(Circular) (Six-base) MAPSORT of: pFN21AE3756.seq Check: 7563 from: 1 to: 5821 pFN21AE3756 5821 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2406 2406 Size: 5821 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3631 278 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2088 2628 2772 3164 1937 Size: 151 540 144 392 4594 Fragments arranged by size: 4594 540 392 151 144 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2524 3884 5268 5536 1632 Size: 892 1360 1384 268 1917 Fragments arranged by size: 1917 1384 1360 892 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 2184 2316 4720 5697 1203 Size: 981 132 2404 977 1327 Fragments arranged by size: 2404 1327 981 977 132 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 4550 4550 Size: 5821 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2585 2942 1184 Size: 1401 357 4063 Fragments arranged by size: 4063 1401 357 AlwNI CAG_nnn'CTG Cuts at: 3055 3564 5136 3055 Size: 509 1572 3740 Fragments arranged by size: 3740 1572 509 ApaLI G'TGCA_C Cuts at: 2162 2375 2480 3878 5034 2162 Size: 213 105 1398 1156 2949 Fragments arranged by size: 2949 1398 1156 213 105 ApoI r'AATT_y Cuts at: 1493 3130 3356 3508 5617 1493 Size: 1637 226 152 2109 1697 Fragments arranged by size: 2109 1697 1637 226 152 AscI GG'CGCG_CC Cuts at: 2660 2660 Size: 5821 AseI AT'TA_AT Cuts at: 160 1032 4375 160 Size: 872 3343 1606 Fragments arranged by size: 3343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 2513 3146 1870 Size: 73 570 633 4545 Fragments arranged by size: 4545 633 570 73 BamHI G'GATC_C Cuts at: 3151 3518 3151 Size: 367 5454 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2297 2864 3142 4597 618 Size: 325 217 627 510 567 278 1455 1842 Fragments arranged by size: 1842 1455 627 567 510 325 278 217 BanII G_rGCy'C Cuts at: 1391 2115 3140 1391 Size: 724 1025 4072 Fragments arranged by size: 4072 1025 724 BbeI G_GCGC'C Cuts at: 2301 2868 2301 Size: 567 5254 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2134 2638 2702 3078 928 Size: 971 235 504 64 376 3671 Fragments arranged by size: 3671 971 504 376 235 64 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4012 4046 1903 Size: 34 2075 34 3678 Fragments arranged by size: 3678 2075 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 1548 Size: 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4432 136 Size: 107 122 71 631 605 2760 1525 Fragments arranged by size: 2760 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5816 5816 Size: 5821 BlpI GC'TnA_GC Cuts at: 3238 3238 Size: 5821 Bme1580I G_kGCm'C Cuts at: 1790 2166 2379 2469 2484 2751 2942 3882 5038 Size: 376 213 90 15 267 191 940 1156 Cuts at: 5038 1790 Size: 2573 Fragments arranged by size: 2573 1156 940 376 267 213 191 90 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5821 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3552 4510 442 Size: 416 53 83 399 24 2135 958 1753 Fragments arranged by size: 2135 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2132 1056 Size: 1076 4745 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4481 1239 Size: 3242 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3946 4811 5109 3946 Size: 865 298 4658 Fragments arranged by size: 4658 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4484 882 Size: 617 2985 2219 Fragments arranged by size: 2985 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3612 493 Size: 711 2408 2702 Fragments arranged by size: 2702 2408 711 BsaBI GATnn'nnATC Cuts at: 2345 5815 2345 Size: 3470 2351 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2298 2670 2865 3024 Size: 53 83 186 936 765 372 195 159 Cuts at: 3024 3628 4010 4645 275 Size: 604 382 635 1451 Fragments arranged by size: 1451 936 765 635 604 382 372 195 186 159 83 53 BsaWI w'CCGG_w Cuts at: 4254 4652 4926 5073 5484 4254 Size: 398 274 147 411 4591 Fragments arranged by size: 4591 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5596 Fragments arranged by size: 5596 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2011 1186 Size: 825 4996 BseYI C'CCAG_C Cuts at: 2596 5024 5520 2596 Size: 2428 496 2897 Fragments arranged by size: 2897 2428 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2060 2240 1163 Size: 573 324 180 4744 Fragments arranged by size: 4744 573 324 180 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2824 3271 4032 5060 1540 Size: 275 186 823 447 761 1028 2301 Fragments arranged by size: 2301 1028 823 761 447 275 186 BsiHKAI G_wGCw'C Cuts at: 2166 2379 2484 3882 3967 5038 2166 Size: 213 105 1398 85 1071 2949 Fragments arranged by size: 2949 1398 1071 213 105 85 BsmI GAATG_Cn' Cuts at: 3332 3425 3332 Size: 93 5728 BsmBI CGTCTCn'nnnn_ Cuts at: 2243 2243 Size: 5821 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2166 2379 2469 2484 2751 2942 Size: 399 325 51 213 90 15 267 191 Cuts at: 2942 3140 3882 3967 5038 1391 Size: 198 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 325 267 213 198 191 90 85 51 15 BspEI T'CCGG_A Cuts at: 5484 5484 Size: 5821 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2217 2406 3176 844 Size: 1373 189 770 3489 Fragments arranged by size: 3489 1373 770 189 BsrBI CCG'CTC Cuts at: 1394 1823 3708 1394 Size: 429 1885 3507 Fragments arranged by size: 3507 1885 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4316 4490 65 Size: 1183 3068 174 1396 Fragments arranged by size: 3068 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2861 4465 4652 1700 Size: 110 1051 1604 187 2869 Fragments arranged by size: 2869 1604 1051 187 110 BsrGI T'GTAC_A Cuts at: 96 2181 96 Size: 2085 3736 BssHII G'CGCG_C Cuts at: 1925 2149 2151 2660 1925 Size: 224 2 509 5086 Fragments arranged by size: 5086 509 224 2 BssSI C'ACGA_G Cuts at: 2576 3881 4893 2576 Size: 1305 1012 3504 Fragments arranged by size: 3504 1305 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2383 794 Size: 1589 4232 BstBI TT'CG_AA Cuts at: 4625 4625 Size: 5821 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5821 BstYI r'GATC_y Cuts at: 1972 3151 3518 3903 3920 5372 5458 5470 5816 Size: 1179 367 385 17 1452 86 12 346 Cuts at: 5816 1972 Size: 1977 Fragments arranged by size: 1977 1452 1179 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5269 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5082 Fragments arranged by size: 5082 683 56 BtsI GCAGTG_nn' Cuts at: 2252 3337 4131 4151 2252 Size: 1085 794 20 3922 Fragments arranged by size: 3922 1085 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DraIII CAC_nnn'GTG Cuts at: 2083 2170 3099 2083 Size: 87 929 4805 Fragments arranged by size: 4805 929 87 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4828 809 Size: 507 3512 1802 Fragments arranged by size: 3512 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2586 2802 Size: 54 1425 50 275 18 369 387 216 Cuts at: 2802 2859 2964 3268 4157 5786 8 Size: 57 105 304 889 1629 43 Fragments arranged by size: 1629 1425 889 387 369 304 275 216 105 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3268 1537 Size: 275 1456 4090 Fragments arranged by size: 4090 1456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2219 3751 1353 Size: 866 1532 3423 Fragments arranged by size: 3423 1532 866 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2707 2998 3103 4408 4782 4928 Size: 1064 138 1118 291 105 1305 374 146 Cuts at: 4928 387 Size: 1280 Fragments arranged by size: 1305 1280 1118 1064 374 291 146 138 105 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2524 3884 4481 5268 5536 1239 Size: 393 892 1360 597 787 268 1524 Fragments arranged by size: 1524 1360 892 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2487 2509 2958 2487 Size: 22 449 5350 Fragments arranged by size: 5350 449 22 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 FspI TGC'GCA Cuts at: 4327 4327 Size: 5821 HaeII r_GCGC'y Cuts at: 1678 2301 2447 2597 2868 2907 2990 4968 1678 Size: 623 146 150 271 39 83 1978 2531 Fragments arranged by size: 2531 1978 623 271 150 146 83 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3062 3094 4462 4494 4536 Size: 32 135 32 1490 32 1368 32 42 Cuts at: 4536 4568 1373 Size: 32 2626 Fragments arranged by size: 2626 1490 1368 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3165 3411 669 Size: 988 281 1227 246 3079 Fragments arranged by size: 3079 1227 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5821 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KasI G'GCGC_C Cuts at: 2297 2864 2297 Size: 567 5254 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3085 3093 4935 5119 1305 Size: 1780 8 1842 184 2007 Fragments arranged by size: 2007 1842 1780 184 8 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3999 Fragments arranged by size: 3999 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2351 2453 3779 4138 4297 518 Size: 648 1185 102 1326 359 159 2042 Fragments arranged by size: 2042 1326 1185 648 359 159 102 MspA1I CmG'CkG Cuts at: 1606 2328 2383 2686 2887 3237 3914 5062 5307 Size: 722 55 303 201 350 677 1148 245 Cuts at: 5307 1606 Size: 2120 Fragments arranged by size: 2120 1148 722 677 350 303 245 201 55 NaeI GCC'GGC Cuts at: 2863 2863 Size: 5821 NarI GG'CG_CC Cuts at: 2298 2865 2298 Size: 567 5254 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5269 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NgoMIV G'CCGG_C Cuts at: 2861 2861 Size: 5821 NheI G'CTAG_C Cuts at: 1052 2128 1052 Size: 1076 4745 NotI GC'GGCC_GC Cuts at: 3268 3268 Size: 5821 NspI r_CATG'y Cuts at: 1132 1229 2188 2320 3179 4724 1132 Size: 97 959 132 859 1545 2229 Fragments arranged by size: 2229 1545 959 859 132 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5821 PciI A'CATG_T Cuts at: 2184 2316 4720 2184 Size: 132 2404 3285 Fragments arranged by size: 3285 2404 132 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5821 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2509 2958 2509 Size: 449 5372 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5821 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PspXI vC'TCGA_Gb Cuts at: 2513 2513 Size: 5821 PstI C_TGCA'G Cuts at: 830 3173 830 Size: 2343 3478 PvuI CG_AT'CG Cuts at: 2001 2824 2001 Size: 823 4998 PvuII CAG'CTG Cuts at: 2328 2328 Size: 5821 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SapI GCTCTTCn'nnn_ Cuts at: 2219 2219 Size: 5821 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 4069 5781 4069 Size: 1712 4109 SfcI C'TryA_G Cuts at: 826 1045 2089 3169 4304 4985 5176 826 Size: 219 1044 1080 1135 681 191 1471 Fragments arranged by size: 1471 1135 1080 1044 681 219 191 SfoI GGC'GCC Cuts at: 2299 2866 2299 Size: 567 5254 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 3148 1872 Size: 1276 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2513 3925 4826 5088 820 Size: 197 926 570 1412 901 262 1553 Fragments arranged by size: 1553 1412 926 901 570 262 197 SnaBI TAC'GTA Cuts at: 493 3612 493 Size: 3119 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 3179 1132 Size: 2047 3774 SspI AAT'ATT Cuts at: 5 52 3745 5 Size: 47 3693 2081 Fragments arranged by size: 3693 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5821 StyI C'CwwG_G Cuts at: 513 1065 3260 513 Size: 552 2195 3074 Fragments arranged by size: 3074 2195 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3848 3865 4018 4203 4648 325 Size: 1255 2268 17 153 185 445 1498 Fragments arranged by size: 2268 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2892 4067 Size: 274 80 33 51 1443 204 711 1175 Cuts at: 4067 5779 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1175 711 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3085 3117 5781 5813 3085 Size: 32 2664 32 3093 Fragments arranged by size: 3093 2664 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5821 XbaI T'CTAG_A Cuts at: 3157 3157 Size: 5821 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2875 2875 Size: 5821 XhoI C'TCGA_G Cuts at: 1943 2513 1943 Size: 570 5251 XmaI C'CCGG_G Cuts at: 1870 3146 1870 Size: 1276 4545 XmnI GAAnn'nnTTC Cuts at: 3950 3950 Size: 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3629 276 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI