(Circular) (Six-base) MAPSORT of: pFN21AE2215.seq Check: 8324 from: 1 to: 6061 pFN21AE2215 6061 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2105 3129 3404 1937 Size: 168 1024 275 4594 Fragments arranged by size: 4594 1024 275 168 Acc65I G'GTAC_C Cuts at: 2727 3382 2727 Size: 655 5406 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2515 4124 5508 5776 1632 Size: 883 1609 1384 268 1917 Fragments arranged by size: 1917 1609 1384 883 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 2814 3267 4960 5937 1203 Size: 1611 453 1693 977 1327 Fragments arranged by size: 1693 1611 1327 977 453 AgeI A'CCGG_T Cuts at: 2772 2943 4892 2772 Size: 171 1949 3941 Fragments arranged by size: 3941 1949 171 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6061 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AlwNI CAG_nnn'CTG Cuts at: 3804 5376 3804 Size: 1572 4489 ApaI G_GGCC'C Cuts at: 2604 2839 2604 Size: 235 5826 ApaLI G'TGCA_C Cuts at: 4118 5274 4118 Size: 1156 4905 ApoI r'AATT_y Cuts at: 1493 2252 3370 3596 3748 5857 1493 Size: 759 1118 226 152 2109 1697 Fragments arranged by size: 2109 1697 1118 759 226 152 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 2832 3386 1870 Size: 73 889 554 4545 Fragments arranged by size: 4545 889 554 73 BamHI G'GATC_C Cuts at: 3391 3758 3391 Size: 367 5694 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2034 2384 2727 3382 4837 Size: 325 217 627 247 350 343 655 1455 Cuts at: 4837 618 Size: 1842 Fragments arranged by size: 1842 1455 655 627 350 343 325 247 217 BanII G_rGCy'C Cuts at: 1391 2539 2604 2839 3300 3380 1391 Size: 1148 65 235 461 80 4072 Fragments arranged by size: 4072 1148 461 235 80 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5090 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2335 2369 4252 4286 1903 Size: 34 398 34 1883 34 3678 Fragments arranged by size: 3678 1883 398 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2503 3953 5163 2503 Size: 1450 1210 3401 Fragments arranged by size: 3401 1450 1210 BclI T'GATC_A Cuts at: 1548 3171 1548 Size: 1623 4438 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2127 2468 Size: 107 122 71 631 605 337 118 341 Cuts at: 2468 4672 136 Size: 2204 1525 Fragments arranged by size: 2204 1525 631 605 341 337 122 118 107 71 BglII A'GATC_T Cuts at: 6056 6056 Size: 6061 BlpI GC'TnA_GC Cuts at: 3478 3478 Size: 6061 Bme1580I G_kGCm'C Cuts at: 1790 2387 2604 2839 4122 5278 1790 Size: 597 217 235 1283 1156 2573 Fragments arranged by size: 2573 1283 1156 597 235 217 BmgBI CAC'GTC Cuts at: 1318 2290 1318 Size: 972 5089 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3095 3792 4750 Size: 416 53 83 399 24 1678 697 958 Cuts at: 4750 442 Size: 1753 Fragments arranged by size: 1753 1678 958 697 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2279 3008 3024 3077 3325 4721 1239 Size: 1040 729 16 53 248 1396 2579 Fragments arranged by size: 2579 1396 1040 729 248 53 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4186 5051 5349 4186 Size: 865 298 4898 Fragments arranged by size: 4898 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3095 3279 4724 882 Size: 617 1596 184 1445 2219 Fragments arranged by size: 2219 1596 1445 617 184 BsaAI yAC'GTr Cuts at: 493 1204 3268 3852 493 Size: 711 2064 584 2702 Fragments arranged by size: 2702 2064 711 584 BsaBI GATnn'nnATC Cuts at: 6055 6055 Size: 6061 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3868 4250 4885 275 Size: 53 83 186 936 2335 382 635 1451 Fragments arranged by size: 2335 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2607 2625 2772 2943 4494 4892 5166 5313 5724 Size: 18 147 171 1551 398 274 147 411 Cuts at: 5724 2607 Size: 2944 Fragments arranged by size: 2944 1551 411 398 274 171 147 147 18 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2657 2687 2702 2732 3075 Size: 30 165 30 1254 30 15 30 343 Cuts at: 3075 3105 1178 Size: 30 4134 Fragments arranged by size: 4134 1254 343 165 30 30 30 30 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2678 2702 1186 Size: 1492 24 4545 Fragments arranged by size: 4545 1492 24 BseYI C'CCAG_C Cuts at: 2512 2616 2863 5264 5760 2512 Size: 104 247 2401 496 2813 Fragments arranged by size: 2813 2401 496 247 104 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2693 1163 Size: 573 957 4531 Fragments arranged by size: 4531 957 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2126 2431 2484 3511 4272 5300 Size: 275 186 125 305 53 1027 761 1028 Cuts at: 5300 1540 Size: 2301 Fragments arranged by size: 2301 1028 1027 761 305 275 186 125 53 BsiHKAI G_wGCw'C Cuts at: 2295 2904 3300 4122 4207 5278 2295 Size: 609 396 822 85 1071 3078 Fragments arranged by size: 3078 1071 822 609 396 85 BsmI GAATG_Cn' Cuts at: 3572 3665 3572 Size: 93 5968 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2295 2387 2539 2604 2839 2904 3300 Size: 399 505 92 152 65 235 65 396 Cuts at: 3300 3380 4122 4207 5278 1391 Size: 80 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 505 399 396 235 152 92 85 80 65 65 BspEI T'CCGG_A Cuts at: 2625 5724 2625 Size: 3099 2962 BspHI T'CATG_A Cuts at: 3950 5680 3950 Size: 1730 4331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3416 844 Size: 2572 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3948 1394 Size: 429 2125 3507 Fragments arranged by size: 3507 2125 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4556 4730 65 Size: 1183 3308 174 1396 Fragments arranged by size: 3308 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2121 2772 2943 3163 4705 4892 1700 Size: 110 311 651 171 220 1542 187 2869 Fragments arranged by size: 2869 1542 651 311 220 187 171 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6061 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 4121 5133 4121 Size: 1012 5049 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6061 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 2851 3236 1184 Size: 1667 385 4009 Fragments arranged by size: 4009 1667 385 BstXI CCAn_nnnn'nTGG Cuts at: 2986 3085 2986 Size: 99 5962 BstYI r'GATC_y Cuts at: 1972 2910 3391 3758 4143 4160 5612 5698 5710 Size: 938 481 367 385 17 1452 86 12 Cuts at: 5710 6056 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 938 481 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2045 2098 3138 513 Size: 552 939 41 53 1040 3436 Fragments arranged by size: 3436 1040 939 552 53 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5322 Fragments arranged by size: 5322 683 56 BtsI GCAGTG_nn' Cuts at: 2068 2741 3577 4371 4391 2068 Size: 673 836 794 20 3738 Fragments arranged by size: 3738 836 794 673 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6026 DraI TTT'AAA Cuts at: 3365 3365 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2582 2890 5068 809 Size: 507 1266 308 2178 1802 Fragments arranged by size: 2178 1802 1266 507 308 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2123 2428 2469 Size: 54 1425 50 275 18 293 305 41 Cuts at: 2469 2481 3135 3508 4397 6026 8 Size: 12 654 373 889 1629 43 Fragments arranged by size: 1629 1425 889 654 373 305 293 275 54 50 43 41 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2123 2428 2481 3508 1537 Size: 275 311 305 53 1027 4090 Fragments arranged by size: 4090 1027 311 305 275 53 EarI CTCTTCn'nnn_ Cuts at: 1353 2624 3991 1353 Size: 1271 1367 3423 Fragments arranged by size: 3423 1367 1271 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2145 4648 5022 5168 387 Size: 1064 138 556 2503 374 146 1280 Fragments arranged by size: 2503 1280 1064 556 374 146 138 EcoICRI GAG'CTC Cuts at: 3298 3298 Size: 6061 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2279 2515 3008 3024 3077 3325 4124 Size: 393 647 236 493 16 53 248 799 Cuts at: 4124 4721 5508 5776 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 799 787 647 597 493 393 268 248 236 53 16 EcoNI CCTnn'n_nnAGG Cuts at: 2543 2608 2543 Size: 65 5996 EcoO109I rG'GnC_Cy Cuts at: 2601 2613 2692 2836 2869 2601 Size: 12 79 144 33 5793 Fragments arranged by size: 5793 144 79 33 12 EcoRI G'AATT_C Cuts at: 3370 3748 3370 Size: 378 5683 FspI TGC'GCA Cuts at: 4567 4567 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 3278 5208 1678 Size: 1600 1930 2531 Fragments arranged by size: 2531 1930 1600 