(Circular) (Six-base) MAPSORT of: pFN21AE1477.seq Check: 6382 from: 1 to: 5758 pFN21AE1477 5758 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3568 278 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3101 1937 Size: 1164 4594 Acc65I G'GTAC_C Cuts at: 3079 3079 Size: 5758 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2175 2315 2799 3821 5205 5473 1632 Size: 543 140 484 1022 1384 268 1917 Fragments arranged by size: 1917 1384 1022 543 484 268 140 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5561 AflIII A'CryG_T Cuts at: 1203 4657 5634 1203 Size: 3454 977 1327 Fragments arranged by size: 3454 1327 977 AgeI A'CCGG_T Cuts at: 4589 4589 Size: 5758 AhdI GACnn_n'nnGTC Cuts at: 4487 4487 Size: 5758 AleI CACnn'nnGTG Cuts at: 1166 2527 1166 Size: 1361 4397 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5758 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2898 2930 2898 Size: 32 5726 AlwNI CAG_nnn'CTG Cuts at: 3501 5073 3501 Size: 1572 4186 ApaLI G'TGCA_C Cuts at: 2560 3815 4971 2560 Size: 1255 1156 3347 Fragments arranged by size: 3347 1255 1156 ApoI r'AATT_y Cuts at: 1493 2021 2552 2805 3067 3293 3445 5554 1493 Size: 528 531 253 262 226 152 2109 1697 Fragments arranged by size: 2109 1697 531 528 262 253 226 152 AseI AT'TA_AT Cuts at: 160 1032 4312 160 Size: 872 3280 1606 Fragments arranged by size: 3280 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 2997 3083 1870 Size: 73 1054 86 4545 Fragments arranged by size: 4545 1054 86 73 BamHI G'GATC_C Cuts at: 3088 3455 3088 Size: 367 5391 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2108 2878 3079 4534 618 Size: 325 217 627 321 770 201 1455 1842 Fragments arranged by size: 1842 1455 770 627 325 321 217 201 BanII G_rGCy'C Cuts at: 1391 2764 3077 1391 Size: 1373 313 4072 Fragments arranged by size: 4072 1373 313 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2303 928 Size: 971 404 4383 Fragments arranged by size: 4383 971 404 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3949 3983 1903 Size: 34 2012 34 3678 Fragments arranged by size: 3678 2012 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4860 3650 Size: 1210 4548 BclI T'GATC_A Cuts at: 1548 2305 1548 Size: 757 5001 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4369 136 Size: 107 122 71 631 605 2697 1525 Fragments arranged by size: 2697 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2406 5753 2406 Size: 3347 2411 BlpI GC'TnA_GC Cuts at: 3175 3175 Size: 5758 Bme1580I G_kGCm'C Cuts at: 1790 2564 2957 3819 4975 1790 Size: 774 393 862 1156 2573 Fragments arranged by size: 2573 1156 862 774 393 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5758 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3489 4447 442 Size: 416 53 83 399 24 2072 958 1753 Fragments arranged by size: 2072 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5758 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2410 2684 2410 Size: 274 5484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2771 4418 1239 Size: 1532 1647 2579 Fragments arranged by size: 2579 1647 1532 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3883 4748 5046 3883 Size: 865 298 4595 Fragments arranged by size: 4595 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2398 4421 882 Size: 617 899 2023 2219 Fragments arranged by size: 2219 2023 899 617 BsaAI yAC'GTr Cuts at: 493 1204 3549 493 Size: 711 2345 2702 Fragments arranged by size: 2702 2345 711 BsaBI GATnn'nnATC Cuts at: 5752 5752 Size: 5758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3565 3947 4582 275 Size: 53 83 186 936 2032 382 635 1451 Fragments arranged by size: 2032 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4191 4589 4863 5010 5421 4191 Size: 398 274 147 411 4528 Fragments arranged by size: 4528 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5533 Fragments arranged by size: 5533 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2673 2740 2759 2841 1186 Size: 1487 67 19 82 4103 Fragments arranged by size: 4103 1487 82 67 19 BseYI C'CCAG_C Cuts at: 4961 5457 4961 Size: 496 5262 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2088 1163 Size: 573 352 4833 Fragments arranged by size: 4833 573 352 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3208 3969 4997 1540 Size: 275 186 1207 761 1028 2301 Fragments arranged by size: 2301 1207 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2564 3819 3904 4975 2564 Size: 1255 85 1071 3347 Fragments arranged by size: 3347 1255 1071 85 BsmI GAATG_Cn' Cuts at: 2095 3269 3362 2095 Size: 1174 93 4491 Fragments arranged by size: 4491 1174 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2564 2764 2957 3077 3819 3904 4975 Size: 399 774 200 193 120 742 85 1071 Cuts at: 4975 1391 Size: 2174 Fragments arranged by size: 2174 1071 774 742 399 200 193 120 85 BspEI T'CCGG_A Cuts at: 5421 5421 Size: 5758 BspHI T'CATG_A Cuts at: 3647 5377 3647 Size: 1730 4028 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3113 844 Size: 2269 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3645 1394 Size: 429 1822 3507 Fragments arranged by size: 3507 1822 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4253 4427 65 Size: 1183 3005 174 1396 Fragments arranged by size: 3005 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4402 4589 1700 Size: 110 2592 187 2869 Fragments arranged by size: 2869 2592 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5758 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5758 BssSI C'ACGA_G Cuts at: 3818 4830 3818 Size: 1012 4746 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5758 BstBI TT'CG_AA Cuts at: 4562 4562 Size: 5758 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5758 BstYI r'GATC_y Cuts at: 1972 2406 3088 3455 3840 3857 5309 5395 5407 Size: 434 682 367 385 17 1452 86 12 Cuts at: 5407 5753 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 682 434 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3019 513 Size: 552 1954 3252 Fragments arranged by size: 3252 1954 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5019 Fragments arranged by size: 5019 683 56 BtsI GCAGTG_nn' Cuts at: 2687 3274 4068 4088 2687 Size: 587 794 20 4357 Fragments arranged by size: 4357 794 587 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5723 DraI TTT'AAA Cuts at: 2266 3062 2266 Size: 796 4962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4765 809 Size: 507 3449 1802 Fragments arranged by size: 3449 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3205 4094 5723 Size: 54 1425 50 275 18 1375 889 1629 Cuts at: 5723 8 Size: 43 Fragments arranged by size: 1629 1425 1375 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3205 1537 Size: 275 1393 4090 Fragments arranged by size: 4090 1393 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2927 3688 1353 Size: 1574 761 3423 Fragments arranged by size: 3423 1574 761 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4345 4719 4865 387 Size: 1064 138 2756 374 146 1280 Fragments arranged by size: 2756 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2175 2315 2771 2799 3821 4418 5205 Size: 393 543 140 456 28 1022 597 787 Cuts at: 5205 5473 1239 Size: 268 1524 Fragments arranged by size: 1524 1022 787 597 543 456 393 268 140 28 EcoRI G'AATT_C Cuts at: 3067 3445 3067 Size: 378 5380 EcoRV GAT'ATC Cuts at: 2314 2314 Size: 5758 FspI TGC'GCA Cuts at: 4264 4264 Size: 5758 HaeII r_GCGC'y Cuts at: 1678 4905 1678 Size: 3227 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2290 2322 4399 4431 4473 Size: 32 135 32 718 32 2077 32 42 Cuts at: 4473 4505 1373 Size: 32 2626 Fragments arranged by size: 2626 2077 718 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3102 3348 669 Size: 988 281 1164 246 3079 Fragments arranged by size: 3079 1164 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5758 HpaI GTT'AAC Cuts at: 3348 3348 Size: 5758 KpnI G_GTAC'C Cuts at: 3083 3083 Size: 5758 MfeI C'AATT_G Cuts at: 3357 3357 Size: 5758 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2337 2711 4872 5056 1305 Size: 1032 374 2161 184 2007 Fragments arranged by size: 2161 2007 1032 374 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3936 Fragments arranged by size: 3936 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2527 3716 4075 4234 518 Size: 648 1361 1189 359 159 2042 Fragments arranged by size: 2042 1361 1189 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2610 3174 3851 4999 5244 1606 Size: 1004 564 677 1148 245 2120 Fragments arranged by size: 2120 1148 1004 677 564 245 NcoI C'CATG_G Cuts at: 513 1065 3019 513 Size: 552 1954 3252 Fragments arranged by size: 3252 1954 552 NdeI CA'TA_TG Cuts at: 387 2651 387 Size: 2264 3494 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5758 NotI GC'GGCC_GC Cuts at: 3205 3205 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 3116 4661 1132 Size: 97 1887 1545 2229 Fragments arranged by size: 2229 1887 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5758 PciI A'CATG_T Cuts at: 4657 4657 Size: 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 2861 1108 Size: 1753 4005 PfoI T'CCnGG_A Cuts at: 2411 2902 2411 Size: 491 5267 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3827 3859 5340 5372 3827 Size: 32 1481 32 4213 Fragments arranged by size: 4213 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5758 PsiI TTA'TAA Cuts at: 3328 3328 Size: 5758 PspXI vC'TCGA_Gb Cuts at: 2997 2997 Size: 5758 PstI C_TGCA'G Cuts at: 830 3110 830 Size: 2280 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 PvuII CAG'CTG Cuts at: 2610 2610 Size: 5758 SalI G'TCGA_C Cuts at: 1936 3100 1936 Size: 1164 4594 SbfI CC_TGCA'GG Cuts at: 3110 3110 Size: 5758 ScaI AGT'ACT Cuts at: 4006 5718 4006 Size: 1712 4046 SexAI A'CCwGG_T Cuts at: 2860 2860 Size: 5758 SfcI C'TryA_G Cuts at: 826 1045 3106 4241 4922 5113 826 Size: 219 2061 1135 681 191 1471 Fragments arranged by size: 2061 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5758 SmaI CCC'GGG Cuts at: 1872 3085 1872 Size: 1213 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2997 3862 4763 5025 820 Size: 197 926 1054 865 901 262 1553 Fragments arranged by size: 1553 1054 926 901 865 262 197 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5758 SphI G_CATG'C Cuts at: 1132 3116 1132 Size: 1984 3774 SspI AAT'ATT Cuts at: 5 52 2367 3682 5 Size: 47 2315 1315 2081 Fragments arranged by size: 2315 2081 1315 47 StuI AGG'CCT Cuts at: 1517 2938 1517 Size: 1421 4337 StyI C'CwwG_G Cuts at: 513 1065 2094 2112 3019 3197 513 Size: 552 1029 18 907 178 3074 Fragments arranged by size: 3074 1029 907 552 178 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2315 3785 3802 3955 4140 4585 325 Size: 1255 735 1470 17 153 185 445 1498 Fragments arranged by size: 1498 1470 1255 735 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4004 5716 96 Size: 274 80 33 51 1443 2027 1712 138 Fragments arranged by size: 2027 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2337 2369 5718 5750 2337 Size: 32 3349 32 2345 Fragments arranged by size: 3349 2345 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5758 XbaI T'CTAG_A Cuts at: 3094 3094 Size: 5758 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2409 2409 Size: 5758 XhoI C'TCGA_G Cuts at: 1943 2997 1943 Size: 1054 4704 XmaI C'CCGG_G Cuts at: 1870 3083 1870 Size: 1213 4545 XmnI GAAnn'nnTTC Cuts at: 3887 3887 Size: 5758 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3566 276 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI