 Fragments arranged by size: 4545 812 379 281 265 73 BamHI G'GATC_C Cuts at: 3685 4052 3685 Size: 367 5988 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2672 2745 3676 5131 618 Size: 325 217 627 885 73 931 1455 1842 Fragments arranged by size: 1842 1455 931 885 627 325 217 73 BanII G_rGCy'C Cuts at: 1391 2316 2401 2866 3422 3447 3674 1391 Size: 925 85 465 556 25 227 4072 Fragments arranged by size: 4072 925 556 465 227 85 25 BbeI G_GCGC'C Cuts at: 2749 2749 Size: 6355 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5384 BbvCI CC'TCA_GC Cuts at: 2174 3631 2174 Size: 1457 4898 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4546 4580 1903 Size: 34 2609 34 3678 Fragments arranged by size: 3678 2609 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3360 4247 5457 3360 Size: 887 1210 4258 Fragments arranged by size: 4258 1210 887 BclI T'GATC_A Cuts at: 1548 1548 Size: 6355 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2743 3200 4966 Size: 107 122 71 631 605 1071 457 1766 Cuts at: 4966 136 Size: 1525 Fragments arranged by size: 1766 1525 1071 631 605 457 122 107 71 BglII A'GATC_T Cuts at: 6350 6350 Size: 6355 BlpI GC'TnA_GC Cuts at: 3772 3772 Size: 6355 Bme1580I G_kGCm'C Cuts at: 1790 2401 2675 2739 2866 4416 5572 1790 Size: 611 274 64 127 1550 1156 2573 Fragments arranged by size: 2573 1550 1156 611 274 127 64 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6355 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2410 3348 4086 Size: 416 53 83 399 24 993 938 738 Cuts at: 4086 5044 442 Size: 958 1753 Fragments arranged by size: 1753 993 958 938 738 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6355 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 3581 5015 1239 Size: 974 1368 1434 2579 Fragments arranged by size: 2579 1434 1368 974 Bpu10I CC'TnA_GC Cuts at: 2174 3631 2174 Size: 1457 4898 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4480 5345 5643 4480 Size: 865 298 5192 Fragments arranged by size: 5192 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2770 3515 5018 882 Size: 617 1271 745 1503 2219 Fragments arranged by size: 2219 1503 1271 745 617 BsaAI yAC'GTr Cuts at: 493 1204 4146 493 Size: 711 2942 2702 Fragments arranged by size: 2942 2702 711 BsaBI GATnn'nnATC Cuts at: 6349 6349 Size: 6355 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2746 4162 4544 5179 Size: 53 83 186 936 1213 1416 382 635 Cuts at: 5179 275 Size: 1451 Fragments arranged by size: 1451 1416 1213 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4788 5186 5460 5607 6018 4788 Size: 398 274 147 411 5125 Fragments arranged by size: 5125 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2439 2469 2819 2849 1178 Size: 30 165 30 1036 30 350 30 4684 Fragments arranged by size: 4684 1036 350 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2573 2821 2824 1186 Size: 1387 248 3 4717 Fragments arranged by size: 4717 1387 248 3 BseYI C'CCAG_C Cuts at: 3289 5558 6054 3289 Size: 2269 496 3590 Fragments arranged by size: 3590 2269 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2302 3189 3572 1163 Size: 573 566 887 383 3946 Fragments arranged by size: 3946 887 573 566 383 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2047 3805 4566 5594 1540 Size: 275 186 46 1758 761 1028 2301 Fragments arranged by size: 2301 1758 1028 761 275 186 46 BsiHKAI G_wGCw'C Cuts at: 2390 3447 3603 4416 4501 5572 2390 Size: 1057 156 813 85 1071 3173 Fragments arranged by size: 3173 1071 1057 813 156 85 BsmI GAATG_Cn' Cuts at: 3866 3959 3866 Size: 93 6262 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2316 2390 2401 2675 2739 2866 3422 Size: 399 526 74 11 274 64 127 556 Cuts at: 3422 3447 3603 3674 4416 4501 5572 1391 Size: 25 156 71 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 556 526 399 274 156 127 85 74 71 64 25 11 BspEI T'CCGG_A Cuts at: 6018 6018 Size: 6355 BspHI T'CATG_A Cuts at: 4244 5974 4244 Size: 1730 4625 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2020 2520 2543 2760 3090 3147 3710 844 Size: 1176 500 23 217 330 57 563 3489 Fragments arranged by size: 3489 1176 563 500 330 217 57 23 BsrBI CCG'CTC Cuts at: 1394 1823 4242 1394 Size: 429 2419 3507 Fragments arranged by size: 3507 2419 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4850 5024 65 Size: 1183 3602 174 1396 Fragments arranged by size: 3602 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3186 4999 5186 1700 Size: 110 1376 1813 187 2869 Fragments arranged by size: 2869 1813 1376 187 110 BsrGI T'GTAC_A Cuts at: 96 2424 96 Size: 2328 4027 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6355 BssSI C'ACGA_G Cuts at: 4415 5427 4415 Size: 1012 5343 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6355 BstBI TT'CG_AA Cuts at: 5159 5159 Size: 6355 BstEII G'GTnAC_C Cuts at: 1184 2065 1184 Size: 881 5474 BstXI CCAn_nnnn'nTGG Cuts at: 3154 3154 Size: 6355 BstYI r'GATC_y Cuts at: 1972 2058 2331 3685 4052 4437 4454 5906 5992 Size: 86 273 1354 367 385 17 1452 86 Cuts at: 5992 6004 6350 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1354 385 367 346 273 86 86 17 12 BstZ17I GTA'TAC Cuts at: 3336 3336 Size: 6355 Bsu36I CC'TnA_GG Cuts at: 2858 2858 Size: 6355 BtgI C'CryG_G Cuts at: 513 1065 2004 2451 2958 2982 3393 513 Size: 552 939 447 507 24 411 3475 Fragments arranged by size: 3475 939 552 507 447 411 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5616 Fragments arranged by size: 5616 683 56 BtsI GCAGTG_nn' Cuts at: 2124 2146 2264 3206 3536 3620 3871 4665 4685 Size: 22 118 942 330 84 251 794 20 Cuts at: 4685 2124 Size: 3794 Fragments arranged by size: 3794 942 794 330 251 118 84 22 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6355 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3037 3072 529 Size: 35 2473 35 3812 Fragments arranged by size: 3812 2473 35 35 DraI TTT'AAA Cuts at: 3659 3659 Size: 6355 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2524 5362 809 Size: 507 1208 2838 1802 Fragments arranged by size: 2838 1802 1208 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2044 2979 3489 Size: 54 1425 50 275 18 214 935 510 Cuts at: 3489 3802 4691 6320 8 Size: 313 889 1629 43 Fragments arranged by size: 1629 1425 935 889 510 313 275 214 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2044 3802 1537 Size: 275 232 1758 4090 Fragments arranged by size: 4090 1758 275 232 EarI CTCTTCn'nnn_ Cuts at: 1353 3023 3470 4285 1353 Size: 1670 447 815 3423 Fragments arranged by size: 3423 1670 815 447 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2999 4942 5316 5462 387 Size: 1064 138 1410 1943 374 146 1280 Fragments arranged by size: 1943 1410 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3445 3445 Size: 6355 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 3581 4418 5015 5802 6070 1239 Size: 393 581 1368 837 597 787 268 1524 Fragments arranged by size: 1524 1368 837 787 597 581 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2027 2839 3113 2027 Size: 812 274 5269 Fragments arranged by size: 5269 812 274 EcoO109I rG'GnC_Cy Cuts at: 2377 2397 2398 2532 2862 2863 3556 2377 Size: 20 1 134 330 1 693 5176 Fragments arranged by size: 5176 693 330 134 20 1 1 EcoRI G'AATT_C Cuts at: 3664 4042 3664 Size: 378 5977 FspI TGC'GCA Cuts at: 2539 4861 2539 Size: 2322 4033 HaeII r_GCGC'y Cuts at: 1678 2749 3501 5502 1678 Size: 1071 752 2001 2531 Fragments arranged by size: 2531 2001 1071 752 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2086 2118 3087 3119 4996 Size: 32 135 32 514 32 969 32 1877 Cuts at: 4996 5028 5070 5102 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1877 969 514 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3699 3945 669 Size: 988 281 1761 246 3079 Fragments arranged by size: 3079 1761 988 281 246 HindIII A'AGCT_T Cuts at: 748 3149 748 Size: 2401 3954 HpaI GTT'AAC Cuts at: 3945 3945 Size: 6355 KasI G'GCGC_C Cuts at: 2745 2745 Size: 6355 KpnI G_GTAC'C Cuts at: 3680 3680 Size: 6355 MfeI C'AATT_G Cuts at: 3954 3954 Size: 6355 MluI A'CGCG_T Cuts at: 6231 6231 Size: 6355 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5469 5653 1305 Size: 4164 184 2007 Fragments arranged by size: 4164 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4533 Fragments arranged by size: 4533 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 3380 4313 4672 4831 518 Size: 648 906 1308 933 359 159 2042 Fragments arranged by size: 2042 1308 933 906 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2410 2522 2823 3771 4448 5596 5841 1606 Size: 804 112 301 948 677 1148 245 2120 Fragments arranged by size: 2120 1148 948 804 677 301 245 112 NaeI GCC'GGC Cuts at: 3188 3188 Size: 6355 NarI GG'CG_CC Cuts at: 2746 2746 Size: 6355 NcoI C'CATG_G Cuts at: 513 1065 2004 2451 2958 2982 3393 513 Size: 552 939 447 507 24 411 3475 Fragments arranged by size: 3475 939 552 507 447 411 24 NdeI CA'TA_TG Cuts at: 387 387 Size: 6355 NgoMIV G'CCGG_C Cuts at: 3186 3186 Size: 6355 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6355 NotI GC'GGCC_GC Cuts at: 3802 3802 Size: 6355 NspI r_CATG'y Cuts at: 1132 1229 3713 5258 1132 Size: 97 2484 1545 2229 Fragments arranged by size: 2484 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3178 3526 1348 Size: 1830 348 4177 Fragments arranged by size: 4177 1830 348 PciI A'CATG_T Cuts at: 5254 5254 Size: 6355 PflMI CCAn_nnn'nTGG Cuts at: 1108 3039 1108 Size: 1931 4424 PfoI T'CCnGG_A Cuts at: 2837 2837 Size: 6355 PmeI GTTT'AAAC Cuts at: 3659 3659 Size: 6355 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4424 4456 5937 5969 4424 Size: 32 1481 32 4810 Fragments arranged by size: 4810 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2377 2532 3556 2377 Size: 155 1024 5176 Fragments arranged by size: 5176 1024 155 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6355 PsiI TTA'TAA Cuts at: 3925 3925 Size: 6355 PspOMI G'GGCC_C Cuts at: 2397 2862 2397 Size: 465 5890 PstI C_TGCA'G Cuts at: 830 2028 2118 2517 2757 2871 2946 3087 3627 Size: 1198 90 399 240 114 75 141 540 Cuts at: 3627 3707 830 Size: 80 3478 Fragments arranged by size: 3478 1198 540 399 240 141 114 90 80 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6355 PvuII CAG'CTG Cuts at: 2410 2522 2410 Size: 112 6243 SacI G_AGCT'C Cuts at: 3447 3447 Size: 6355 SalI G'TCGA_C Cuts at: 1936 3697 1936 Size: 1761 4594 SbfI CC_TGCA'GG Cuts at: 3087 3707 3087 Size: 620 5735 ScaI AGT'ACT Cuts at: 4603 6315 4603 Size: 1712 4643 SfcI C'TryA_G Cuts at: 826 1045 2024 2114 2513 2692 2753 2867 2942 Size: 219 979 90 399 179 61 114 75 Cuts at: 2942 3083 3623 3703 4838 5519 5710 826 Size: 141 540 80 1135 681 191 1471 Fragments arranged by size: 1471 1135 979 681 540 399 219 191 179 141 114 90 80 75 61 SfoI GGC'GCC Cuts at: 2747 2747 Size: 6355 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6355 SmaI CCC'GGG Cuts at: 1872 2210 3682 1872 Size: 338 1472 4545 Fragments arranged by size: 4545 1472 338 SmlI C'TyrA_G Cuts at: 820 1017 1943 4459 5360 5622 820 Size: 197 926 2516 901 262 1553 Fragments arranged by size: 2516 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4146 493 Size: 3653 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6355 SphI G_CATG'C Cuts at: 1132 3713 1132 Size: 2581 3774 SspI AAT'ATT Cuts at: 5 52 4279 5 Size: 47 4227 2081 Fragments arranged by size: 4227 2081 47 StuI AGG'CCT Cuts at: 1517 3629 1517 Size: 2112 4243 StyI C'CwwG_G Cuts at: 513 1065 2004 2451 2958 2982 3393 3794 513 Size: 552 939 447 507 24 411 401 3074 Fragments arranged by size: 3074 939 552 507 447 411 401 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2566 4382 4399 4552 4737 5182 325 Size: 1255 986 1816 17 153 185 445 1498 Fragments arranged by size: 1816 1498 1255 986 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2424 4601 6313 Size: 274 80 33 51 1443 447 2177 1712 Cuts at: 6313 96 Size: 138 Fragments arranged by size: 2177 1712 1443 447 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2369 2401 2439 2471 2541 2573 2819 2851 6315 Size: 32 38 32 70 32 246 32 3464 Cuts at: 6315 6347 2369 Size: 32 2377 Fragments arranged by size: 3464 2377 246 70 38 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6355 XbaI T'CTAG_A Cuts at: 3691 3691 Size: 6355 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2917 3122 3400 2917 Size: 205 278 5872 Fragments arranged by size: 5872 278 205 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6355 XmaI C'CCGG_G Cuts at: 1870 2208 3680 1870 Size: 338 1472 4545 Fragments arranged by size: 4545 1472 338 XmnI GAAnn'nnTTC Cuts at: 4484 4484 Size: 6355 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4163 276 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI