(Circular) (Six-base) MAPSORT of: pFN21AB7832.seq Check: 7341 from: 1 to: 6535 pFN21AB7832 6535 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4345 278 Size: 53 83 186 936 2809 2468 Fragments arranged by size: 2809 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3878 1937 Size: 1941 4594 Acc65I G'GTAC_C Cuts at: 3856 3856 Size: 6535 AclI AA'CG_TT Cuts at: 1563 4662 5035 1563 Size: 3099 373 3063 Fragments arranged by size: 3099 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4598 5982 6250 1632 Size: 2966 1384 268 1917 Fragments arranged by size: 2966 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6338 AflIII A'CryG_T Cuts at: 1203 2921 5434 6411 1203 Size: 1718 2513 977 1327 Fragments arranged by size: 2513 1718 1327 977 AgeI A'CCGG_T Cuts at: 5366 5366 Size: 6535 AhdI GACnn_n'nnGTC Cuts at: 5264 5264 Size: 6535 AleI CACnn'nnGTG Cuts at: 1166 2022 3006 1166 Size: 856 984 4695 Fragments arranged by size: 4695 984 856 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6535 AlwNI CAG_nnn'CTG Cuts at: 2230 4278 5850 2230 Size: 2048 1572 2915 Fragments arranged by size: 2915 2048 1572 ApaLI G'TGCA_C Cuts at: 2756 4592 5748 2756 Size: 1836 1156 3543 Fragments arranged by size: 3543 1836 1156 ApoI r'AATT_y Cuts at: 1493 2099 2286 3520 3541 3844 4070 4222 6331 Size: 606 187 1234 21 303 226 152 2109 Cuts at: 6331 1493 Size: 1697 Fragments arranged by size: 2109 1697 1234 606 303 226 187 152 21 AseI AT'TA_AT Cuts at: 160 1032 5089 160 Size: 872 4057 1606 Fragments arranged by size: 4057 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6535 AvaI C'yCGr_G Cuts at: 1870 1943 2708 3860 1870 Size: 73 765 1152 4545 Fragments arranged by size: 4545 1152 765 73 BamHI G'GATC_C Cuts at: 3865 4232 3865 Size: 367 6168 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3856 5311 618 Size: 325 217 627 2069 1455 1842 Fragments arranged by size: 2069 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3462 3854 1391 Size: 2071 392 4072 Fragments arranged by size: 4072 2071 392 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2426 3118 928 Size: 971 527 692 4345 Fragments arranged by size: 4345 971 692 527 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4726 4760 1903 Size: 34 50 34 2705 34 3678 Fragments arranged by size: 3678 2705 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3739 4427 5637 3739 Size: 688 1210 4637 Fragments arranged by size: 4637 1210 688 BclI T'GATC_A Cuts at: 1548 2194 1548 Size: 646 5889 BfrBI ATG'CAT Cuts at: 2624 3142 2624 Size: 518 6017 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5146 136 Size: 107 122 71 631 605 3474 1525 Fragments arranged by size: 3474 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6530 6530 Size: 6535 BlpI GC'TnA_GC Cuts at: 3952 3952 Size: 6535 Bme1580I G_kGCm'C Cuts at: 1790 2760 4596 5752 1790 Size: 970 1836 1156 2573 Fragments arranged by size: 2573 1836 1156 970 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6535 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2598 4266 5224 Size: 416 53 83 399 24 1181 1668 958 Cuts at: 5224 442 Size: 1753 Fragments arranged by size: 1753 1668 1181 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6535 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3294 3294 Size: 6535 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3339 3580 5195 1239 Size: 2100 241 1615 2579 Fragments arranged by size: 2579 2100 1615 241 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3194 3264 4660 5525 5823 3194 Size: 70 1396 865 298 3906 Fragments arranged by size: 3906 1396 865 298 70 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5198 882 Size: 617 3699 2219 Fragments arranged by size: 3699 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4326 493 Size: 711 3122 2702 Fragments arranged by size: 3122 2702 711 BsaBI GATnn'nnATC Cuts at: 6529 6529 Size: 6535 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4342 4724 5359 275 Size: 53 83 186 936 2809 382 635 1451 Fragments arranged by size: 2809 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4968 5366 5640 5787 6198 4968 Size: 398 274 147 411 5305 Fragments arranged by size: 5305 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3739 3769 1178 Size: 30 165 30 2336 30 3944 Fragments arranged by size: 3944 2336 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6535 BseYI C'CCAG_C Cuts at: 5738 6234 5738 Size: 496 6039 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3597 3621 1163 Size: 573 1861 24 4077 Fragments arranged by size: 4077 1861 573 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3985 4746 5774 1540 Size: 275 186 1984 761 1028 2301 Fragments arranged by size: 2301 1984 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2031 2760 2809 3462 4596 4681 5752 2031 Size: 729 49 653 1134 85 1071 2814 Fragments arranged by size: 2814 1134 1071 729 653 85 49 BsmI GAATG_Cn' Cuts at: 2626 4046 4139 2626 Size: 1420 93 5022 Fragments arranged by size: 5022 1420 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2760 2809 3462 3854 4596 4681 Size: 399 241 729 49 653 392 742 85 Cuts at: 4681 5752 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 729 653 399 392 241 85 49 BspEI T'CCGG_A Cuts at: 6198 6198 Size: 6535 BspHI T'CATG_A Cuts at: 4424 6154 4424 Size: 1730 4805 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2054 3890 844 Size: 1210 1836 3489 Fragments arranged by size: 3489 1836 1210 BsrBI CCG'CTC Cuts at: 1394 1823 4422 1394 Size: 429 2599 3507 Fragments arranged by size: 3507 2599 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5030 5204 65 Size: 1183 3782 174 1396 Fragments arranged by size: 3782 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5179 5366 1700 Size: 110 3369 187 2869 Fragments arranged by size: 3369 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6535 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6535 BssSI C'ACGA_G Cuts at: 4595 5607 4595 Size: 1012 5523 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6535 BstBI TT'CG_AA Cuts at: 5339 5339 Size: 6535 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6535 BstXI CCAn_nnnn'nTGG Cuts at: 3008 3008 Size: 6535 BstYI r'GATC_y Cuts at: 1972 3865 4232 4617 4634 6086 6172 6184 6530 Size: 1893 367 385 17 1452 86 12 346 Cuts at: 6530 1972 Size: 1977 Fragments arranged by size: 1977 1893 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3451 3451 Size: 6535 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5983 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5796 Fragments arranged by size: 5796 683 56 BtsI GCAGTG_nn' Cuts at: 4051 4845 4865 4051 Size: 794 20 5721 Fragments arranged by size: 5721 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6535 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6500 DraI TTT'AAA Cuts at: 2110 2259 3546 3839 2110 Size: 149 1287 293 4806 Fragments arranged by size: 4806 1287 293 149 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5542 809 Size: 507 4226 1802 Fragments arranged by size: 4226 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3982 4871 6500 Size: 54 1425 50 275 18 2152 889 1629 Cuts at: 6500 8 Size: 43 Fragments arranged by size: 2152 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3982 1537 Size: 275 2170 4090 Fragments arranged by size: 4090 2170 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2313 2871 3705 4465 1353 Size: 960 558 834 760 3423 Fragments arranged by size: 3423 960 834 760 558 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2876 5122 5496 5642 387 Size: 1064 138 1287 2246 374 146 1280 Fragments arranged by size: 2246 1287 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3460 3460 Size: 6535 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3339 3580 4598 5195 5982 6250 1239 Size: 393 1707 241 1018 597 787 268 1524 Fragments arranged by size: 1707 1524 1018 787 597 393 268 241 EcoNI CCTnn'n_nnAGG Cuts at: 2508 2508 Size: 6535 EcoRI G'AATT_C Cuts at: 2099 3844 4222 2099 Size: 1745 378 4412 Fragments arranged by size: 4412 1745 378 EcoRV GAT'ATC Cuts at: 2391 2391 Size: 6535 FspI TGC'GCA Cuts at: 5041 5041 Size: 6535 HaeII r_GCGC'y Cuts at: 1678 5682 1678 Size: 4004 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2609 2641 2702 2734 3486 Size: 32 135 32 1037 32 61 32 752 Cuts at: 3486 3518 3737 3769 5176 5208 5250 5282 1373 Size: 32 219 32 1407 32 42 32 2626 Fragments arranged by size: 2626 1407 1037 752 219 135 61 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2715 3783 3879 4125 669 Size: 988 281 777 1068 96 246 3079 Fragments arranged by size: 3079 1068 988 777 281 246 96 HindIII A'AGCT_T Cuts at: 748 748 Size: 6535 HpaI GTT'AAC Cuts at: 4125 4125 Size: 6535 KpnI G_GTAC'C Cuts at: 3860 3860 Size: 6535 MfeI C'AATT_G Cuts at: 4134 4134 Size: 6535 MluI A'CGCG_T Cuts at: 6411 6411 Size: 6535 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2214 2252 5649 5833 1305 Size: 909 38 3397 184 2007 Fragments arranged by size: 3397 2007 909 184 38 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4713 Fragments arranged by size: 4713 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2022 2121 3006 3063 3079 3147 3402 Size: 648 856 99 885 57 16 68 255 Cuts at: 3402 3418 3573 4493 4852 5011 518 Size: 16 155 920 359 159 2042 Fragments arranged by size: 2042 920 885 856 648 359 255 159 155 99 68 57 16 16 MspA1I CmG'CkG Cuts at: 1606 2056 3037 3951 4628 5776 6021 1606 Size: 450 981 914 677 1148 245 2120 Fragments arranged by size: 2120 1148 981 914 677 450 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5983 NdeI CA'TA_TG Cuts at: 387 3247 3419 387 Size: 2860 172 3503 Fragments arranged by size: 3503 2860 172 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6535 NotI GC'GGCC_GC Cuts at: 3982 3982 Size: 6535 NsiI A_TGCA'T Cuts at: 2626 3144 2626 Size: 518 6017 NspI r_CATG'y Cuts at: 1132 1229 2925 3050 3142 3730 3893 5438 1132 Size: 97 1696 125 92 588 163 1545 2229 Fragments arranged by size: 2229 1696 1545 588 163 125 97 92 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6535 PciI A'CATG_T Cuts at: 2921 5434 2921 Size: 2513 4022 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6535 PmeI GTTT'AAAC Cuts at: 3839 3839 Size: 6535 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4604 4636 6117 6149 4604 Size: 32 1481 32 4990 Fragments arranged by size: 4990 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6535 PsiI TTA'TAA Cuts at: 2454 4105 2454 Size: 1651 4884 PspXI vC'TCGA_Gb Cuts at: 2708 2708 Size: 6535 PstI C_TGCA'G Cuts at: 830 3887 830 Size: 3057 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6535 PvuII CAG'CTG Cuts at: 3037 3037 Size: 6535 SacI G_AGCT'C Cuts at: 3462 3462 Size: 6535 SalI G'TCGA_C Cuts at: 1936 3877 1936 Size: 1941 4594 SapI GCTCTTCn'nnn_ Cuts at: 2871 3705 2871 Size: 834 5701 SbfI CC_TGCA'GG Cuts at: 3887 3887 Size: 6535 ScaI AGT'ACT Cuts at: 4783 6495 4783 Size: 1712 4823 SfcI C'TryA_G Cuts at: 826 1045 2161 2382 2481 2667 3883 5018 5699 Size: 219 1116 221 99 186 1216 1135 681 Cuts at: 5699 5890 826 Size: 191 1471 Fragments arranged by size: 1471 1216 1135 1116 681 221 219 191 186 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6535 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6535 SmaI CCC'GGG Cuts at: 1872 3862 1872 Size: 1990 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2708 3209 3279 4639 5540 5802 Size: 197 926 765 501 70 1360 901 262 Cuts at: 5802 820 Size: 1553 Fragments arranged by size: 1553 1360 926 901 765 501 262 197 70 SnaBI TAC'GTA Cuts at: 493 4326 493 Size: 3833 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6535 SphI G_CATG'C Cuts at: 1132 3142 3893 1132 Size: 2010 751 3774 Fragments arranged by size: 3774 2010 751 SspI AAT'ATT Cuts at: 5 52 4459 5 Size: 47 4407 2081 Fragments arranged by size: 4407 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6535 StyI C'CwwG_G Cuts at: 513 1065 2898 3974 513 Size: 552 1833 1076 3074 Fragments arranged by size: 3074 1833 1076 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3465 4562 4579 4732 4917 5362 325 Size: 1255 1885 1097 17 153 185 445 1498 Fragments arranged by size: 1885 1498 1255 1097 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4781 6493 96 Size: 274 80 33 51 1443 2804 1712 138 Fragments arranged by size: 2804 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3217 3249 3461 3493 6495 6527 3217 Size: 32 212 32 3002 32 3225 Fragments arranged by size: 3225 3002 212 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6535 XbaI T'CTAG_A Cuts at: 3589 3871 3589 Size: 282 6253 XhoI C'TCGA_G Cuts at: 1943 2708 1943 Size: 765 5770 XmaI C'CCGG_G Cuts at: 1870 3860 1870 Size: 1990 4545 XmnI GAAnn'nnTTC Cuts at: 2170 2558 4664 2170 Size: 388 2106 4041 Fragments arranged by size: 4041 2106 388 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4343 276 Size: 53 83 186 936 2809 2468 Fragments arranged by size: 2809 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI