(Linear) (Six-base) MAPSORT of: KIEE2215.seq Check: 9681 from: 1 to: 1356 KIEE2215.orf With 182 enzymes: SgfI * July 15, 2010 18:55 .. AccI GT'mk_AC Cuts at: 0 100 1124 1356 Size: 100 1024 232 Fragments arranged by size: 1024 232 100 Acc65I G'GTAC_C Cuts at: 0 722 1356 Size: 722 634 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 510 1356 Size: 510 846 AflIII A'CryG_T Cuts at: 0 809 1262 1356 Size: 809 453 94 Fragments arranged by size: 809 453 94 AgeI A'CCGG_T Cuts at: 0 767 938 1356 Size: 767 171 418 Fragments arranged by size: 767 418 171 ApaI G_GGCC'C Cuts at: 0 599 834 1356 Size: 599 235 522 Fragments arranged by size: 599 522 235 ApoI r'AATT_y Cuts at: 0 247 1356 Size: 247 1109 AvaI C'yCGr_G Cuts at: 0 827 1356 Size: 827 529 BanI G'GyrC_C Cuts at: 0 29 379 722 1356 Size: 29 350 343 634 Fragments arranged by size: 634 350 343 29 BanII G_rGCy'C Cuts at: 0 534 599 834 1295 1356 Size: 534 65 235 461 61 Fragments arranged by size: 534 461 235 65 61 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 330 364 1356 Size: 330 34 992 Fragments arranged by size: 992 330 34 BciVI GTATCCnnnnn_n' Cuts at: 0 498 1356 Size: 498 858 BclI T'GATC_A Cuts at: 0 1166 1356 Size: 1166 190 BglI GCCn_nnn'nGGC Cuts at: 0 122 463 1356 Size: 122 341 893 Fragments arranged by size: 893 341 122 Bme1580I G_kGCm'C Cuts at: 0 382 599 834 1356 Size: 382 217 235 522 Fragments arranged by size: 522 382 235 217 BmgBI CAC'GTC Cuts at: 0 285 1356 Size: 285 1071 BmrI ACTGGGnnnn_n' Cuts at: 0 1090 1356 Size: 1090 266 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 274 1003 1019 1072 1320 1356 Size: 274 729 16 53 248 36 Fragments arranged by size: 729 274 248 53 36 16 BsaI GGTCTCn'nnnn_ Cuts at: 0 1090 1274 1356 Size: 1090 184 82 Fragments arranged by size: 1090 184 82 BsaAI yAC'GTr Cuts at: 0 1263 1356 Size: 1263 93 BsaWI w'CCGG_w Cuts at: 0 602 620 767 938 1356 Size: 602 18 147 171 418 Fragments arranged by size: 602 418 171 147 18 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 652 682 697 727 1070 1100 1356 Size: 652 30 15 30 343 30 256 Fragments arranged by size: 652 343 256 30 30 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 673 697 1356 Size: 673 24 659 Fragments arranged by size: 673 659 24 BseYI C'CCAG_C Cuts at: 0 507 611 858 1356 Size: 507 104 247 498 Fragments arranged by size: 507 498 247 104 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 688 1356 Size: 688 668 BsiEI CG_ry'CG Cuts at: 0 121 426 479 1356 Size: 121 305 53 877 Fragments arranged by size: 877 305 121 53 BsiHKAI G_wGCw'C Cuts at: 0 290 899 1295 1356 Size: 290 609 396 61 Fragments arranged by size: 609 396 290 61 Bsp1286I G_dGCh'C Cuts at: 0 290 382 534 599 834 899 1295 1356 Size: 290 92 152 65 235 65 396 61 Fragments arranged by size: 396 290 235 152 92 65 65 61 BspEI T'CCGG_A Cuts at: 0 620 1356 Size: 620 736 BsrFI r'CCGG_y Cuts at: 0 116 767 938 1158 1356 Size: 116 651 171 220 198 Fragments arranged by size: 651 220 198 171 116 BstEII G'GTnAC_C Cuts at: 0 846 1231 1356 Size: 846 385 125 Fragments arranged by size: 846 385 125 BstXI CCAn_nnnn'nTGG Cuts at: 0 981 1080 1356 Size: 981 99 276 Fragments arranged by size: 981 276 99 BstYI r'GATC_y Cuts at: 0 905 1356 Size: 905 451 BtgI C'CryG_G Cuts at: 0 40 93 1133 1356 Size: 40 53 1040 223 Fragments arranged by size: 1040 223 53 40 BtsI GCAGTG_nn' Cuts at: 0 63 736 1356 Size: 63 673 620 Fragments arranged by size: 673 620 63 DrdI GACnn_nn'nnGTC Cuts at: 0 577 885 1356 Size: 577 308 471 Fragments arranged by size: 577 471 308 EaeI y'GGCC_r Cuts at: 0 118 423 464 476 1130 1356 Size: 118 305 41 12 654 226 Fragments arranged by size: 654 305 226 118 41 12 EagI C'GGCC_G Cuts at: 0 118 423 476 1356 Size: 118 305 53 880 Fragments arranged by size: 880 305 118 53 EarI CTCTTCn'nnn_ Cuts at: 0 619 1356 Size: 619 737 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 140 1356 Size: 140 1216 EcoICRI GAG'CTC Cuts at: 0 1293 1356 Size: 1293 63 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 274 510 1003 1019 1072 1320 1356 Size: 274 236 493 16 53 248 36 Fragments arranged by size: 493 274 248 236 53 36 16 EcoNI CCTnn'n_nnAGG Cuts at: 0 538 603 1356 Size: 538 65 753 Fragments arranged by size: 753 538 65 EcoO109I rG'GnC_Cy Cuts at: 0 596 608 687 831 864 1356 Size: 596 12 79 144 33 492 Fragments arranged by size: 596 492 144 79 33 12 HaeII r_GCGC'y Cuts at: 0 1273 1356 Size: 1273 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 472 504 1356 Size: 472 32 852 Fragments arranged by size: 852 472 32 HincII GTy'rAC Cuts at: 0 101 1125 1356 Size: 101 1024 231 Fragments arranged by size: 1024 231 101 HindIII A'AGCT_T Cuts at: 0 361 1356 Size: 361 995 KpnI G_GTAC'C Cuts at: 0 726 1356 Size: 726 630 MluI A'CGCG_T Cuts at: 0 809 1356 Size: 809 547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 869 1356 Size: 869 487 MscI TGG'CCA Cuts at: 0 1132 1356 Size: 1132 224 MslI CAynn'nnrTG Cuts at: 0 45 1356 Size: 45 1311 MspA1I CmG'CkG Cuts at: 0 35 520 1050 1188 1356 Size: 35 485 530 138 168 Fragments arranged by size: 530 485 168 138 35 NaeI GCC'GGC Cuts at: 0 118 1356 Size: 118 1238 NcoI C'CATG_G Cuts at: 0 93 1133 1356 Size: 93 1040 223 Fragments arranged by size: 1040 223 93 NgoMIV G'CCGG_C Cuts at: 0 116 1356 Size: 116 1240 PasI CC'CwG_GG Cuts at: 0 540 690 1356 Size: 540 150 666 Fragments arranged by size: 666 540 150 PflMI CCAn_nnn'nTGG Cuts at: 0 546 1356 Size: 546 810 PfoI T'CCnGG_A Cuts at: 0 290 980 1319 1356 Size: 290 690 339 37 Fragments arranged by size: 690 339 290 37 PspOMI G'GGCC_C Cuts at: 0 595 830 1356 Size: 595 235 526 Fragments arranged by size: 595 526 235 PvuII CAG'CTG Cuts at: 0 1050 1188 1356 Size: 1050 138 168 Fragments arranged by size: 1050 168 138 SacI G_AGCT'C Cuts at: 0 1295 1356 Size: 1295 61 SalI G'TCGA_C Cuts at: 0 99 1123 1356 Size: 99 1024 233 Fragments arranged by size: 1024 233 99 SmaI CCC'GGG Cuts at: 0 829 1356 Size: 829 527 StyI C'CwwG_G Cuts at: 0 93 1073 1133 1356 Size: 93 980 60 223 Fragments arranged by size: 980 223 93 60 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 697 703 729 735 1070 1102 1356 Size: 697 6 26 6 335 32 254 Fragments arranged by size: 697 335 254 32 26 6 6 Tth111I GACn'n_nGTC Cuts at: 0 577 844 1120 1356 Size: 577 267 276 236 Fragments arranged by size: 577 276 267 236 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1218 1356 Size: 1218 138 XmaI C'CCGG_G Cuts at: 0 827 1356 Size: 827 529 XmnI GAAnn'nnTTC Cuts at: 0 739 1356 Size: 739 617 Enzymes that do cut: AccI Acc65I AcuI AflIII AgeI ApaI ApoI AvaI BanI BanII BcgI BciVI BclI BglI Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BsrFI BstEII BstXI BstYI BtgI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII KpnI MluI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI PspOMI PvuII SacI SalI SmaI StyI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BfrBI BglII BlpI BmtI BplI Bpu10I BpuEI BsaBI BsaHI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI MfeI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI XbaI XhoI ZraI