Restriction map for
pFN21AE4451
(Circular) (Six-base) MAPSORT of: pFN21AE4451.seq Check: 3673 from: 1 to: 6109
pFN21AE4451 6109 bp
With 182 enzymes: SgfI *
March 28, 2012 13:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3919 278
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2625 3114 3452 1937
Size: 688 489 338 4594
Fragments arranged by size:
4594 688 489 338
Acc65I G'GTAC_C
Cuts at: 3430 3430
Size: 6109
AclI AA'CG_TT
Cuts at: 1563 4236 4609 1563
Size: 2673 373 3063
Fragments arranged by size:
3063 2673 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2361 2531 2847 4172 5556 5824 1632
Size: 729 170 316 1325 1384 268 1917
Fragments arranged by size:
1917 1384 1325 729 316 268 170
AflII C'TTAA_G
Cuts at: 820 1017 2156 820
Size: 197 1139 4773
Fragments arranged by size:
4773 1139 197
AflIII A'CryG_T
Cuts at: 1203 2368 5008 5985 1203
Size: 1165 2640 977 1327
Fragments arranged by size:
2640 1327 1165 977
AgeI A'CCGG_T
Cuts at: 4940 4940
Size: 6109
AhdI GACnn_n'nnGTC
Cuts at: 4838 4838
Size: 6109
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6109
AlwNI CAG_nnn'CTG
Cuts at: 2384 3852 5424 2384
Size: 1468 1572 3069
Fragments arranged by size:
3069 1572 1468
ApaI G_GGCC'C
Cuts at: 2028 2028
Size: 6109
ApaLI G'TGCA_C
Cuts at: 4166 5322 4166
Size: 1156 4953
ApoI r'AATT_y
Cuts at: 1493 2228 2564 3266 3418 3644 3796 5905 1493
Size: 735 336 702 152 226 152 2109 1697
Fragments arranged by size:
2109 1697 735 702 336 226 152 152
AseI AT'TA_AT
Cuts at: 160 1032 3377 4663 160
Size: 872 2345 1286 1606
Fragments arranged by size:
2345 1606 1286 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
AvaI C'yCGr_G
Cuts at: 1870 1943 3434 1870
Size: 73 1491 4545
Fragments arranged by size:
4545 1491 73
BamHI G'GATC_C
Cuts at: 3439 3806 3439
Size: 367 5742
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3049 3430 4885 618
Size: 325 217 627 1262 381 1455 1842
Fragments arranged by size:
1842 1455 1262 627 381 325 217
BanII G_rGCy'C
Cuts at: 1391 2028 2796 3428 1391
Size: 637 768 632 4072
Fragments arranged by size:
4072 768 637 632
BbeI G_GCGC'C
Cuts at: 3053 3053
Size: 6109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2809 2878 2992 928
Size: 971 910 69 114 4045
Fragments arranged by size:
4045 971 910 114 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4300 4334 1903
Size: 34 2363 34 3678
Fragments arranged by size:
3678 2363 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2724 4001 5211 2724
Size: 1277 1210 3622
Fragments arranged by size:
3622 1277 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4720 136
Size: 107 122 71 631 605 337 2711 1525
Fragments arranged by size:
2711 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6104 6104
Size: 6109
BlpI GC'TnA_GC
Cuts at: 3526 3526
Size: 6109
Bme1580I G_kGCm'C
Cuts at: 1790 2028 4170 5326 1790
Size: 238 2142 1156 2573
Fragments arranged by size:
2573 2142 1156 238
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6109
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3840 4798 442
Size: 416 53 83 399 24 2423 958 1753
Fragments arranged by size:
2423 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4769 1239
Size: 3530 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4234 5099 5397 4234
Size: 865 298 4946
Fragments arranged by size:
4946 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4772 882
Size: 617 3273 2219
Fragments arranged by size:
3273 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2369 3900 493
Size: 711 1165 1531 2702
Fragments arranged by size:
2702 1531 1165 711
BsaBI GATnn'nnATC
Cuts at: 6103 6103
Size: 6109
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3050 3916 4298 4933
Size: 53 83 186 936 1517 866 382 635
Cuts at: 4933 275
Size: 1451
Fragments arranged by size:
1517 1451 936 866 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4542 4940 5214 5361 5772 4542
Size: 398 274 147 411 4879
Fragments arranged by size:
4879 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2470 2500 1178
Size: 30 165 30 1067 30 4787
Fragments arranged by size:
4787 1067 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6109
BseYI C'CCAG_C
Cuts at: 5312 5808 5312
Size: 496 5613
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2842 1163
Size: 573 1106 4430
Fragments arranged by size:
4430 1106 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3559 4320 5348 1540
Size: 275 186 1558 761 1028 2301
Fragments arranged by size:
2301 1558 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4170 4255 5326 4170
Size: 85 1071 4953
Fragments arranged by size:
4953 1071 85
BsmI GAATG_Cn'
Cuts at: 3620 3713 3620
Size: 93 6016
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2796 3428 4170 4255 5326 1391
Size: 399 238 768 632 742 85 1071 2174
Fragments arranged by size:
2174 1071 768 742 632 399 238 85
BspEI T'CCGG_A
Cuts at: 5772 5772
Size: 6109
BspHI T'CATG_A
Cuts at: 3225 3998 5728 3225
Size: 773 1730 3606
Fragments arranged by size:
3606 1730 773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3464 844
Size: 2620 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3996 1394
Size: 429 2173 3507
Fragments arranged by size:
3507 2173 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4604 4778 65
Size: 1183 3356 174 1396
Fragments arranged by size:
3356 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2491 4753 4940 1700
Size: 110 681 2262 187 2869
Fragments arranged by size:
2869 2262 681 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6109
BssHII G'CGCG_C
Cuts at: 1925 2031 2100 1925
Size: 106 69 5934
Fragments arranged by size:
5934 106 69
BssSI C'ACGA_G
Cuts at: 4169 5181 4169
Size: 1012 5097
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6109
BstBI TT'CG_AA
Cuts at: 4913 4913
Size: 6109
BstEII G'GTnAC_C
Cuts at: 1184 2992 1184
Size: 1808 4301
BstYI r'GATC_y
Cuts at: 1972 3329 3439 3806 4191 4208 5660 5746 5758
Size: 1357 110 367 385 17 1452 86 12
Cuts at: 5758 6104 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1357 385 367 346 110 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3045 513
Size: 552 939 1041 3577
Fragments arranged by size:
3577 1041 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5370
Fragments arranged by size:
5370 683 56
BtsI GCAGTG_nn'
Cuts at: 3625 4419 4439 3625
Size: 794 20 5295
Fragments arranged by size:
5295 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6074
DraI TTT'AAA
Cuts at: 3413 3413
Size: 6109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5116 809
Size: 507 3800 1802
Fragments arranged by size:
3800 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3556 4445 6074
Size: 54 1425 50 275 18 1726 889 1629
Cuts at: 6074 8
Size: 43
Fragments arranged by size:
1726 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3556 1537
Size: 275 1744 4090
Fragments arranged by size:
4090 1744 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2898 4039 1353
Size: 1545 1141 3423
Fragments arranged by size:
3423 1545 1141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 4696 5070 5216 387
Size: 1064 138 438 2669 374 146 1280
Fragments arranged by size:
2669 1280 1064 438 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2361 2531 2847 4172 4769 5556 5824
Size: 393 729 170 316 1325 597 787 268
Cuts at: 5824 1239
Size: 1524
Fragments arranged by size:
1524 1325 787 729 597 393 316 268
170
EcoO109I rG'GnC_Cy
Cuts at: 2024 2025 2024
Size: 1 6108
EcoRI G'AATT_C
Cuts at: 3418 3796 3418
Size: 378 5731
FspI TGC'GCA
Cuts at: 4615 4615
Size: 6109
HaeII r_GCGC'y
Cuts at: 1678 2074 2749 3053 5256 1678
Size: 396 675 304 2203 2531
Fragments arranged by size:
2531 2203 675 396 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4750 4782 4824 4856 1373
Size: 32 135 32 3178 32 42 32 2626
Fragments arranged by size:
3178 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3453 3699 669
Size: 988 281 1515 246 3079
Fragments arranged by size:
3079 1515 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6109
HpaI GTT'AAC
Cuts at: 3699 3699
Size: 6109
KasI G'GCGC_C
Cuts at: 3049 3049
Size: 6109
KpnI G_GTAC'C
Cuts at: 3434 3434
Size: 6109
MfeI C'AATT_G
Cuts at: 2249 3321 3708 2249
Size: 1072 387 4650
Fragments arranged by size:
4650 1072 387
MluI A'CGCG_T
Cuts at: 5985 5985
Size: 6109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3307 5223 5407 1305
Size: 2002 1916 184 2007
Fragments arranged by size:
2007 2002 1916 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4287
Fragments arranged by size:
4287 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2208 4067 4426 4585 518
Size: 648 1042 1859 359 159 2042
Fragments arranged by size:
2042 1859 1042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 2703 3525 4202 5350 5595 1606
Size: 453 644 822 677 1148 245 2120
Fragments arranged by size:
2120 1148 822 677 644 453 245
NarI GG'CG_CC
Cuts at: 3050 3050
Size: 6109
NcoI C'CATG_G
Cuts at: 513 1065 2004 3045 513
Size: 552 939 1041 3577
Fragments arranged by size:
3577 1041 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6109
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6109
NotI GC'GGCC_GC
Cuts at: 3556 3556
Size: 6109
NspI r_CATG'y
Cuts at: 1132 1229 3467 5012 1132
Size: 97 2238 1545 2229
Fragments arranged by size:
2238 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6109
PciI A'CATG_T
Cuts at: 5008 5008
Size: 6109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6109
PmeI GTTT'AAAC
Cuts at: 3413 3413
Size: 6109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 5691 5723 4178
Size: 32 1481 32 4564
Fragments arranged by size:
4564 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6109
PsiI TTA'TAA
Cuts at: 2777 3360 3679 2777
Size: 583 319 5207
Fragments arranged by size:
5207 583 319
PspOMI G'GGCC_C
Cuts at: 2024 2024
Size: 6109
PstI C_TGCA'G
Cuts at: 830 3461 830
Size: 2631 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6109
PvuII CAG'CTG
Cuts at: 2059 2703 2059
Size: 644 5465
SalI G'TCGA_C
Cuts at: 1936 3451 1936
Size: 1515 4594
SbfI CC_TGCA'GG
Cuts at: 3461 3461
Size: 6109
ScaI AGT'ACT
Cuts at: 2868 4357 6069 2868
Size: 1489 1712 2908
Fragments arranged by size:
2908 1712 1489
SfcI C'TryA_G
Cuts at: 826 1045 3457 4592 5273 5464 826
Size: 219 2412 1135 681 191 1471
Fragments arranged by size:
2412 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3051 3051
Size: 6109
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6109
SmaI CCC'GGG
Cuts at: 1872 3436 1872
Size: 1564 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2156 4213 5114 5376 820
Size: 197 926 213 2057 901 262 1553
Fragments arranged by size:
2057 1553 926 901 262 213 197
SnaBI TAC'GTA
Cuts at: 493 3900 493
Size: 3407 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6109
SphI G_CATG'C
Cuts at: 1132 3467 1132
Size: 2335 3774
SspI AAT'ATT
Cuts at: 5 52 4033 5
Size: 47 3981 2081
Fragments arranged by size:
3981 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6109
StyI C'CwwG_G
Cuts at: 513 1065 2004 3045 3548 513
Size: 552 939 1041 503 3074
Fragments arranged by size:
3074 1041 939 552 503
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4136 4153 4306 4491 4936 325
Size: 1255 2556 17 153 185 445 1498
Fragments arranged by size:
2556 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2809 2866 4355
Size: 274 80 33 51 1443 832 57 1489
Cuts at: 4355 6067 96
Size: 1712 138
Fragments arranged by size:
1712 1489 1443 832 274 138 80 57
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2470 2502 6069 6101 2470
Size: 32 3567 32 2478
Fragments arranged by size:
3567 2478 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2384 2980 1316
Size: 1068 596 4445
Fragments arranged by size:
4445 1068 596
XbaI T'CTAG_A
Cuts at: 2608 3170 3445 2608
Size: 562 275 5272
Fragments arranged by size:
5272 562 275
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3052 3052
Size: 6109
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6109
XmaI C'CCGG_G
Cuts at: 1870 3434 1870
Size: 1564 4545
XmnI GAAnn'nnTTC
Cuts at: 4238 4238
Size: 6109
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3917 276
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI