################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI AlwI AocI Aor51HI ApaI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BsrBI BsrDI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MfeI MflI MluI MluNI MroI MscI MseI MunI NcoI NdeI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AlwNI 1045 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG AscI 654 GGCGCGCC GG^CGCGCC AvaI 508 CTCGAG C^[CT]CG[GA]G BanII 106 GGGCTC G[GA]GC[CT]^C BfaI 124 CTAG C^TAG BsaBI 336 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 15 CTCCTC GAGGAG(10/8) BsiEI 816 CGATCG CG[GA][CT]^CG BsmAI 233 GTCTC GTCTC(1/5) BsmBI 232 CGTCTC CGTCTC(1/5) BsoBI 508 CTCGAG C^[CT]CG[GA]G Bsp1407I 176 TGTACA T^GTACA BsrFI 856 GCCGGC [GA]^CCGG[CT] BsrGI 176 TGTACA T^GTACA BssSI 571 CTCGTG CACGAG(-5/-1) BstAPI 372 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 856 GCCGGC [GA]^CCGG[CT] DpnII 817 GATC ^GATC EarI 208 CTCTTC CTCTTC(1/4) Ksp632I 208 CTCTTC CTCTTC(1/4) MaeI 124 CTAG C^TAG MaeII 347 ACGT A^CGT MamI 336 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 817 GATC ^GATC NaeI 856 GCCGGC GCC^GGC NdeII 817 GATC ^GATC NgoMIV 856 GCCGGC G^CCGGC NheI 123 GCTAGC G^CTAGC PaeR7I 508 CTCGAG C^TCGAG PvuI 816 CGATCG CGAT^CG PvuII 321 CAGCTG CAG^CTG SapI 207 GCTCTTC GCTCTTC(1/4) Sau3AI 817 GATC ^GATC SfcI 84 CTACAG C^T[GA][CT]AG SmlI 508 CTCGAG C^T[CT][GA]AG SspBI 176 TGTACA T^GTACA TfiI 1105 GATTC G^A[AT]TC Tsp45I 1009 GTCAC ^GT[GC]AC XcmI 863 CCAACTGCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 508 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 179 ACATGT A^C[GA][CT]GT 311 ACATGT BanI 292 GGCGCC G^G[CT][GA]CC 859 GGCGCC BbeI 292 GGCGCC GGCGC^C 859 GGCGCC BsgI 70 CTGCAC GTGCAG(16/14) 250 CTGCAC BspLU11I 179 ACATGT A^CATGT 311 ACATGT BspMI 203 ACCTGC ACCTGC(4/8) 392 ACCTGC BsrI 257 ACTGG ACTGG(1/-1) 1040 CCAGT BstF5I 444 CATCC GGATG(2/0) 1032 GGATG FokI 444 CATCC GGATG(9/13) 1032 GGATG KasI 292 GGCGCC G^GCGCC 859 GGCGCC MaeIII 185 GTAAC ^GT[ACGT]AC 1009 GTCAC MslI 342 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 444 CATCCTGGTG NarI 292 GGCGCC GG^CGCC 859 GGCGCC NlaIII 180 CATG CATG^ 312 CATG NspI 179 ACATGT [GA]CATG^[CT] 311 ACATGT PleI 1066 GACTC GAGTC(4/5) 638 GAGTC PpuMI 503 GGGACCT [GA]G^G[AT]CC[CT] 952 AGGACCT SfoI 292 GGCGCC GGC^GCC 859 GGCGCC TaqI 509 TCGA T^CGA 527 TCGA TthHB8I 509 TCGA T^CGA 527 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI Alw44I ApaLI AspHI AvaII BbsI BbvI BcnI BmyI BpuAI BsaHI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII DraIII EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV RsaI Sau96I ScrFI SfaNI TseI TspRI