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2477 2509 4702 4734 4776 Size: 32 135 32 905 32 2193 32 42 Cuts at: 4776 4808 1373 Size: 32 2626 Fragments arranged by size: 2626 2193 905 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2106 3130 3405 3651 669 Size: 988 281 168 1024 275 246 3079 Fragments arranged by size: 3079 1024 988 281 275 246 168 HindIII A'AGCT_T Cuts at: 748 2366 748 Size: 1618 4443 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KpnI G_GTAC'C Cuts at: 2731 3386 2731 Size: 655 5406 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 2814 5937 2814 Size: 3123 2938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2874 5175 5359 1305 Size: 1569 2301 184 2007 Fragments arranged by size: 2301 2007 1569 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3137 10 Size: 54 1425 343 1305 2934 Fragments arranged by size: 2934 1425 1305 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2050 4019 4378 4537 518 Size: 648 884 1969 359 159 2042 Fragments arranged by size: 2042 1969 884 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2040 2525 3055 3193 3477 4154 5302 5547 Size: 434 485 530 138 284 677 1148 245 Cuts at: 5547 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 530 485 434 284 245 138 NaeI GCC'GGC Cuts at: 2123 2123 Size: 6061 NcoI C'CATG_G Cuts at: 513 1065 2004 2098 3138 513 Size: 552 939 94 1040 3436 Fragments arranged by size: 3436 1040 939 552 94 NdeI CA'TA_TG Cuts at: 387 387 Size: 6061 NgoMIV G'CCGG_C Cuts at: 2121 2121 Size: 6061 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 3419 4964 1132 Size: 97 2190 1545 2229 Fragments arranged by size: 2229 2190 1545 97 PasI CC'CwG_GG Cuts at: 1348 2545 2695 1348 Size: 1197 150 4714 Fragments arranged by size: 4714 1197 150 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 2551 1108 Size: 1443 4618 PfoI T'CCnGG_A Cuts at: 2295 2985 3324 2295 Size: 690 339 5032 Fragments arranged by size: 5032 690 339 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6061 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PspOMI G'GGCC_C Cuts at: 2600 2835 2600 Size: 235 5826 PstI C_TGCA'G Cuts at: 830 3413 830 Size: 2583 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 PvuII CAG'CTG Cuts at: 3055 3193 3055 Size: 138 5923 SacI G_AGCT'C Cuts at: 3300 3300 Size: 6061 SalI G'TCGA_C Cuts at: 1936 2104 3128 3403 1936 Size: 168 1024 275 4594 Fragments arranged by size: 4594 1024 275 168 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 4309 6021 4309 Size: 1712 4349 SfcI C'TryA_G Cuts at: 826 1045 3409 4544 5225 5416 826 Size: 219 2364 1135 681 191 1471 Fragments arranged by size: 2364 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 2834 3388 1872 Size: 962 554 4545 Fragments arranged by size: 4545 962 554 SmlI C'TyrA_G Cuts at: 820 1017 1943 4165 5066 5328 820 Size: 197 926 2222 901 262 1553 Fragments arranged by size: 2222 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 3985 5 Size: 47 3933 2081 Fragments arranged by size: 3933 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6061 StyI C'CwwG_G Cuts at: 513 1065 2004 2098 3078 3138 3500 513 Size: 552 939 94 980 60 362 3074 Fragments arranged by size: 3074 980 939 552 362 94 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4088 4105 4258 4443 4888 325 Size: 1255 2508 17 153 185 445 1498 Fragments arranged by size: 2508 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4307 6019 96 Size: 274 80 33 51 1443 2330 1712 138 Fragments arranged by size: 2330 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2702 2708 2734 2740 3075 3107 6021 6053 2702 Size: 6 26 6 335 32 2914 32 2710 Fragments arranged by size: 2914 2710 335 32 32 26 6 6 Tth111I GACn'n_nGTC Cuts at: 1316 2582 2849 3125 1316 Size: 1266 267 276 4252 Fragments arranged by size: 4252 1266 276 267 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3223 3223 Size: 6061 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 2832 3386 1870 Size: 962 554 4545 Fragments arranged by size: 4545 962 554 XmnI GAAnn'nnTTC Cuts at: 2744 4190 2744 Size: 1446 4615 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI