################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII AclI AcyI AflII AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI AspEI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII Eam1105I Eco47III Eco81I EcoRI EcoRV EcoT22I FseI FspI Hin1I HpaI KasI KspI MamI MfeI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 722 GGTACC G^GTACC AccIII 620 TCCGGA T^CCGGA AcsI 247 AAATTC [GA]^AATT[CT] AlwI 906 GATCC GGATC(4/5) ApoI 247 AAATTC [GA]^AATT[CT] Asp700I 735 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 722 GGTACC G^GTACC AvaI 827 CCCGGG C^[CT]CG[GA]G BalI 1130 TGGCCA TGG^CCA BcgI 341 CGAGCGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 487 GTATCC GTATCC(6/5) BclI 1166 TGATCA T^GATCA BsaAI 1261 TACGTG [CT]AC^GT[GA] BseAI 620 TCCGGA T^CCGGA BsgI 703 CTGCAC GTGCAG(16/14) BsmFI 585 GGGAC GGGAC(10/14) BsoBI 827 CCCGGG C^[CT]CG[GA]G BspEI 620 TCCGGA T^CCGGA BstYI 905 AGATCC [GA]^GATC[CT] EarI 624 GAAGAG CTCTTC(1/4) FbaI 1166 TGATCA T^GATCA HaeII 1269 GGCGCT [GA]GCGC^[CT] HgaI 578 GCGTC GACGC(5/10) HindIII 361 AAGCTT A^AGCTT KpnI 722 GGTACC GGTAC^C Ksp632I 624 GAAGAG CTCTTC(1/4) MflI 905 AGATCC [GA]^GATC[CT] MluI 809 ACGCGT A^CGCGT MluNI 1130 TGGCCA TGG^CCA MroI 620 TCCGGA T^CCGGA MscI 1130 TGGCCA TGG^CCA MseI 764 TTAA T^TAA MslI 41 CACGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 116 GCCGGC GCC^GGC NgoMIV 116 GCCGGC G^CCGGC PflMI 540 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 1291 GAGCTC GAGCT^C SmaI 827 CCCGGG CCC^GGG Tru9I 764 TTAA T^TAA Van91I 540 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1211 CCAAAGGCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 905 AGATCC [GA]^GATC[CT] XmaCI 827 CCCGGG C^CCGGG XmaI 827 CCCGGG C^CCGGG XmnI 735 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 99 GTCGAC GT^[AC][GT]AC 1123 GTCGAC AccII 810 CGCG CG^CG 1305 CGCG AfaI 723 GTAC GT^AC 1260 GTAC AflIII 809 ACGCGT A^C[GA][CT]GT 1262 ACGTGT AgeI 767 ACCGGT A^CCGGT 938 ACCGGT ApaI 595 GGGCCC GGGCC^C 830 GGGCCC AvaII 918 GGACC G^G[AT]CC 936 GGACC BglI 116 GCCGGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 457 GCCAAGACGGC BsaI 1280 GAGACC GGTCTC(1/5) 1084 GGTCTC BseRI 658 GAGGAG GAGGAG(10/8) 682 GAGGAG BsmAI 1280 GAGAC GTCTC(1/5) 1085 GTCTC BstEII 846 GGTCACC G^GT[ACGT]ACC 1231 GGTGACC BstPI 846 GGTCACC G^GT[ACGT]ACC 1231 GGTGACC BstUI 810 CGCG CG^CG 1305 CGCG BstXI 974 CCAATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1073 CCAAGGCACTGG CfoI 114 GCGC GCG^C 1270 GCGC DrdI 571 GACGACAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 879 GACAGAGATGTC EcoNI 534 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 599 CCTACCGGAGG EcoO65I 846 GGTCACC G^GT[ACGT]ACC 1231 GGTGACC FauI 497 GCGGG CCCGC(4/6) 640 GCGGG HhaI 114 GCGC GCG^C 1270 GCGC HinP1I 114 GCGC G^CGC 1270 GCGC HincII 99 GTCGAC GT[CT]^[GA]AC 1123 GTCGAC HindII 99 GTCGAC GT[CT]^[GA]AC 1123 GTCGAC MvnI 810 CGCG CG^CG 1305 CGCG NcoI 93 CCATGG C^CATGG 1133 CCATGG PinAI 767 ACCGGT A^CCGGT 938 ACCGGT PvuII 1048 CAGCTG CAG^CTG 1186 CAGCTG RsaI 723 GTAC GT^AC 1260 GTAC SalI 99 GTCGAC G^TCGAC 1123 GTCGAC ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AluI AspHI AspI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsaWI BsiEI BsiHKAI BsiYI BslI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I Cfr10I Cfr13I DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PleI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I